Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae
Aims The aim of this study was to evaluate the antagonistic activity of halotolerant bacteria against rice brown stripe pathogen Acidovorax oryzae. Methods and Results Fifteen of 136 isolates of halotolerant bacteria exhibited strong in vitro and in vivo antagonistic activity against both strains of...
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| Veröffentlicht in: | Journal of applied microbiology 2018-12, Vol.125 (6), p.1852-1867 |
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| container_title | Journal of applied microbiology |
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| creator | Masum, M.M.I. Liu, L. Yang, M. Hossain, M.M. Siddiqa, M.M. Supty, M.E. Ogunyemi, S.O. Hossain, A. An, Q. Li, B. |
| description | Aims
The aim of this study was to evaluate the antagonistic activity of halotolerant bacteria against rice brown stripe pathogen Acidovorax oryzae.
Methods and Results
Fifteen of 136 isolates of halotolerant bacteria exhibited strong in vitro and in vivo antagonistic activity against both strains of A. oryzae. The 15 antagonistic isolates were identified as ‘operational group Bacillus amyloliquefaciens’ based on physiological and biochemical features, fatty acid profiles as well as sequence analysis of 16S rRNA, gyrA and rpoB genes. Furthermore, this result indicated that the most effective antagonistic isolates K5‐3 and PPB6 could produce siderophore in iron‐limiting medium, and four kinds of secondary metabolites based on MALDI‐TOF analysis. In addition, the culture filtrates of isolates K5‐3 and PPB6 caused the damage of cell membrane evidenced by the TEM images, and resulted in 73–80% reduction in cell numbers, 55–65% reduction in biofilm formation, and 42–50% reduction in swimming ability of both strains of A. oryzae.
Conclusions
These isolates in particular K5‐3 and PPB6 of halotolerant bacteria markedly inhibited the growth of A. oryzae.
Significance and Impact of the Study
To our knowledge, this is the first report on biological control of halotolerant bacteria against bacterial brown stripe of rice. |
| doi_str_mv | 10.1111/jam.14088 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2094422590</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2094422590</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3538-4cf458eff030f97bc2084fae5d870d29abf7b12ea19292516935e855fde5c7383</originalsourceid><addsrcrecordid>eNp10ctu1DAUBuAIgWgpLHgBZIkNLNL6mstyWlEKKmID68hxjqceOT7BdijTt-CNMZ3CAglvbNmffh35r6qXjJ6yss52ej5lknbdo-qYiUbVvGn54_uzrBVt-VH1LKUdpUxQ1TytjgRlsmn67rj6eaU9ZvQQdchk1CZDdJqM4DFsXdiSjASX8podBu3JNuK6kHNtnPdrInree_Tu2wq2XEFIxAUyOjQYckRP0JJ8AyQ6A2SMeBtIytEtQBadb3ALgWyMm_A7Rv2DYNzfaXhePbHaJ3jxsJ9UXy_ffbm4qq8_v_9wsbmujVCiq6WxUnVgLRXU9u1oOO2k1aCmrqUT7_Vo25Fx0KznPVes6YWCTik7gTKt6MRJ9eaQu0Qs86c8zC4Z8F4HwDUNnPZScq56Wujrf-gO11i-oygmRMu4VLKotwdlIqYUwQ5LdLOO-4HR4XdPQ-lpuO-p2FcPies4w_RX_immgLMDuHUe9v9PGj5uPh0ifwFfJp9M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2133712454</pqid></control><display><type>article</type><title>Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Masum, M.M.I. ; Liu, L. ; Yang, M. ; Hossain, M.M. ; Siddiqa, M.M. ; Supty, M.E. ; Ogunyemi, S.O. ; Hossain, A. ; An, Q. ; Li, B.</creator><creatorcontrib>Masum, M.M.I. ; Liu, L. ; Yang, M. ; Hossain, M.M. ; Siddiqa, M.M. ; Supty, M.E. ; Ogunyemi, S.O. ; Hossain, A. ; An, Q. ; Li, B.</creatorcontrib><description>Aims
The aim of this study was to evaluate the antagonistic activity of halotolerant bacteria against rice brown stripe pathogen Acidovorax oryzae.
Methods and Results
Fifteen of 136 isolates of halotolerant bacteria exhibited strong in vitro and in vivo antagonistic activity against both strains of A. oryzae. The 15 antagonistic isolates were identified as ‘operational group Bacillus amyloliquefaciens’ based on physiological and biochemical features, fatty acid profiles as well as sequence analysis of 16S rRNA, gyrA and rpoB genes. Furthermore, this result indicated that the most effective antagonistic isolates K5‐3 and PPB6 could produce siderophore in iron‐limiting medium, and four kinds of secondary metabolites based on MALDI‐TOF analysis. In addition, the culture filtrates of isolates K5‐3 and PPB6 caused the damage of cell membrane evidenced by the TEM images, and resulted in 73–80% reduction in cell numbers, 55–65% reduction in biofilm formation, and 42–50% reduction in swimming ability of both strains of A. oryzae.
