Knockdown of A‐kinase anchor protein 4 inhibits hypoxia‐induced epithelial‐to‐mesenchymal transition via suppression of the Wnt/β‐catenin pathway in human gastric cancer cells
Hypoxia induces epithelial‐mesenchymal transition (EMT) in tumorigenesis. A‐kinase anchor protein 4 (AKAP4) is a member of AKAPs family and plays a critical role in tumorigenesis. However, the biological role of AKAP4 in gastric cancer remains unknown. Thus, we investigated the effect of AKAP4 on EM...
Gespeichert in:
| Veröffentlicht in: | Journal of cellular biochemistry 2018-12, Vol.119 (12), p.10013-10020 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 10020 |
|---|---|
| container_issue | 12 |
| container_start_page | 10013 |
| container_title | Journal of cellular biochemistry |
| container_volume | 119 |
| creator | Li, Quanying Tang, Hongna Hu, Fangfang Qin, Changjiang |
| description | Hypoxia induces epithelial‐mesenchymal transition (EMT) in tumorigenesis. A‐kinase anchor protein 4 (AKAP4) is a member of AKAPs family and plays a critical role in tumorigenesis. However, the biological role of AKAP4 in gastric cancer remains unknown. Thus, we investigated the effect of AKAP4 on EMT in human gastric cancer cells under hypoxic conditions. Our results showed that AKAP4 expression was significantly upregulated in human gastric cancer cell lines. In addition, silenced expression of hypoxia‐inducible factor‐1α markedly suppressed AKAP4 expression in gastric cancer cells under hypoxia. Furthermore, knockdown of AKAP4 significantly prevented hypoxia‐induced migration, invasion, and EMT process in gastric cancer cells. Mechanistically, knockdown of AKAP4 prevented the activation of the Wnt/β‐catenin pathway in gastric cancer cells under hypoxia condition. These findings indicate that knockdown of AKAP4 inhibits hypoxia‐induced EMT in human gastric cancer cells, at least in part, via inactivation of the Wnt/β‐catenin signaling pathway. It is, therefore, AKAP4 may be a potential therapeutic target for the treatment of gastric cancer.
Knockdown of A‐kinase anchor protein 4 AKAP4 inhibits hypoxia‐induced epithelial‐mesenchymal transition in human gastric cancer cells, at least in part, via inactivation of the Wnt/β‐catenin signaling pathway. It is, therefore, AKAP4 may be a potential therapeutic target for the treatment of gastric cancer. |
| doi_str_mv | 10.1002/jcb.27331 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2094416987</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2129469475</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3531-1ac370b5ea4119e70d756f6de3e6abfb45032e1e0d11ca7707a3b6e985ec40143</originalsourceid><addsrcrecordid>eNp1kU2O1DAQhS0EYpqBBRdAltjAItN27MSd5dCC4WckNiCWUcWpEPckdrAdmuw4AmeZJYfgEJwENz2wQGJjy9ZX7z3VI-QhZ2ecsXy9081ZroTgt8iKs0plspTyNlkxJViWC56fkHsh7BhjVSXyu-REMC6LjShX5Psb6_RV6_aWuo6e__z67cpYCEjB6t55OnkX0VgqqbG9aUwMtF8m98VAQo1tZ40txcnEHgcDQ_qMLh0jBkwCywgDjR5sMNE4Sz8boGGeJo8hHN7JMg3SDzauf1ynMQ0RbXKbIPZ7WJIn7ecRLP0IIXqjqU6x0FONwxDukzsdDAEf3Nyn5P2L5--2L7PLtxevtueXmRaF4BkHLRRrCgTJeYWKtaoou7JFgSU0XSMLJnLkyFrONSjFFIimxGpToJZpT-KUPDnqpl18mjHEejThkAAsujnUOauk5GW1UQl9_A-6c7O3KV2d87ySZSVVkainR0p7F4LHrp68GcEvNWf1odA6FVr_LjSxj24U52bE9i_5p8EErI_A3gy4_F-pfr19dpT8BeKvs04</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2129469475</pqid></control><display><type>article</type><title>Knockdown of A‐kinase anchor protein 4 inhibits hypoxia‐induced epithelial‐to‐mesenchymal transition via suppression of the Wnt/β‐catenin pathway in human gastric cancer cells</title><source>Access via Wiley Online Library</source><source>MEDLINE</source><creator>Li, Quanying ; Tang, Hongna ; Hu, Fangfang ; Qin, Changjiang</creator><creatorcontrib>Li, Quanying ; Tang, Hongna ; Hu, Fangfang ; Qin, Changjiang</creatorcontrib><description>Hypoxia induces epithelial‐mesenchymal transition (EMT) in tumorigenesis. A‐kinase anchor protein 4 (AKAP4) is a member of AKAPs family and plays a critical role in tumorigenesis. However, the biological role of AKAP4 in gastric cancer remains