Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR
To determine the presence of toxin genes in 22 Clostridium perfringens isolated from turkey meat samples by molecular typing. For this purpose, alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) toxin genes were analysed by multiplex PCR. All 22 turkey meat Cl. pe...
Gespeichert in:
| Veröffentlicht in: | Letters in applied microbiology 2008-07, Vol.47 (1), p.31-34 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 34 |
|---|---|
| container_issue | 1 |
| container_start_page | 31 |
| container_title | Letters in applied microbiology |
| container_volume | 47 |
| creator | Erol, I Goncuoglu, M Ayaz, N.D Bilir Ormanci, F.S Hildebrandt, G |
| description | To determine the presence of toxin genes in 22 Clostridium perfringens isolated from turkey meat samples by molecular typing. For this purpose, alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) toxin genes were analysed by multiplex PCR. All 22 turkey meat Cl. perfringens isolates were found to carry the cpa, gene but in none of the isolates cpb, etx, iap or cpe genes were detected. Results showed that all isolates represented type A and were cpe negative. Our results indicate that Cl. perfringens type A is the most common type in turkey meat. Also multiplex PCR is effective and rapid method for typing of Cl. perfringens. It is the first study about molecular typing of Cl. perfringens using multiplex PCR in turkey meat samples in Turkey. |
| doi_str_mv | 10.1111/j.1472-765X.2008.02379.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_20908900</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20908900</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5189-4b17aea9c9d28f1c6098058d704e64d2919c11c0377381165a1d2eff48b119e3</originalsourceid><addsrcrecordid>eNqNkEGP1CAUx4nRuOPqV1Auemt9j9IWDh42E3VNZqPR1XgjDIUNI51WaOPMt5fuTNarXB6B3_89-BFCEUrM6-2uRN6yom3qnyUDECWwqpXl4RFZPVw8JitgTVMIVvML8iylHWQSmXxKLlDUNWdNtSI_boZgzRx0pNNx9Ps7Oji6DkOaou_83NPRRhfzud0n6tMQ9GQ76uLQ02mOv-yR9lZPdJvrHCY_BnugX9Zfn5MnTodkX5zrJbn98P52fV1sPn_8tL7aFKZGIQu-xVZbLY3smHBoGpACatG1wG3DOyZRGkQDVdtWArGpNXbMOsfFFlHa6pK8ObUd4_B7tmlSvU_GhqD3dpiTYiBBSIAMihNo4pBStE6N0fc6HhWCWpSqnVrMqcWcWpSqe6XqkKMvzzPmbW-7f8Gzwwy8PgM6GR1c1Hvj0wPHgHPBpczcuxP3xwd7_O8HqM3VzbLL-VenvNOD0ncxz_j-jQFWkH9ZA2-qv7DFm2g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20908900</pqid></control><display><type>article</type><title>Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Erol, I ; Goncuoglu, M ; Ayaz, N.D ; Bilir Ormanci, F.S ; Hildebrandt, G</creator><creatorcontrib>Erol, I ; Goncuoglu, M ; Ayaz, N.D ; Bilir Ormanci, F.S ; Hildebrandt, G</creatorcontrib><description>To determine the presence of toxin genes in 22 Clostridium perfringens isolated from turkey meat samples by molecular typing. For this purpose, alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) toxin genes were analysed by multiplex PCR. All 22 turkey meat Cl. perfringens isolates were found to carry the cpa, gene but in none of the isolates cpb, etx, iap or cpe genes were detected. Results showed that all isolates represented type A and were cpe negative. Our results indicate that Cl. perfringens type A is the most common type in turkey meat. Also multiplex PCR is effective and rapid method for typing of Cl. perfringens. It is the first study about molecular typing of Cl. perfringens using multiplex PCR in turkey meat samples in Turkey.