Development of Nanoscale Oil Bodies for Targeted Treatment of Lung Cancer
Lung cancer is the most widespread disease and is frequently associated with a high level of epidermal growth factor receptor (EGFR). This study was thus conducted to provide a proof-of-concept approach for targeted therapy of lung cancer by development of nanoscale oil bodies (NOBs). This was carri...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2018-09, Vol.66 (36), p.9438-9445 |
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| container_end_page | 9445 |
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| container_issue | 36 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Chiang, Chung-Jen Lin, Li-Jen Wu, Chia-Pei Chen, Chih-Jung Chao, Yun-Peng |
| description | Lung cancer is the most widespread disease and is frequently associated with a high level of epidermal growth factor receptor (EGFR). This study was thus conducted to provide a proof-of-concept approach for targeted therapy of lung cancer by development of nanoscale oil bodies (NOBs). This was carried out by fusion of anti-EGFR affibody (ZEGFR2) with oleosin (Ole), a structure protein of plant seed oils. The fusion protein (Ole-ZEGFR2) was produced in Escherichia coli. NOBs were spontaneously assembled from plant oil, phospholipids, and Ole-ZEGFR2. Consequently, Ole-ZEGFR2-based NOBs were selectively internalized by EGFR-positive lung cancer cells with an efficiency exceeding 90%. Furthermore, the hydrophobic anticancer drug, camptothecin (CPT), was encapsulated into Ole-ZEGFR2-based NOBs. The administration of the CPT formulation based on NOBs resulted in a strong antitumor activity both in vitro and in vivo. |
| doi_str_mv | 10.1021/acs.jafc.8b02972 |
| format | Article |
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This study was thus conducted to provide a proof-of-concept approach for targeted therapy of lung cancer by development of nanoscale oil bodies (NOBs). This was carried out by fusion of anti-EGFR affibody (ZEGFR2) with oleosin (Ole), a structure protein of plant seed oils. The fusion protein (Ole-ZEGFR2) was produced in Escherichia coli. NOBs were spontaneously assembled from plant oil, phospholipids, and Ole-ZEGFR2. Consequently, Ole-ZEGFR2-based NOBs were selectively internalized by EGFR-positive lung cancer cells with an efficiency exceeding 90%. Furthermore, the hydrophobic anticancer drug, camptothecin (CPT), was encapsulated into Ole-ZEGFR2-based NOBs. The administration of the CPT formulation based on NOBs resulted in a strong antitumor activity both in vitro and in vivo.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.8b02972</identifier><identifier>PMID: 30122032</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Antineoplastic Agents, Phytogenic - administration & dosage ; Antineoplastic Agents, Phytogenic - chemistry ; Camptothecin - administration & dosage ; Camptothecin - chemistry ; Drug Compounding ; Female ; Humans ; Lung Neoplasms - drug therapy ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Mice ; Mice, Inbred BALB C ; Molecular Targeted Therapy ; Nanostructures - administration & dosage ; Nanostructures - chemistry ; Plant Oils - chemistry ; Receptor, Epidermal Growth Factor - antagonists & inhibitors ; Receptor, Epidermal Growth Factor - genetics ; Receptor, Epidermal Growth Factor - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2018-09, Vol.66 (36), p.9438-9445</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a336t-a5ba3ba8ef879e1b0f1a421f2db8039719e13defa829572dc36121481fd3d26f3</citedby><cites>FETCH-LOGICAL-a336t-a5ba3ba8ef879e1b0f1a421f2db8039719e13defa829572dc36121481fd3d26f3</cites><orcidid>0000-0001-7997-0752</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.8b02972$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.8b02972$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30122032$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chiang, Chung-Jen</creatorcontrib><creatorcontrib>Lin, Li-Jen</creatorcontrib><creatorcontrib>Wu, Chia-Pei</creatorcontrib><creatorcontrib>Chen, Chih-Jung</creatorcontrib><creatorcontrib>Chao, Yun-Peng</creatorcontrib><title>Development of Nanoscale Oil Bodies for Targeted Treatment of Lung Cancer</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Lung cancer is the most widespread disease and is frequently associated with a high level of epidermal growth factor receptor (EGFR). This study was thus conducted to provide a proof-of-concept approach for targeted therapy of lung cancer by development of nanoscale oil bodies (NOBs). This was carried out by fusion of anti-EGFR affibody (ZEGFR2) with oleosin (Ole), a structure protein of plant seed oils. The fusion protein (Ole-ZEGFR2) was produced in Escherichia coli. NOBs were spontaneously assembled from plant oil, phospholipids, and Ole-ZEGFR2. Consequently, Ole-ZEGFR2-based NOBs were selectively internalized by EGFR-positive lung cancer cells with an efficiency exceeding 90%. Furthermore, the hydrophobic anticancer drug, camptothecin (CPT), was encapsulated into Ole-ZEGFR2-based NOBs. The administration of the CPT formulation based on NOBs resulted in a strong antitumor activity both in vitro and in vivo.