Role of AREA, a regulator of nitrogen metabolism, during colonization of maize kernels and fumonisin biosynthesis in Fusarium verticillioides
Fumonisin B1 (FB 1) biosynthesis is repressed in cultures containing ammonium as the nitrogen source and when grown on blister kernels, the earliest stages of kernel development. In this study AREA, a regulator of nitrogen metabolism, was disrupted in Fusarium verticilliodes. The mutant (ΔareA) grew...
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| Veröffentlicht in: | Fungal genetics and biology 2008-06, Vol.45 (6), p.947-953 |
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| creator | Kim, Hun Woloshuk, C.P. |
| description | Fumonisin B1 (FB
1) biosynthesis is repressed in cultures containing ammonium as the nitrogen source and when grown on blister kernels, the earliest stages of kernel development. In this study
AREA, a regulator of nitrogen metabolism, was disrupted in
Fusarium verticilliodes. The mutant (ΔareA) grew poorly on mature maize kernels, but grew similar to wild type (WT) with the addition of ammonium phosphate. FB
1 was not produced by ΔareA under any condition or by the WT with added ammonium phosphate. Constitutive expression of
AREA (strain AREA-CE) rescued the growth and FB
1 defects in ΔareA. Growth of WT,
ΔareA, and AREA-CE on blister-stage kernels was similar. After 7 days of growth, none of the strains produced FB
1 and the pH of the kernel tissues was 8.0. Addition of amylopectin to the blister kernels resulted in a pH near 6.6 and FB
1 production by WT and AREA-CE. The results support the hypothesis that FB
1 biosynthesis is regulated by AREA. Also the failure to produce FB
1 in blister kernels is due to high pH conditions generated because of an unfavorable carbon/nitrogen environment. |
| doi_str_mv | 10.1016/j.fgb.2008.03.007 |
| format | Article |
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1) biosynthesis is repressed in cultures containing ammonium as the nitrogen source and when grown on blister kernels, the earliest stages of kernel development. In this study
AREA, a regulator of nitrogen metabolism, was disrupted in
Fusarium verticilliodes. The mutant (ΔareA) grew poorly on mature maize kernels, but grew similar to wild type (WT) with the addition of ammonium phosphate. FB
1 was not produced by ΔareA under any condition or by the WT with added ammonium phosphate. Constitutive expression of
AREA (strain AREA-CE) rescued the growth and FB
1 defects in ΔareA. Growth of WT,
ΔareA, and AREA-CE on blister-stage kernels was similar. After 7 days of growth, none of the strains produced FB
1 and the pH of the kernel tissues was 8.0. Addition of amylopectin to the blister kernels resulted in a pH near 6.6 and FB
1 production by WT and AREA-CE. The results support the hypothesis that FB
1 biosynthesis is regulated by AREA. Also the failure to produce FB
1 in blister kernels is due to high pH conditions generated because of an unfavorable carbon/nitrogen environment.</description><identifier>ISSN: 1087-1845</identifier><identifier>EISSN: 1096-0937</identifier><identifier>DOI: 10.1016/j.fgb.2008.03.007</identifier><identifier>PMID: 18440841</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>ammonium phosphate ; Amylopectin ; AREA ; AREA protein ; biosynthesis ; corn ; Fumonisin ; fumonisin B1 ; Fumonisins - metabolism ; fungal diseases of plants ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fusarium - genetics ; Fusarium - growth & development ; Fusarium - metabolism ; Fusarium - pathogenicity ; Fusarium verticillioides ; Genes, Reporter ; Genetic Complementation Test ; Gibberella fujikuroi ; Hydrogen-Ion Concentration ; microbial colonization ; mutants ; Mycotoxin ; Nitrogen Compounds - metabolism ; nitrogen metabolism ; Nitrogen metabolite repression ; phenotype ; Plant Diseases - microbiology ; Promoter Regions, Genetic ; Quaternary Ammonium Compounds - metabolism ; Secondary metabolism ; seeds ; transcription factors ; Zea mays ; Zea mays - growth & development ; Zea mays - microbiology</subject><ispartof>Fungal genetics and biology, 2008-06, Vol.45 (6), p.947-953</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-41e950b08784dedc42bfcf30f6792e437a365d79391a9e18c359489e4e39e8083</citedby><cites>FETCH-LOGICAL-c406t-41e950b08784dedc42bfcf30f6792e437a365d79391a9e18c359489e4e39e8083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1087184508000431$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18440841$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hun</creatorcontrib><creatorcontrib>Woloshuk, C.P.