Characterization of a motif for specific binding to hyaluronan in chicken SPACR
The chicken sialoprotein associated with cones and rods (SPACR) binds to hyaluronan (HA) in the interphotoreceptor matrix space, but the motif for HA binding is still unknown. This study was conducted to determine the critical site required for specific binding to HA. Western blotting study showed t...
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| Veröffentlicht in: | Journal of neurochemistry 2008-08, Vol.106 (3), p.1117-1124 |
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| creator | Zhao, Jinsong Yoneda, Masahiko Takeyama, Masayuki Miyaishi, Osamu Inoue, Yoko Kataoka, Takuya Ohno-Jinno, Akiko Isogai, Zenzo Kimata, Koji Iwaki, Masayoshi Zako, Masahiro |
| description | The chicken sialoprotein associated with cones and rods (SPACR) binds to hyaluronan (HA) in the interphotoreceptor matrix space, but the motif for HA binding is still unknown. This study was conducted to determine the critical site required for specific binding to HA. Western blotting study showed that SPACR binds biotinylated HA, and this interaction was specifically inhibited by unlabeled HA. A series of GST fusion proteins covering whole SPACR was prepared, and reactivity with HA was individually screened to narrow down the region for the binding. Further, putative HA-binding motif found near the carboxyl-terminus of SPACR was mutated by site-directed mutagenesis to identify the critical binding site. Finally, we showed that native SPACR derived from retina similarly binds to HA-affinity column under both reducing and non-reducing conditions. These results revealed that the specific putative HA-binding motif is located near the carboxyl-terminus of chicken SPACR, and suggested that a structural integrity such as folded structure is not largely involved in the HA binding. |
| doi_str_mv | 10.1111/j.1471-4159.2008.05468.x |
| format | Article |
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This study was conducted to determine the critical site required for specific binding to HA. Western blotting study showed that SPACR binds biotinylated HA, and this interaction was specifically inhibited by unlabeled HA. A series of GST fusion proteins covering whole SPACR was prepared, and reactivity with HA was individually screened to narrow down the region for the binding. Further, putative HA-binding motif found near the carboxyl-terminus of SPACR was mutated by site-directed mutagenesis to identify the critical binding site. Finally, we showed that native SPACR derived from retina similarly binds to HA-affinity column under both reducing and non-reducing conditions. These results revealed that the specific putative HA-binding motif is located near the carboxyl-terminus of chicken SPACR, and suggested that a structural integrity such as folded structure is not largely involved in the HA binding.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.2008.05468.x</identifier><identifier>PMID: 18466325</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Aminoacid receptors (glycine, glutamate, gaba) ; Animals ; Binding sites ; Binding Sites - physiology ; Biochemistry ; Biological and medical sciences ; Cell receptors ; Cell structures and functions ; Chickens ; Cytoskeleton, cytoplasm. Intracellular movements ; Extracellular Matrix Proteins - genetics ; Extracellular Matrix Proteins - metabolism ; Eye Proteins - genetics ; Eye Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; hyaluronan ; hyaluronan-binding motif ; Hyaluronic Acid - genetics ; Hyaluronic Acid - metabolism ; interphotoreceptor matrix ; Molecular and cellular biology ; Neurology ; Protein Interaction Domains and Motifs - physiology ; Proteins ; Proteoglycans - genetics ; Proteoglycans - metabolism ; receptor for hyaluronan-mediated motility ; Retina ; Retina - metabolism ; Sialoglycoproteins - genetics ; Sialoglycoproteins - metabolism ; sialoprotein associated with cones and rods</subject><ispartof>Journal of neurochemistry, 2008-08, Vol.106 (3), p.1117-1124</ispartof><rights>2008 The Authors. Journal Compilation © 2008 International Society for Neurochemistry</rights><rights>2008 INIST-CNRS</rights><rights>Journal compilation © 2008 International Society for Neurochemistry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4798-71d7e9e54ffa92b77748cec4294148f2159ba9d11f451bdd1d750da1f145dbb3</citedby><cites>FETCH-LOGICAL-c4798-71d7e9e54ffa92b77748cec4294148f2159ba9d11f451bdd1d750da1f145dbb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.2008.05468.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.2008.05468.