Characterisation of proteins in epidermal mucus of discus fish ( Symphysodon spp.) during parental phase
We conducted a series of experiments to investigate protein content and properties of epidermal mucus from discus fish ( Symphysodon spp.), a cichlid species demonstrating parental feeding of larvae with mucus secretion. Observation on the ontogenic development of larval biting behavior showed that...
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| Veröffentlicht in: | Aquaculture 2005-09, Vol.249 (1), p.469-476 |
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| creator | Chong, Kenny Sock Ying, Tham Foo, John Toong Jin, Lam Chong, Alexander |
| description | We conducted a series of experiments to investigate protein content and properties of epidermal mucus from discus fish (
Symphysodon spp.), a cichlid species demonstrating parental feeding of larvae with mucus secretion. Observation on the ontogenic development of larval biting behavior showed that feeding rate on parental mucus increased until 15 days of free-swimming period, followed by a decreasing trend, presumably coinciding with the onset of full capacity for exogenous feeding. Analysis of total protein content showed a higher amount in parental mucus (0.73
±
0.03 mg per ml) as compared to juvenile mucus (0.63
±
0.01 mg per ml). Electrophoretic pattern also showed similar proteins with differences in levels of expression between parental and juvenile fish mucus. Mass spectrometry analysis revealed the increased expression of a discus type II epidermal keratin in parental mucus. Free amino acid analysis also showed the presence of several essential amino acids such as lysine and phenylalanine in mucus of parental fish. |
| doi_str_mv | 10.1016/j.aquaculture.2005.02.045 |
| format | Article |
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Symphysodon spp.), a cichlid species demonstrating parental feeding of larvae with mucus secretion. Observation on the ontogenic development of larval biting behavior showed that feeding rate on parental mucus increased until 15 days of free-swimming period, followed by a decreasing trend, presumably coinciding with the onset of full capacity for exogenous feeding. Analysis of total protein content showed a higher amount in parental mucus (0.73
±
0.03 mg per ml) as compared to juvenile mucus (0.63
±
0.01 mg per ml). Electrophoretic pattern also showed similar proteins with differences in levels of expression between parental and juvenile fish mucus. Mass spectrometry analysis revealed the increased expression of a discus type II epidermal keratin in parental mucus. Free amino acid analysis also showed the presence of several essential amino acids such as lysine and phenylalanine in mucus of parental fish.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2005.02.045</identifier><identifier>CODEN: AQCLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Agnatha. Pisces ; Animal aquaculture ; Animal productions ; Animal reproduction ; Aquaculture ; Biological and medical sciences ; Cichlidae ; Freshwater ; Fundamental and applied biological sciences. Psychology ; General aspects ; Mucous membrane ; Nutrition ; Proteins ; Symphysodon ; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><ispartof>Aquaculture, 2005-09, Vol.249 (1), p.469-476</ispartof><rights>2005 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Elsevier Sequoia S.A. Sep 12, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-b8547ec454dd723b7859e6b55b0314e9830f65881a84f29bf688f86995ea18413</citedby><cites>FETCH-LOGICAL-c440t-b8547ec454dd723b7859e6b55b0314e9830f65881a84f29bf688f86995ea18413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0044848605001304$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17059383$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Chong, Kenny</creatorcontrib><creatorcontrib>Sock Ying, Tham</creatorcontrib><creatorcontrib>Foo, John</creatorcontrib><creatorcontrib>Toong Jin, Lam</creatorcontrib><creatorcontrib>Chong, Alexander</creatorcontrib><title>Characterisation of proteins in epidermal mucus of discus fish ( Symphysodon spp.) during parental phase</title><title>Aquaculture</title><description>We conducted a series of experiments to investigate protein content and properties of epidermal mucus from discus fish (
Symphysodon spp.), a cichlid species demonstrating parental feeding of larvae with mucus secretion. Observation on the ontogenic development of larval biting behavior showed that feeding rate on parental mucus increased until 15 days of free-swimming period, followed by a decreasing trend, presumably coinciding with the onset of full capacity for exogenous feeding. Analysis of total protein content showed a higher amount in parental mucus (0.73
±
0.03 mg per ml) as compared to juvenile mucus (0.63
±