unknown. Thus, we investigated the effect of AKAP4 on EMT in human gastric cancer cells under hypoxic conditions. Our results showed that AKAP4 expression was significantly upregulated in human gastric cancer cell lines. In addition, silenced expression of hypoxia‐inducible factor‐1α markedly suppressed AKAP4 expression in gastric cancer cells under hypoxia. Furthermore, knockdown of AKAP4 significantly prevented hypoxia‐induced migration, invasion, and EMT process in gastric cancer cells. Mechanistically, knockdown of AKAP4 prevented the activation of the Wnt/β‐catenin pathway in gastric cancer cells under hypoxia condition. These findings indicate that knockdown of AKAP4 inhibits hypoxia‐induced EMT in human gastric cancer cells, at least in part, via inactivation of the Wnt/β‐catenin signaling pathway. It is, therefore, AKAP4 may be a potential therapeutic target for the treatment of gastric cancer.
Knockdown of A‐kinase anchor protein 4 AKAP4 inhibits hypoxia‐induced epithelial‐mesenchymal transition in human gastric cancer cells, at least in part, via inactivation of the Wnt/β‐catenin signaling pathway. It is, therefore, AKAP4 may be a potential therapeutic target for the treatment of gastric cancer.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.27331</identifier><identifier>PMID: 30145836</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>A Kinase Anchor Proteins - metabolism ; A‐kinase anchor protein 4 (AKAP4) ; Cancer ; Catenin ; Cell Line, Tumor ; Cell Movement ; Deactivation ; Epithelial-Mesenchymal Transition ; epithelial‐to‐mesenchymal transition (EMT) ; Gastric cancer ; Gene Expression Regulation, Neoplastic ; Humans ; Hypoxia ; Inactivation ; Kinases ; Mesenchyme ; Proteins ; Signal transduction ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Therapeutic applications ; Tumor cell lines ; Tumorigenesis ; Wnt protein ; Wnt Signaling Pathway</subject><ispartof>Journal of cellular biochemistry, 2018-12, Vol.119 (12), p.10013-10020</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3531-1ac370b5ea4119e70d756f6de3e6abfb45032e1e0d11ca7707a3b6e985ec40143</citedby><cites>FETCH-LOGICAL-c3531-1ac370b5ea4119e70d756f6de3e6abfb45032e1e0d11ca7707a3b6e985ec40143</cites><orcidid>0000-0003-0363-2233</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.27331$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.27331$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30145836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Quanying</creatorcontrib><creatorcontrib>Tang, Hongna</creatorcontrib><creatorcontrib>Hu, Fangfang</creatorcontrib><creatorcontrib>Qin, Changjiang</creatorcontrib><title>Knockdown of A‐kinase anchor protein 4 inhibits hypoxia‐induced epithelial‐to‐mesenchymal transition via suppression of the Wnt/β‐catenin pathway in human gastric cancer cells</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>Hypoxia induces epithelial‐mesenchymal transition (EMT) in tumorigenesis. A‐kinase anchor protein 4 (AKAP4) is a member of AKAPs family and plays a critical role in tumorigenesis. However, the biological role of AKAP4 in gastric cancer remains unknown. Thus, we investigated the effect of AKAP4 on EMT in human gastric cancer cells under hypoxic conditions. Our results showed that AKAP4 expression was significantly upregulated in human gastric cancer cell lines. In addition, silenced expression of hypoxia‐inducible factor‐1α markedly suppressed AKAP4 expression in gastric cancer cells under hypoxia. Furthermore, knockdown of AKAP4 significantly prevented hypoxia‐induced migration, invasion, and EMT process in gastric cancer cells. Mechanistically, knockdown of AKAP4 prevented the activation of the Wnt/β‐catenin pathway in gastric cancer cells under hypoxia condition. These findings indicate that knockdown of AKAP4 inhibits hypoxia‐induced EMT in human gastric cancer cells, at least in part, via inactivation of the Wnt/β‐catenin signaling pathway. It is, therefore, AKAP4 may be a potential therapeutic target for the treatment of gastric cancer.