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20525395$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18466325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Jinsong</creatorcontrib><creatorcontrib>Yoneda, Masahiko</creatorcontrib><creatorcontrib>Takeyama, Masayuki</creatorcontrib><creatorcontrib>Miyaishi, Osamu</creatorcontrib><creatorcontrib>Inoue, Yoko</creatorcontrib><creatorcontrib>Kataoka, Takuya</creatorcontrib><creatorcontrib>Ohno-Jinno, Akiko</creatorcontrib><creatorcontrib>Isogai, Zenzo</creatorcontrib><creatorcontrib>Kimata, Koji</creatorcontrib><creatorcontrib>Iwaki, Masayoshi</creatorcontrib><creatorcontrib>Zako, Masahiro</creatorcontrib><title>Characterization of a motif for specific binding to hyaluronan in chicken SPACR</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>The chicken sialoprotein associated with cones and rods (SPACR) binds to hyaluronan (HA) in the interphotoreceptor matrix space, but the motif for HA binding is still unknown. This study was conducted to determine the critical site required for specific binding to HA. Western blotting study showed that SPACR binds biotinylated HA, and this interaction was specifically inhibited by unlabeled HA. A series of GST fusion proteins covering whole SPACR was prepared, and reactivity with HA was individually screened to narrow down the region for the binding. Further, putative HA-binding motif found near the carboxyl-terminus of SPACR was mutated by site-directed mutagenesis to identify the critical binding site. Finally, we showed that native SPACR derived from retina similarly binds to HA-affinity column under both reducing and non-reducing conditions. These results revealed that the specific putative HA-binding motif is located near the carboxyl-terminus of chicken SPACR, and suggested that a structural integrity such as folded structure is not largely involved in the HA binding.</description><subject>Aminoacid receptors (glycine, glutamate, gaba)</subject><subject>Animals</subject><subject>Binding sites</subject><subject>Binding Sites - physiology</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Chickens</subject><subject>Cytoskeleton, cytoplasm. Intracellular movements</subject><subject>Extracellular Matrix Proteins - genetics</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Eye Proteins - genetics</subject><subject>Eye Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hyaluronan</subject><subject>hyaluronan-binding motif</subject><subject>Hyaluronic Acid - genetics</subject><subject>Hyaluronic Acid - metabolism</subject><subject>interphotoreceptor matrix</subject><subject>Molecular and cellular biology</subject><subject>Neurology</subject><subject>Protein Interaction Domains and Motifs - physiology</subject><subject>Proteins</subject><subject>Proteoglycans - genetics</subject><subject>Proteoglycans - metabolism</subject><subject>receptor for hyaluronan-mediated motility</subject><subject>Retina</subject><subject>Retina - metabolism</subject><subject>Sialoglycoproteins - genetics</subject><subject>Sialoglycoproteins - metabolism</subject><subject>sialoprotein associated with cones and rods</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE9vFCEYh4nR2G31KygxsbcZeRkYZg4emol_01hj65kwDHRZZ2ELM7Hrp5dxNzXxJBdIeH4vPx6EMJAS8nqzKYEJKBjwtqSENCXhrG7K-0do9XDxGK0IobSoCKMn6DSlDSFQsxqeohNoWF1XlK_QVbdWUenJRPdLTS54HCxWeBsmZ7ENEaed0c46jXvnB-dv8RTweq_GOQavPHYe67XTP4zH118vum_P0BOrxmSeH_czdPP-3U33sbi8-vCpu7gsNBNtUwgYhGkNZ9aqlvZCCNZooxltGbDG0ty_V-0AYBmHfhgyzsmgwALjQ99XZ-j8MHYXw91s0iS3LmkzjsqbMCdJSZNniiqDr_4BN2GOPlfLTM05VKLOUHOAdAwpRWPlLrqtinsJRC7C5UYuXuXiVS7C5R_h8j5HXxznz_3WDH-DR8MZeH0EVNJqtFF57dIDRwnPULtwbw_cTzea_X8XkJ-_dMsp518e8lYFqW5jfuP7NSVQEdJC_idUvwGLnaN7</recordid><startdate>200808</startdate><enddate>200808</enddate><creator>Zhao, Jinsong</creator><creator>Yoneda, Masahiko</creator><creator>Takeyama, Masayuki</creator><creator>Miyaishi, Osamu</creator><creator>Inoue, Yoko</creator><creator>Kataoka, Takuya</creator><creator>Ohno-Jinno, Akiko</creator><creator>Isogai, Zenzo</creator><creator>Kimata, Koji</creator><creator>Iwaki, Masayoshi</creator><creator>Zako, Masahiro</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>200808</creationdate><title>Characterization of a motif for specific binding to hyaluronan in chicken SPACR</title><author>Zhao, Jinsong ; Yoneda, Masahiko ; Takeyama, Masayuki ; Miyaishi, Osamu ; Inoue, Yoko ; Kataoka, Takuya ; Ohno-Jinno, Akiko ; Isogai, Zenzo ; Kimata, Koji ; Iwaki, Masayoshi ; Zako, Masahiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4798-71d7e9e54ffa92b77748cec4294148f2159ba9d11f451bdd1d750da1f145dbb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Aminoacid