Alpinetin inhibits proliferation and migration of ovarian cancer cells via suppression of STAT3 signaling
Natural bioactive components are increasingly being applied in cancer research. Alpinetin is a type of natural flavonoid primarily derived from Alpinia katsumadai Hayata, which exhibits anti‑bacterial and anti‑inflammatory properties. Therefore, it may possess anticancer potential and be employed th...
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| Veröffentlicht in: | Molecular medicine reports 2018-10, Vol.18 (4), p.4030-4036 |
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| description | Natural bioactive components are increasingly being applied in cancer research. Alpinetin is a type of natural flavonoid primarily derived from Alpinia katsumadai Hayata, which exhibits anti‑bacterial and anti‑inflammatory properties. Therefore, it may possess anticancer potential and be employed therapeutically for different diseases. The aim of the present study was to investigate the anticancer effects of alpinetin on the SKOV3 ovarian cancer cell line. The effect of alpinetin treatment on SKOV3 cell proliferation, apoptosis, spheroid and colony formation were measured using Cell Counting kit‑8, cell apoptosis, 3D spheroid and colony formation assays, respectively. Analysis of the cell cycle was performed using flow cytometry. Western blot analysis was used to determine the protein expression levels of B‑cell lymphoma (Bcl)‑2, Bcl‑2‑associated X protein, cleaved caspase‑3, cleaved poly (ADP‑ribose) polymerase (PARP), cyclin D1, cyclin‑dependent kinase (CDK) 4, CDK6, signal transducer and activator of transcription (STAT) 3, phosphorylated (p)‑STAT3, c‑myc, survivin, tissue inhibitor of metalloproteinase (TIMP)‑1, TIMP‑2, matrix metalloproteinase (MMP)‑2 and MMP‑9. In addition, a wound healing assay was used to determine cancer cell migration. The results revealed alpinetin suppressed cell viability and induced apoptosis of SKOV3 cells in a dose‑ and time‑dependent manner, and cells were arrested in the G1 phase. Alpinetin treatment upregulated protein expression levels of Bax, cleaved caspase‑3 and PARP, and downregulated protein expression levels of Bcl‑2, cyclin D1, CDK4 and CDK6. Alpinetin also inhibited cell migration, through increased protein expression levels of TIMP‑1 and TIMP‑2, and decreased protein expression levels of MMP‑2 and MMP‑9. Alpinetin also significantly suppressed colony and spheroid formation by SKOV3 cells. In addition, the STAT3 pathway was suppressed as demonstrated by downregulation of p‑STAT3 and reduced expression of downstream factors, including c‑myc and survivin. Overall, these results indicated that alpinetin may have anticancer effects on human ovarian cancer by inhibiting the STAT3 signaling pathway. |
| doi_str_mv | 10.3892/mmr.2018.9420 |
| format | Article |
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Alpinetin is a type of natural flavonoid primarily derived from Alpinia katsumadai Hayata, which exhibits anti‑bacterial and anti‑inflammatory properties. Therefore, it may possess anticancer potential and be employed therapeutically for different diseases. The aim of the present study was to investigate the anticancer effects of alpinetin on the SKOV3 ovarian cancer cell line. The effect of alpinetin treatment on SKOV3 cell proliferation, apoptosis, spheroid and colony formation were measured using Cell Counting kit‑8, cell apoptosis, 3D spheroid and colony formation assays, respectively. Analysis of the cell cycle was performed using flow cytometry. Western blot analysis was used to determine the protein expression levels of B‑cell lymphoma (Bcl)‑2, Bcl‑2‑associated X protein, cleaved caspase‑3, cleaved poly (ADP‑ribose) polymerase (PARP), cyclin D1, cyclin‑dependent kinase (CDK) 