Caries-arresting effects of silver diamine fluoride and sodium fluoride on dentine caries lesions
To investigate the remineralising effect and bacterial growth inhibition of 38% silver diamine fluoride (SDF) solution and 5% sodium fluoride (NaF) varnish on artificial dentine caries lesions. Demineralised dentine blocks were treated with SDF + NaF (Group 1), SDF (Group 2), NaF (Group 3) and water...
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| Veröffentlicht in: | Journal of dentistry 2018-11, Vol.78, p.65-71 |
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| creator | Yu, Ollie Y. Zhao, Irene S. Mei, May L. Lo, Edward C.M. Chu, C.H. |
| description | To investigate the remineralising effect and bacterial growth inhibition of 38% silver diamine fluoride (SDF) solution and 5% sodium fluoride (NaF) varnish on artificial dentine caries lesions.
Demineralised dentine blocks were treated with SDF + NaF (Group 1), SDF (Group 2), NaF (Group 3) and water (Group 4) and subjected to a Streptococcus mutans biofilm challenge. Lesion depth, precipitates’ characteristics and matrix (collagen)-to-mineral ratio were evaluated by micro-computer tomography (micro-CT), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. The biofilm kinetics, viability and topography were assessed by counts of colony forming units (CFUs), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), respectively. Data were analysed by two-way ANOVA test.
The lesion depths of Groups 1–4 were 170 ± 28 μm, 160 ± 32 μm, 353 ± 38 μm and 449 ± 24 μm, respectively. The addition of NaF to SDF did not show better remineralisation than SDF (p = 0.491). Metallic silver and silver chloride were found in Groups 1 and 2. The amide I-to-hydrogen phosphate ratios of the four groups were 0.14 ± 0.02, 0.14 ± 0.01, 0.29 ± 0.05 and 0.49 ± 0.16, respectively, and the addition of NaF to SDF did not offer better protection against collagen exposure than SDF (p = 0.986). The Log10 CFUs of Groups 1–4 were 5.75 ± 0.56, 4.49 ± 0.57, 6.55 ± 0.39 and 6.40 ± 0.38, respectively. The presence of NaF reduced the antibacterial effect of SDF (p |
| doi_str_mv | 10.1016/j.jdent.2018.08.007 |
| format | Article |
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Demineralised dentine blocks were treated with SDF + NaF (Group 1), SDF (Group 2), NaF (Group 3) and water (Group 4) and subjected to a Streptococcus mutans biofilm challenge. Lesion depth, precipitates’ characteristics and matrix (collagen)-to-mineral ratio were evaluated by micro-computer tomography (micro-CT), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. The biofilm kinetics, viability and topography were assessed by counts of colony forming units (CFUs), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), respectively. Data were analysed by two-way ANOVA test.
The lesion depths of Groups 1–4 were 170 ± 28 μm, 160 ± 32 μm, 353 ± 38 μm and 449 ± 24 μm, respectively. The addition of NaF to SDF did not show better remineralisation than SDF (p = 0.491). Metallic silver and silver chloride were found in Groups 1 and 2. The amide I-to-hydrogen phosphate ratios of the four groups were 0.14 ± 0.02, 0.14 ± 0.01, 0.29 ± 0.05 and 0.49 ± 0.16, respectively, and the addition of NaF to SDF did not offer better protection against collagen exposure than SDF (p = 0.986). The Log10 CFUs of Groups 1–4 were 5.75 ± 0.56, 4.49 ± 0.57, 6.55 ± 0.39 and 6.40 ± 0.38, respectively. The presence of NaF reduced the antibacterial effect of SDF (p < 0.001). The SEM and CLSM images supported the findings.
Application of SDF with or without NaF reduced the demineralisation of dentine caries, but SDF exerted stronger inhibition of biofilm growth than SDF with NaF.
NaF varnish affects the antibacterialeffects of SDF, the adjunctive application of SDF solution and NaF varnish is not recommended to arrest dentine caries in clinic.</description><identifier>ISSN: 0300-5712</identifier><identifier>EISSN: 1879-176X</identifier><identifier>DOI: 10.1016/j.jdent.2018.08.007</identifier><identifier>PMID: 30114443</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Bacteria ; Biofilms ; Caries ; Collagen ; Computed tomography ; Confocal microscopy ; Data processing ; Dental caries ; Dentine ; Dentistry ; Dentists ; Enamel ; Enzymes ; Fluorides ; Fourier transforms ; Infrared spectroscopy ; Lesions ; Medical imaging ; Precipitates ; Scanning electron microscopy ; Scanning microscopy ; Silver chloride ; Silver diamine fluoride ; Sodium ; Sodium fluoride ; Software ; Viability ; X-ray diffraction</subject><ispartof>Journal of dentistry, 2018-11, Vol.78, p.65-71</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Nov 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-30250f01c4ac59d7f18e8c79bb8edd02f98b7fe217908c37994a20cd502377703</citedby><cites>FETCH-LOGICAL-c453t-30250f01c4ac59d7f18e8c79bb8edd02f98b7fe217908c37994a20cd502377703</cites><orcidid>0000-0002-8167-0430 ; 0000-0002-3618-0619</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0300571218302951$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30114443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Ollie Y.</creatorcontrib><creatorcontrib>Zhao, Irene S.</creatorcontrib><creatorcontrib>Mei, May L.</creatorcontrib><creatorcontrib>Lo, Edward C.M.</creatorcontrib><creatorcontrib>Chu, C.H.</creatorcontrib><title>Caries-arresting effects of silver diamine fluoride and sodium fluoride on dentine caries lesions</title><title>Journal of dentistry</title><addtitle>J Dent</addtitle><description>To investigate the remineralising effect and bacterial growth inhibition of 38% silver diamine fluoride (SDF) solution and 5% sodium fluoride (NaF) varnish on artificial dentine caries lesions.
