Prevalence and antifungal susceptibility profiles of Candida glabrata, Candida parapsilosis and their close-related species in oral candidiasis
•C. glabrata and C. parapsilosis isolates from oral candidiasis were analysed.•PCR-RFLP of SADH gene identified isolates of C. metapsilosis and C. orthopsilosis.•C. glabrata was mainly isolated in mixed cultures and C. parapsilosis in pure cultures.•Nystatin and fluconazole were the most active anti...
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| Veröffentlicht in: | Archives of oral biology 2018-11, Vol.95, p.100-107 |
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| creator | Miranda-Cadena, Katherine Marcos-Arias, Cristina Mateo, Estibaliz Aguirre, José Manuel Quindós, Guillermo Eraso, Elena |
| description | •C. glabrata and C. parapsilosis isolates from oral candidiasis were analysed.•PCR-RFLP of SADH gene identified isolates of C. metapsilosis and C. orthopsilosis.•C. glabrata was mainly isolated in mixed cultures and C. parapsilosis in pure cultures.•Nystatin and fluconazole were the most active antifungal agents.•Azole cross resistance was detected both in C. parapsilosis and C. glabrata isolates.
To evaluate the importance of Candida glabrata, Candida parapsilosis and their close-related species, Candida bracarensis, Candida nivariensis, Candida metapsilosis and Candida orthopsilosis in patients with oral candidiasis and, to determine the in vitro activities of antifungal drugs currently used for the treatment.
One hundred fourteen isolates of C. glabrata and 97 of C. parapsilosis, previously identified by conventional mycological methods, were analysed by molecular techniques. In vitro antifungal susceptibility to fluconazole, itraconazole, miconazole, and nystatin was evaluated by CLSI M44-A2 disk diffusion test, and by CLSI M27-A3 microdilution for fluconazole.
All C. glabrata isolates were identified as C. glabrata sensu stricto, 93 out of 97 C. parapsilosis isolates as C. parapsilosis sensu stricto, three as C. orthopsilosis and one as C. metapsilosis. Candida glabrata was mainly isolated in mixed cultures but C. parapsilosis complex was more frequent in pure culture. Candida metapsilosis and C. orthopsilosis were isolated as pure culture and both species were susceptible to all antifungal agents tested. Most C. glabrata isolates were susceptible to miconazole and nystatin, but resistant to fluconazole and itraconazole. Azole cross resistance was also observed. Candida parapsilosis isolates were susceptible to fluconazole although azole cross resistance to miconazole and itraconazole was observed.
This study highlights the importance of accurate identification and antifungal susceptibility testing of oral Candida isolates in order to have an in-depth understanding of the role of C. glabrata and C. parapsilosis in oral candidiasis. |
| doi_str_mv | 10.1016/j.archoralbio.2018.07.017 |
| format | Article |
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To evaluate the importance of Candida glabrata, Candida parapsilosis and their close-related species, Candida bracarensis, Candida nivariensis, Candida metapsilosis and Candida orthopsilosis in patients with oral candidiasis and, to determine the in vitro activities of antifungal drugs currently used for the treatment.
One hundred fourteen isolates of C. glabrata and 97 of C. parapsilosis, previously identified by conventional mycological methods, were analysed by molecular techniques. In vitro antifungal susceptibility to fluconazole, itraconazole, miconazole, and nystatin was evaluated by CLSI M44-A2 disk diffusion test, and by CLSI M27-A3 microdilution for fluconazole.
All C. glabrata isolates were identified as C. glabrata sensu stricto, 93 out of 97 C. parapsilosis isolates as C. parapsilosis sensu stricto, three as C. orthopsilosis and one as C. metapsilosis. Candida glabrata was mainly isolated in mixed cultures but C. parapsilosis complex was more frequent in pure culture. Candida metapsilosis and C. orthopsilosis were isolated as pure culture and both species were susceptible to all antifungal agents tested. Most C. glabrata isolates were susceptible to miconazole and nystatin, but resistant to fluconazole and itraconazole. Azole cross resistance was also observed. Candida parapsilosis isolates were susceptible to fluconazole although azole cross resistance to miconazole and itraconazole was observed.
