Purification of His sub(6)-tagged Photosystem I from Chlamydomonas reinhardtii

We have developed a rapid method for isolation of the Photosystem I (PS1) complex from Chlamydomonas reinhardtii using epitope tagging. Six histidine residues were genetically added to the N-terminus of the PsaA core subunit of PS1. The His sub(6)-tagged PS1 could be purified with a yield of 80-90%...

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Veröffentlicht in:	Photosynthesis research 2008-04, Vol.96 (1), p.51-60
Hauptverfasser:	Gulis, Galina, Narasimhulu, Kuppala V, Fox, Lisa N, Redding, Kevin E
Format:	Artikel
Sprache:	eng
Schlagworte:	Chlamydomonas reinhardtii
Online-Zugang:	Volltext
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