Effects of bioactive peptide, valyl-prolyl-proline (VPP), and Lactobacillus helveticus fermented milk containing VPP on bone loss in ovariectomized rats
Background: Valyl-prolyl-proline (VPP), a bioactive peptide formed during the fermentation with Lactobacillus helveticus LBK-16H (L. helveticus), has been shown to increase bone formation in vitro. The aim of the study was to determine whether VPP and L. helveticus fermented milk prevent bone loss i...
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| Veröffentlicht in: | Annals of nutrition and metabolism 2007-01, Vol.51 (1), p.65-74 |
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| creator | Narva, Mirkka Rissanen, Jukka Halleen, Jussi Vapaatalo, Heikki Väänänen, Kalervo Korpela, Riitta |
| description | Background: Valyl-prolyl-proline (VPP), a bioactive peptide formed during the fermentation with Lactobacillus helveticus LBK-16H (L. helveticus), has been shown to increase bone formation in vitro. The aim of the study was to determine whether VPP and L. helveticus fermented milk prevent bone loss in ovariectomized (OVX) rats. Methods: During the 12-week intervention study, the OVX rats received VPP in water or L. helveticus fermented milk, containing VPP. Sham-operated rats receiving water acted as controls. The trabecular and cortical bone mineral density were determined by peripheral quantitative computed tomography before the operation and at 4 and 12 weeks. The mechanical testing and ash weight analysis as well as the static and dynamic histomorphometrical parameters were assessed at the end of the intervention. Results: VPP given in water showed no clear effect on bone loss. L. helveticus fermented milk prevented bone loss by decreasing bone turnover and increasing the bone mineral density. Ovariectomy caused a 57% loss in the trabecular bone, which was attenuated by 16% in the L. helveticus group. Conclusions: VPP peptide did not prevent ovariectomy-induced bone loss, which could be due to the poor bioavailability of VPP from water solution. L. helveticus fermented milk prevented bone loss, whether this is due to the VPP peptide cannot be concluded. |
| doi_str_mv | 10.1159/000100823 |
| format | Article |
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The aim of the study was to determine whether VPP and L. helveticus fermented milk prevent bone loss in ovariectomized (OVX) rats. Methods: During the 12-week intervention study, the OVX rats received VPP in water or L. helveticus fermented milk, containing VPP. Sham-operated rats receiving water acted as controls. The trabecular and cortical bone mineral density were determined by peripheral quantitative computed tomography before the operation and at 4 and 12 weeks. The mechanical testing and ash weight analysis as well as the static and dynamic histomorphometrical parameters were assessed at the end of the intervention. Results: VPP given in water showed no clear effect on bone loss. L. helveticus fermented milk prevented bone loss by decreasing bone turnover and increasing the bone mineral density. Ovariectomy caused a 57% loss in the trabecular bone, which was attenuated by 16% in the L. helveticus group. Conclusions: VPP peptide did not prevent ovariectomy-induced bone loss, which could be due to the poor bioavailability of VPP from water solution. L. helveticus fermented milk prevented bone loss, whether this is due to the VPP peptide cannot be concluded.</description><identifier>ISSN: 0250-6807</identifier><identifier>EISSN: 1421-9697</identifier><identifier>DOI: 10.1159/000100823</identifier><identifier>PMID: 17356257</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>animal models ; Animals ; Bioavailability ; Bone density ; Bone Density - drug effects ; Bone mineral density ; bone mineralization ; bone resorption ; Computed tomography ; Cultured Milk Products ; cultured milk starters ; Female ; Fermentation ; fermented milk ; lactic acid bacteria ; Lactobacillus helveticus ; Milk ; Nutrition ; Oligopeptides - pharmacology ; Original Paper ; Osteoporosis - etiology ; Osteoporosis - prevention & control ; ovariectomy ; Ovariectomy - adverse effects ; Peptides ; Probiotics ; Probiotics - pharmacology ; proline ; Rats ; Receiving waters ; Rodents</subject><ispartof>Annals of nutrition and metabolism, 2007-01, Vol.51 (1), p.65-74</ispartof><rights>2007 S. Karger AG</rights><rights>2007 S. Karger AG, Basel</rights><rights>Copyright 2007 S. Karger AG, Basel.