Knockdown of A‐kinase anchor protein 4 AKAP4 inhibits hypoxia‐induced epithelial‐mesenchymal transition in human gastric cancer cells, at least in part, via inactivation of the Wnt/β‐catenin signaling pathway. It is, therefore, AKAP4 may be a potential therapeutic target for the treatment of gastric cancer.</description><subject>A Kinase Anchor Proteins - metabolism</subject><subject>A‐kinase anchor protein 4 (AKAP4)</subject><subject>Cancer</subject><subject>Catenin</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Deactivation</subject><subject>Epithelial-Mesenchymal Transition</subject><subject>epithelial‐to‐mesenchymal transition (EMT)</subject><subject>Gastric cancer</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Hypoxia</subject><subject>Inactivation</subject><subject>Kinases</subject><subject>Mesenchyme</subject><subject>Proteins</subject><subject>Signal transduction</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Therapeutic applications</subject><subject>Tumor cell lines</subject><subject>Tumorigenesis</subject><subject>Wnt protein</subject><subject>Wnt Signaling Pathway</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU2O1DAQhS0EYpqBBRdAltjAItN27MSd5dCC4WckNiCWUcWpEPckdrAdmuw4AmeZJYfgEJwENz2wQGJjy9ZX7z3VI-QhZ2ecsXy9081ZroTgt8iKs0plspTyNlkxJViWC56fkHsh7BhjVSXyu-REMC6LjShX5Psb6_RV6_aWuo6e__z67cpYCEjB6t55OnkX0VgqqbG9aUwMtF8m98VAQo1tZ40txcnEHgcDQ_qMLh0jBkwCywgDjR5sMNE4Sz8boGGeJo8hHN7JMg3SDzauf1ynMQ0RbXKbIPZ7WJIn7ecRLP0IIXqjqU6x0FONwxDukzsdDAEf3Nyn5P2L5--2L7PLtxevtueXmRaF4BkHLRRrCgTJeYWKtaoou7JFgSU0XSMLJnLkyFrONSjFFIimxGpToJZpT-KUPDnqpl18mjHEejThkAAsujnUOauk5GW1UQl9_A-6c7O3KV2d87ySZSVVkainR0p7F4LHrp68GcEvNWf1odA6FVr_LjSxj24U52bE9i_5p8EErI_A3gy4_F-pfr19dpT8BeKvs04</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Li, Quanying</creator><creator>Tang, Hongna</creator><creator>Hu, Fangfang</creator><creator>Qin, Changjiang</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0363-2233</orcidid></search><sort><creationdate>201812</creationdate><title>Knockdown of A‐kinase anchor protein 4 inhibits hypoxia‐induced epithelial‐to‐mesenchymal transition via suppression of the Wnt/β‐catenin pathway in human gastric cancer cells</title><author>Li, Quanying ; Tang, Hongna ; Hu, Fangfang ; Qin, Changjiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3531-1ac370b5ea4119e70d756f6de3e6abfb45032e1e0d11ca7707a3b6e985ec40143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>A Kinase Anchor Proteins - metabolism</topic><topic>A‐kinase anchor protein 4 (AKAP4)</topic><topic>Cancer</topic><topic>Catenin</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Deactivation</topic><topic>Epithelial-Mesenchymal Transition</topic><topic>epithelial‐to‐mesenchymal transition (EMT)</topic><topic>Gastric cancer</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Hypoxia</topic><topic>Inactivation</topic><topic>Kinases</topic><topic>Mesenchyme</topic><topic>Proteins</topic><topic>Signal transduction</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Therapeutic applications</topic><topic>Tumor cell lines</topic><topic>Tumorigenesis</topic><topic>Wnt protein</topic><topic>Wnt Signaling Pathway</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Quanying</creatorcontrib><creatorcontrib>Tang, Hongna</creatorcontrib><creatorcontrib>Hu, Fangfang</creatorcontrib><creatorcontrib>Qin, Changjiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Quanying</au><au>Tang, Hongna</au><au>Hu, Fangfang</au><au>Qin, Changjiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Knockdown of A‐kinase anchor protein 4 inhibits hypoxia‐induced epithelial‐to‐mesenchymal transition via suppression of the