The effect of blood flow restriction along with low-intensity exercise on cardiac structure and function in aging rat: Role of angiogenesis
Low-intensity aerobic training along with limbs blood flow restriction can improve mass and strength of skeletal muscle, but its effects on aging heart structure and performance is unidentified. We investigated the effects of this model of training on myocardial function, histology and angiogenesis...
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| Veröffentlicht in: | Life sciences (1973) 2018-09, Vol.209, p.202-209 |
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| creator | Naderi-boldaji, Vida Joukar, Siyavash Noorafshan, Ali Raji-amirhasani, Alireza Naderi-boldaji, Samaneh Bejeshk, Mohammad-abbas |
| description | Low-intensity aerobic training along with limbs blood flow restriction can improve mass and strength of skeletal muscle, but its effects on aging heart structure and performance is unidentified. We investigated the effects of this model of training on myocardial function, histology and angiogenesis in old male rats.
Animals randomly were divided into control (Ctl), sham-operated (Sh), limbs blood flow restriction (BFR), sham-operated plus 10 weeks low intensity treadmill exercise (Sh + Ex), and BFR plus exercise (BFR + Ex) groups. Finally, blood pressure, heart physiological and stereological parameters, myocardial oxygen consumption index and expression of vascular endothelial growth factor (VEGF) and its receptors (Flt-1 and kdr) were assessed.
BFR + Ex group had significantly lower heart rate (P |
| doi_str_mv | 10.1016/j.lfs.2018.08.015 |
| format | Article |
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Animals randomly were divided into control (Ctl), sham-operated (Sh), limbs blood flow restriction (BFR), sham-operated plus 10 weeks low intensity treadmill exercise (Sh + Ex), and BFR plus exercise (BFR + Ex) groups. Finally, blood pressure, heart physiological and stereological parameters, myocardial oxygen consumption index and expression of vascular endothelial growth factor (VEGF) and its receptors (Flt-1 and kdr) were assessed.
BFR + Ex group had significantly lower heart rate (P < 0.05 vs. Ctl and Sh groups), rate-pressure product (RPP) and left ventricular end diastolic pressure (P < 0.05 and P < 0.01 vs. untrained groups, respectively). BFR + Ex group also had greater +dp/dt max (P < 0.01) and −dp/dt max (P < 0.05) than untrained groups. A significant increase in volumes of left ventricle and myocytes (P < 0.05, vs. Ctl and Sham), ventricular hypertrophy index and capillaries length density (P < 0.05 vs. untrained groups) were observed in BFR + Ex group.
The level of VEGF and Flt-1 proteins and their mRNAs increased in the BFR + Ex group compared to Ctl, Sh and BFR (P < 0.01) and Sh + Ex (P < 0.05) groups. The kdr mRNA and its protein level were significantly higher in the BFR + Ex group.
Findings suggest that BFR plus exercise through improving the angiogenesis, physiological cardiac remodeling and oxygen demand/supply matching can promote cardiac performance in the elderly rats.
