The microorganism community of pentachlorophenol (PCP)-degrading coupled granules
Coupled granules are self-immobilized aggregates of microorganisms under micro aerobic conditions, which have the dissolved oxygen (DO) level of 0.6 mg.L(-1). The effects of DO concentration on pentachlorophenol (PCP) reduction and its microbial community were investigated in a coupled anaerobic and...
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| Veröffentlicht in: | Water science and technology 2009-01, Vol.59 (5), p.987-994 |
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| creator | Chen, Y C Chen, D Peng, L C Fu, S Y Zhan, H Y |
| description | Coupled granules are self-immobilized aggregates of microorganisms under micro aerobic conditions, which have the dissolved oxygen (DO) level of 0.6 mg.L(-1). The effects of DO concentration on pentachlorophenol (PCP) reduction and its microbial community were investigated in a coupled anaerobic and aerobic reactor. Both the diversity and the dynamicity of the Eubacteria and Archaea community, which were responsible for PCP degradation, were evaluated by means of amplification by polymerase chain reaction (PCR) and separation using the denaturing gradient gel electrophoresis (DGGE) technique. The results demonstrated a major shift in the Eubacteria and Archaea community as the mixed aerobic and anaerobic seeding sludge (1:1 by volume) developed into coupled granules and finally acclimated with PCP throughout the experiment period within 60 days. The numbers of the Eubacteria population decreased from 20, 16 to 11; Shannon diversity index decreased from 2.75, 2.53 to 2.10. In contrast, the number of the Archaea population increased from 12, 14 to 18; and Shannon diversity index increased from 1.87, 1.88 to 2.43. Phylogenetic analysis based on 16SrDNA genes showed the dominance Sphingomonas, Desulfobulbus, Proteobacteria, Actinobacterium, Methanogenic and some uncultured bacteria in the PCP-degrading coupled granules. Microorganism community construction of coupled granules was also deduced. |
| doi_str_mv | 10.2166/wst.2009.059 |
| format | Article |
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The effects of DO concentration on pentachlorophenol (PCP) reduction and its microbial community were investigated in a coupled anaerobic and aerobic reactor. Both the diversity and the dynamicity of the Eubacteria and Archaea community, which were responsible for PCP degradation, were evaluated by means of amplification by polymerase chain reaction (PCR) and separation using the denaturing gradient gel electrophoresis (DGGE) technique. The results demonstrated a major shift in the Eubacteria and Archaea community as the mixed aerobic and anaerobic seeding sludge (1:1 by volume) developed into coupled granules and finally acclimated with PCP throughout the experiment period within 60 days. The numbers of the Eubacteria population decreased from 20, 16 to 11; Shannon diversity index decreased from 2.75, 2.53 to 2.10. In contrast, the number of the Archaea population increased from 12, 14 to 18; and Shannon diversity index increased from 1.87, 1.88 to 2.43. Phylogenetic analysis based on 16SrDNA genes showed the dominance Sphingomonas, Desulfobulbus, Proteobacteria, Actinobacterium, Methanogenic and some uncultured bacteria in the PCP-degrading coupled granules. Microorganism community construction of coupled granules was also deduced.</description><identifier>ISSN: 0273-1223</identifier><identifier>EISSN: 1996-9732</identifier><identifier>DOI: 10.2166/wst.2009.059</identifier><identifier>PMID: 19273898</identifier><language>eng</language><publisher>England: IWA Publishing</publisher><subject>Aerobic conditions ; Aerobic microorganisms ; Anaerobic microorganisms ; Analytical methods ; Archaea ; Bacteria ; Biodegradation ; Biofilms ; Bioreactors - microbiology ; Communities ; Degradation ; Desulfobulbus ; Dissolved oxygen ; DNA ; DNA, Bacterial - genetics ; Eubacteria ; Gel electrophoresis ; Gels ; Genes ; Granular materials ; Microorganisms ; Nucleotide sequence ; Oxic conditions ; PCR ; Pentachlorophenol ; Pentachlorophenol - metabolism ; Phylogeny ; Polymerase chain reaction ; Population number ; Proteobacteria ; Reactors ; Sludge ; Sludge seeding ; Sphingomonas ; Wood preservatives</subject><ispartof>Water science and technology, 2009-01, Vol.59 (5), p.987-994</ispartof><rights>Copyright IWA Publishing Mar 