Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study
Mitochondria perform crucial roles in many biochemical processes, and mitochondrial depolarization is an early sign of platelet apoptosis. The mitochondrial membrane potential is usually evaluated through JC‐1 probe, but it can also be assessed with MitoTracker probes. Our aim was to evaluate mitoch...
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| Veröffentlicht in: | Cytometry. Part A 2019-02, Vol.95 (2), p.214-218 |
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| creator | Marcondes, Natália Aydos Terra, Silvia Resende Lasta, Camila Serina Hlavac, Nicole Regina Capacchi Dalmolin, Magnus Larruscaim Lacerda, Luciana de Almeida Faulhaber, Gustavo Adolpho Moreira González, Félix Hilário Díaz |
| description | Mitochondria perform crucial roles in many biochemical processes, and mitochondrial depolarization is an early sign of platelet apoptosis. The mitochondrial membrane potential is usually evaluated through JC‐1 probe, but it can also be assessed with MitoTracker probes. Our aim was to evaluate mitochondrial viability in stored canine platelet concentrates (PCs) with the fluorescent probes JC‐1 and MitoTracker. Platelets from 22 canine PCs were stained with JC‐1 and MitoTracker probes on days 1, 3, and 5 of storage. Data on metabolic parameters were also collected for correlation studies. Results of JC‐1 and MitoTracker revealed a decrease in mitochondrial membrane potential in day 5 of storage compared to days 1 and 3, providing evidence of mitochondrial depolarization, a finding that was confirmed by the data on metabolic parameters. MitoTracker probes also added information regarding platelet swelling. In conclusion, MitoTracker probes offered a more complete mitochondrial analysis in the evaluation of stored canine PCs. © 2018 International Society for Advancement of Cytometry |
| doi_str_mv | 10.1002/cyto.a.23567 |
| format | Article |
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The mitochondrial membrane potential is usually evaluated through JC‐1 probe, but it can also be assessed with MitoTracker probes. Our aim was to evaluate mitochondrial viability in stored canine platelet concentrates (PCs) with the fluorescent probes JC‐1 and MitoTracker. Platelets from 22 canine PCs were stained with JC‐1 and MitoTracker probes on days 1, 3, and 5 of storage. Data on metabolic parameters were also collected for correlation studies. Results of JC‐1 and MitoTracker revealed a decrease in mitochondrial membrane potential in day 5 of storage compared to days 1 and 3, providing evidence of mitochondrial depolarization, a finding that was confirmed by the data on metabolic parameters. MitoTracker probes also added information regarding platelet swelling. In conclusion, MitoTracker probes offered a more complete mitochondrial analysis in the evaluation of stored canine PCs. © 2018 International Society for Advancement of Cytometry</description><identifier>ISSN: 1552-4922</identifier><identifier>EISSN: 1552-4930</identifier><identifier>DOI: 10.1002/cyto.a.23567</identifier><identifier>PMID: 30107098</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Animals ; Apoptosis ; Apoptosis - physiology ; Benzimidazoles - metabolism ; Blood Platelets - metabolism ; Blood Preservation - methods ; Carbocyanines - metabolism ; Correlation analysis ; Depolarization ; Dogs ; Flow cytometry ; Flow Cytometry - methods ; Fluorescent Dyes - metabolism ; Fluorescent indicators ; JC‐1 ; Membrane potential ; Membrane Potential, Mitochondrial - physiology ; Metabolism ; Mitochondria ; Mitochondria - metabolism ; mitochondrial membrane potential ; MitoTracker ; Parameters ; platelet storage lesion ; Platelets ; Probes ; Viability</subject><ispartof>Cytometry. 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Part A</title><addtitle>Cytometry A</addtitle><description>Mitochondria perform crucial roles in many biochemical processes, and mitochondrial depolarization is an early sign of platelet apoptosis. The mitochondrial membrane potential is usually evaluated through JC‐1 probe, but it can also be assessed with MitoTracker probes. Our aim was to evaluate mitochondrial viability in stored canine platelet concentrates (PCs) with the fluorescent probes JC‐1 and MitoTracker. Platelets from 22 canine PCs were stained with JC‐1 and MitoTracker probes on days 1, 3, and 5 of storage. Data on metabolic parameters were also collected for correlation studies. Results of JC‐1 and MitoTracker revealed a decrease in mitochondrial membrane potential in day 5 of storage compared to days 1 and 3, providing evidence of mitochondrial depolarization, a finding that was confirmed by the data on metabolic parameters. MitoTracker probes also added information regarding platelet swelling. In conclusion, MitoTracker probes offered a more complete