The Hidden World of Rickettsiales Symbionts: “Candidatus Spectririckettsia obscura,” a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum
Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, includ...
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| Veröffentlicht in: | Microbial ecology 2019-04, Vol.77 (3), p.748-758 |
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| creator | Castelli, Michele Serra, Valentina Senra, Marcus V. X. Basuri, Charan K. Soares, Carlos A. G. Fokin, Sergei I. Modeo, Letizia Petroni, Giulio |
| description | Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, including several unicellular organisms, such as ciliates and amoebas. In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas. Phylogenetic analyses based on 16S rRNA gene showed that the two symbionts are closely related to each other (99.4% identity), belong to the family Rickettsiaceae, but are far-related with respect to previously characterized Rickettsiales. Consequently, they were assigned to a new species of a novel genus, namely “Candidatus Spectririckettsia obscura.” Screening on a database of short reads from 16S rRNA gene ampliconbased profiling studies confirmed that bacterial sequences related to the new symbiont are preferentially retrieved from freshwater environments, apparently with extremely scarce occurrence (< 0.1% positive samples). The present work provides new information on the still under-explored biodiversity of Rickettsiales, in particular those associated to ciliate host cells. |
| doi_str_mv | 10.1007/s00248-018-1243-8 |
| format | Article |
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X. ; Basuri, Charan K. ; Soares, Carlos A. G. ; Fokin, Sergei I. ; Modeo, Letizia ; Petroni, Giulio</creator><creatorcontrib>Castelli, Michele ; Serra, Valentina ; Senra, Marcus V. X. ; Basuri, Charan K. ; Soares, Carlos A. G. ; Fokin, Sergei I. ; Modeo, Letizia ; Petroni, Giulio</creatorcontrib><description>Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, including several unicellular organisms, such as ciliates and amoebas. In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas. Phylogenetic analyses based on 16S rRNA gene showed that the two symbionts are closely related to each other (99.4% identity), belong to the family Rickettsiaceae, but are far-related with respect to previously characterized Rickettsiales. Consequently, they were assigned to a new species of a novel genus, namely “Candidatus Spectririckettsia obscura.” Screening on a database of short reads from 16S rRNA gene ampliconbased profiling studies confirmed that bacterial sequences related to the new symbiont are preferentially retrieved from freshwater environments, apparently with extremely scarce occurrence (< 0.1% positive samples). The present work provides new information on the still under-explored biodiversity of Rickettsiales, in particular those associated to ciliate host cells.</description><identifier>ISSN: 0095-3628</identifier><identifier>EISSN: 1432-184X</identifier><identifier>DOI: 10.1007/s00248-018-1243-8</identifier><identifier>PMID: 30105505</identifier><language>eng</language><publisher>New York: Springer Science + Business Media</publisher><subject>Alphaproteobacteria ; Bacteria ; Biodiversity ; Biomedical and Life Sciences ; Candidatus Spectririckettsia obscura ; Ciliates ; Ecology ; Eukaryotes ; Freshwater ; Freshwater environments ; Freshwater organisms ; Gene sequencing ; Genotypes ; Geoecology/Natural Processes ; HOST MICROBE INTERACTIONS ; Inland water environment ; Life Sciences ; Microbial Ecology ; Microbiology ; Nature Conservation ; New species ; Paramecium caudatum ; Phylogeny ; Protozoa ; Rickettsiales ; rRNA 16S ; Symbionts ; Symbiosis ; Water Quality/Water Pollution</subject><ispartof>Microbial ecology, 2019-04, Vol.77 (3), p.748-758</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018. Corrected publication August/2018</rights><rights>Microbial Ecology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-27850988a287535d08d02df97c31eddb7b1c7a63beefb6de2b0910cbec0ff45c3</citedby><cites>FETCH-LOGICAL-c394t-27850988a287535d08d02df97c31eddb7b1c7a63beefb6de2b0910cbec0ff45c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/48702325$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/48702325$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,782,786,805,27931,27932,41495,42564,51326,58024,58257</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30105505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castelli, Michele</creatorcontrib><creatorcontrib>Serra, Valentina</creatorcontrib><creatorcontrib>Senra, Marcus V. 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In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas. Phylogenetic analyses based on 16S rRNA gene showed that the two symbionts are closely related to each other (99.4% identity), belong to the family Rickettsiaceae, but are far-related with respect to previously characterized Rickettsiales. Consequently, they were assigned to a new species of a novel genus, namely “Candidatus Spectririckettsia obscura.” Screening on a database of short reads from 16S rRNA gene ampliconbased profiling studies confirmed that bacterial sequences related to the new symbiont are preferentially retrieved from freshwater environments, apparently with extremely scarce occurrence (< 0.1% positive samples). The present work provides new information on the still under-explored biodiversity of Rickettsiales, in particular those associated to ciliate host cells.