•Aging is associated with unwanted heart remodeling that leads to cardiac dysfunction.•Old rats were subjected to mild training and BFR for ten weeks.•Then, cardiac structure and function of aged rats were investigated.•BFR plus mild exercise improved the indices of cardiac function and O2 consumption.•This effect may mediate via improvement of angiogenesis and physiological hypertrophy.]]></description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2018.08.015</identifier><identifier>PMID: 30096385</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Aging ; Angiogenesis ; Animals ; Blood flow ; Blood flow restriction ; Blood pressure ; Capillaries ; Cardiac performance ; Cytotoxicity ; Diastolic pressure ; Geriatrics ; Growth factors ; Heart ; Heart - physiology ; Heart rate ; Hemodynamics ; Histology ; Hypertrophy ; Limbs ; Low intensity training ; Lower Extremity - blood supply ; Lymphocytes T ; Male ; mRNA ; Muscles ; Musculoskeletal system ; Myocytes ; Neovascularization, Physiologic ; Older people ; Oxygen consumption ; Oxygen demand ; Physical Conditioning, Animal ; Physical training ; Physiology ; Proteins ; Rats ; Rats, Wistar ; Receptors ; Regional Blood Flow ; Resistance Training ; Rodents ; Skeletal muscle ; Stereology ; Structure-function relationships ; Training ; Vascular endothelial growth factor ; VEGF receptors ; Ventricle</subject><ispartof>Life sciences (1973), 2018-09, Vol.209, p.202-209</ispartof><rights>2018</rights><rights>Copyright © 2018. Published by Elsevier Inc.</rights><rights>Copyright Elsevier BV Sep 15, 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-897a2adf241a1428d7523c8957565fa3eecc5cb51bb5798e3e75c8d4eadd9ea73</citedby><cites>FETCH-LOGICAL-c381t-897a2adf241a1428d7523c8957565fa3eecc5cb51bb5798e3e75c8d4eadd9ea73</cites><orcidid>0000-0002-9937-6985</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320518304612$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30096385$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naderi-boldaji, Vida</creatorcontrib><creatorcontrib>Joukar, Siyavash</creatorcontrib><creatorcontrib>Noorafshan, Ali</creatorcontrib><creatorcontrib>Raji-amirhasani, Alireza</creatorcontrib><creatorcontrib>Naderi-boldaji, Samaneh</creatorcontrib><creatorcontrib>Bejeshk, Mohammad-abbas</creatorcontrib><title>The effect of blood flow restriction along with low-intensity exercise on cardiac structure and function in aging rat: Role of angiogenesis</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description><![CDATA[Low-intensity aerobic training along with limbs blood flow restriction can improve mass and strength of skeletal muscle, but its effects on aging heart structure and performance is unidentified. We investigated the effects of this model of training on myocardial function, histology and angiogenesis in old male rats.
Animals randomly were divided into control (Ctl), sham-operated (Sh), limbs blood flow restriction (BFR), sham-operated plus 10 weeks low intensity treadmill exercise (Sh + Ex), and BFR plus exercise (BFR + Ex) groups. Finally, blood pressure, heart physiological and stereological parameters, myocardial oxygen consumption index and expression of vascular endothelial growth factor (VEGF) and its receptors (Flt-1 and kdr) were assessed.
BFR + Ex group had significantly lower heart rate (P < 0.05 vs. Ctl and Sh groups), rate-pressure product (RPP) and left ventricular end diastolic pressure (P < 0.05 and P < 0.01 vs. untrained groups, respectively). BFR + Ex group also had greater +dp/dt max (P < 0.01) and −dp/dt max (P < 0.05) than untrained groups. A significant increase in volumes of left ventricle and myocytes (P < 0.05, vs. Ctl and Sham), ventricular hypertrophy index and capillaries length density (P < 0.05 vs. untrained groups) were observed in BFR + Ex group.
The level of VEGF and Flt-1 proteins and their mRNAs increased in the BFR + Ex group compared to Ctl, Sh and BFR (P < 0.01) and Sh + Ex (P < 0.05) groups. The kdr mRNA and its protein level were significantly higher in the BFR + Ex group.
Findings suggest that BFR plus exercise through improving the angiogenesis, physiological cardiac remodeling and oxygen demand/supply matching can promote cardiac performance in the elderly rats.
