Real-Time Monitoring of Fluorescence in Situ Hybridization Kinetics

We present a novel method for real-time monitoring and kinetic analysis of fluorescence in situ hybridization (FISH). We implement the method using a vertical microfluidic probe containing a microstructure designed for rapid switching between probe solution and nonfluorescent imaging buffer. The FIS...

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Veröffentlicht in:	Analytical chemistry (Washington) 2018-10, Vol.90 (19), p.11470-11477
Hauptverfasser:	Ostromohov, Nadya, Huber, Deborah, Bercovici, Moran, Kaigala, Govind V
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Buffers Chemistry Dextran Dextran sulfate Fluorescence Fluorescence in situ hybridization Ionic strength Kinetics Microfluidics Microstructure Probes Real time Signal monitoring
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