Neutralizing assay of monoclonal antibodies against white spot syndrome virus (WSSV)
Monoclonal antibodies (Mabs) against white spot syndrome virus (WSSV) were tested for their ability to neutralize WSSV infectivity by immunodot-blot assay and enzyme-linked immunosorbent assay. It was proved that WSSV could bind to haemocyte membranes of Penaeus chinensis, moreover, the result showe...
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| Veröffentlicht in: | Aquaculture 2007-11, Vol.272 (1-4), p.216-222 |
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| creator | Jiang, You-sheng Zhan, Wen-bin Sheng, Xiu-zhen |
| description | Monoclonal antibodies (Mabs) against white spot syndrome virus (WSSV) were tested for their ability to neutralize WSSV infectivity by immunodot-blot assay and enzyme-linked immunosorbent assay. It was proved that WSSV could bind to haemocyte membranes of Penaeus chinensis, moreover, the result showed that the Mab 2D2 could reduce the binding of WSSV with haemocyte membranes of P. chinensis. The neutralization assay was further conducted in vitro with primary cultured haemocytes of P. chinensis, and the cell culture assay showed that the two monoclonal antibodies, 2D2 and 6B4 could partially neutralize WSSV infection. |
| doi_str_mv | 10.1016/j.aquaculture.2007.07.229 |
| format | Article |
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It was proved that WSSV could bind to haemocyte membranes of Penaeus chinensis, moreover, the result showed that the Mab 2D2 could reduce the binding of WSSV with haemocyte membranes of P. chinensis. The neutralization assay was further conducted in vitro with primary cultured haemocytes of P. chinensis, and the cell culture assay showed that the two monoclonal antibodies, 2D2 and 6B4 could partially neutralize WSSV infection.</description><identifier>ISSN: 0044-8486</identifier><identifier>EISSN: 1873-5622</identifier><identifier>DOI: 10.1016/j.aquaculture.2007.07.229</identifier><identifier>CODEN: AQCLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animal aquaculture ; Animal productions ; Aquaculture ; Biological and medical sciences ; enzyme-linked immunosorbent assay ; Fenneropenaeus chinensis ; Fundamental and applied biological sciences. Psychology ; General aspects ; Haemocyte membranes ; Immune system ; Immunoassay ; immunoblotting ; monoclonal antibodies ; Monoclonal antibody ; Neutralization ; neutralization tests ; Penaeus chinensis ; Shellfish ; shrimp ; shrimp culture ; Viruses ; white spot syndrome ; White spot syndrome virus</subject><ispartof>Aquaculture, 2007-11, Vol.272 (1-4), p.216-222</ispartof><rights>2007 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><rights>Copyright Elsevier Sequoia S.A. 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It was proved that WSSV could bind to haemocyte membranes of Penaeus chinensis, moreover, the result showed that the Mab 2D2 could reduce the binding of WSSV with haemocyte membranes of P. chinensis. The neutralization assay was further conducted in vitro with primary cultured haemocytes of P. chinensis, and the cell culture assay showed that the two monoclonal antibodies, 2D2 and 6B4 could partially neutralize WSSV infection.</description><subject>Animal aquaculture</subject><subject>Animal productions</subject><subject>Aquaculture</subject><subject>Biological and medical sciences</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Fenneropenaeus chinensis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Haemocyte membranes</subject><subject>Immune system</subject><subject>Immunoassay</subject><subject>immunoblotting</subject><subject>monoclonal antibodies</subject><subject>Monoclonal antibody</subject><subject>Neutralization</subject><subject>neutralization tests</subject><subject>Penaeus chinensis</subject><subject>Shellfish</subject><subject>shrimp</subject><subject>shrimp culture</subject><subject>Viruses</subject><subject>white spot syndrome</subject><subject>White spot syndrome