Conclusions
These isolates in particular K5‐3 and PPB6 of halotolerant bacteria markedly inhibited the growth of A. oryzae.
Significance and Impact of the Study
To our knowledge, this is the first report on biological control of halotolerant bacteria against bacterial brown stripe of rice.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14088</identifier><identifier>PMID: 30146698</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Acidovorax ; Acidovorax oryzae ; Bacillus ; Bacillus amyloliquefaciens ; Bacteria ; biocontrol ; Biofilms ; Biological control ; Cell culture ; Cell membranes ; Fatty acids ; halotolerant ; Iron ; lipopeptides ; MALDI‐TOF ; Metabolites ; Pathogens ; RpoB protein ; rRNA 16S ; Salinity tolerance ; Secondary metabolites ; siderophore ; Strains (organisms) ; Swimming</subject><ispartof>Journal of applied microbiology, 2018-12, Vol.125 (6), p.1852-1867</ispartof><rights>2018 The Society for Applied Microbiology</rights><rights>2018 The Society for Applied Microbiology.</rights><rights>Copyright © 2018 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-4cf458eff030f97bc2084fae5d870d29abf7b12ea19292516935e855fde5c7383</citedby><cites>FETCH-LOGICAL-c3538-4cf458eff030f97bc2084fae5d870d29abf7b12ea19292516935e855fde5c7383</cites><orcidid>0000-0002-4581-4775</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14088$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14088$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30146698$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Masum, M.M.I.</creatorcontrib><creatorcontrib>Liu, L.</creatorcontrib><creatorcontrib>Yang, M.</creatorcontrib><creatorcontrib>Hossain, M.M.</creatorcontrib><creatorcontrib>Siddiqa, M.M.</creatorcontrib><creatorcontrib>Supty, M.E.</creatorcontrib><creatorcontrib>Ogunyemi, S.O.</creatorcontrib><creatorcontrib>Hossain, A.</creatorcontrib><creatorcontrib>An, Q.</creatorcontrib><creatorcontrib>Li, B.</creatorcontrib><title>Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
The aim of this study was to evaluate the antagonistic activity of halotolerant bacteria against rice brown stripe pathogen Acidovorax oryzae.
Methods and Results
Fifteen of 136 isolates of halotolerant bacteria exhibited strong in vitro and in vivo antagonistic activity against both strains of A. oryzae. The 15 antagonistic isolates were identified as ‘operational group Bacillus amyloliquefaciens’ based on physiological and biochemical features, fatty acid profiles as well as sequence analysis of 16S rRNA, gyrA and rpoB genes. Furthermore, this result indicated that the most effective antagonistic isolates K5‐3 and PPB6 could produce siderophore in iron‐limiting medium, and four kinds of secondary metabolites based on MALDI‐TOF analysis. In addition, the culture filtrates of isolates K5‐3 and PPB6 caused the damage of cell membrane evidenced by the TEM images, and resulted in 73–80% reduction in cell numbers, 55–65% reduction in biofilm formation, and 42–50% reduction in swimming ability of both strains of A. oryzae.
Conclusions
These isolates in particular K5‐3 and PPB6 of halotolerant bacteria markedly inhibited the growth of A. oryzae.
Significance and Impact of the Study
To our knowledge, this is the first report on biological control of halotolerant bacteria against bacterial brown stripe of rice.</description><subject>Acidovorax</subject><subject>Acidovorax oryzae</subject><subject>Bacillus</subject><subject>Bacillus amyloliquefaciens</subject><subject>Bacteria</subject><subject>biocontrol</subject><subject>Biofilms</subject><subject>Biological control</subject><subject>Cell culture</subject><subject>Cell membranes</subject><subject>Fatty acids</subject><subject>halotolerant</subject><subject>Iron</subject><subject>lipopeptides</subject><subject>MALDI‐TOF</subject><subject>Metabolites</subject><subject>Pathogens</subject><subject>RpoB protein</subject><subject>rRNA 16S</subject><subject>Salinity tolerance</subject><subject>Secondary metabolites</subject><subject>siderophore</subject><subject>Strains (organisms)</subject><subject>Swimming</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp10ctu1DAUBuAIgWgpLHgBZIkNLNL6mstyWlEKKmID68hxjqceOT7BdijTt-CNMZ3CAglvbNmffh35r6qXjJ6yss52ej5lknbdo-qYiUbVvGn54_uzrBVt-VH1LKUdpUxQ1TytjgRlsmn67rj6eaU9ZvQQdchk1CZDdJqM4DFsXdiSjASX8podBu3JNuK6kHNtnPdrInree_Tu2wq2XEFIxAUyOjQYckRP0JJ8AyQ6A2SMeBtIytEtQBadb3ALgWyMm_A7Rv2DYNzfaXhePbHaJ3jxsJ9UXy_ffbm4qq8_v_9wsbmujVCiq6WxUnVgLRXU9u1oOO2k1aCmrqUT7_Vo25Fx0KznPVes6YWCTik7gTKt6MRJ9eaQu0Qs86c8zC4Z8F4HwDUNnPZScq56Wujrf-gO11i-oygmRMu4VLKotwdlIqYUwQ5LdLOO-4HR4XdPQ-lpuO-p2FcPies4w_RX_immgLMDuHUe9v9PGj5uPh0ifwFfJp9M</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Masum, M.M.I.