Regenerated Hair Cells Become Functional during Continuous Administration of Kanamycin
The compound action potential (CAP) was used to assess the functional status of regenerated hair cells in the chick cochlea during prolonged administration of kanamycin (KM). Immediately after 10 days of KM treatment, the CAP thresholds were elevated by 6–54 dB above those from age-matched control a...
Gespeichert in:
| Veröffentlicht in: | Audiology & neurotology 1998-07, Vol.3 (4), p.229-239 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 239 |
|---|---|
| container_issue | 4 |
| container_start_page | 229 |
| container_title | Audiology & neurotology |
| container_volume | 3 |
| creator | Trautwein, Patricia G. Hashino, Eri Salvi, Richard J. |
| description | The compound action potential (CAP) was used to assess the functional status of regenerated hair cells in the chick cochlea during prolonged administration of kanamycin (KM). Immediately after 10 days of KM treatment, the CAP thresholds were elevated by 6–54 dB above those from age-matched control animals. The frequencies with the greatest threshold shifts (>1 kHz) corresponded to the hair cell lesion in the basal 40% of the basilar papilla. After 20 days of KM, the CAP thresholds at 3 and 4 kHz were significantly lower than those after 10 days of KM treatment, but virtually the same as those after 10 days of KM plus 10 days of recovery. Similarly, the CAP amplitudes at frequencies higher than 1.5 kHz were significantly greater in animals that received KM for 20 days than in animals that received KM for 10 days. The threshold as well as amplitude improvement between 10 days and 20 days of KM treatment was associated with the morphological maturation of the regenerated hair cells in the basal 25% of the cochlea. In addition, the rapid functional recovery seen at high frequencies coincided with the base-to-apex gradient of morphological recovery in the basilar papilla. These results suggest that the process of hair cell maturation is not suppressed by the presence of aminoglycosides in the extracellular environment. |
| doi_str_mv | 10.1159/000013795 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_20909448</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79965676</sourcerecordid><originalsourceid>FETCH-LOGICAL-c414t-93ff25f89e508283cd993a9261c4cc59c54f7702ebe116dd9f965d5da54867253</originalsourceid><addsrcrecordid>eNqF0UtLJDEQAOCwKL7Wg2cRwiCCh3bz7HSO46irOLCw6F6bmMcQ7U6PSffBf2-caUZYBHNJoL6qSlIAHGF0gTGXv1BemArJf4A9zAgupEBoa3VGBUUU7YL9lJ6z4pyzHbAjS8Y45Xvg31-7sMFG1VsDb5WPcGabJsFLq7vWwpsh6N53QTXQDNGHBZx1ofdh6IYEp6b1wac-J2cCOwfvVVDtm_bhJ9h2qkn2cNwPwOPN9cPstpj_-X03m84LzTDrC0mdI9xV0nJUkYpqIyVVkpRYM6251Jw5IRCxTxbj0hjpZMkNN4qzqhSE0wNwtq67jN3rYFNftz7p_AIVbL5iLWROKEX5LSRIIslYleHkP_jcDTF_QDYEVTgTkdH5GunYpRStq5fRtyq-1RjVHxOpNxPJ9mQsODy11mzkOIIcPx3jKmnVuKiC9mnDCJGsEh_seM1eVFzY-NlwbDL5Mjp9vFqBemkcfQfxe6Vs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>220814837</pqid></control><display><type>article</type><title>Regenerated Hair Cells Become Functional during Continuous Administration of Kanamycin</title><source>MEDLINE</source><source>Karger Journals Complete</source><creator>Trautwein, Patricia G. ; Hashino, Eri ; Salvi, Richard J.</creator><creatorcontrib>Trautwein, Patricia G. ; Hashino, Eri ; Salvi, Richard J.</creatorcontrib><description>The compound action potential (CAP) was used to assess the functional status of regenerated hair cells in the chick cochlea during prolonged administration of kanamycin (KM). Immediately after 10 days of KM treatment, the CAP thresholds were elevated by 6–54 dB above those from age-matched control animals. The frequencies with the greatest threshold shifts (>1 kHz) corresponded to the hair cell lesion in the basal 40% of the basilar papilla. After 20 days of KM, the CAP thresholds at 3 and 4 kHz were significantly lower than those after 10 days of KM treatment, but virtually the same as those after 10 days of KM plus 10 days of recovery. Similarly, the CAP amplitudes at frequencies higher than 1.5 kHz were significantly greater in animals that received KM for 20 days than in animals that received KM for 10 days. The threshold as well as amplitude improvement between 10 days and 20 days of KM treatment was associated with the morphological maturation of the regenerated hair cells in the basal 25% of the cochlea. In addition, the rapid functional recovery seen at high frequencies coincided with the base-to-apex gradient of morphological recovery in the basilar papilla. These results suggest that the process of hair cell maturation is not suppressed by the presence of aminoglycosides in the extracellular environment.