This study highlights the importance of accurate identification and antifungal susceptibility testing of oral Candida isolates in order to have an in-depth understanding of the role of C. glabrata and C. parapsilosis in oral candidiasis.</description><identifier>ISSN: 0003-9969</identifier><identifier>EISSN: 1879-1506</identifier><identifier>DOI: 10.1016/j.archoralbio.2018.07.017</identifier><identifier>PMID: 30096698</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antifungal Agents - pharmacology ; Antifungal susceptibility ; Candida glabrata ; Candida glabrata - drug effects ; Candida glabrata - isolation & purification ; Candida parapsilosis - drug effects ; Candida parapsilosis - isolation & purification ; Candida parapsilosis complex ; Candidiasis, Oral - drug therapy ; Candidiasis, Oral - microbiology ; Dentistry ; Drug Resistance, Fungal ; Female ; Humans ; Male ; Miconazole - pharmacology ; Middle Aged ; Nystatin - pharmacology ; Oral candidiasis ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Prevalence</subject><ispartof>Archives of oral biology, 2018-11, Vol.95, p.100-107</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-ce31f73b1760b2fa119e341d417d6f1422f1c7ab311f0267c9380c478623853b3</citedby><cites>FETCH-LOGICAL-c377t-ce31f73b1760b2fa119e341d417d6f1422f1c7ab311f0267c9380c478623853b3</cites><orcidid>0000-0002-0924-3696 ; 0000-0002-9043-9265</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.archoralbio.2018.07.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30096698$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miranda-Cadena, Katherine</creatorcontrib><creatorcontrib>Marcos-Arias, Cristina</creatorcontrib><creatorcontrib>Mateo, Estibaliz</creatorcontrib><creatorcontrib>Aguirre, José Manuel</creatorcontrib><creatorcontrib>Quindós, Guillermo</creatorcontrib><creatorcontrib>Eraso, Elena</creatorcontrib><title>Prevalence and antifungal susceptibility profiles of Candida glabrata, Candida parapsilosis and their close-related species in oral candidiasis</title><title>Archives of oral biology</title><addtitle>Arch Oral Biol</addtitle><description>•C. glabrata and C. parapsilosis isolates from oral candidiasis were analysed.•PCR-RFLP of SADH gene identified isolates of C. metapsilosis and C. orthopsilosis.•C. glabrata was mainly isolated in mixed cultures and C. parapsilosis in pure cultures.•Nystatin and fluconazole were the most active antifungal agents.•Azole cross resistance was detected both in C. parapsilosis and C. glabrata isolates.
To evaluate the importance of Candida glabrata, Candida parapsilosis and their close-related species, Candida bracarensis, Candida nivariensis, Candida metapsilosis and Candida orthopsilosis in patients with oral candidiasis and, to determine the in vitro activities of antifungal drugs currently used for the treatment.
One hundred fourteen isolates of C. glabrata and 97 of C. parapsilosis, previously identified by conventional mycological methods, were analysed by molecular techniques. In vitro antifungal susceptibility to fluconazole, itraconazole, miconazole, and nystatin was evaluated by CLSI M44-A2 disk diffusion test, and by CLSI M27-A3 microdilution for fluconazole.
All C. glabrata isolates were identified as C. glabrata sensu stricto, 93 out of 97 C. parapsilosis isolates as C. parapsilosis sensu stricto, three as C. orthopsilosis and one as C. metapsilosis. Candida glabrata was mainly isolated in mixed cultures but C. parapsilosis complex was more frequent in pure culture. Candida metapsilosis and C. orthopsilosis were isolated as pure culture and both species were susceptible to all antifungal agents tested. Most C. glabrata isolates were susceptible to miconazole and nystatin, but resistant to fluconazole and itraconazole. Azole cross resistance was also observed. Candida parapsilosis isolates were susceptible to fluconazole although azole cross resistance to miconazole and itraconazole was observed.