</rights><rights>Copyright (c) 2007 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-1278f28fa018ff4a70389e3356e30752b08cf621ece3162bcb8d8c05490789ad3</citedby><cites>FETCH-LOGICAL-c408t-1278f28fa018ff4a70389e3356e30752b08cf621ece3162bcb8d8c05490789ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/48507643$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/48507643$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2429,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17356257$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Narva, Mirkka</creatorcontrib><creatorcontrib>Rissanen, Jukka</creatorcontrib><creatorcontrib>Halleen, Jussi</creatorcontrib><creatorcontrib>Vapaatalo, Heikki</creatorcontrib><creatorcontrib>Väänänen, Kalervo</creatorcontrib><creatorcontrib>Korpela, Riitta</creatorcontrib><title>Effects of bioactive peptide, valyl-prolyl-proline (VPP), and Lactobacillus helveticus fermented milk containing VPP on bone loss in ovariectomized rats</title><title>Annals of nutrition and metabolism</title><addtitle>Ann Nutr Metab</addtitle><description>Background: Valyl-prolyl-proline (VPP), a bioactive peptide formed during the fermentation with Lactobacillus helveticus LBK-16H (L. helveticus), has been shown to increase bone formation in vitro. The aim of the study was to determine whether VPP and L. helveticus fermented milk prevent bone loss in ovariectomized (OVX) rats. Methods: During the 12-week intervention study, the OVX rats received VPP in water or L. helveticus fermented milk, containing VPP. Sham-operated rats receiving water acted as controls. The trabecular and cortical bone mineral density were determined by peripheral quantitative computed tomography before the operation and at 4 and 12 weeks. The mechanical testing and ash weight analysis as well as the static and dynamic histomorphometrical parameters were assessed at the end of the intervention. Results: VPP given in water showed no clear effect on bone loss. L. helveticus fermented milk prevented bone loss by decreasing bone turnover and increasing the bone mineral density. Ovariectomy caused a 57% loss in the trabecular bone, which was attenuated by 16% in the L. helveticus group. Conclusions: VPP peptide did not prevent ovariectomy-induced bone loss, which could be due to the poor bioavailability of VPP from water solution. L. helveticus fermented milk prevented bone loss, whether this is due to the VPP peptide cannot be concluded.</description><subject>animal models</subject><subject>Animals</subject><subject>Bioavailability</subject><subject>Bone density</subject><subject>Bone Density - drug effects</subject><subject>Bone mineral density</subject><subject>bone mineralization</subject><subject>bone resorption</subject><subject>Computed tomography</subject><subject>Cultured Milk Products</subject><subject>cultured milk starters</subject><subject>Female</subject><subject>Fermentation</subject><subject>fermented milk</subject><subject>lactic acid bacteria</subject><subject>Lactobacillus helveticus</subject><subject>Milk</subject><subject>Nutrition</subject><subject>Oligopeptides - pharmacology</subject><subject>Original Paper</subject><subject>Osteoporosis - etiology</subject><subject>Osteoporosis - prevention & control</subject><subject>ovariectomy</subject><subject>Ovariectomy - adverse effects</subject><subject>Peptides</subject><subject>Probiotics</subject><subject>Probiotics - pharmacology</subject><subject>proline</subject><subject>Rats</subject><subject>Receiving waters</subject><subject>Rodents</subject><issn>0250-6807</issn><issn>1421-9697</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUtv1DAUhS0EokNhwZ6H1QWiUgPXr8RZoqo8pJGoBGUbOc714GkSD3YyUvkl_bl4NANFbFhdS-c7R773EPKUwRvGVP0WABiA5uIeWTDJWVGXdXWfLIArKEoN1RF5lNI6U1xL9ZAcsUqokqtqQW4vnEM7JRocbX0wdvJbpBvcTL7DM7o1_U1fbGL4PfyI9PW3y8vTM2rGji6zIbTG-r6fE_2O_RYnb_PTYRxwnLCjg--vqQ3jZPzoxxXNZhpG2oac1IeUqB9p2Jro8zfC4H9mSzRTekweONMnfHKYx-Tq_cXX84_F8vOHT-fvloWVoKeC8Uo7rp0Bpp2TpgKhaxR5PRRQKd6Ctq7kDC0KVvLWtrrTFpSsodK16cQxebXPzdv9mDFNzeCTxb43I4Y5NRVIBlrV_wU51KBLKTN48g-4DnMc8xINF5zlxvgu7XQP2ZhvENE1m-gHE28aBs2u1OZPqZl9cQic2wG7O_LQYgae7YFrE1cY_4o6-J_v5XWawp0qtYKqlDv95V53JjRmFX1qrr5wYALyjfKHlfgFdpe4Hw</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Narva, Mirkka</creator><creator>Rissanen, Jukka</creator><creator>Halleen, Jussi</creator><creator>Vapaatalo, Heikki</creator><creator>Väänänen, Kalervo</creator><creator>Korpela, Riitta</creator><general>S. 