receptors (glycine, glutamate, gaba)</topic><topic>Animals</topic><topic>Binding sites</topic><topic>Binding Sites - physiology</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Chickens</topic><topic>Cytoskeleton, cytoplasm. Intracellular movements</topic><topic>Extracellular Matrix Proteins - genetics</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Eye Proteins - genetics</topic><topic>Eye Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hyaluronan</topic><topic>hyaluronan-binding motif</topic><topic>Hyaluronic Acid - genetics</topic><topic>Hyaluronic Acid - metabolism</topic><topic>interphotoreceptor matrix</topic><topic>Molecular and cellular biology</topic><topic>Neurology</topic><topic>Protein Interaction Domains and Motifs - physiology</topic><topic>Proteins</topic><topic>Proteoglycans - genetics</topic><topic>Proteoglycans - metabolism</topic><topic>receptor for hyaluronan-mediated motility</topic><topic>Retina</topic><topic>Retina - metabolism</topic><topic>Sialoglycoproteins - genetics</topic><topic>Sialoglycoproteins - metabolism</topic><topic>sialoprotein associated with cones and rods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Jinsong</creatorcontrib><creatorcontrib>Yoneda, Masahiko</creatorcontrib><creatorcontrib>Takeyama, Masayuki</creatorcontrib><creatorcontrib>Miyaishi, Osamu</creatorcontrib><creatorcontrib>Inoue, Yoko</creatorcontrib><creatorcontrib>Kataoka, Takuya</creatorcontrib><creatorcontrib>Ohno-Jinno, Akiko</creatorcontrib><creatorcontrib>Isogai, Zenzo</creatorcontrib><creatorcontrib>Kimata, Koji</creatorcontrib><creatorcontrib>Iwaki, Masayoshi</creatorcontrib><creatorcontrib>Zako, Masahiro</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Jinsong</au><au>Yoneda, Masahiko</au><au>Takeyama, Masayuki</au><au>Miyaishi, Osamu</au><au>Inoue, Yoko</au><au>Kataoka, Takuya</au><au>Ohno-Jinno, Akiko</au><au>Isogai, Zenzo</au><au>Kimata, Koji</au><au>Iwaki, Masayoshi</au><au>Zako, Masahiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a motif for specific binding to hyaluronan in chicken SPACR</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>2008-08</date><risdate>2008</risdate><volume>106</volume><issue>3</issue><spage>1117</spage><epage>1124</epage><pages>1117-1124</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>The chicken sialoprotein associated with cones and rods (SPACR) binds to hyaluronan (HA) in the interphotoreceptor matrix space, but the motif for HA binding is still unknown. This study was conducted to determine the critical site required for specific binding to HA. Western blotting study showed that SPACR binds biotinylated HA, and this interaction was specifically inhibited by unlabeled HA. A series of GST fusion proteins covering whole SPACR was prepared, and reactivity with HA was individually screened to narrow down the region for the binding. Further, putative HA-binding motif found near the carboxyl-terminus of SPACR was mutated by site-directed mutagenesis to identify the critical binding site. Finally, we showed that native SPACR derived from retina similarly binds to HA-affinity column under both reducing and non-reducing conditions. These results revealed that the specific putative HA-binding motif is located near the carboxyl-terminus of chicken SPACR, and suggested that a structural integrity such as folded structure is not largely involved in the HA binding.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18466325</pmid><doi>10.1111/j.1471-4159.2008.05468.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Aminoacid receptors (glycine, glutamate, gaba) Animals Binding sites Binding Sites - physiology Biochemistry Biological and medical sciences Cell receptors Cell structures and functions Chickens Cytoskeleton, cytoplasm. Intracellular movements Extracellular Matrix Proteins - genetics Extracellular Matrix Proteins - metabolism Eye Proteins - genetics Eye Proteins - metabolism Fundamental and applied biological sciences. Psychology hyaluronan hyaluronan-binding motif Hyaluronic Acid - genetics Hyaluronic Acid - metabolism interphotoreceptor matrix Molecular and cellular biology Neurology Protein Interaction Domains and Motifs - physiology Proteins Proteoglycans - genetics Proteoglycans - metabolism receptor for hyaluronan-mediated motility Retina Retina - metabolism Sialoglycoproteins - genetics Sialoglycoproteins - metabolism sialoprotein associated with cones and rods |
| title | Characterization of a motif for specific binding to hyaluronan in chicken SPACR |
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