4, CDK6, signal transducer and activator of transcription (STAT) 3, phosphorylated (p)‑STAT3, c‑myc, survivin, tissue inhibitor of metalloproteinase (TIMP)‑1, TIMP‑2, matrix metalloproteinase (MMP)‑2 and MMP‑9. In addition, a wound healing assay was used to determine cancer cell migration. The results revealed alpinetin suppressed cell viability and induced apoptosis of SKOV3 cells in a dose‑ and time‑dependent manner, and cells were arrested in the G1 phase. Alpinetin treatment upregulated protein expression levels of Bax, cleaved caspase‑3 and PARP, and downregulated protein expression levels of Bcl‑2, cyclin D1, CDK4 and CDK6. Alpinetin also inhibited cell migration, through increased protein expression levels of TIMP‑1 and TIMP‑2, and decreased protein expression levels of MMP‑2 and MMP‑9. Alpinetin also significantly suppressed colony and spheroid formation by SKOV3 cells. In addition, the STAT3 pathway was suppressed as demonstrated by downregulation of p‑STAT3 and reduced expression of downstream factors, including c‑myc and survivin. Overall, these results indicated that alpinetin may have anticancer effects on human ovarian cancer by inhibiting the STAT3 signaling pathway.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2018.9420</identifier><identifier>PMID: 30132572</identifier><language>eng</language><publisher>Greece: Spandidos Publications UK Ltd</publisher><subject>Anti-inflammatory agents ; Antineoplastic Agents, Phytogenic - chemistry ; Antineoplastic Agents, Phytogenic - pharmacology ; Apoptosis ; Apoptosis - drug effects ; B-cell lymphoma ; Bax protein ; Bcl-2 protein ; Biotechnology ; c-Myc protein ; Cancer therapies ; Caspase ; Caspase-3 ; Cell adhesion & migration ; Cell cycle ; Cell Cycle Proteins - metabolism ; Cell growth ; Cell Line, Tumor ; Cell migration ; Cell Movement - drug effects ; Cell proliferation ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Chemotherapy ; Colonies ; Cyclin D1 ; Cyclin-dependent kinase 4 ; Cyclin-dependent kinases ; Female ; Flavanones - chemistry ; Flavanones - pharmacology ; Flow cytometry ; G1 phase ; G1 Phase - drug effects ; Gelatinase A ; Humans ; Immunoglobulins ; Inflammation ; Kinases ; Lung cancer ; Lymphocytes B ; Matrix Metalloproteinases - metabolism ; Metastasis ; Ovarian cancer ; Ovarian Neoplasms - enzymology ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - pathology ; Poly(ADP-ribose) polymerase ; Protein expression ; Proteins ; S Phase - drug effects ; Signal transduction ; Signal Transduction - drug effects ; Spheroids, Cellular - drug effects ; Spheroids, Cellular - metabolism ; Spheroids, Cellular - pathology ; STAT3 Transcription Factor - metabolism ; Studies ; Tissue Inhibitor of Metalloproteinases ; Transcription ; Tumor Stem Cell Assay ; Tumorigenesis ; Wound healing</subject><ispartof>Molecular medicine reports, 2018-10, Vol.18 (4), p.4030-4036</ispartof><rights>Copyright Spandidos Publications UK Ltd. 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-83e69c1b7bcc0e2ebd53152ba7a376436d096fbcbf763517f55713cabfaa72f83</citedby><cites>FETCH-LOGICAL-c360t-83e69c1b7bcc0e2ebd53152ba7a376436d096fbcbf763517f55713cabfaa72f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30132572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Xuezhi</creatorcontrib><creatorcontrib>Guo, Xiaohan</creatorcontrib><creatorcontrib>Shen, Junhua</creatorcontrib><creatorcontrib>Hua, Dingchao</creatorcontrib><title>Alpinetin inhibits proliferation and migration of ovarian cancer cells via suppression of STAT3 signaling</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Natural bioactive components are increasingly being applied in cancer research. Alpinetin is a type of natural flavonoid primarily derived from Alpinia katsumadai Hayata, which exhibits