Demineralised dentine blocks were treated with SDF + NaF (Group 1), SDF (Group 2), NaF (Group 3) and water (Group 4) and subjected to a Streptococcus mutans biofilm challenge. Lesion depth, precipitates’ characteristics and matrix (collagen)-to-mineral ratio were evaluated by micro-computer tomography (micro-CT), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. The biofilm kinetics, viability and topography were assessed by counts of colony forming units (CFUs), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), respectively. Data were analysed by two-way ANOVA test.
The lesion depths of Groups 1–4 were 170 ± 28 μm, 160 ± 32 μm, 353 ± 38 μm and 449 ± 24 μm, respectively. The addition of NaF to SDF did not show better remineralisation than SDF (p = 0.491). Metallic silver and silver chloride were found in Groups 1 and 2. The amide I-to-hydrogen phosphate ratios of the four groups were 0.14 ± 0.02, 0.14 ± 0.01, 0.29 ± 0.05 and 0.49 ± 0.16, respectively, and the addition of NaF to SDF did not offer better protection against collagen exposure than SDF (p = 0.986). The Log10 CFUs of Groups 1–4 were 5.75 ± 0.56, 4.49 ± 0.57, 6.55 ± 0.39 and 6.40 ± 0.38, respectively. The presence of NaF reduced the antibacterial effect of SDF (p < 0.001). The SEM and CLSM images supported the findings.
Application of SDF with or without NaF reduced the demineralisation of dentine caries, but SDF exerted stronger inhibition of biofilm growth than SDF with NaF.
NaF varnish affects the antibacterialeffects of SDF, the adjunctive application of SDF solution and NaF varnish is not recommended to arrest dentine caries in clinic.</description><subject>Bacteria</subject><subject>Biofilms</subject><subject>Caries</subject><subject>Collagen</subject><subject>Computed tomography</subject><subject>Confocal microscopy</subject><subject>Data processing</subject><subject>Dental caries</subject><subject>Dentine</subject><subject>Dentistry</subject><subject>Dentists</subject><subject>Enamel</subject><subject>Enzymes</subject><subject>Fluorides</subject><subject>Fourier transforms</subject><subject>Infrared spectroscopy</subject><subject>Lesions</subject><subject>Medical imaging</subject><subject>Precipitates</subject><subject>Scanning electron microscopy</subject><subject>Scanning microscopy</subject><subject>Silver chloride</subject><subject>Silver diamine fluoride</subject><subject>Sodium</subject><subject>Sodium fluoride</subject><subject>Software</subject><subject>Viability</subject><subject>X-ray diffraction</subject><issn>0300-5712</issn><issn>1879-176X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kc1qHDEQhEWIiTdOniAQBLnkMuuWNGNJBx_Ckh-DwRcbchNaqRU0zEi2tGPw20frdWzIwdAgaL6ublUR8onBmgE7Ox3Xo8e0W3Ngag2tQL4hK6ak7pg8-_2WrEAAdINk_Ji8r3UEgB64fkeOBTDW971YEbuxJWLtbClYdzH9oRgCul2lOdAap3ss1Ec7x4Q0TEsu0SO1ydOafVzml15OdH_NnnOPknTCGnOqH8hRsFPFj0_vCbn58f1686u7vPp5sfl22bl-ELtOAB8gAHO9dYP2MjCFykm93Sr0HnjQaisDciY1KCek1r3l4PwAXEgpQZyQrwfd25LvlvYZM8fqcJpswrxUw0FpNUguVEO__IeOeSmpXWc4Y82vngndKHGgXMm1FgzmtsTZlgfDwOwTMKN5TMDsEzDQCmSb-vykvWxn9M8z_yxvwPkBwGbGfcRiqouYHPpYmvHG5_jqgr_UApfu</recordid><startdate>201811</startdate><enddate>201811</enddate><creator>Yu, Ollie Y.</creator><creator>Zhao, Irene S.</creator><creator>Mei, May L.</creator><creator>Lo, Edward C.M.</creator><creator>Chu, C.H.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8167-0430</orcidid><orcidid>https://orcid.org/0000-0002-3618-0619</orcidid></search><sort><creationdate>201811</creationdate><title>Caries-arresting effects of silver diamine fluoride and sodium fluoride on dentine caries lesions</title><author>Yu, Ollie Y. ; Zhao, Irene S. ; Mei, May L. ; Lo, Edward C.M. ; Chu, C.