</description><identifier>ISSN: 1420-3030</identifier><identifier>EISSN: 1421-9700</identifier><identifier>DOI: 10.1159/000013795</identifier><identifier>PMID: 9644535</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Action Potentials - drug effects ; Age Factors ; Animals ; Anti-Bacterial Agents - pharmacology ; Antineoplastic agents ; Auditory Threshold - drug effects ; Biological and medical sciences ; Chemotherapy ; Chickens ; Dose-Response Relationship, Drug ; Drug Administration Schedule ; Hair Cells, Auditory - drug effects ; Kanamycin - pharmacology ; Medical sciences ; Nerve Regeneration - drug effects ; Original Paper ; Pharmacology. Drug treatments</subject><ispartof>Audiology & neurotology, 1998-07, Vol.3 (4), p.229-239</ispartof><rights>1998 S. Karger AG, Basel</rights><rights>1998 INIST-CNRS</rights><rights>Copyright (c) 1998 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-93ff25f89e508283cd993a9261c4cc59c54f7702ebe116dd9f965d5da54867253</citedby><cites>FETCH-LOGICAL-c414t-93ff25f89e508283cd993a9261c4cc59c54f7702ebe116dd9f965d5da54867253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2429,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2294875$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9644535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Trautwein, Patricia G.</creatorcontrib><creatorcontrib>Hashino, Eri</creatorcontrib><creatorcontrib>Salvi, Richard J.</creatorcontrib><title>Regenerated Hair Cells Become Functional during Continuous Administration of Kanamycin</title><title>Audiology & neurotology</title><addtitle>Audiol Neurotol</addtitle><description>The compound action potential (CAP) was used to assess the functional status of regenerated hair cells in the chick cochlea during prolonged administration of kanamycin (KM). Immediately after 10 days of KM treatment, the CAP thresholds were elevated by 6–54 dB above those from age-matched control animals. The frequencies with the greatest threshold shifts (>1 kHz) corresponded to the hair cell lesion in the basal 40% of the basilar papilla. After 20 days of KM, the CAP thresholds at 3 and 4 kHz were significantly lower than those after 10 days of KM treatment, but virtually the same as those after 10 days of KM plus 10 days of recovery. Similarly, the CAP amplitudes at frequencies higher than 1.5 kHz were significantly greater in animals that received KM for 20 days than in animals that received KM for 10 days. The threshold as well as amplitude improvement between 10 days and 20 days of KM treatment was associated with the morphological maturation of the regenerated hair cells in the basal 25% of the cochlea. In addition, the rapid functional recovery seen at high frequencies coincided with the base-to-apex gradient of morphological recovery in the basilar papilla. These results suggest that the process of hair cell maturation is not suppressed by the presence of aminoglycosides in the extracellular environment.</description><subject>Action Potentials - drug effects</subject><subject>Age Factors</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antineoplastic agents</subject><subject>Auditory Threshold - drug effects</subject><subject>Biological and medical sciences</subject><subject>Chemotherapy</subject><subject>Chickens</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Administration Schedule</subject><subject>Hair Cells, Auditory - drug effects</subject><subject>Kanamycin - pharmacology</subject><subject>Medical sciences</subject><subject>Nerve Regeneration - drug effects</subject><subject>Original Paper</subject><subject>Pharmacology. Drug treatments</subject><issn>1420-3030</issn><issn>1421-9700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0UtLJDEQAOCwKL7Wg2cRwiCCh3bz7HSO46irOLCw6F6bmMcQ7U6PSffBf2-caUZYBHNJoL6qSlIAHGF0gTGXv1BemArJf4A9zAgupEBoa3VGBUUU7YL9lJ6z4pyzHbAjS8Y45Xvg31-7sMFG1VsDb5WPcGabJsFLq7vWwpsh6N53QTXQDNGHBZx1ofdh6IYEp6b1wac-J2cCOwfvVVDtm_bhJ9h2qkn2cNwPwOPN9cPstpj_-X03m84LzTDrC0mdI9xV0nJUkYpqIyVVkpRYM6251Jw5IRCxTxbj0hjpZMkNN4qzqhSE0wNwtq67jN3rYFNftz7p_AIVbL5iLWROKEX5LSRIIslYleHkP_jcDTF_QDYEVTgTkdH5GunYpRStq5fRtyq-1RjVHxOpNxPJ9mQsODy11mzkOIIcPx3jKmnVuKiC9mnDCJGsEh_seM1eVFzY-NlwbDL5Mjp9vFqBemkcfQfxe6Vs</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>Trautwein, Patricia G.</creator><creator>Hashino, Eri</creator><creator>Salvi, Richard J.