anti‑bacterial and anti‑inflammatory properties. Therefore, it may possess anticancer potential and be employed therapeutically for different diseases. The aim of the present study was to investigate the anticancer effects of alpinetin on the SKOV3 ovarian cancer cell line. The effect of alpinetin treatment on SKOV3 cell proliferation, apoptosis, spheroid and colony formation were measured using Cell Counting kit‑8, cell apoptosis, 3D spheroid and colony formation assays, respectively. Analysis of the cell cycle was performed using flow cytometry. Western blot analysis was used to determine the protein expression levels of B‑cell lymphoma (Bcl)‑2, Bcl‑2‑associated X protein, cleaved caspase‑3, cleaved poly (ADP‑ribose) polymerase (PARP), cyclin D1, cyclin‑dependent kinase (CDK) 4, CDK6, signal transducer and activator of transcription (STAT) 3, phosphorylated (p)‑STAT3, c‑myc, survivin, tissue inhibitor of metalloproteinase (TIMP)‑1, TIMP‑2, matrix metalloproteinase (MMP)‑2 and MMP‑9. In addition, a wound healing assay was used to determine cancer cell migration. The results revealed alpinetin suppressed cell viability and induced apoptosis of SKOV3 cells in a dose‑ and time‑dependent manner, and cells were arrested in the G1 phase. Alpinetin treatment upregulated protein expression levels of Bax, cleaved caspase‑3 and PARP, and downregulated protein expression levels of Bcl‑2, cyclin D1, CDK4 and CDK6. Alpinetin also inhibited cell migration, through increased protein expression levels of TIMP‑1 and TIMP‑2, and decreased protein expression levels of MMP‑2 and MMP‑9. Alpinetin also significantly suppressed colony and spheroid formation by SKOV3 cells. In addition, the STAT3 pathway was suppressed as demonstrated by downregulation of p‑STAT3 and reduced expression of downstream factors, including c‑myc and survivin. Overall, these results indicated that alpinetin may have anticancer effects on human ovarian cancer by inhibiting the STAT3 signaling pathway.</description><subject>Anti-inflammatory agents</subject><subject>Antineoplastic Agents, Phytogenic - chemistry</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>B-cell lymphoma</subject><subject>Bax protein</subject><subject>Bcl-2 protein</subject><subject>Biotechnology</subject><subject>c-Myc protein</subject><subject>Cancer therapies</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>Cell adhesion & migration</subject><subject>Cell cycle</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Chemotherapy</subject><subject>Colonies</subject><subject>Cyclin D1</subject><subject>Cyclin-dependent kinase 4</subject><subject>Cyclin-dependent kinases</subject><subject>Female</subject><subject>Flavanones - chemistry</subject><subject>Flavanones - pharmacology</subject><subject>Flow cytometry</subject><subject>G1 phase</subject><subject>G1 Phase - drug effects</subject><subject>Gelatinase A</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>Inflammation</subject><subject>Kinases</subject><subject>Lung cancer</subject><subject>Lymphocytes B</subject><subject>Matrix Metalloproteinases - metabolism</subject><subject>Metastasis</subject><subject>Ovarian cancer</subject><subject>Ovarian Neoplasms - enzymology</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Poly(ADP-ribose) polymerase</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>S Phase - drug effects</subject><subject>Signal transduction</subject><subject>Signal Transduction - drug effects</subject><subject>Spheroids, Cellular - drug effects</subject><subject>Spheroids, Cellular - metabolism</subject><subject>Spheroids, Cellular - pathology</subject><subject>STAT3 Transcription Factor - metabolism</subject><subject>Studies</subject><subject>Tissue Inhibitor of Metalloproteinases</subject><subject>Transcription</subject><subject>Tumor