Wnt/β‐catenin pathway in human gastric cancer cells</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2018-12</date><risdate>2018</risdate><volume>119</volume><issue>12</issue><spage>10013</spage><epage>10020</epage><pages>10013-10020</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>Hypoxia induces epithelial‐mesenchymal transition (EMT) in tumorigenesis. A‐kinase anchor protein 4 (AKAP4) is a member of AKAPs family and plays a critical role in tumorigenesis. However, the biological role of AKAP4 in gastric cancer remains unknown. Thus, we investigated the effect of AKAP4 on EMT in human gastric cancer cells under hypoxic conditions. Our results showed that AKAP4 expression was significantly upregulated in human gastric cancer cell lines. In addition, silenced expression of hypoxia‐inducible factor‐1α markedly suppressed AKAP4 expression in gastric cancer cells under hypoxia. Furthermore, knockdown of AKAP4 significantly prevented hypoxia‐induced migration, invasion, and EMT process in gastric cancer cells. Mechanistically, knockdown of AKAP4 prevented the activation of the Wnt/β‐catenin pathway in gastric cancer cells under hypoxia condition. These findings indicate that knockdown of AKAP4 inhibits hypoxia‐induced EMT in human gastric cancer cells, at least in part, via inactivation of the Wnt/β‐catenin signaling pathway. It is, therefore, AKAP4 may be a potential therapeutic target for the treatment of gastric cancer.
Knockdown of A‐kinase anchor protein 4 AKAP4 inhibits hypoxia‐induced epithelial‐mesenchymal transition in human gastric cancer cells, at least in part, via inactivation of the Wnt/β‐catenin signaling pathway. It is, therefore, AKAP4 may be a potential therapeutic target for the treatment of gastric cancer.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30145836</pmid><doi>10.1002/jcb.27331</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-0363-2233</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0730-2312 |
| ispartof | Journal of cellular biochemistry, 2018-12, Vol.119 (12), p.10013-10020 |
| issn | 0730-2312 1097-4644 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2094416987 |
| source | Access via Wiley Online Library; MEDLINE |
| subjects | A Kinase Anchor Proteins - metabolism A‐kinase anchor protein 4 (AKAP4) Cancer Catenin Cell Line, Tumor Cell Movement Deactivation Epithelial-Mesenchymal Transition epithelial‐to‐mesenchymal transition (EMT) Gastric cancer Gene Expression Regulation, Neoplastic Humans Hypoxia Inactivation Kinases Mesenchyme Proteins Signal transduction Stomach Neoplasms - genetics Stomach Neoplasms - metabolism Therapeutic applications Tumor cell lines Tumorigenesis Wnt protein Wnt Signaling Pathway |
| title | Knockdown of A‐kinase anchor protein 4 inhibits hypoxia‐induced epithelial‐to‐mesenchymal transition via suppression of the Wnt/β‐catenin pathway in human gastric cancer cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T13%3A16%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Knockdown%20of%20A%E2%80%90kinase%20anchor%20protein%204%20inhibits%20hypoxia%E2%80%90induced%20epithelial%E2%80%90to%E2%80%90mesenchymal%20transition%20via%20suppression%20of%20the%20Wnt/%CE%B2%E2%80%90catenin%20pathway%20in%20human%20gastric%20cancer%20cells&rft.jtitle=Journal%20of%20cellular%20biochemistry&rft.au=Li,%20Quanying&rft.date=2018-12&rft.volume=119&rft.issue=12&rft.spage=10013&rft.epage=10020&rft.pages=10013-10020&rft.issn=0730-2312&rft.eissn=1097-4644&rft_id=info:doi/10.1002/jcb.27331&rft_dat=%3Cproquest_cross%3E2129469475%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2129469475&rft_id=info:pmid/30145836&rfr_iscdi=true |