</creator><general>Karger</general><general>S. Karger AG</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19980701</creationdate><title>Regenerated Hair Cells Become Functional during Continuous Administration of Kanamycin</title><author>Trautwein, Patricia G. ; Hashino, Eri ; Salvi, Richard J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-93ff25f89e508283cd993a9261c4cc59c54f7702ebe116dd9f965d5da54867253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Action Potentials - drug effects</topic><topic>Age Factors</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antineoplastic agents</topic><topic>Auditory Threshold - drug effects</topic><topic>Biological and medical sciences</topic><topic>Chemotherapy</topic><topic>Chickens</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Administration Schedule</topic><topic>Hair Cells, Auditory - drug effects</topic><topic>Kanamycin - pharmacology</topic><topic>Medical sciences</topic><topic>Nerve Regeneration - drug effects</topic><topic>Original Paper</topic><topic>Pharmacology. Drug treatments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trautwein, Patricia G.</creatorcontrib><creatorcontrib>Hashino, Eri</creatorcontrib><creatorcontrib>Salvi, Richard J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Audiology & neurotology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Trautwein, Patricia G.</au><au>Hashino, Eri</au><au>Salvi, Richard J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regenerated Hair Cells Become Functional during Continuous Administration of Kanamycin</atitle><jtitle>Audiology & neurotology</jtitle><addtitle>Audiol Neurotol</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>3</volume><issue>4</issue><spage>229</spage><epage>239</epage><pages>229-239</pages><issn>1420-3030</issn><eissn>1421-9700</eissn><abstract>The compound action potential (CAP) was used to assess the functional status of regenerated hair cells in the chick cochlea during prolonged administration of kanamycin (KM). Immediately after 10 days of KM treatment, the CAP thresholds were elevated by 6–54 dB above those from age-matched control animals. The frequencies with the greatest threshold shifts (>1 kHz) corresponded to the hair cell lesion in the basal 40% of the basilar papilla. After 20 days of KM, the CAP thresholds at 3 and 4 kHz were significantly lower than those after 10 days of KM treatment, but virtually the same as those after 10 days of KM plus 10 days of recovery. Similarly, the CAP amplitudes at frequencies higher than 1.5 kHz were significantly greater in animals that received KM for 20 days than in animals that received KM for 10 days. The threshold as well as amplitude improvement between 10 days and 20 days of KM treatment was associated with the morphological maturation of the regenerated hair cells in the basal 25% of the cochlea. In addition, the rapid functional recovery seen at high frequencies coincided with the base-to-apex gradient of morphological recovery in the basilar papilla. These results suggest that the process of hair cell maturation is not suppressed by the presence of aminoglycosides in the extracellular environment.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>9644535</pmid><doi>10.1159/000013795</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1420-3030 |
| ispartof | Audiology & neurotology, 1998-07, Vol.3 (4), p.229-239 |
| issn | 1420-3030 1421-9700 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_20909448 |
| source | MEDLINE; Karger Journals Complete |
| subjects | Action Potentials - drug effects Age Factors Animals Anti-Bacterial Agents - pharmacology Antineoplastic agents Auditory Threshold - drug effects Biological and medical sciences Chemotherapy Chickens Dose-Response Relationship, Drug Drug Administration Schedule Hair Cells, Auditory - drug effects Kanamycin - pharmacology Medical sciences Nerve Regeneration - drug effects Original Paper Pharmacology. Drug treatments |
| title | Regenerated Hair Cells Become Functional during Continuous Administration of Kanamycin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T22%3A28%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Regenerated%20Hair%20Cells%20Become%20Functional%20during%20Continuous%20Administration%20of%20Kanamycin&rft.jtitle=Audiology%20&%20neurotology&rft.au=Trautwein,%20Patricia%20G.&rft.date=1998-07-01&rft.volume=3&rft.issue=4&rft.spage=229&rft.epage=239&rft.pages=229-239&rft.issn=1420-3030&rft.eissn=1421-9700&rft_id=info:doi/10.1159/000013795&rft_dat=%3Cproquest_pubme%3E79965676%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=220814837&rft_id=info:pmid/9644535&rfr_iscdi=true |