Stem Cell Assay</subject><subject>Tumorigenesis</subject><subject>Wound healing</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkTtPwzAUhS0EolAYWZElFpYUP-o4HquKl1SJgTJHtmMXV4kT7KQS_x5HDQxM917p09G55wBwg9GCFoI8NE1YEISLhVgSdAIuMBc4owgtT6edCMFn4DLGPUI5I0ycgxlFmBLGyQVwq7pz3vTOQ-c_nXJ9hF1oa2dNkL1rPZS-go3bTVdrYXuQwUkPtfTaBKhNXUd4cBLGoeuCiXHi3rerLYXR7bysnd9dgTMr62iupzkHH0-P2_VLtnl7fl2vNpmmOeqzgppcaKy40hoZYlTFKGZESS4pz5c0r5DIrdLK8pwyzC1jHFMtlZWSE1vQObg_6qY3vgYT-7JxcTQpvWmHWBIkcIEZEyihd__QfTuEZDdRGAmU0ipworIjpUMbYzC27IJrZPguMSrHDsrUQTl2UI4dJP52Uh1UY6o_-jd0-gOLR4MZ</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Zhao, Xuezhi</creator><creator>Guo, Xiaohan</creator><creator>Shen, Junhua</creator><creator>Hua, Dingchao</creator><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20181001</creationdate><title>Alpinetin inhibits proliferation and migration of ovarian cancer cells via suppression of STAT3 signaling</title><author>Zhao, Xuezhi ; Guo, Xiaohan ; Shen, Junhua ; Hua, Dingchao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-83e69c1b7bcc0e2ebd53152ba7a376436d096fbcbf763517f55713cabfaa72f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Anti-inflammatory agents</topic><topic>Antineoplastic Agents, Phytogenic - chemistry</topic><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>B-cell lymphoma</topic><topic>Bax protein</topic><topic>Bcl-2 protein</topic><topic>Biotechnology</topic><topic>c-Myc protein</topic><topic>Cancer therapies</topic><topic>Caspase</topic><topic>Caspase-3</topic><topic>Cell adhesion & migration</topic><topic>Cell cycle</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement - drug effects</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Chemotherapy</topic><topic>Colonies</topic><topic>Cyclin D1</topic><topic>Cyclin-dependent kinase 4</topic><topic>Cyclin-dependent kinases</topic><topic>Female</topic><topic>Flavanones - chemistry</topic><topic>Flavanones - pharmacology</topic><topic>Flow cytometry</topic><topic>G1 phase</topic><topic>G1 Phase - drug effects</topic><topic>Gelatinase A</topic><topic>Humans</topic><topic>Immunoglobulins</topic><topic>Inflammation</topic><topic>Kinases</topic><topic>Lung cancer</topic><topic>Lymphocytes B</topic><topic>Matrix Metalloproteinases - metabolism</topic><topic>Metastasis</topic><topic>Ovarian cancer</topic><topic>Ovarian Neoplasms - enzymology</topic><topic>Ovarian Neoplasms - metabolism</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Poly(ADP-ribose) polymerase</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>S Phase - drug effects</topic><topic>Signal transduction</topic><topic>Signal Transduction - drug effects</topic><topic>Spheroids, Cellular - drug effects</topic><topic>Spheroids, Cellular - metabolism</topic><topic>Spheroids, Cellular - pathology</topic><topic>STAT3 Transcription Factor - metabolism</topic><topic>Studies</topic><topic>Tissue Inhibitor of Metalloproteinases</topic><topic>Transcription</topic><topic>Tumor Stem Cell Assay</topic><topic>Tumorigenesis</topic><topic>Wound healing</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Xuezhi</creatorcontrib><creatorcontrib>Guo, Xiaohan</creatorcontrib><creatorcontrib>Shen, Junhua</creatorcontrib><creatorcontrib>Hua, Dingchao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Xuezhi</au><au>Guo, Xiaohan</au><au>Shen, Junhua</au><au>Hua, Dingchao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alpinetin inhibits proliferation and migration of ovarian cancer cells via suppression of STAT3 signaling</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2018-10-01</date><risdate>2018</risdate><volume>18</volume><issue>4</issue><spage>4030</spage><epage>4036</epage><pages>4030-4036</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>Natural