</description><subject>Alphaproteobacteria</subject><subject>Bacteria</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Candidatus Spectririckettsia obscura</subject><subject>Ciliates</subject><subject>Ecology</subject><subject>Eukaryotes</subject><subject>Freshwater</subject><subject>Freshwater environments</subject><subject>Freshwater organisms</subject><subject>Gene sequencing</subject><subject>Genotypes</subject><subject>Geoecology/Natural Processes</subject><subject>HOST MICROBE INTERACTIONS</subject><subject>Inland water environment</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Nature Conservation</subject><subject>New species</subject><subject>Paramecium caudatum</subject><subject>Phylogeny</subject><subject>Protozoa</subject><subject>Rickettsiales</subject><subject>rRNA 16S</subject><subject>Symbionts</subject><subject>Symbiosis</subject><subject>Water Quality/Water Pollution</subject><issn>0095-3628</issn><issn>1432-184X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1LxDAQhoMo7rr6AzwoBS-CVCdJ06RHWdQVFgRd0VtoPqpd22ZN2sP-e7t0VfDgaQ7zvO8MD0LHGC4xAL8KACQRMWARY5LQWOygMU4oibFIXnfRGCBjMU2JGKGDEJYAmKeE7qMRBQyMARuji8W7jWalMbaJXpyvTOSK6LHUH7ZtQ5lXNkRP61qVrmnDIdor8irYo-2coOfbm8V0Fs8f7u6n1_NY0yxpY8IFg0yInAjOKDMgDBBTZFxTbI1RXGHN85QqawuVGksUZBi0shqKImGaTtD50Lvy7rOzoZV1GbStqryxrguSgBAkYzzjPXr2B126zjf9dxuKc5FxTnsKD5T2LgRvC7nyZZ37tcQgNyblYFL2JuXGpBR95nTb3Knamp_Et7oeIAMQ-lXzZv3v6f9aT4bQMrTO_5QmggOhhNEvTlSF3g</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Castelli, Michele</creator><creator>Serra, Valentina</creator><creator>Senra, Marcus V. 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X.</au><au>Basuri, Charan K.</au><au>Soares, Carlos A. G.</au><au>Fokin, Sergei I.</au><au>Modeo, Letizia</au><au>Petroni, Giulio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Hidden World of Rickettsiales Symbionts: “Candidatus Spectririckettsia obscura,” a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum</atitle><jtitle>Microbial ecology</jtitle><stitle>Microb Ecol</stitle><addtitle>Microb Ecol</addtitle><date>2019-04-01</date><risdate>2019</risdate><volume>77</volume><issue>3</issue><spage>748</spage><epage>758</epage><pages>748-758</pages><issn>0095-3628</issn><eissn>1432-184X</eissn><abstract>Symbioses between bacteria and eukaryotes are widespread and may have significant impact on the evolutionary history of symbiotic partners. The order Rickettsiales is a lineage of intracellular Alphaproteobacteria characterized by an obligate association with a wide range of eukaryotic hosts, including several unicellular organisms, such as ciliates and amoebas. In this work, we characterized the Rickettsiales symbionts associated with two different genotypes of the freshwater ciliate Paramecium caudatum originated from freshwater environments in distant geographical areas. Phylogenetic analyses based on 16S rRNA gene showed that the two symbionts are closely related to each other (99.4% identity), belong to the family Rickettsiaceae, but are far-related with respect to previously characterized Rickettsiales. Consequently, they were assigned to a new species of a novel genus, namely “Candidatus Spectririckettsia obscura.” Screening on a database of short reads from 16S rRNA gene ampliconbased profiling studies confirmed that bacterial sequences related to the new symbiont are preferentially retrieved from freshwater environments, apparently with extremely scarce occurrence (< 0.1% positive samples). The present work provides new information on the still under-explored biodiversity of Rickettsiales, in particular those associated to ciliate host cells.</abstract><cop>New York</cop><pub>Springer Science + Business Media</pub><pmid>30105505</pmid><doi>10.1007/s00248-018-1243-8</doi><tpages>11</tpages></addata></record> |
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| ispartof | Microbial ecology, 2019-04, Vol.77 (3), p.748-758 |
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| subjects | Alphaproteobacteria Bacteria Biodiversity Biomedical and Life Sciences Candidatus Spectririckettsia obscura Ciliates Ecology Eukaryotes Freshwater Freshwater environments Freshwater organisms Gene sequencing Genotypes Geoecology/Natural Processes HOST MICROBE INTERACTIONS Inland water environment Life Sciences Microbial Ecology Microbiology Nature Conservation New species Paramecium caudatum Phylogeny Protozoa Rickettsiales rRNA 16S Symbionts Symbiosis Water Quality/Water Pollution |
| title | The Hidden World of Rickettsiales Symbionts: “Candidatus Spectririckettsia obscura,” a Novel Bacterium Found in Brazilian and Indian Paramecium caudatum |
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