An off-on fluorescent probe for the detection of mitochondria-specific protein persulfidation
Protein persulfidation is a newly defined oxidative posttranslational modification and plays important roles in many biological processes. Detection of protein persulfidation in living systems is urgently needed to advance the study of H2S/H2Sn-based signalling and cellular redox regulation. Here, w...
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| Veröffentlicht in: | Organic & biomolecular chemistry 2018, Vol.16 (34), p.6350-6357 |
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| container_title | Organic & biomolecular chemistry |
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| creator | Meng, Wenqi Chen, Yongchun Feng, Yongwei Zhang, Hao Xu, Qingqiang Sun, Mingxue Shi, Wenwen Cen, Jinfeng Zhao, Jie Xiao, Kai |
| description | Protein persulfidation is a newly defined oxidative posttranslational modification and plays important roles in many biological processes. Detection of protein persulfidation in living systems is urgently needed to advance the study of H2S/H2Sn-based signalling and cellular redox regulation. Here, we developed a novel off-on fluorescent probe for the detection of persulfidation using a chemical sensor, HQO-SSH, in biological systems. HQO-SSH features fast reaction, good selectivity and high sensitivity. Due to the distinctive features of HQO-SSH, this probe was successfully applied to image protein persulfidation changes in pulmonary cells. We also demonstrated that the probe is suitable for imaging protein persulfidation in lung tissues. In addition, confocal imaging with this method revealed that sulfur mustard, a commonly used chemical warfare agent, decreased mitochondrial protein persulfidation in living lung cells and tissues. Due to these results, this probe holds great promise for exploring the role of protein persulfidation in a variety of pathophysiological conditions. |
| doi_str_mv | 10.1039/c8ob01608a |
| format | Article |
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Detection of protein persulfidation in living systems is urgently needed to advance the study of H2S/H2Sn-based signalling and cellular redox regulation. Here, we developed a novel off-on fluorescent probe for the detection of persulfidation using a chemical sensor, HQO-SSH, in biological systems. HQO-SSH features fast reaction, good selectivity and high sensitivity. Due to the distinctive features of HQO-SSH, this probe was successfully applied to image protein persulfidation changes in pulmonary cells. We also demonstrated that the probe is suitable for imaging protein persulfidation in lung tissues. In addition, confocal imaging with this method revealed that sulfur mustard, a commonly used chemical warfare agent, decreased mitochondrial protein persulfidation in living lung cells and tissues. Due to these results, this probe holds great promise for exploring the role of protein persulfidation in a variety of pathophysiological conditions.</description><identifier>ISSN: 1477-0520</identifier><identifier>EISSN: 1477-0539</identifier><identifier>DOI: 10.1039/c8ob01608a</identifier><identifier>PMID: 30132775</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>A549 Cells ; Animals ; Biological activity ; Chemical sensors ; Chemical warfare ; Fluorescent Dyes - metabolism ; Fluorescent indicators ; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - metabolism ; Humans ; Hydrogen sulfide ; Kinetics ; Lungs ; Male ; Mice ; Mitochondria ; Mitochondrial Proteins - metabolism ; Mustard gas ; Optical Imaging ; Organic chemistry ; Proteins ; Sulfides - metabolism ; Sulfur ; Tissues</subject><ispartof>Organic & biomolecular chemistry, 2018, Vol.16 (34), p.6350-6357</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-3aaa8d3df9c6b57813f0dec17598ada2a191dd86b8e24ea20c921f623e87b9b73</citedby><cites>FETCH-LOGICAL-c356t-3aaa8d3df9c6b57813f0dec17598ada2a191dd86b8e24ea20c921f623e87b9b73</cites><orcidid>0000-0002-2558-073X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30132775$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meng, Wenqi</creatorcontrib><creatorcontrib>Chen, Yongchun</creatorcontrib><creatorcontrib>Feng, Yongwei</creatorcontrib><creatorcontrib>Zhang, Hao</creatorcontrib><creatorcontrib>Xu, Qingqiang</creatorcontrib><creatorcontrib>Sun, Mingxue</creatorcontrib><creatorcontrib>Shi, Wenwen</creatorcontrib><creatorcontrib>Cen, Jinfeng</creatorcontrib><creatorcontrib>Zhao, Jie</creatorcontrib><creatorcontrib>Xiao, Kai</creatorcontrib><title>An off-on fluorescent probe for the detection of mitochondria-specific protein persulfidation</title><title>Organic & biomolecular chemistry</title><addtitle>Org Biomol Chem</addtitle><description>Protein persulfidation is a newly defined oxidative posttranslational modification and plays important roles in many biological processes. Detection of protein persulfidation in living systems is urgently needed to advance the study of H2S/H2Sn-based signalling and cellular redox regulation. Here, we developed a novel off-on fluorescent probe for the detection of persulfidation using a chemical sensor, HQO-SSH, in biological systems. HQO-SSH features fast reaction, good selectivity and high sensitivity. Due to the distinctive features of HQO-SSH, this probe was successfully applied to image protein persulfidation changes in pulmonary cells. We also demonstrated that the probe is suitable for imaging protein persulfidation in lung tissues. In addition, confocal imaging with this method revealed that sulfur mustard, a commonly used chemical warfare agent, decreased mitochondrial protein persulfidation in living lung cells and tissues. Due to these results, this probe holds great promise for exploring the role of protein persulfidation in a variety of pathophysiological conditions.</description><subject>A549 Cells</subject><subject>Animals</subject><subject>Biological activity</subject><subject>Chemical sensors</subject><subject>Chemical warfare</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Fluorescent indicators</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - metabolism</subject><subject>Humans</subject><subject>Hydrogen sulfide</subject><subject>Kinetics</subject><subject>Lungs</subject><subject>Male</subject><subject>Mice</subject><subject>Mitochondria</subject><subject>Mitochondrial Proteins - metabolism</subject><subject>Mustard gas</subject><subject>Optical Imaging</subject><subject>Organic chemistry</subject><subject>Proteins</subject><subject>Sulfides - metabolism</subject><subject>Sulfur</subject><subject>Tissues</subject><issn>1477-0520</issn><issn>1477-0539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0D1PwzAQBmALgSgUFn4AisSCkAL-SGJ7LBVfUqUuMKLIsc-qqyQOdjLw70lo6cB0Nzx3unsRuiL4nmAmH7TwFSYFFuoInZGM8xTnTB4feopn6DzGLcZE8iI7RTOGCaOc52foc9Em3trUt4mtBx8gamj7pAu-gsT6kPQbSAz0oHvnJ5o0rvd641sTnEpjB9pZp6eBHlybdBDiUFtn1OQv0IlVdYTLfZ2jj-en9-Vrulq_vC0Xq1SzvOhTppQShhkrdVHlXBBmsQFNeC6FMooqIokxoqgE0AwUxVpSYgvKQPBKVpzN0e1u73jG1wCxLxs3PlLXqgU_xJJiSQTJsSxGevOPbv0Q2vG6SeUyF5Rno7rbKR18jAFs2QXXqPBdElxOoZdLsX78DX0x4uv9yqFqwBzoX8rsB3BbfVI</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Meng, Wenqi</creator><creator>Chen, Yongchun</creator><creator>Feng, Yongwei</creator><creator>Zhang, Hao</creator><creator>Xu, Qingqiang</creator><creator>Sun, Mingxue</creator><creator>Shi, Wenwen</creator><creator>Cen, Jinfeng</creator><creator>Zhao, Jie</creator><creator>Xiao, Kai</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2558-073X</orcidid></search><sort><creationdate>2018</creationdate><title>An off-on fluorescent probe for the detection of mitochondria-specific protein persulfidation</title><author>Meng, Wenqi ; Chen, Yongchun ; Feng, Yongwei ; Zhang, Hao ; Xu, Qingqiang ; Sun, Mingxue ; Shi, Wenwen ; Cen, Jinfeng ; Zhao, Jie ; Xiao, Kai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-3aaa8d3df9c6b57813f0dec17598ada2a191dd86b8e24ea20c921f623e87b9b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>A549 Cells</topic><topic>Animals</topic><topic>Biological activity</topic><topic>Chemical sensors</topic><topic>Chemical warfare</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Fluorescent indicators</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - metabolism</topic><topic>Humans</topic><topic>Hydrogen sulfide</topic><topic>Kinetics</topic><topic>Lungs</topic><topic>Male</topic><topic>Mice</topic><topic>Mitochondria</topic><topic>Mitochondrial Proteins - metabolism</topic><topic>Mustard gas</topic><topic>Optical Imaging</topic><topic>Organic chemistry</topic><topic>Proteins</topic><topic>Sulfides - metabolism</topic><topic>Sulfur</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meng, Wenqi</creatorcontrib><creatorcontrib>Chen, Yongchun</creatorcontrib><creatorcontrib>Feng, Yongwei</creatorcontrib><creatorcontrib>Zhang, Hao</creatorcontrib><creatorcontrib>Xu, Qingqiang</creatorcontrib><creatorcontrib>Sun, Mingxue</creatorcontrib><creatorcontrib>Shi, Wenwen</creatorcontrib><creatorcontrib>Cen, Jinfeng</creatorcontrib><creatorcontrib>Zhao, Jie</creatorcontrib><creatorcontrib>Xiao, Kai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Organic & biomolecular chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meng, Wenqi</au><au>Chen, Yongchun</au><au>Feng, Yongwei</au><au>Zhang, Hao</au><au>Xu, Qingqiang</au><au>Sun, Mingxue</au><au>Shi, Wenwen</au><au>Cen, Jinfeng</au><au>Zhao, Jie</au><au>Xiao, Kai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An off-on fluorescent probe for the detection of mitochondria-specific protein persulfidation</atitle><jtitle>Organic & biomolecular chemistry</jtitle><addtitle>Org Biomol Chem</addtitle><date>2018</date><risdate>2018</risdate><volume>16</volume><issue>34</issue><spage>6350</spage><epage>6357</epage><pages>6350-6357</pages><issn>1477-0520</issn><eissn>1477-0539</eissn><abstract>Protein persulfidation is a newly defined oxidative posttranslational modification and plays important roles in many biological processes. Detection of protein persulfidation in living systems is urgently needed to advance the study of H2S/H2Sn-based signalling and cellular redox regulation. Here, we developed a novel off-on fluorescent probe for the detection of persulfidation using a chemical sensor, HQO-SSH, in biological systems. HQO-SSH features fast reaction, good selectivity and high sensitivity. Due to the distinctive features of HQO-SSH, this probe was successfully applied to image protein persulfidation changes in pulmonary cells. We also demonstrated that the probe is suitable for imaging protein persulfidation in lung tissues. In addition, confocal imaging with this method revealed that sulfur mustard, a commonly used chemical warfare agent, decreased mitochondrial protein persulfidation in living lung cells and tissues. Due to these results, this probe holds great promise for exploring the role of protein persulfidation in a variety of pathophysiological conditions.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>30132775</pmid><doi>10.1039/c8ob01608a</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-2558-073X</orcidid></addata></record> |
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| subjects | A549 Cells Animals Biological activity Chemical sensors Chemical warfare Fluorescent Dyes - metabolism Fluorescent indicators Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - metabolism Humans Hydrogen sulfide Kinetics Lungs Male Mice Mitochondria Mitochondrial Proteins - metabolism Mustard gas Optical Imaging Organic chemistry Proteins Sulfides - metabolism Sulfur Tissues |
| title | An off-on fluorescent probe for the detection of mitochondria-specific protein persulfidation |
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