bioactive components are increasingly being applied in cancer research. Alpinetin is a type of natural flavonoid primarily derived from Alpinia katsumadai Hayata, which exhibits anti‑bacterial and anti‑inflammatory properties. Therefore, it may possess anticancer potential and be employed therapeutically for different diseases. The aim of the present study was to investigate the anticancer effects of alpinetin on the SKOV3 ovarian cancer cell line. The effect of alpinetin treatment on SKOV3 cell proliferation, apoptosis, spheroid and colony formation were measured using Cell Counting kit‑8, cell apoptosis, 3D spheroid and colony formation assays, respectively. Analysis of the cell cycle was performed using flow cytometry. Western blot analysis was used to determine the protein expression levels of B‑cell lymphoma (Bcl)‑2, Bcl‑2‑associated X protein, cleaved caspase‑3, cleaved poly (ADP‑ribose) polymerase (PARP), cyclin D1, cyclin‑dependent kinase (CDK) 4, CDK6, signal transducer and activator of transcription (STAT) 3, phosphorylated (p)‑STAT3, c‑myc, survivin, tissue inhibitor of metalloproteinase (TIMP)‑1, TIMP‑2, matrix metalloproteinase (MMP)‑2 and MMP‑9. In addition, a wound healing assay was used to determine cancer cell migration. The results revealed alpinetin suppressed cell viability and induced apoptosis of SKOV3 cells in a dose‑ and time‑dependent manner, and cells were arrested in the G1 phase. Alpinetin treatment upregulated protein expression levels of Bax, cleaved caspase‑3 and PARP, and downregulated protein expression levels of Bcl‑2, cyclin D1, CDK4 and CDK6. Alpinetin also inhibited cell migration, through increased protein expression levels of TIMP‑1 and TIMP‑2, and decreased protein expression levels of MMP‑2 and MMP‑9. Alpinetin also significantly suppressed colony and spheroid formation by SKOV3 cells. In addition, the STAT3 pathway was suppressed as demonstrated by downregulation of p‑STAT3 and reduced expression of downstream factors, including c‑myc and survivin. Overall, these results indicated that alpinetin may have anticancer effects on human ovarian cancer by inhibiting the STAT3 signaling pathway.</abstract><cop>Greece</cop><pub>Spandidos Publications UK Ltd</pub><pmid>30132572</pmid><doi>10.3892/mmr.2018.9420</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Anti-inflammatory agents Antineoplastic Agents, Phytogenic - chemistry Antineoplastic Agents, Phytogenic - pharmacology Apoptosis Apoptosis - drug effects B-cell lymphoma Bax protein Bcl-2 protein Biotechnology c-Myc protein Cancer therapies Caspase Caspase-3 Cell adhesion & migration Cell cycle Cell Cycle Proteins - metabolism Cell growth Cell Line, Tumor Cell migration Cell Movement - drug effects Cell proliferation Cell Proliferation - drug effects Cell Survival - drug effects Chemotherapy Colonies Cyclin D1 Cyclin-dependent kinase 4 Cyclin-dependent kinases Female Flavanones - chemistry Flavanones - pharmacology Flow cytometry G1 phase G1 Phase - drug effects Gelatinase A Humans Immunoglobulins Inflammation Kinases Lung cancer Lymphocytes B Matrix Metalloproteinases - metabolism Metastasis Ovarian cancer Ovarian Neoplasms - enzymology Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology Poly(ADP-ribose) polymerase Protein expression Proteins S Phase - drug effects Signal transduction Signal Transduction - drug effects Spheroids, Cellular - drug effects Spheroids, Cellular - metabolism Spheroids, Cellular - pathology STAT3 Transcription Factor - metabolism Studies Tissue Inhibitor of Metalloproteinases Transcription Tumor Stem Cell Assay Tumorigenesis Wound healing |
| title | Alpinetin inhibits proliferation and migration of ovarian cancer cells via suppression of STAT3 signaling |
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