</creator><creator>Liu, L.</creator><creator>Yang, M.</creator><creator>Hossain, M.M.</creator><creator>Siddiqa, M.M.</creator><creator>Supty, M.E.</creator><creator>Ogunyemi, S.O.</creator><creator>Hossain, A.</creator><creator>An, Q.</creator><creator>Li, B.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4581-4775</orcidid></search><sort><creationdate>201812</creationdate><title>Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae</title><author>Masum, M.M.I. ; Liu, L. ; Yang, M. ; Hossain, M.M. ; Siddiqa, M.M. ; Supty, M.E. ; Ogunyemi, S.O. ; Hossain, A. ; An, Q. ; Li, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-4cf458eff030f97bc2084fae5d870d29abf7b12ea19292516935e855fde5c7383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acidovorax</topic><topic>Acidovorax oryzae</topic><topic>Bacillus</topic><topic>Bacillus amyloliquefaciens</topic><topic>Bacteria</topic><topic>biocontrol</topic><topic>Biofilms</topic><topic>Biological control</topic><topic>Cell culture</topic><topic>Cell membranes</topic><topic>Fatty acids</topic><topic>halotolerant</topic><topic>Iron</topic><topic>lipopeptides</topic><topic>MALDI‐TOF</topic><topic>Metabolites</topic><topic>Pathogens</topic><topic>RpoB protein</topic><topic>rRNA 16S</topic><topic>Salinity tolerance</topic><topic>Secondary metabolites</topic><topic>siderophore</topic><topic>Strains (organisms)</topic><topic>Swimming</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Masum, M.M.I.</creatorcontrib><creatorcontrib>Liu, L.</creatorcontrib><creatorcontrib>Yang, M.</creatorcontrib><creatorcontrib>Hossain, M.M.</creatorcontrib><creatorcontrib>Siddiqa, M.M.</creatorcontrib><creatorcontrib>Supty, M.E.</creatorcontrib><creatorcontrib>Ogunyemi, S.O.</creatorcontrib><creatorcontrib>Hossain, A.</creatorcontrib><creatorcontrib>An, Q.</creatorcontrib><creatorcontrib>Li, B.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masum, M.M.I.</au><au>Liu, L.</au><au>Yang, M.</au><au>Hossain, M.M.</au><au>Siddiqa, M.M.</au><au>Supty, M.E.</au><au>Ogunyemi, S.O.</au><au>Hossain, A.</au><au>An, Q.</au><au>Li, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2018-12</date><risdate>2018</risdate><volume>125</volume><issue>6</issue><spage>1852</spage><epage>1867</epage><pages>1852-1867</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
The aim of this study was to evaluate the antagonistic activity of halotolerant bacteria against rice brown stripe pathogen Acidovorax oryzae.
Methods and Results
Fifteen of 136 isolates of halotolerant bacteria exhibited strong in vitro and in vivo antagonistic activity against both strains of A. oryzae. The 15 antagonistic isolates were identified as ‘operational group Bacillus amyloliquefaciens’ based on physiological and biochemical features, fatty acid profiles as well as sequence analysis of 16S rRNA, gyrA and rpoB genes. Furthermore, this result indicated that the most effective antagonistic isolates K5‐3 and PPB6 could produce siderophore in iron‐limiting medium, and four kinds of secondary metabolites based on MALDI‐TOF analysis. In addition, the culture filtrates of isolates K5‐3 and PPB6 caused the damage of cell membrane evidenced by the TEM images, and resulted in 73–80% reduction in cell numbers, 55–65% reduction in biofilm formation, and 42–50% reduction in swimming ability of both strains of A. oryzae.
Conclusions
These isolates in particular K5‐3 and PPB6 of halotolerant bacteria markedly inhibited the growth of A. oryzae.
Significance and Impact of the Study
To our knowledge, this is the first report on biological control of halotolerant bacteria against bacterial brown stripe of rice.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>30146698</pmid><doi>10.1111/jam.14088</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-4581-4775</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2018-12, Vol.125 (6), p.1852-1867 |
| issn | 1364-5072 1365-2672 |
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| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library Journals Frontfile Complete |
| subjects | Acidovorax Acidovorax oryzae Bacillus Bacillus amyloliquefaciens Bacteria biocontrol Biofilms Biological control Cell culture Cell membranes Fatty acids halotolerant Iron lipopeptides MALDI‐TOF Metabolites Pathogens RpoB protein rRNA 16S Salinity tolerance Secondary metabolites siderophore Strains (organisms) Swimming |
| title | Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae |
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