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-30250f01c4ac59d7f18e8c79bb8edd02f98b7fe217908c37994a20cd502377703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bacteria</topic><topic>Biofilms</topic><topic>Caries</topic><topic>Collagen</topic><topic>Computed tomography</topic><topic>Confocal microscopy</topic><topic>Data processing</topic><topic>Dental caries</topic><topic>Dentine</topic><topic>Dentistry</topic><topic>Dentists</topic><topic>Enamel</topic><topic>Enzymes</topic><topic>Fluorides</topic><topic>Fourier transforms</topic><topic>Infrared spectroscopy</topic><topic>Lesions</topic><topic>Medical imaging</topic><topic>Precipitates</topic><topic>Scanning electron microscopy</topic><topic>Scanning microscopy</topic><topic>Silver chloride</topic><topic>Silver diamine fluoride</topic><topic>Sodium</topic><topic>Sodium fluoride</topic><topic>Software</topic><topic>Viability</topic><topic>X-ray diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Ollie Y.</creatorcontrib><creatorcontrib>Zhao, Irene S.</creatorcontrib><creatorcontrib>Mei, May L.</creatorcontrib><creatorcontrib>Lo, Edward C.M.</creatorcontrib><creatorcontrib>Chu, C.H.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dentistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Ollie Y.</au><au>Zhao, Irene S.</au><au>Mei, May L.</au><au>Lo, Edward C.M.</au><au>Chu, C.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Caries-arresting effects of silver diamine fluoride and sodium fluoride on dentine caries lesions</atitle><jtitle>Journal of dentistry</jtitle><addtitle>J Dent</addtitle><date>2018-11</date><risdate>2018</risdate><volume>78</volume><spage>65</spage><epage>71</epage><pages>65-71</pages><issn>0300-5712</issn><eissn>1879-176X</eissn><abstract>To investigate the remineralising effect and bacterial growth inhibition of 38% silver diamine fluoride (SDF) solution and 5% sodium fluoride (NaF) varnish on artificial dentine caries lesions.
Demineralised dentine blocks were treated with SDF + NaF (Group 1), SDF (Group 2), NaF (Group 3) and water (Group 4) and subjected to a Streptococcus mutans biofilm challenge. Lesion depth, precipitates’ characteristics and matrix (collagen)-to-mineral ratio were evaluated by micro-computer tomography (micro-CT), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. The biofilm kinetics, viability and topography were assessed by counts of colony forming units (CFUs), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), respectively. Data were analysed by two-way ANOVA test.
The lesion depths of Groups 1–4 were 170 ± 28 μm, 160 ± 32 μm, 353 ± 38 μm and 449 ± 24 μm, respectively. The addition of NaF to SDF did not show better remineralisation than SDF (p = 0.491). Metallic silver and silver chloride were found in Groups 1 and 2. The amide I-to-hydrogen phosphate ratios of the four groups were 0.14 ± 0.02, 0.14 ± 0.01, 0.29 ± 0.05 and 0.49 ± 0.16, respectively, and the addition of NaF to SDF did not offer better protection against collagen exposure than SDF (p = 0.986). The Log10 CFUs of Groups 1–4 were 5.75 ± 0.56, 4.49 ± 0.57, 6.55 ± 0.39 and 6.40 ± 0.38, respectively. The presence of NaF reduced the antibacterial effect of SDF (p < 0.001). The SEM and CLSM images supported the findings.
Application of SDF with or without NaF reduced the demineralisation of dentine caries, but SDF exerted stronger inhibition of biofilm growth than SDF with NaF.
NaF varnish affects the antibacterialeffects of SDF, the adjunctive application of SDF solution and NaF varnish is not recommended to arrest dentine caries in clinic.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>30114443</pmid><doi>10.1016/j.jdent.2018.08.007</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-8167-0430</orcidid><orcidid>https://orcid.org/0000-0002-3618-0619</orcidid></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Bacteria Biofilms Caries Collagen Computed tomography Confocal microscopy Data processing Dental caries Dentine Dentistry Dentists Enamel Enzymes Fluorides Fourier transforms Infrared spectroscopy Lesions Medical imaging Precipitates Scanning electron microscopy Scanning microscopy Silver chloride Silver diamine fluoride Sodium Sodium fluoride Software Viability X-ray diffraction |
| title | Caries-arresting effects of silver diamine fluoride and sodium fluoride on dentine caries lesions |
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