•Aging is associated with unwanted heart remodeling that leads to cardiac dysfunction.•Old rats were subjected to mild training and BFR for ten weeks.•Then, cardiac structure and function of aged rats were investigated.•BFR plus mild exercise improved the indices of cardiac function and O2 consumption.•This effect may mediate via improvement of angiogenesis and physiological hypertrophy.]]></description><subject>Aging</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Blood flow</subject><subject>Blood flow restriction</subject><subject>Blood pressure</subject><subject>Capillaries</subject><subject>Cardiac performance</subject><subject>Cytotoxicity</subject><subject>Diastolic pressure</subject><subject>Geriatrics</subject><subject>Growth factors</subject><subject>Heart</subject><subject>Heart - physiology</subject><subject>Heart rate</subject><subject>Hemodynamics</subject><subject>Histology</subject><subject>Hypertrophy</subject><subject>Limbs</subject><subject>Low intensity training</subject><subject>Lower Extremity - blood supply</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>mRNA</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Myocytes</subject><subject>Neovascularization, Physiologic</subject><subject>Older people</subject><subject>Oxygen consumption</subject><subject>Oxygen demand</subject><subject>Physical Conditioning, Animal</subject><subject>Physical training</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors</subject><subject>Regional Blood Flow</subject><subject>Resistance Training</subject><subject>Rodents</subject><subject>Skeletal muscle</subject><subject>Stereology</subject><subject>Structure-function relationships</subject><subject>Training</subject><subject>Vascular endothelial growth factor</subject><subject>VEGF receptors</subject><subject>Ventricle</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1rVDEUhoNY7Fj9AW4k4MbNneYkk5tcXUnxCwqC1HXITU6mGe4kNbnX2t_QP22GqS5cFA5kked98vES8grYGhj057v1FOqaM9Br1gbkE7ICrYaO9QKekhVjfNMJzuQpeV7rjjEmpRLPyKlgbOiFlityf3WNFENAN9Mc6Djl7GmY8i0tWOcS3RxzonbKaUtv43xN21YX04ypxvmO4m8sLlakDXK2-GgdbbHFzUtBalNzLenoiE2zjU1T7PyOfs8THg60aRvzFhPWWF-Qk2Cnii8f1jPy49PHq4sv3eW3z18vPlx2TmiYOz0oy60PfAMWNlx7JblwepBK9jJYgeicdKOEcZRq0ChQSaf9Bq33A1olzsjbo_em5J9Le6bZx-pwmmzCvFTDmVZy6EH2DX3zH7rLS0ntdoYDaKFADbxRcKRcybUWDOamxL0tdwaYOTRldqY1ZQ5NGdYGZMu8fjAv4x79v8Tfahrw_ghg-4pfEYupLmJy6GNpbRmf4yP6Py_gpfw</recordid><startdate>20180915</startdate><enddate>20180915</enddate><creator>Naderi-boldaji, Vida</creator><creator>Joukar, Siyavash</creator><creator>Noorafshan, Ali</creator><creator>Raji-amirhasani, Alireza</creator><creator>Naderi-boldaji, Samaneh</creator><creator>Bejeshk, Mohammad-abbas</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9937-6985</orcidid></search><sort><creationdate>20180915</creationdate><title>The effect of blood flow restriction along with low-intensity exercise on cardiac structure and function in aging rat: Role of angiogenesis</title><author>Naderi-boldaji, Vida ; Joukar, Siyavash ; Noorafshan, Ali ; Raji-amirhasani, Alireza ; Naderi-boldaji, Samaneh ; Bejeshk, Mohammad-abbas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-897a2adf241a1428d7523c8957565fa3eecc5cb51bb5798e3e75c8d4eadd9ea73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Aging</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Blood flow</topic><topic>Blood flow restriction</topic><topic>Blood pressure</topic><topic>Capillaries</topic><topic>Cardiac performance</topic><topic>Cytotoxicity</topic><topic>Diastolic pressure</topic><topic>Geriatrics</topic><topic>Growth factors</topic><topic>Heart</topic><topic>Heart - physiology</topic><topic>Heart rate</topic><topic>Hemodynamics</topic><topic>Histology</topic><topic>Hypertrophy</topic><topic>Limbs</topic><topic>Low intensity training</topic><topic>Lower Extremity - blood supply</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>mRNA</topic><topic>Muscles</topic><topic>Musculoskeletal system</topic><topic>Myocytes</topic><topic>Neovascularization, Physiologic</topic><topic>Older people</topic><topic>Oxygen consumption</topic><topic>Oxygen demand</topic><topic>Physical Conditioning, Animal</topic><topic>Physical training</topic><topic>Physiology</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors</topic><topic>Regional Blood Flow</topic><topic>Resistance Training</topic><topic>Rodents</topic><topic>Skeletal muscle</topic><topic>Stereology</topic><topic>Structure-function