This study highlights the importance of accurate identification and antifungal susceptibility testing of oral Candida isolates in order to have an in-depth understanding of the role of C. glabrata and C. parapsilosis in oral candidiasis.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antifungal Agents - pharmacology</subject><subject>Antifungal susceptibility</subject><subject>Candida glabrata</subject><subject>Candida glabrata - drug effects</subject><subject>Candida glabrata - isolation & purification</subject><subject>Candida parapsilosis - drug effects</subject><subject>Candida parapsilosis - isolation & purification</subject><subject>Candida parapsilosis complex</subject><subject>Candidiasis, Oral - drug therapy</subject><subject>Candidiasis, Oral - microbiology</subject><subject>Dentistry</subject><subject>Drug Resistance, Fungal</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Miconazole - pharmacology</subject><subject>Middle Aged</subject><subject>Nystatin - pharmacology</subject><subject>Oral candidiasis</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Prevalence</subject><issn>0003-9969</issn><issn>1879-1506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhFZDZsSDBN07seIlG_EmVYAFry3Gu2zvyJMFOKvUpeGU8nVKxZGFZvjrnXB1_jL0BUYMA9f5Qu-Rv5uTiQHPdCOhroWsB-gnbQa9NBZ1QT9lOCCErY5S5YC9yPpRnpxQ8ZxdSCKOU6Xfs9_eEty7i5JG7aSxnpbBN1y7yvGWPy0oDRVrv-JLmQBEznwPfFymNjl9HNyS3unePk8Ult2SKc6Z8H7jeICXuywCrhNGtOPK8oKeSRBM_leD-3kyueF6yZ8HFjK8e7kv289PHH_sv1dW3z1_3H64qL7VeK48SgpYDaCWGJjgAg7KFsQU9qgBt0wTw2g0SIIhGaW9kL3yre9XIvpODvGRvz7ml1q8N82qPVOrG6Cact2wb0evOdI1pi9ScpT7NOScMdkl0dOnOgrAnHvZg_-FhTzys0LbwKN7XD2u24Yjjo_MvgCLYnwVYyt4SJpvL1xQaIyX0qx1n-o81fwD2qqSS</recordid><startdate>201811</startdate><enddate>201811</enddate><creator>Miranda-Cadena, Katherine</creator><creator>Marcos-Arias, Cristina</creator><creator>Mateo, Estibaliz</creator><creator>Aguirre, José Manuel</creator><creator>Quindós, Guillermo</creator><creator>Eraso, Elena</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0924-3696</orcidid><orcidid>https://orcid.org/0000-0002-9043-9265</orcidid></search><sort><creationdate>201811</creationdate><title>Prevalence and antifungal susceptibility profiles of Candida glabrata, Candida parapsilosis and their close-related species in oral candidiasis</title><author>Miranda-Cadena, Katherine ; Marcos-Arias, Cristina ; Mateo, Estibaliz ; Aguirre, José Manuel ; Quindós, Guillermo ; Eraso, Elena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-ce31f73b1760b2fa119e341d417d6f1422f1c7ab311f0267c9380c478623853b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antifungal Agents - pharmacology</topic><topic>Antifungal susceptibility</topic><topic>Candida glabrata</topic><topic>Candida glabrata - drug effects</topic><topic>Candida glabrata - isolation & purification</topic><topic>Candida parapsilosis - drug effects</topic><topic>Candida parapsilosis - isolation & purification</topic><topic>Candida parapsilosis complex</topic><topic>Candidiasis, Oral - drug therapy</topic><topic>Candidiasis, Oral - microbiology</topic><topic>Dentistry</topic><topic>Drug Resistance, Fungal</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Miconazole - pharmacology</topic><topic>Middle Aged</topic><topic>Nystatin - pharmacology</topic><topic>Oral candidiasis</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Prevalence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miranda-Cadena, Katherine</creatorcontrib><creatorcontrib>Marcos-Arias, Cristina</creatorcontrib><creatorcontrib>Mateo, Estibaliz</creatorcontrib><creatorcontrib>Aguirre, José Manuel</creatorcontrib><creatorcontrib>Quindós, Guillermo</creatorcontrib><creatorcontrib>Eraso, Elena</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of oral biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miranda-Cadena, Katherine</au><au>Marcos-Arias, Cristina</au><au>Mateo, Estibaliz</au><au>Aguirre, José