0.01 mg per ml). Electrophoretic pattern also showed similar proteins with differences in levels of expression between parental and juvenile fish mucus. Mass spectrometry analysis revealed the increased expression of a discus type II epidermal keratin in parental mucus. Free amino acid analysis also showed the presence of several essential amino acids such as lysine and phenylalanine in mucus of parental fish.</description><subject>Agnatha. Pisces</subject><subject>Animal aquaculture</subject><subject>Animal productions</subject><subject>Animal reproduction</subject><subject>Aquaculture</subject><subject>Biological and medical sciences</subject><subject>Cichlidae</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Mucous membrane</subject><subject>Nutrition</subject><subject>Proteins</subject><subject>Symphysodon</subject><subject>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><issn>0044-8486</issn><issn>1873-5622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkU2r1DAUhoMoOF79D1VQdNF60iZpspTBL7jgQl2HND2xGdq0N2mE-fdmmAuKG13lQJ73PR8vIc8pNBSoeHtqzF02Ns97jti0ALyBtgHGH5ADlX1Xc9G2D8kBgLFaMikekycpnQBACE4PZDpOJhq7Y_TJ7H4N1eqqLa47-pAqHyrc_IhxMXO1ZJvT5Xv06VI5n6bqdfX1vGzTOa1j0aZta95UY44-_Kg2EzHsRbhNJuFT8siZOeGz-_eGfP_w_tvxU3375ePn47vb2jIGez1Iznq0jLNx7Ntu6CVXKAbOB-goQyU7cIJLSY1krlWDE1I6KZTiaKhktLshr66-ZYm7jGnXSxkX59kEXHPSLSjoVM_-CdJecKWkKuCLv8DTmmMoSxQz1gNQzgukrpCNa0oRnd6iX0w8awr6kpQ-6T-S0pekNLS6JFW0L-8bmGTN7KIJ1qffBj1w1cmucMcrh-V-Pz1GnazHYHH0Ee2ux9X_R7df8fyvUw</recordid><startdate>20050912</startdate><enddate>20050912</enddate><creator>Chong, Kenny</creator><creator>Sock Ying, Tham</creator><creator>Foo, John</creator><creator>Toong Jin, Lam</creator><creator>Chong, Alexander</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier Sequoia S.A</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20050912</creationdate><title>Characterisation of proteins in epidermal mucus of discus fish ( Symphysodon spp.) during parental phase</title><author>Chong, Kenny ; Sock Ying, Tham ; Foo, John ; Toong Jin, Lam ; Chong, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-b8547ec454dd723b7859e6b55b0314e9830f65881a84f29bf688f86995ea18413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Agnatha. Pisces</topic><topic>Animal aquaculture</topic><topic>Animal productions</topic><topic>Animal reproduction</topic><topic>Aquaculture</topic><topic>Biological and medical sciences</topic><topic>Cichlidae</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Mucous membrane</topic><topic>Nutrition</topic><topic>Proteins</topic><topic>Symphysodon</topic><topic>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chong, Kenny</creatorcontrib><creatorcontrib>Sock Ying, Tham</creatorcontrib><creatorcontrib>Foo, John</creatorcontrib><creatorcontrib>Toong Jin, Lam</creatorcontrib><creatorcontrib>Chong, Alexander</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Aquaculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chong, Kenny</au><au>Sock Ying, Tham</au><au>Foo, John</au><au>Toong Jin, Lam</au><au>Chong, Alexander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation of proteins in epidermal mucus of discus fish ( Symphysodon spp.) during parental phase</atitle><jtitle>Aquaculture</jtitle><date>2005-09-12</date><risdate>2005</risdate><volume>249</volume><issue>1</issue><spage>469</spage><epage>476</epage><pages>469-476</pages><issn>0044-8486</issn><eissn>1873-5622</eissn><coden>AQCLAL</coden><abstract>We conducted a series of experiments to investigate protein content and properties of epidermal mucus from discus fish (
Symphysodon spp.), a cichlid species demonstrating parental feeding of larvae with mucus secretion. Observation on the ontogenic development of larval biting behavior showed that feeding rate on parental mucus increased until 15 days of free-swimming period, followed by a decreasing trend, presumably coinciding with the onset of full capacity for exogenous feeding. Analysis of total protein content showed a higher amount in parental mucus (0.73
±
0.03 mg per ml) as compared to juvenile mucus (0.63
±
0.01 mg per ml). Electrophoretic pattern also showed similar proteins with differences in levels of expression between parental and juvenile fish mucus. Mass spectrometry analysis revealed the increased expression of a discus type II epidermal keratin in parental mucus. Free amino acid analysis also showed the presence of several essential amino acids such as lysine and phenylalanine in mucus of parental fish.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.aquaculture.2005.02.045</doi><tpages>8</tpages></addata></record> |
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| subjects | Agnatha. Pisces Animal aquaculture Animal productions Animal reproduction Aquaculture Biological and medical sciences Cichlidae Freshwater Fundamental and applied biological sciences. Psychology General aspects Mucous membrane Nutrition Proteins Symphysodon Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution |
| title | Characterisation of proteins in epidermal mucus of discus fish ( Symphysodon spp.) during parental phase |
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