virus</subject><issn>0044-8486</issn><issn>1873-5622</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNkM1uEzEURi1EJULKM2CQQGUxwX_jsZdVBAWpgkVaWFo3Hjs4mtipPVMUnh5HqUTVFdKVvDn38_0OQm8oWVBC5cftAu4msNMwTtktGCHdog5j-hmaUdXxppWMPUczQoRolFDyBXpZypYQImVLZ-jmm5vGDEP4E-IGQylwwMnjXYrJDinCgCGOYZ364AqGDYRYRvz7VxgdLvs04nKIfU47h-9Dngq--Lla_fhwjs48DMW9enjn6Pbzp5vll-b6-9XX5eV1YwXnY8M0lZ5Z7UFqzmgvlFZakg58D7bTrLey7VopoRXa27WXnadk7YXkrOO1AJ-j96fcfU53kyuj2YVi3TBAdGkqhhGlWSdYBd8-AbdpyrXdkREdJaqqmiN9gmxOpWTnzT6HHeSDocQcZZuteSTbHGWbOlV23X338AEUC4PPEG0o_wI0rxe3pHKvT5yHZGCTK3O7YoTWOoorRo5JyxPhqrj74LIpNrhoXR-ys6PpU_iPe_4CDOakOQ</recordid><startdate>20071126</startdate><enddate>20071126</enddate><creator>Jiang, You-sheng</creator><creator>Zhan, Wen-bin</creator><creator>Sheng, Xiu-zhen</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier Sequoia S.A</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20071126</creationdate><title>Neutralizing assay of monoclonal antibodies against white spot syndrome virus (WSSV)</title><author>Jiang, You-sheng ; Zhan, Wen-bin ; Sheng, Xiu-zhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-2916f2c9fa69321d48989607afdac792dc657566a549fcbf67f10bf4632730063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animal aquaculture</topic><topic>Animal productions</topic><topic>Aquaculture</topic><topic>Biological and medical sciences</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Fenneropenaeus chinensis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Haemocyte membranes</topic><topic>Immune system</topic><topic>Immunoassay</topic><topic>immunoblotting</topic><topic>monoclonal antibodies</topic><topic>Monoclonal antibody</topic><topic>Neutralization</topic><topic>neutralization tests</topic><topic>Penaeus chinensis</topic><topic>Shellfish</topic><topic>shrimp</topic><topic>shrimp culture</topic><topic>Viruses</topic><topic>white spot syndrome</topic><topic>White spot syndrome virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, You-sheng</creatorcontrib><creatorcontrib>Zhan, Wen-bin</creatorcontrib><creatorcontrib>Sheng, Xiu-zhen</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Aquaculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, You-sheng</au><au>Zhan, Wen-bin</au><au>Sheng, Xiu-zhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutralizing assay of monoclonal antibodies against white spot syndrome virus (WSSV)</atitle><jtitle>Aquaculture</jtitle><date>2007-11-26</date><risdate>2007</risdate><volume>272</volume><issue>1-4</issue><spage>216</spage><epage>222</epage><pages>216-222</pages><issn>0044-8486</issn><eissn>1873-5622</eissn><coden>AQCLAL</coden><abstract>Monoclonal antibodies (Mabs) against white spot syndrome virus (WSSV) were tested for their ability to neutralize WSSV infectivity by immunodot-blot assay and enzyme-linked immunosorbent assay. It was proved that WSSV could bind to haemocyte membranes of Penaeus chinensis, moreover, the result showed that the Mab 2D2 could reduce the binding of WSSV with haemocyte membranes of P. chinensis. The neutralization assay was further conducted in vitro with primary cultured haemocytes of P. chinensis, and the cell culture assay showed that the two monoclonal antibodies, 2D2 and 6B4 could partially neutralize WSSV infection.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.aquaculture.2007.07.229</doi><tpages>7</tpages></addata></record> |
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| ispartof | Aquaculture, 2007-11, Vol.272 (1-4), p.216-222 |
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| source | Elsevier ScienceDirect Journals |
| subjects | Animal aquaculture Animal productions Aquaculture Biological and medical sciences enzyme-linked immunosorbent assay Fenneropenaeus chinensis Fundamental and applied biological sciences. Psychology General aspects Haemocyte membranes Immune system Immunoassay immunoblotting monoclonal antibodies Monoclonal antibody Neutralization neutralization tests Penaeus chinensis Shellfish shrimp shrimp culture Viruses white spot syndrome White spot syndrome virus |
| title | Neutralizing assay of monoclonal antibodies against white spot syndrome virus (WSSV) |
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