</creatorcontrib><title>Role of AREA, a regulator of nitrogen metabolism, during colonization of maize kernels and fumonisin biosynthesis in Fusarium verticillioides</title><title>Fungal genetics and biology</title><addtitle>Fungal Genet Biol</addtitle><description>Fumonisin B1 (FB
1) biosynthesis is repressed in cultures containing ammonium as the nitrogen source and when grown on blister kernels, the earliest stages of kernel development. In this study
AREA, a regulator of nitrogen metabolism, was disrupted in
Fusarium verticilliodes. The mutant (ΔareA) grew poorly on mature maize kernels, but grew similar to wild type (WT) with the addition of ammonium phosphate. FB
1 was not produced by ΔareA under any condition or by the WT with added ammonium phosphate. Constitutive expression of
AREA (strain AREA-CE) rescued the growth and FB
1 defects in ΔareA. Growth of WT,
ΔareA, and AREA-CE on blister-stage kernels was similar. After 7 days of growth, none of the strains produced FB
1 and the pH of the kernel tissues was 8.0. Addition of amylopectin to the blister kernels resulted in a pH near 6.6 and FB
1 production by WT and AREA-CE. The results support the hypothesis that FB
1 biosynthesis is regulated by AREA. Also the failure to produce FB
1 in blister kernels is due to high pH conditions generated because of an unfavorable carbon/nitrogen environment.</description><subject>ammonium phosphate</subject><subject>Amylopectin</subject><subject>AREA</subject><subject>AREA protein</subject><subject>biosynthesis</subject><subject>corn</subject><subject>Fumonisin</subject><subject>fumonisin B1</subject><subject>Fumonisins - metabolism</subject><subject>fungal diseases of plants</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fusarium - genetics</subject><subject>Fusarium - growth & development</subject><subject>Fusarium - metabolism</subject><subject>Fusarium - pathogenicity</subject><subject>Fusarium verticillioides</subject><subject>Genes, Reporter</subject><subject>Genetic Complementation Test</subject><subject>Gibberella fujikuroi</subject><subject>Hydrogen-Ion Concentration</subject><subject>microbial colonization</subject><subject>mutants</subject><subject>Mycotoxin</subject><subject>Nitrogen Compounds - metabolism</subject><subject>nitrogen metabolism</subject><subject>Nitrogen metabolite repression</subject><subject>phenotype</subject><subject>Plant Diseases - microbiology</subject><subject>Promoter Regions, Genetic</subject><subject>Quaternary Ammonium Compounds - metabolism</subject><subject>Secondary metabolism</subject><subject>seeds</subject><subject>transcription factors</subject><subject>Zea mays</subject><subject>Zea mays - growth & development</subject><subject>Zea mays - microbiology</subject><issn>1087-1845</issn><issn>1096-0937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1TAQhSMEoqXwAGzAK1ZNGMdOYovVVdUfpEpIha4tx5mEuSR2sZNK7TvwziS6V2LHamaOvhnNzMmy9xwKDrz-vC_6oS1KAFWAKACaF9kpB13noEXzcstVk3Mlq5PsTUp7AM4ryV9nJ6smQUl-mv25CyOy0LPd3eXunFkWcVhGO4e4iZ7mGAb0bMLZtmGkNJ2zbonkB-bCGDw925mC39jJ0jOyXxg9jolZ37F-mVYikWcthfTk55-YKLG1vlqSjbRM7BHjTI7GkQJ1mN5mr3o7Jnx3jGfZ_dXlj4ub_Pbb9deL3W3uJNRzLjnqCtr1OiU77Jws2971Avq60SVK0VhRV12jheZWI1dOVFoqjRKFRgVKnGWfDnMfYvi9YJrNRMnhOFqPYUmmBC25FrCC_AC6GFKK2JuHSJONT4aD2Twwe7N6YDYPDAizerD2fDgOX9oJu38dx6evwMcD0Ntg7BApmfvvJXABvKor1WzElwOxvhIfCaNJjtA77Ciim00X6D8L_AX_K6JP</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Kim, Hun</creator><creator>Woloshuk, C.P.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20080601</creationdate><title>Role of AREA, a regulator of nitrogen metabolism, during colonization of maize kernels and fumonisin biosynthesis in Fusarium verticillioides</title><author>Kim, Hun ; Woloshuk, C.