Gemfibrozil increases the specific binding of rat-cortex nuclear extracts to a PPRE probe
PPAR agonists have been shown to elicit beneficial responses in several cell- and tissue-models of neurotoxicity. To determine if brain PPARs are responsive to the in vivo administration of PPAR agonists in a similar way to those receptors present in other anatomical localizations, such as liver, we...
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| Veröffentlicht in: | Life sciences (1973) 2003-10, Vol.73 (23), p.2927-2937 |
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| container_end_page | 2937 |
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| container_issue | 23 |
| container_start_page | 2927 |
| container_title | Life sciences (1973) |
| container_volume | 73 |
| creator | Sanguino, Elena Ramón, Miguel Roglans, Núria Alegret, Marta Sánchez, Rosa M Vázquez-Carrera, Manuel Laguna, Juan C |
| description | PPAR agonists have been shown to elicit beneficial responses in several cell- and tissue-models of neurotoxicity. To determine if brain PPARs are responsive to the in vivo administration of PPAR agonists in a similar way to those receptors present in other anatomical localizations, such as liver, we fed rats with gemfibrozil incorporated in the diet at a dose that activates hepatic PPARα and produces its typical hypolipidemic effect. Rat cortex nuclear extracts presented a pattern of two specific shifted bands when incubated with a PPRE oligonucleotide. Samples from gemfibrozil-treated rats showed a significant increase in the intensity of the two shifted bands regarding control values (2.4- and 1.8-fold for the specific bands 1 and 2, respectively), indicating that orally administered gemfibrozil reaches brain tissues at concentrations sufficient to increase the specific binding of cortex nuclear extracts to an oligonucleotide mimicking a
bona fide PPRE, although no changes in cortex ACO mRNA levels were produced. |
| doi_str_mv | 10.1016/j.lfs.2003.04.001 |
| format | Article |
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bona fide PPRE, although no changes in cortex ACO mRNA levels were produced.</description><subject>ACO</subject><subject>Acyl-CoA Oxidase</subject><subject>Administration, Oral</subject><subject>Animals</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - metabolism</subject><subject>Cerebral Cortex - drug effects</subject><subject>Cerebral Cortex - metabolism</subject><subject>Fibrates</subject><subject>Gemfibrozil</subject><subject>Gemfibrozil - administration & dosage</subject><subject>Gemfibrozil - pharmacology</subject><subject>Hypolipidemic Agents - administration & dosage</subject><subject>Hypolipidemic Agents - pharmacology</subject><subject>Male</subject><subject>Oxidoreductases - metabolism</subject><subject>PPAR</subject><subject>Rat cortex</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Cytoplasmic and Nuclear - genetics</subject><subject>Receptors, Cytoplasmic and Nuclear - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LwzAYh4MoOqcfwIvk5K31TZqkLZ5kzD8wUEQPnkKavtWMrp1JK-qnN2MDb55yeX7PSx5CzhikDJi6XKZtE1IOkKUgUgC2RyasyMsEVMb2yQSAiyTjII_IcQhLAJAyzw7JEROSlULwCXm9xVXjKt__uJa6zno0AQMd3pGGNVrXOEsr19Wue6N9Q70ZEtv7Ab9oN9oWjaf4NXhjh7jpqaGPj09zuvZ9hSfkoDFtwNPdOyUvN_Pn2V2yeLi9n10vEpsVEG3ASzDSKKmsgrzKeF4XpmlQCoVScqOYKUtkpcplxSoubVmrXAhgVZljobIpudh649WPEcOgVy5YbFvTYT8GzaEoJOQQQbYFre9D8NjotXcr4781A73pqZc69tSbnhqEjj3j5nwnH6sV1n-LXcAIXG0BjF_8dOh1sA47i7XzaAdd9-4f_S8cvoUe</recordid><startdate>20031024</startdate><enddate>20031024</enddate><creator>Sanguino, Elena</creator><creator>Ramón, Miguel</creator><creator>Roglans, Núria</creator><creator>Alegret, Marta</creator><creator>Sánchez, Rosa M</creator><creator>Vázquez-Carrera, Manuel</creator><creator>Laguna, Juan C</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20031024</creationdate><title>Gemfibrozil