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/j.1472-765X.2008.02379.x</identifier><identifier>PMID: 18554263</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Animals ; Bacterial Typing Techniques ; Biological and medical sciences ; Clostridium Infections - microbiology ; Clostridium Infections - veterinary ; Clostridium perfringens ; Clostridium perfringens - classification ; Clostridium perfringens - genetics ; Clostridium perfringens - isolation & purification ; DNA, Bacterial - analysis ; Enterotoxins - genetics ; Food Contamination ; Fundamental and applied biological sciences. Psychology ; Meat - microbiology ; Microbiology ; Polymerase Chain Reaction ; toxin genes ; turkey meat ; Turkeys - microbiology</subject><ispartof>Letters in applied microbiology, 2008-07, Vol.47 (1), p.31-34</ispartof><rights>2008 The Authors. Journal compilation © 2008 The Society for Applied Microbiology</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5189-4b17aea9c9d28f1c6098058d704e64d2919c11c0377381165a1d2eff48b119e3</citedby><cites>FETCH-LOGICAL-c5189-4b17aea9c9d28f1c6098058d704e64d2919c11c0377381165a1d2eff48b119e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1472-765X.2008.02379.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1472-765X.2008.02379.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20448499$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18554263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Erol, I</creatorcontrib><creatorcontrib>Goncuoglu, M</creatorcontrib><creatorcontrib>Ayaz, N.D</creatorcontrib><creatorcontrib>Bilir Ormanci, F.S</creatorcontrib><creatorcontrib>Hildebrandt, G</creatorcontrib><title>Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>To determine the presence of toxin genes in 22 Clostridium perfringens isolated from turkey meat samples by molecular typing. For this purpose, alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) toxin genes were analysed by multiplex PCR. All 22 turkey meat Cl. perfringens isolates were found to carry the cpa, gene but in none of the isolates cpb, etx, iap or cpe genes were detected. Results showed that all isolates represented type A and were cpe negative. Our results indicate that Cl. perfringens type A is the most common type in turkey meat. Also multiplex PCR is effective and rapid method for typing of Cl. perfringens. It is the first study about molecular typing of Cl. perfringens using multiplex PCR in turkey meat samples in Turkey.</description><subject>Animals</subject><subject>Bacterial Typing Techniques</subject><subject>Biological and medical sciences</subject><subject>Clostridium Infections - microbiology</subject><subject>Clostridium Infections - veterinary</subject><subject>Clostridium perfringens</subject><subject>Clostridium perfringens - classification</subject><subject>Clostridium perfringens - genetics</subject><subject>Clostridium perfringens - isolation & purification</subject><subject>DNA, Bacterial - analysis</subject><subject>Enterotoxins - genetics</subject><subject>Food Contamination</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Meat - microbiology</subject><subject>Microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>toxin genes</subject><subject>turkey meat</subject><subject>Turkeys - microbiology</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEGP1CAUx4nRuOPqV1Auemt9j9IWDh42E3VNZqPR1XgjDIUNI51WaOPMt5fuTNarXB6B3_89-BFCEUrM6-2uRN6yom3qnyUDECWwqpXl4RFZPVw8JitgTVMIVvML8iylHWQSmXxKLlDUNWdNtSI_boZgzRx0pNNx9Ps7Oji6DkOaou_83NPRRhfzud0n6tMQ9GQ76uLQ02mOv-yR9lZPdJvrHCY_BnugX9Zfn5MnTodkX5zrJbn98P52fV1sPn_8tL7aFKZGIQu-xVZbLY3smHBoGpACatG1wG3DOyZRGkQDVdtWArGpNXbMOsfFFlHa6pK8ObUd4_B7tmlSvU_GhqD3dpiTYiBBSIAMihNo4pBStE6N0fc6HhWCWpSqnVrMqcWcWpSqe6XqkKMvzzPmbW-7f8Gzwwy8PgM6GR1c1Hvj0wPHgHPBpczcuxP3xwd7_O8HqM3VzbLL-VenvNOD0ncxz_j-jQFWkH9ZA2-qv7DFm2g</recordid><startdate>200807</startdate><enddate>200807</enddate><creator>Erol, I</creator><creator>Goncuoglu, M</creator><creator>Ayaz, N.D</creator><creator>Bilir Ormanci, F.S</creator><creator>Hildebrandt, G</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>200807</creationdate><title>Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR</title><author>Erol, I ; Goncuoglu, M ; Ayaz, N.D ; Bilir Ormanci, F.S ; Hildebrandt, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5189-4b17aea9c9d28f1c6098058d704e64d2919c11c0377381165a1d2eff48b119e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Bacterial Typing Techniques</topic><topic>Biological and medical sciences</topic><topic>Clostridium Infections - microbiology</topic><topic>Clostridium Infections - veterinary</topic><topic>Clostridium perfringens</topic><topic>Clostridium perfringens - classification</topic><topic>Clostridium perfringens - genetics</topic><topic>Clostridium perfringens - isolation & purification</topic><topic>DNA, Bacterial - analysis</topic><topic>Enterotoxins - genetics</topic><topic>Food Contamination</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Meat - microbiology</topic><topic>Microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>toxin genes</topic><topic>turkey meat</topic><topic>Turkeys - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Erol, I</creatorcontrib><creatorcontrib>Goncuoglu, M</creatorcontrib><creatorcontrib>Ayaz, N.D</creatorcontrib><creatorcontrib>Bilir Ormanci, F.S</creatorcontrib><creatorcontrib>Hildebrandt, G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Erol, I</au><au>Goncuoglu, M</au><au>Ayaz, N.D</au><au>Bilir Ormanci, F.S</au><au>Hildebrandt, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2008-07</date><risdate>2008</risdate><volume>47</volume><issue>1</issue><spage>31</spage><epage>34</epage><pages>31-34</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><abstract>To determine the presence of toxin genes in 22 Clostridium perfringens isolated from turkey meat samples by molecular typing. For this purpose, alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) toxin genes were analysed by multiplex PCR. All 22 turkey meat Cl. perfringens isolates were found to carry the cpa, gene but in none of the isolates cpb, etx, iap or cpe genes were detected. Results showed that all isolates represented type A and were cpe negative. Our results indicate that Cl. perfringens type A is the most common type in turkey meat. Also multiplex PCR is effective and rapid method for typing of Cl. perfringens. It is the first study about molecular typing of Cl. perfringens using multiplex PCR in turkey meat samples in Turkey.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18554263</pmid><doi>10.1111/j.1472-765X.2008.02379.x</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0266-8254 |
| ispartof | Letters in applied microbiology, 2008-07, Vol.47 (1), p.31-34 |
| issn | 0266-8254 1472-765X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_20908900 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Bacterial Typing Techniques Biological and medical sciences Clostridium Infections - microbiology Clostridium Infections - veterinary Clostridium perfringens Clostridium perfringens - classification Clostridium perfringens - genetics Clostridium perfringens - isolation & purification DNA, Bacterial - analysis Enterotoxins - genetics Food Contamination Fundamental and applied biological sciences. Psychology Meat - microbiology Microbiology Polymerase Chain Reaction toxin genes turkey meat Turkeys - microbiology |
| title | Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T00%3A21%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20typing%20of%20Clostridium%20perfringens%20isolated%20from%20turkey%20meat%20by%20multiplex%20PCR&rft.jtitle=Letters%20in%20applied%20microbiology&rft.au=Erol,%20I&rft.date=2008-07&rft.volume=47&rft.issue=1&rft.spage=31&rft.epage=34&rft.pages=31-34&rft.issn=0266-8254&rft.eissn=1472-765X&rft.coden=LAMIE7&rft_id=info:doi/10.1111/j.1472-765X.2008.02379.x&rft_dat=%3Cproquest_cross%3E20908900%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20908900&rft_id=info:pmid/18554263&rfr_iscdi=true |