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-d8544e61fe5bf4c361a524615cdc98eab6eb1c8b2edab9a7d62f0c9a54cb7a693</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19273898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Y C</creatorcontrib><creatorcontrib>Chen, D</creatorcontrib><creatorcontrib>Peng, L C</creatorcontrib><creatorcontrib>Fu, S Y</creatorcontrib><creatorcontrib>Zhan, H Y</creatorcontrib><title>The microorganism community of pentachlorophenol (PCP)-degrading coupled granules</title><title>Water science and technology</title><addtitle>Water Sci Technol</addtitle><description>Coupled granules are self-immobilized aggregates of microorganisms under micro aerobic conditions, which have the dissolved oxygen (DO) level of 0.6 mg.L(-1). The effects of DO concentration on pentachlorophenol (PCP) reduction and its microbial community were investigated in a coupled anaerobic and aerobic reactor. Both the diversity and the dynamicity of the Eubacteria and Archaea community, which were responsible for PCP degradation, were evaluated by means of amplification by polymerase chain reaction (PCR) and separation using the denaturing gradient gel electrophoresis (DGGE) technique. The results demonstrated a major shift in the Eubacteria and Archaea community as the mixed aerobic and anaerobic seeding sludge (1:1 by volume) developed into coupled granules and finally acclimated with PCP throughout the experiment period within 60 days. The numbers of the Eubacteria population decreased from 20, 16 to 11; Shannon diversity index decreased from 2.75, 2.53 to 2.10. In contrast, the number of the Archaea population increased from 12, 14 to 18; and Shannon diversity index increased from 1.87, 1.88 to 2.43. Phylogenetic analysis based on 16SrDNA genes showed the dominance Sphingomonas, Desulfobulbus, Proteobacteria, Actinobacterium, Methanogenic and some uncultured bacteria in the PCP-degrading coupled granules. Microorganism community construction of coupled granules was also deduced.</description><subject>Aerobic conditions</subject><subject>Aerobic microorganisms</subject><subject>Anaerobic microorganisms</subject><subject>Analytical methods</subject><subject>Archaea</subject><subject>Bacteria</subject><subject>Biodegradation</subject><subject>Biofilms</subject><subject>Bioreactors - microbiology</subject><subject>Communities</subject><subject>Degradation</subject><subject>Desulfobulbus</subject><subject>Dissolved oxygen</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Eubacteria</subject><subject>Gel electrophoresis</subject><subject>Gels</subject><subject>Genes</subject><subject>Granular materials</subject><subject>Microorganisms</subject><subject>Nucleotide sequence</subject><subject>Oxic conditions</subject><subject>PCR</subject><subject>Pentachlorophenol</subject><subject>Pentachlorophenol - metabolism</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Population number</subject><subject>Proteobacteria</subject><subject>Reactors</subject><subject>Sludge</subject><subject>Sludge seeding</subject><subject>Sphingomonas</subject><subject>Wood preservatives</subject><issn>0273-1223</issn><issn>1996-9732</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqN0U1LwzAYwPEgipvTm2cpCKJgZ96aNkcZvsHACfMc0vTpVmmbmrTIvr0ZGwhe9BRCfjyQ54_QOcFTSoS4-_L9lGIspziRB2hMpBSxTBk9RGNMUxYTStkInXj_gTFOGcfHaERkeMlkNkZvyzVETWWctW6l28o3kbFNM7RVv4lsGXXQ9tqsa-tst4bW1tH1Yra4iQtYOV1U7SrwoauhiMK9HWrwp-io1LWHs_05Qe-PD8vZczx_fXqZ3c9jw3nWx0WWcA6ClJDkJTdMEJ1QLkhiCiMz0LmAnJgsp1DoXOq0ELTERuqEmzzVQrIJutrN7Zz9HMD3qqm8gbrWLdjBK4ql4ISRf8CE8LCPPyHhmMitnKDLX_DDDq4Nv1UBMEGp4Dyo250Ky_XeQak6VzXabRTBaptOhXRqm06FdIFf7IcOeQPFD963Yt_FGpTg</recordid><startdate>20090101</startdate><enddate>20090101</enddate><creator>Chen, Y C</creator><creator>Chen, D</creator><creator>Peng, L C</creator><creator>Fu, S Y</creator><creator>Zhan, H Y</creator><general>IWA Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H96</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>L6V</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>7ST</scope><scope>SOI</scope><scope>7QL</scope><scope>7T7</scope><scope>7U6</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20090101</creationdate><title>The microorganism community of pentachlorophenol (PCP)-degrading coupled granules</title><author>Chen, Y C ; Chen, D ; Peng, L C ; Fu, S Y ; Zhan, H Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-d8544e61fe5bf4c361a524615cdc98eab6eb1c8b2edab9a7d62f0c9a54cb7a693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Aerobic