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-41e950b08784dedc42bfcf30f6792e437a365d79391a9e18c359489e4e39e8083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>ammonium phosphate</topic><topic>Amylopectin</topic><topic>AREA</topic><topic>AREA protein</topic><topic>biosynthesis</topic><topic>corn</topic><topic>Fumonisin</topic><topic>fumonisin B1</topic><topic>Fumonisins - metabolism</topic><topic>fungal diseases of plants</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Fusarium - genetics</topic><topic>Fusarium - growth & development</topic><topic>Fusarium - metabolism</topic><topic>Fusarium - pathogenicity</topic><topic>Fusarium verticillioides</topic><topic>Genes, Reporter</topic><topic>Genetic Complementation Test</topic><topic>Gibberella fujikuroi</topic><topic>Hydrogen-Ion Concentration</topic><topic>microbial colonization</topic><topic>mutants</topic><topic>Mycotoxin</topic><topic>Nitrogen Compounds - metabolism</topic><topic>nitrogen metabolism</topic><topic>Nitrogen metabolite repression</topic><topic>phenotype</topic><topic>Plant Diseases - microbiology</topic><topic>Promoter Regions, Genetic</topic><topic>Quaternary Ammonium Compounds - metabolism</topic><topic>Secondary metabolism</topic><topic>seeds</topic><topic>transcription factors</topic><topic>Zea mays</topic><topic>Zea mays - growth & development</topic><topic>Zea mays - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hun</creatorcontrib><creatorcontrib>Woloshuk, C.P.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Fungal genetics and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hun</au><au>Woloshuk, C.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of AREA, a regulator of nitrogen metabolism, during colonization of maize kernels and fumonisin biosynthesis in Fusarium verticillioides</atitle><jtitle>Fungal genetics and biology</jtitle><addtitle>Fungal Genet Biol</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>45</volume><issue>6</issue><spage>947</spage><epage>953</epage><pages>947-953</pages><issn>1087-1845</issn><eissn>1096-0937</eissn><abstract>Fumonisin B1 (FB
1) biosynthesis is repressed in cultures containing ammonium as the nitrogen source and when grown on blister kernels, the earliest stages of kernel development. In this study
AREA, a regulator of nitrogen metabolism, was disrupted in
Fusarium verticilliodes. The mutant (ΔareA) grew poorly on mature maize kernels, but grew similar to wild type (WT) with the addition of ammonium phosphate. FB
1 was not produced by ΔareA under any condition or by the WT with added ammonium phosphate. Constitutive expression of
AREA (strain AREA-CE) rescued the growth and FB
1 defects in ΔareA. Growth of WT,
ΔareA, and AREA-CE on blister-stage kernels was similar. After 7 days of growth, none of the strains produced FB
1 and the pH of the kernel tissues was 8.0. Addition of amylopectin to the blister kernels resulted in a pH near 6.6 and FB
1 production by WT and AREA-CE. The results support the hypothesis that FB
1 biosynthesis is regulated by AREA. Also the failure to produce FB
1 in blister kernels is due to high pH conditions generated because of an unfavorable carbon/nitrogen environment.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18440841</pmid><doi>10.1016/j.fgb.2008.03.007</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ammonium phosphate Amylopectin AREA AREA protein biosynthesis corn Fumonisin fumonisin B1 Fumonisins - metabolism fungal diseases of plants Fungal Proteins - genetics Fungal Proteins - metabolism Fusarium - genetics Fusarium - growth & development Fusarium - metabolism Fusarium - pathogenicity Fusarium verticillioides Genes, Reporter Genetic Complementation Test Gibberella fujikuroi Hydrogen-Ion Concentration microbial colonization mutants Mycotoxin Nitrogen Compounds - metabolism nitrogen metabolism Nitrogen metabolite repression phenotype Plant Diseases - microbiology Promoter Regions, Genetic Quaternary Ammonium Compounds - metabolism Secondary metabolism seeds transcription factors Zea mays Zea mays - growth & development Zea mays - microbiology |
| title | Role of AREA, a regulator of nitrogen metabolism, during colonization of maize kernels and fumonisin biosynthesis in Fusarium verticillioides |
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