Genomewide Oligonucleotide Microarray Analysis on Placentae of Pre-Eclamptic Pregnancies
Objective: Human placentae from normal and pre-eclamptic pregnancies were evaluated for possible changes in gene expression by microarray analysis to uncover new clues for the research of the etiology of pre-eclampsia. Methods: Placentae from five normal pregnancies and five pregnancies complicated...
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| Veröffentlicht in: | Gynecologic and obstetric investigation 2006-08, Vol.62 (2), p.108-114 |
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| creator | Zhou, Rongrong Zhu, Qianyong Wang, Yahui Ren, Yonghong Zhang, Liang Zhou, Yuxiang |
| description | Objective: Human placentae from normal and pre-eclamptic pregnancies were evaluated for possible changes in gene expression by microarray analysis to uncover new clues for the research of the etiology of pre-eclampsia. Methods: Placentae from five normal pregnancies and five pregnancies complicated by pre-eclampsia were collected. mRNA levels of five pre-eclamptic placentae were examined using genome-wide 70-mer oligonucleotide microarrays (CapitalBio, Beijing, China) in comparison with the pooled control consisting of total RNA from five normotensive placentae. Results: Ninety-six genes were found consistently down- or up-regulated in at least four pre-eclamptic samples. Most of them were related to an imbalance of reactive oxygen metabolites in placenta, abnormal trophoblast invasion, disorders of lipoprotein metabolism and signal transduction, or some that have been reported to have close correlation to the pathology of pre-eclampsia. The microarray data were also confirmed by the measurement of real-time PCR. Conclusion: DNA microarray is a high throughput and time-saving method to monitor altered gene expression. The results could provide interesting clues to the etiology of pre-eclampsia and lead to further studies in a more targeted fashion. |
| doi_str_mv | 10.1159/000092857 |
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Methods: Placentae from five normal pregnancies and five pregnancies complicated by pre-eclampsia were collected. mRNA levels of five pre-eclamptic placentae were examined using genome-wide 70-mer oligonucleotide microarrays (CapitalBio, Beijing, China) in comparison with the pooled control consisting of total RNA from five normotensive placentae. Results: Ninety-six genes were found consistently down- or up-regulated in at least four pre-eclamptic samples. Most of them were related to an imbalance of reactive oxygen metabolites in placenta, abnormal trophoblast invasion, disorders of lipoprotein metabolism and signal transduction, or some that have been reported to have close correlation to the pathology of pre-eclampsia. The microarray data were also confirmed by the measurement of real-time PCR. Conclusion: DNA microarray is a high throughput and time-saving method to monitor altered gene expression. The results could provide interesting clues to the etiology of pre-eclampsia and lead to further studies in a more targeted fashion.</description><identifier>ISSN: 0378-7346</identifier><identifier>EISSN: 1423-002X</identifier><identifier>DOI: 10.1159/000092857</identifier><identifier>PMID: 16651850</identifier><identifier>CODEN: GOBIDS</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Adult ; Biological and medical sciences ; Case-Control Studies ; Diseases of mother, fetus and pregnancy ; Female ; Gene Expression ; Gene Expression Profiling ; Gynecology. Andrology. Obstetrics ; Humans ; Lipoproteins - metabolism ; Medical sciences ; Oligonucleotide Array Sequence Analysis ; Original Article ; Placenta - metabolism ; Polymerase Chain Reaction - methods ; Pre-Eclampsia - genetics ; Pre-Eclampsia - metabolism ; Pregnancy ; Pregnancy. Fetus. Placenta ; Reactive Oxygen Species - metabolism ; RNA, Messenger - analysis ; Signal Transduction ; Trophoblasts - metabolism</subject><ispartof>Gynecologic and obstetric investigation, 2006-08, Vol.62 (2), p.108-114</ispartof><rights>2006 S. Karger AG, Basel</rights><rights>2006 INIST-CNRS</rights><rights>Copyright (c) 2006 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c306t-d36f924245ca82fa115de5a46045986b821827693df9e8d6d82897d118b97b133</citedby><cites>FETCH-LOGICAL-c306t-d36f924245ca82fa115de5a46045986b821827693df9e8d6d82897d118b97b133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2429,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18022041$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16651850$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Rongrong</creatorcontrib><creatorcontrib>Zhu, Qianyong</creatorcontrib><creatorcontrib>Wang, Yahui</creatorcontrib><creatorcontrib>Ren, Yonghong</creatorcontrib><creatorcontrib>Zhang, Liang</creatorcontrib><creatorcontrib>Zhou, Yuxiang</creatorcontrib><title>Genomewide Oligonucleotide Microarray Analysis on Placentae of Pre-Eclamptic Pregnancies</title><title>Gynecologic and obstetric investigation</title><addtitle>Gynecol Obstet Invest</addtitle><description>Objective: Human placentae from normal and pre-eclamptic pregnancies were evaluated for possible changes in gene expression by microarray analysis to uncover new clues for the research of the etiology of pre-eclampsia. Methods: Placentae from five normal pregnancies and five pregnancies complicated by pre-eclampsia were collected. mRNA levels of five pre-eclamptic placentae were examined using genome-wide 70-mer oligonucleotide microarrays (CapitalBio, Beijing, China) in comparison with the pooled control consisting of total RNA from five normotensive placentae. Results: Ninety-six genes were found consistently down- or up-regulated in at least four pre-eclamptic samples. Most of them were related to an imbalance of reactive oxygen metabolites in placenta, abnormal trophoblast invasion, disorders of lipoprotein metabolism and signal transduction, or some that have been reported to have close correlation to the pathology of pre-eclampsia. The microarray data were also confirmed by the measurement of real-time PCR. Conclusion: DNA microarray is a high throughput and time-saving method to monitor altered gene expression. The results could provide interesting clues to the etiology of pre-eclampsia and lead to further studies in a more targeted fashion.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Case-Control Studies</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Lipoproteins - metabolism</subject><subject>Medical sciences</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Original Article</subject><subject>Placenta - metabolism</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Pre-Eclampsia - genetics</subject><subject>Pre-Eclampsia - metabolism</subject><subject>Pregnancy</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>Signal Transduction</subject><subject>Trophoblasts - metabolism</subject><issn>0378-7346</issn><issn>1423-002X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqF0c9LHDEUB_AglrpqD56FMhQs9DD25cfkx1FktYUVPVjwNmQzb5bRzGRNZij735tlF4Vemkt48OEl7_sIOaNwSWllfkI-hulKHZAZFYyXAOzpkMyAK10qLuQROU7pGSBjLT6TIyplRXUFM_J0i0Po8W_XYHHvu1UYJucxjNv6rnMx2BjtprgarN-kLhVhKB68dTiMFovQFg8Ry7nztl-PndtWq8EOrsN0Sj611if8sr9PyJ-b-eP1r3Jxf_v7-mpROg5yLBsuW8MEE5WzmrU2z9NgZYUEkf8ql5pRzZQ0vGkN6kY2mmmjGkr10qgl5fyEfN_1XcfwOmEa675LDr23A4Yp1VIrrjj8HzIwIKRhGX77Bz6HKeYAsmFcSVppyOjHDuWIUorY1uvY9TZuagr1din1-1Ky_bpvOC17bD7kfgsZXOyBTc76Nm4jTB9OA2MgaHbnO_di4wrjO9g98wbDXpqc</recordid><startdate>200608</startdate><enddate>200608</enddate><creator>Zhou, Rongrong</creator><creator>Zhu, Qianyong</creator><creator>Wang, Yahui</creator><creator>Ren, Yonghong</creator><creator>Zhang, Liang</creator><creator>Zhou, Yuxiang</creator><general>Karger</general><general>S. Karger AG</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7TM</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200608</creationdate><title>Genomewide Oligonucleotide Microarray Analysis on Placentae of Pre-Eclamptic Pregnancies</title><author>Zhou, Rongrong ; Zhu, Qianyong ; Wang, Yahui ; Ren, Yonghong ; Zhang, Liang ; Zhou, Yuxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-d36f924245ca82fa115de5a46045986b821827693df9e8d6d82897d118b97b133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Case-Control Studies</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Lipoproteins - metabolism</topic><topic>Medical sciences</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Original Article</topic><topic>Placenta - metabolism</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Pre-Eclampsia - genetics</topic><topic>Pre-Eclampsia - metabolism</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>Signal Transduction</topic><topic>Trophoblasts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Rongrong</creatorcontrib><creatorcontrib>Zhu, Qianyong</creatorcontrib><creatorcontrib>Wang, Yahui</creatorcontrib><creatorcontrib>Ren, Yonghong</creatorcontrib><creatorcontrib>Zhang, Liang</creatorcontrib><creatorcontrib>Zhou, Yuxiang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Nucleic Acids Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecologic and obstetric investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Rongrong</au><au>Zhu, Qianyong</au><au>Wang, Yahui</au><au>Ren, Yonghong</au><au>Zhang, Liang</au><au>Zhou, Yuxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomewide Oligonucleotide Microarray Analysis on Placentae of Pre-Eclamptic Pregnancies</atitle><jtitle>Gynecologic and obstetric investigation</jtitle><addtitle>Gynecol Obstet Invest</addtitle><date>2006-08</date><risdate>2006</risdate><volume>62</volume><issue>2</issue><spage>108</spage><epage>114</epage><pages>108-114</pages><issn>0378-7346</issn><eissn>1423-002X</eissn><coden>GOBIDS</coden><abstract>Objective: Human placentae from normal and pre-eclamptic pregnancies were evaluated for possible changes in gene expression by microarray analysis to uncover new clues for the research of the etiology of pre-eclampsia. Methods: Placentae from five normal pregnancies and five pregnancies complicated by pre-eclampsia were collected. mRNA levels of five pre-eclamptic placentae were examined using genome-wide 70-mer oligonucleotide microarrays (CapitalBio, Beijing, China) in comparison with the pooled control consisting of total RNA from five normotensive placentae. Results: Ninety-six genes were found consistently down- or up-regulated in at least four pre-eclamptic samples. Most of them were related to an imbalance of reactive oxygen metabolites in placenta, abnormal trophoblast invasion, disorders of lipoprotein metabolism and signal transduction, or some that have been reported to have close correlation to the pathology of pre-eclampsia. The microarray data were also confirmed by the measurement of real-time PCR. Conclusion: DNA microarray is a high throughput and time-saving method to monitor altered gene expression. The results could provide interesting clues to the etiology of pre-eclampsia and lead to further studies in a more targeted fashion.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>16651850</pmid><doi>10.1159/000092857</doi><tpages>7</tpages></addata></record> |
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| ispartof | Gynecologic and obstetric investigation, 2006-08, Vol.62 (2), p.108-114 |
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| subjects | Adult Biological and medical sciences Case-Control Studies Diseases of mother, fetus and pregnancy Female Gene Expression Gene Expression Profiling Gynecology. Andrology. Obstetrics Humans Lipoproteins - metabolism Medical sciences Oligonucleotide Array Sequence Analysis Original Article Placenta - metabolism Polymerase Chain Reaction - methods Pre-Eclampsia - genetics Pre-Eclampsia - metabolism Pregnancy Pregnancy. Fetus. Placenta Reactive Oxygen Species - metabolism RNA, Messenger - analysis Signal Transduction Trophoblasts - metabolism |
| title | Genomewide Oligonucleotide Microarray Analysis on Placentae of Pre-Eclamptic Pregnancies |
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