conditions</topic><topic>Aerobic microorganisms</topic><topic>Anaerobic microorganisms</topic><topic>Analytical methods</topic><topic>Archaea</topic><topic>Bacteria</topic><topic>Biodegradation</topic><topic>Biofilms</topic><topic>Bioreactors - microbiology</topic><topic>Communities</topic><topic>Degradation</topic><topic>Desulfobulbus</topic><topic>Dissolved oxygen</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>Eubacteria</topic><topic>Gel electrophoresis</topic><topic>Gels</topic><topic>Genes</topic><topic>Granular materials</topic><topic>Microorganisms</topic><topic>Nucleotide sequence</topic><topic>Oxic conditions</topic><topic>PCR</topic><topic>Pentachlorophenol</topic><topic>Pentachlorophenol - metabolism</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Population number</topic><topic>Proteobacteria</topic><topic>Reactors</topic><topic>Sludge</topic><topic>Sludge seeding</topic><topic>Sphingomonas</topic><topic>Wood preservatives</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Y C</creatorcontrib><creatorcontrib>Chen, D</creatorcontrib><creatorcontrib>Peng, L C</creatorcontrib><creatorcontrib>Fu, S Y</creatorcontrib><creatorcontrib>Zhan, H Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Engineering Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Engineering Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Sustainability Science Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Water science and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Y C</au><au>Chen, D</au><au>Peng, L C</au><au>Fu, S Y</au><au>Zhan, H Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The microorganism community of pentachlorophenol (PCP)-degrading coupled granules</atitle><jtitle>Water science and technology</jtitle><addtitle>Water Sci Technol</addtitle><date>2009-01-01</date><risdate>2009</risdate><volume>59</volume><issue>5</issue><spage>987</spage><epage>994</epage><pages>987-994</pages><issn>0273-1223</issn><eissn>1996-9732</eissn><abstract>Coupled granules are self-immobilized aggregates of microorganisms under micro aerobic conditions, which have the dissolved oxygen (DO) level of 0.6 mg.L(-1). The effects of DO concentration on pentachlorophenol (PCP) reduction and its microbial community were investigated in a coupled anaerobic and aerobic reactor. Both the diversity and the dynamicity of the Eubacteria and Archaea community, which were responsible for PCP degradation, were evaluated by means of amplification by polymerase chain reaction (PCR) and separation using the denaturing gradient gel electrophoresis (DGGE) technique. The results demonstrated a major shift in the Eubacteria and Archaea community as the mixed aerobic and anaerobic seeding sludge (1:1 by volume) developed into coupled granules and finally acclimated with PCP throughout the experiment period within 60 days. The numbers of the Eubacteria population decreased from 20, 16 to 11; Shannon diversity index decreased from 2.75, 2.53 to 2.10. In contrast, the number of the Archaea population increased from 12, 14 to 18; and Shannon diversity index increased from 1.87, 1.88 to 2.43. Phylogenetic analysis based on 16SrDNA genes showed the dominance Sphingomonas, Desulfobulbus, Proteobacteria, Actinobacterium, Methanogenic and some uncultured bacteria in the PCP-degrading coupled granules. Microorganism community construction of coupled granules was also deduced.</abstract><cop>England</cop><pub>IWA Publishing</pub><pmid>19273898</pmid><doi>10.2166/wst.2009.059</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Water science and technology, 2009-01, Vol.59 (5), p.987-994 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Aerobic conditions Aerobic microorganisms Anaerobic microorganisms Analytical methods Archaea Bacteria Biodegradation Biofilms Bioreactors - microbiology Communities Degradation Desulfobulbus Dissolved oxygen DNA DNA, Bacterial - genetics Eubacteria Gel electrophoresis Gels Genes Granular materials Microorganisms Nucleotide sequence Oxic conditions PCR Pentachlorophenol Pentachlorophenol - metabolism Phylogeny Polymerase chain reaction Population number Proteobacteria Reactors Sludge Sludge seeding Sphingomonas Wood preservatives |
| title | The microorganism community of pentachlorophenol (PCP)-degrading coupled granules |
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