increases the specific binding of rat-cortex nuclear extracts to a PPRE probe</title><author>Sanguino, Elena ; Ramón, Miguel ; Roglans, Núria ; Alegret, Marta ; Sánchez, Rosa M ; Vázquez-Carrera, Manuel ; Laguna, Juan C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-c0290a5a656c607b327d8affe546e552a61a99e19675b1b25c9d674401b97e863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>ACO</topic><topic>Acyl-CoA Oxidase</topic><topic>Administration, Oral</topic><topic>Animals</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Cerebral Cortex - drug effects</topic><topic>Cerebral Cortex - metabolism</topic><topic>Fibrates</topic><topic>Gemfibrozil</topic><topic>Gemfibrozil - administration & dosage</topic><topic>Gemfibrozil - pharmacology</topic><topic>Hypolipidemic Agents - administration & dosage</topic><topic>Hypolipidemic Agents - pharmacology</topic><topic>Male</topic><topic>Oxidoreductases - metabolism</topic><topic>PPAR</topic><topic>Rat cortex</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Cytoplasmic and Nuclear - genetics</topic><topic>Receptors, Cytoplasmic and Nuclear - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanguino, Elena</creatorcontrib><creatorcontrib>Ramón, Miguel</creatorcontrib><creatorcontrib>Roglans, Núria</creatorcontrib><creatorcontrib>Alegret, Marta</creatorcontrib><creatorcontrib>Sánchez, Rosa M</creatorcontrib><creatorcontrib>Vázquez-Carrera, Manuel</creatorcontrib><creatorcontrib>Laguna, Juan C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanguino, Elena</au><au>Ramón, Miguel</au><au>Roglans, Núria</au><au>Alegret, Marta</au><au>Sánchez, Rosa M</au><au>Vázquez-Carrera, Manuel</au><au>Laguna, Juan C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gemfibrozil increases the specific binding of rat-cortex nuclear extracts to a PPRE probe</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2003-10-24</date><risdate>2003</risdate><volume>73</volume><issue>23</issue><spage>2927</spage><epage>2937</epage><pages>2927-2937</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>PPAR agonists have been shown to elicit beneficial responses in several cell- and tissue-models of neurotoxicity. To determine if brain PPARs are responsive to the in vivo administration of PPAR agonists in a similar way to those receptors present in other anatomical localizations, such as liver, we fed rats with gemfibrozil incorporated in the diet at a dose that activates hepatic PPARα and produces its typical hypolipidemic effect. Rat cortex nuclear extracts presented a pattern of two specific shifted bands when incubated with a PPRE oligonucleotide. Samples from gemfibrozil-treated rats showed a significant increase in the intensity of the two shifted bands regarding control values (2.4- and 1.8-fold for the specific bands 1 and 2, respectively), indicating that orally administered gemfibrozil reaches brain tissues at concentrations sufficient to increase the specific binding of cortex nuclear extracts to an oligonucleotide mimicking a
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| subjects | ACO Acyl-CoA Oxidase Administration, Oral Animals Cell Nucleus - drug effects Cell Nucleus - metabolism Cerebral Cortex - drug effects Cerebral Cortex - metabolism Fibrates Gemfibrozil Gemfibrozil - administration & dosage Gemfibrozil - pharmacology Hypolipidemic Agents - administration & dosage Hypolipidemic Agents - pharmacology Male Oxidoreductases - metabolism PPAR Rat cortex Rats Rats, Sprague-Dawley Receptors, Cytoplasmic and Nuclear - genetics Receptors, Cytoplasmic and Nuclear - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Transcription Factors - genetics Transcription Factors - metabolism |
| title | Gemfibrozil increases the specific binding of rat-cortex nuclear extracts to a PPRE probe |
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