Manuel</au><au>Quindós, Guillermo</au><au>Eraso, Elena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and antifungal susceptibility profiles of Candida glabrata, Candida parapsilosis and their close-related species in oral candidiasis</atitle><jtitle>Archives of oral biology</jtitle><addtitle>Arch Oral Biol</addtitle><date>2018-11</date><risdate>2018</risdate><volume>95</volume><spage>100</spage><epage>107</epage><pages>100-107</pages><issn>0003-9969</issn><eissn>1879-1506</eissn><abstract>•C. glabrata and C. parapsilosis isolates from oral candidiasis were analysed.•PCR-RFLP of SADH gene identified isolates of C. metapsilosis and C. orthopsilosis.•C. glabrata was mainly isolated in mixed cultures and C. parapsilosis in pure cultures.•Nystatin and fluconazole were the most active antifungal agents.•Azole cross resistance was detected both in C. parapsilosis and C. glabrata isolates.
To evaluate the importance of Candida glabrata, Candida parapsilosis and their close-related species, Candida bracarensis, Candida nivariensis, Candida metapsilosis and Candida orthopsilosis in patients with oral candidiasis and, to determine the in vitro activities of antifungal drugs currently used for the treatment.
One hundred fourteen isolates of C. glabrata and 97 of C. parapsilosis, previously identified by conventional mycological methods, were analysed by molecular techniques. In vitro antifungal susceptibility to fluconazole, itraconazole, miconazole, and nystatin was evaluated by CLSI M44-A2 disk diffusion test, and by CLSI M27-A3 microdilution for fluconazole.
All C. glabrata isolates were identified as C. glabrata sensu stricto, 93 out of 97 C. parapsilosis isolates as C. parapsilosis sensu stricto, three as C. orthopsilosis and one as C. metapsilosis. Candida glabrata was mainly isolated in mixed cultures but C. parapsilosis complex was more frequent in pure culture. Candida metapsilosis and C. orthopsilosis were isolated as pure culture and both species were susceptible to all antifungal agents tested. Most C. glabrata isolates were susceptible to miconazole and nystatin, but resistant to fluconazole and itraconazole. Azole cross resistance was also observed. Candida parapsilosis isolates were susceptible to fluconazole although azole cross resistance to miconazole and itraconazole was observed.
This study highlights the importance of accurate identification and antifungal susceptibility testing of oral Candida isolates in order to have an in-depth understanding of the role of C. glabrata and C. parapsilosis in oral candidiasis.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>30096698</pmid><doi>10.1016/j.archoralbio.2018.07.017</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-0924-3696</orcidid><orcidid>https://orcid.org/0000-0002-9043-9265</orcidid></addata></record> |
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| ispartof | Archives of oral biology, 2018-11, Vol.95, p.100-107 |
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| subjects | Adolescent Adult Aged Aged, 80 and over Antifungal Agents - pharmacology Antifungal susceptibility Candida glabrata Candida glabrata - drug effects Candida glabrata - isolation & purification Candida parapsilosis - drug effects Candida parapsilosis - isolation & purification Candida parapsilosis complex Candidiasis, Oral - drug therapy Candidiasis, Oral - microbiology Dentistry Drug Resistance, Fungal Female Humans Male Miconazole - pharmacology Middle Aged Nystatin - pharmacology Oral candidiasis Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Prevalence |
| title | Prevalence and antifungal susceptibility profiles of Candida glabrata, Candida parapsilosis and their close-related species in oral candidiasis |
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