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drug effects</topic><topic>Bone mineral density</topic><topic>bone mineralization</topic><topic>bone resorption</topic><topic>Computed tomography</topic><topic>Cultured Milk Products</topic><topic>cultured milk starters</topic><topic>Female</topic><topic>Fermentation</topic><topic>fermented milk</topic><topic>lactic acid bacteria</topic><topic>Lactobacillus helveticus</topic><topic>Milk</topic><topic>Nutrition</topic><topic>Oligopeptides - pharmacology</topic><topic>Original Paper</topic><topic>Osteoporosis - etiology</topic><topic>Osteoporosis - prevention & control</topic><topic>ovariectomy</topic><topic>Ovariectomy - adverse effects</topic><topic>Peptides</topic><topic>Probiotics</topic><topic>Probiotics - pharmacology</topic><topic>proline</topic><topic>Rats</topic><topic>Receiving waters</topic><topic>Rodents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Narva, Mirkka</creatorcontrib><creatorcontrib>Rissanen, Jukka</creatorcontrib><creatorcontrib>Halleen, Jussi</creatorcontrib><creatorcontrib>Vapaatalo, Heikki</creatorcontrib><creatorcontrib>Väänänen, Kalervo</creatorcontrib><creatorcontrib>Korpela, Riitta</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>eLibrary</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Agricultural Science Database</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of nutrition and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Narva, Mirkka</au><au>Rissanen, Jukka</au><au>Halleen, Jussi</au><au>Vapaatalo, Heikki</au><au>Väänänen, Kalervo</au><au>Korpela, Riitta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of bioactive peptide, valyl-prolyl-proline (VPP), and Lactobacillus helveticus fermented milk containing VPP on bone loss in ovariectomized rats</atitle><jtitle>Annals of nutrition and metabolism</jtitle><addtitle>Ann Nutr Metab</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>51</volume><issue>1</issue><spage>65</spage><epage>74</epage><pages>65-74</pages><issn>0250-6807</issn><eissn>1421-9697</eissn><abstract>Background: Valyl-prolyl-proline (VPP), a bioactive peptide formed during the fermentation with Lactobacillus helveticus LBK-16H (L. helveticus), has been shown to increase bone formation in vitro. The aim of the study was to determine whether VPP and L. helveticus fermented milk prevent bone loss in ovariectomized (OVX) rats. Methods: During the 12-week intervention study, the OVX rats received VPP in water or L. helveticus fermented milk, containing VPP. Sham-operated rats receiving water acted as controls. The trabecular and cortical bone mineral density were determined by peripheral quantitative computed tomography before the operation and at 4 and 12 weeks. The mechanical testing and ash weight analysis as well as the static and dynamic histomorphometrical parameters were assessed at the end of the intervention. Results: VPP given in water showed no clear effect on bone loss. L. helveticus fermented milk prevented bone loss by decreasing bone turnover and increasing the bone mineral density. Ovariectomy caused a 57% loss in the trabecular bone, which was attenuated by 16% in the L. helveticus group. Conclusions: VPP peptide did not prevent ovariectomy-induced bone loss, which could be due to the poor bioavailability of VPP from water solution. L. helveticus fermented milk prevented bone loss, whether this is due to the VPP peptide cannot be concluded.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>17356257</pmid><doi>10.1159/000100823</doi><tpages>10</tpages></addata></record> |
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| subjects | animal models Animals Bioavailability Bone density Bone Density - drug effects Bone mineral density bone mineralization bone resorption Computed tomography Cultured Milk Products cultured milk starters Female Fermentation fermented milk lactic acid bacteria Lactobacillus helveticus Milk Nutrition Oligopeptides - pharmacology Original Paper Osteoporosis - etiology Osteoporosis - prevention & control ovariectomy Ovariectomy - adverse effects Peptides Probiotics Probiotics - pharmacology proline Rats Receiving waters Rodents |
| title | Effects of bioactive peptide, valyl-prolyl-proline (VPP), and Lactobacillus helveticus fermented milk containing VPP on bone loss in ovariectomized rats |
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