</description><subject>Animals</subject><subject>Antineoplastic Agents, Phytogenic - administration & dosage</subject><subject>Antineoplastic Agents, Phytogenic - chemistry</subject><subject>Camptothecin - administration & dosage</subject><subject>Camptothecin - chemistry</subject><subject>Drug Compounding</subject><subject>Female</subject><subject>Humans</subject><subject>Lung Neoplasms - drug therapy</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Targeted Therapy</subject><subject>Nanostructures - administration & dosage</subject><subject>Nanostructures - chemistry</subject><subject>Plant Oils - chemistry</subject><subject>Receptor, Epidermal Growth Factor - antagonists & inhibitors</subject><subject>Receptor, Epidermal Growth Factor - genetics</subject><subject>Receptor, Epidermal Growth Factor - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kL9PwzAQhS0EoqWwMyGPDKTc2U3ijFB-VaroUmbLSc5VqjQudoLEf09KWzamk56-96T7GLtGGCMIvDdFGK-NLcYqB5Gl4oQNMRYQxYjqlA2hZyIVJzhgFyGsAUDFKZyzgQQUAqQYstkTfVHtthtqWu4sfzeNC4WpiS-qmj-6sqLArfN8afyKWir50pNpj_i8a1Z8apqC_CU7s6YOdHW4I_bx8rycvkXzxets-jCPjJRJG5k4NzI3iqxKM8IcLJqJQCvKXIHMUuxDWZI1SmRxKspCJihwotCWshSJlSN2u9_devfZUWj1pgoF1bVpyHVBC8hAolIx9Cjs0cK7EDxZvfXVxvhvjaB3AnUvUO8E6oPAvnJzWO_yDZV_haOxHrjbA79V1_mmf_b_vR-HnXsu</recordid><startdate>20180912</startdate><enddate>20180912</enddate><creator>Chiang, Chung-Jen</creator><creator>Lin, Li-Jen</creator><creator>Wu, Chia-Pei</creator><creator>Chen, Chih-Jung</creator><creator>Chao, Yun-Peng</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7997-0752</orcidid></search><sort><creationdate>20180912</creationdate><title>Development of Nanoscale Oil Bodies for Targeted Treatment of Lung Cancer</title><author>Chiang, Chung-Jen ; Lin, Li-Jen ; Wu, Chia-Pei ; Chen, Chih-Jung ; Chao, Yun-Peng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-a5ba3ba8ef879e1b0f1a421f2db8039719e13defa829572dc36121481fd3d26f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Antineoplastic Agents, Phytogenic - administration & dosage</topic><topic>Antineoplastic Agents, Phytogenic - chemistry</topic><topic>Camptothecin - administration & dosage</topic><topic>Camptothecin - chemistry</topic><topic>Drug Compounding</topic><topic>Female</topic><topic>Humans</topic><topic>Lung Neoplasms - drug therapy</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Targeted Therapy</topic><topic>Nanostructures - administration & dosage</topic><topic>Nanostructures - chemistry</topic><topic>Plant Oils - chemistry</topic><topic>Receptor, Epidermal Growth Factor - antagonists & inhibitors</topic><topic>Receptor, Epidermal Growth Factor - genetics</topic><topic>Receptor, Epidermal Growth Factor - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiang, Chung-Jen</creatorcontrib><creatorcontrib>Lin, Li-Jen</creatorcontrib><creatorcontrib>Wu, Chia-Pei</creatorcontrib><creatorcontrib>Chen, Chih-Jung</creatorcontrib><creatorcontrib>Chao, Yun-Peng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiang, Chung-Jen</au><au>Lin, Li-Jen</au><au>Wu, Chia-Pei</au><au>Chen, Chih-Jung</au><au>Chao, Yun-Peng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of Nanoscale Oil Bodies for Targeted Treatment of Lung Cancer</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2018-09-12</date><risdate>2018</risdate><volume>66</volume><issue>36</issue><spage>9438</spage><epage>9445</epage><pages>9438-9445</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Lung cancer is the most widespread disease and is frequently associated with a high level of epidermal growth factor receptor (EGFR). This study was thus conducted to provide a proof-of-concept approach for targeted therapy of lung cancer by development of nanoscale oil bodies (NOBs). This was carried out by fusion of anti-EGFR affibody (ZEGFR2) with oleosin (Ole), a structure protein of plant seed oils. The fusion protein (Ole-ZEGFR2) was produced in Escherichia coli. NOBs were spontaneously assembled from plant oil, phospholipids, and Ole-ZEGFR2. Consequently, Ole-ZEGFR2-based NOBs were selectively internalized by EGFR-positive lung cancer cells with an efficiency exceeding 90%. 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| ispartof | Journal of agricultural and food chemistry, 2018-09, Vol.66 (36), p.9438-9445 |
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| source | ACS Publications; MEDLINE |
| subjects | Animals Antineoplastic Agents, Phytogenic - administration & dosage Antineoplastic Agents, Phytogenic - chemistry Camptothecin - administration & dosage Camptothecin - chemistry Drug Compounding Female Humans Lung Neoplasms - drug therapy Lung Neoplasms - genetics Lung Neoplasms - metabolism Mice Mice, Inbred BALB C Molecular Targeted Therapy Nanostructures - administration & dosage Nanostructures - chemistry Plant Oils - chemistry Receptor, Epidermal Growth Factor - antagonists & inhibitors Receptor, Epidermal Growth Factor - genetics Receptor, Epidermal Growth Factor - metabolism |
| title | Development of Nanoscale Oil Bodies for Targeted Treatment of Lung Cancer |
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