mitochondrial analysis in the evaluation of stored canine PCs. © 2018 International Society for Advancement of Cytometry</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - physiology</subject><subject>Benzimidazoles - metabolism</subject><subject>Blood Platelets - metabolism</subject><subject>Blood Preservation - methods</subject><subject>Carbocyanines - metabolism</subject><subject>Correlation analysis</subject><subject>Depolarization</subject><subject>Dogs</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - methods</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Fluorescent indicators</subject><subject>JC‐1</subject><subject>Membrane potential</subject><subject>Membrane Potential, Mitochondrial - physiology</subject><subject>Metabolism</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>mitochondrial membrane potential</subject><subject>MitoTracker</subject><subject>Parameters</subject><subject>platelet storage lesion</subject><subject>Platelets</subject><subject>Probes</subject><subject>Viability</subject><issn>1552-4922</issn><issn>1552-4930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kbtuFDEUhkeIiFygo0aWaCiyiy9zM91qxC0KSrMUVNYZz7Fw8NiLPUM0HY8Ar5gnwcsmKSiozkXf-XV-_UXxnNE1o5S_1ssU1rDmoqqbR8UJqyq-KqWgjx96zo-L05SuKRUVFfxJcSwoow2V7UnxuwvjDqJNwZNgyEV3-_MXI-AH8slOYRtBf8NIdjH0mIgJkYx5rb8GP0QLjvyw0Ftnp4VASpjSiH4i1pM0hYgD0eCtR7JzMKHDiejgdSZiHtMbsiHGhRuyNzDiFJd8NQ_L0-LIgEv47K6eFZ_fvd12H1aXV-8_dpvLlS4pb1a1qKk0EkA30nANbcuxb0ohTKXLWpSi1KwfqIAeesZBUjMgE7LG1hiUmoqz4tVBN5v7PmOa1GiTRufAY5iT4rRtm4qXssroy3_Q6zBHn79TnDWSyZbLJlPnB0rHkFJEo3bRjhAXxajaR6X2ThWov1Fl_MWd6NyPODzA99lkoDwAN9bh8l8x1X3ZXm0Oun8A-VykCQ</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Marcondes, Natália Aydos</creator><creator>Terra, Silvia Resende</creator><creator>Lasta, Camila Serina</creator><creator>Hlavac, Nicole Regina Capacchi</creator><creator>Dalmolin, Magnus Larruscaim</creator><creator>Lacerda, Luciana de Almeida</creator><creator>Faulhaber, Gustavo Adolpho Moreira</creator><creator>González, Félix Hilário Díaz</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201902</creationdate><title>Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study</title><author>Marcondes, Natália Aydos ; Terra, Silvia Resende ; Lasta, Camila Serina ; Hlavac, Nicole Regina Capacchi ; Dalmolin, Magnus Larruscaim ; Lacerda, Luciana de Almeida ; Faulhaber, Gustavo Adolpho Moreira ; González, Félix Hilário Díaz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4027-63609f9aac79f2ca882eb7433f5c463434c1bd03abab12a90fde1396e8ffe9c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - physiology</topic><topic>Benzimidazoles - metabolism</topic><topic>Blood Platelets - metabolism</topic><topic>Blood Preservation - methods</topic><topic>Carbocyanines - metabolism</topic><topic>Correlation analysis</topic><topic>Depolarization</topic><topic>Dogs</topic><topic>Flow cytometry</topic><topic>Flow Cytometry - methods</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Fluorescent indicators</topic><topic>JC‐1</topic><topic>Membrane potential</topic><topic>Membrane Potential, Mitochondrial - physiology</topic><topic>Metabolism</topic><topic>Mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>mitochondrial membrane potential</topic><topic>MitoTracker</topic><topic>Parameters</topic><topic>platelet storage lesion</topic><topic>Platelets</topic><topic>Probes</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marcondes, Natália Aydos</creatorcontrib><creatorcontrib>Terra, Silvia Resende</creatorcontrib><creatorcontrib>Lasta, Camila Serina</creatorcontrib><creatorcontrib>Hlavac, Nicole Regina Capacchi</creatorcontrib><creatorcontrib>Dalmolin, Magnus Larruscaim</creatorcontrib><creatorcontrib>Lacerda, Luciana de Almeida</creatorcontrib><creatorcontrib>Faulhaber, Gustavo Adolpho Moreira</creatorcontrib><creatorcontrib>González, Félix Hilário Díaz</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cytometry. 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| ispartof | Cytometry. Part A, 2019-02, Vol.95 (2), p.214-218 |
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| source | MEDLINE; Wiley Free Content; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Animals Apoptosis Apoptosis - physiology Benzimidazoles - metabolism Blood Platelets - metabolism Blood Preservation - methods Carbocyanines - metabolism Correlation analysis Depolarization Dogs Flow cytometry Flow Cytometry - methods Fluorescent Dyes - metabolism Fluorescent indicators JC‐1 Membrane potential Membrane Potential, Mitochondrial - physiology Metabolism Mitochondria Mitochondria - metabolism mitochondrial membrane potential MitoTracker Parameters platelet storage lesion Platelets Probes Viability |
| title | Comparison of JC‐1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study |
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