relationships</topic><topic>Training</topic><topic>Vascular endothelial growth factor</topic><topic>VEGF receptors</topic><topic>Ventricle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naderi-boldaji, Vida</creatorcontrib><creatorcontrib>Joukar, Siyavash</creatorcontrib><creatorcontrib>Noorafshan, Ali</creatorcontrib><creatorcontrib>Raji-amirhasani, Alireza</creatorcontrib><creatorcontrib>Naderi-boldaji, Samaneh</creatorcontrib><creatorcontrib>Bejeshk, Mohammad-abbas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naderi-boldaji, Vida</au><au>Joukar, Siyavash</au><au>Noorafshan, Ali</au><au>Raji-amirhasani, Alireza</au><au>Naderi-boldaji, Samaneh</au><au>Bejeshk, Mohammad-abbas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of blood flow restriction along with low-intensity exercise on cardiac structure and function in aging rat: Role of angiogenesis</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2018-09-15</date><risdate>2018</risdate><volume>209</volume><spage>202</spage><epage>209</epage><pages>202-209</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract><![CDATA[Low-intensity aerobic training along with limbs blood flow restriction can improve mass and strength of skeletal muscle, but its effects on aging heart structure and performance is unidentified. We investigated the effects of this model of training on myocardial function, histology and angiogenesis in old male rats.
Animals randomly were divided into control (Ctl), sham-operated (Sh), limbs blood flow restriction (BFR), sham-operated plus 10 weeks low intensity treadmill exercise (Sh + Ex), and BFR plus exercise (BFR + Ex) groups. Finally, blood pressure, heart physiological and stereological parameters, myocardial oxygen consumption index and expression of vascular endothelial growth factor (VEGF) and its receptors (Flt-1 and kdr) were assessed.
BFR + Ex group had significantly lower heart rate (P < 0.05 vs. Ctl and Sh groups), rate-pressure product (RPP) and left ventricular end diastolic pressure (P < 0.05 and P < 0.01 vs. untrained groups, respectively). BFR + Ex group also had greater +dp/dt max (P < 0.01) and −dp/dt max (P < 0.05) than untrained groups. A significant increase in volumes of left ventricle and myocytes (P < 0.05, vs. Ctl and Sham), ventricular hypertrophy index and capillaries length density (P < 0.05 vs. untrained groups) were observed in BFR + Ex group.
The level of VEGF and Flt-1 proteins and their mRNAs increased in the BFR + Ex group compared to Ctl, Sh and BFR (P < 0.01) and Sh + Ex (P < 0.05) groups. The kdr mRNA and its protein level were significantly higher in the BFR + Ex group.
Findings suggest that BFR plus exercise through improving the angiogenesis, physiological cardiac remodeling and oxygen demand/supply matching can promote cardiac performance in the elderly rats.
•Aging is associated with unwanted heart remodeling that leads to cardiac dysfunction.•Old rats were subjected to mild training and BFR for ten weeks.•Then, cardiac structure and function of aged rats were investigated.•BFR plus mild exercise improved the indices of cardiac function and O2 consumption.•This effect may mediate via improvement of angiogenesis and physiological hypertrophy.]]></abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>30096385</pmid><doi>10.1016/j.lfs.2018.08.015</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-9937-6985</orcidid></addata></record> |
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| subjects | Aging Angiogenesis Animals Blood flow Blood flow restriction Blood pressure Capillaries Cardiac performance Cytotoxicity Diastolic pressure Geriatrics Growth factors Heart Heart - physiology Heart rate Hemodynamics Histology Hypertrophy Limbs Low intensity training Lower Extremity - blood supply Lymphocytes T Male mRNA Muscles Musculoskeletal system Myocytes Neovascularization, Physiologic Older people Oxygen consumption Oxygen demand Physical Conditioning, Animal Physical training Physiology Proteins Rats Rats, Wistar Receptors Regional Blood Flow Resistance Training Rodents Skeletal muscle Stereology Structure-function relationships Training Vascular endothelial growth factor VEGF receptors Ventricle |
| title | The effect of blood flow restriction along with low-intensity exercise on cardiac structure and function in aging rat: Role of angiogenesis |
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