Validation of micronuclei frequency in peripheral blood lymphocytes as early cancer risk biomarker in a nested case–control study
Aim of this work was to assess the predictive value of micronuclei (MN) frequency in peripheral blood lymphocytes (PBL) for the risk of cancer death in disease-free individuals. Blood samples from 1650 subjects selected from the general population of Pisa, Italy, were collected between June 1991 and...
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| Veröffentlicht in: | Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis 2008-03, Vol.639 (1), p.27-34 |
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| creator | Murgia, Elena Ballardin, Michela Bonassi, Stefano Rossi, Anna Maria Barale, Roberto |
| description | Aim of this work was to assess the predictive value of micronuclei (MN) frequency in peripheral blood lymphocytes (PBL) for the risk of cancer death in disease-free individuals. Blood samples from 1650 subjects selected from the general population of Pisa, Italy, were collected between June 1991 and November 1993. The follow-up until January 2005 recorded a total of 111 deaths (52 for cancer). MN frequency was assessed for 49 cancer cases and 101 matched controls. A significantly higher MN frequency was found in cancer cases (4.7
±
3.4
MN/1000 BN cells) versus controls (1.5
±
1.7;
p
<
0.0001). Donors were stratified in two classes and multivariate logistic regression analysis confirmed that individuals with high MN frequency (>2.5
MN/1000 BN cells) had a significantly increased risk of cancer death (OR
=
10.7; 95% CI
=
4.6–24.9;
p
<
0.0001) when compared to individuals with low MN frequency (≤2.5
MN/1000 BN cells). Ageing was associated with a 6% increased risk per year (
p
=
0.03). No influence of other potential confounders (gender, occupation, smoking and drinking habits) was observed. Finally, subjects with a higher MN frequency showed a higher MR for CVD (Logrank test,
p
=
0.001).
These findings provide strong evidence that MN frequency assessed in PBL of disease-free subjects is a good predictor of cancer death risk, evaluated by a nested case–control study performed 14 years after the original recruitment. |
| doi_str_mv | 10.1016/j.mrfmmm.2007.10.010 |
| format | Article |
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±
3.4
MN/1000 BN cells) versus controls (1.5
±
1.7;
p
<
0.0001). Donors were stratified in two classes and multivariate logistic regression analysis confirmed that individuals with high MN frequency (>2.5
MN/1000 BN cells) had a significantly increased risk of cancer death (OR
=
10.7; 95% CI
=
4.6–24.9;
p
<
0.0001) when compared to individuals with low MN frequency (≤2.5
MN/1000 BN cells). Ageing was associated with a 6% increased risk per year (
p
=
0.03). No influence of other potential confounders (gender, occupation, smoking and drinking habits) was observed. Finally, subjects with a higher MN frequency showed a higher MR for CVD (Logrank test,
p
=
0.001).
These findings provide strong evidence that MN frequency assessed in PBL of disease-free subjects is a good predictor of cancer death risk, evaluated by a nested case–control study performed 14 years after the original recruitment.</description><identifier>ISSN: 0027-5107</identifier><identifier>ISSN: 1386-1964</identifier><identifier>EISSN: 1873-135X</identifier><identifier>EISSN: 0027-5107</identifier><identifier>DOI: 10.1016/j.mrfmmm.2007.10.010</identifier><identifier>PMID: 18155071</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Aged ; Biological markers ; Cancer risk ; Case-Control Studies ; Case–control study ; Cause of Death ; Early Diagnosis ; Female ; Follow-Up Studies ; Gene Frequency ; Humans ; Italy ; Lymphocytes - metabolism ; Male ; Micronuclei, Chromosome-Defective ; Micronucleus test ; Middle Aged ; Neoplasms - blood ; Neoplasms - diagnosis ; Neoplasms - genetics ; Neoplasms - mortality ; Prognosis ; Sensitivity and Specificity ; Survival Analysis ; Validation</subject><ispartof>Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2008-03, Vol.639 (1), p.27-34</ispartof><rights>2007 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-8b0cfd7033ac1a4ad42bc69ce5ee26790b6b70d1ce6cbc0bef27012c278a5b173</citedby><cites>FETCH-LOGICAL-c457t-8b0cfd7033ac1a4ad42bc69ce5ee26790b6b70d1ce6cbc0bef27012c278a5b173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18155071$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murgia, Elena</creatorcontrib><creatorcontrib>Ballardin, Michela</creatorcontrib><creatorcontrib>Bonassi, Stefano</creatorcontrib><creatorcontrib>Rossi, Anna Maria</creatorcontrib><creatorcontrib>Barale, Roberto</creatorcontrib><title>Validation of micronuclei frequency in peripheral blood lymphocytes as early cancer risk biomarker in a nested case–control study</title><title>Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis</title><addtitle>Mutat Res</addtitle><description>Aim of this work was to assess the predictive value of micronuclei (MN) frequency in peripheral blood lymphocytes (PBL) for the risk of cancer death in disease-free individuals. Blood samples from 1650 subjects selected from the general population of Pisa, Italy, were collected between June 1991 and November 1993. The follow-up until January 2005 recorded a total of 111 deaths (52 for cancer). MN frequency was assessed for 49 cancer cases and 101 matched controls. A significantly higher MN frequency was found in cancer cases (4.7
±
3.4
MN/1000 BN cells) versus controls (1.5
±
1.7;
p
<
0.0001). Donors were stratified in two classes and multivariate logistic regression analysis confirmed that individuals with high MN frequency (>2.5
MN/1000 BN cells) had a significantly increased risk of cancer death (OR
=
10.7; 95% CI
=
4.6–24.9;
p
<
0.0001) when compared to individuals with low MN frequency (≤2.5
MN/1000 BN cells). Ageing was associated with a 6% increased risk per year (
p
=
0.03). No influence of other potential confounders (gender, occupation, smoking and drinking habits) was observed. Finally, subjects with a higher MN frequency showed a higher MR for CVD (Logrank test,
p
=
0.001).
These findings provide strong evidence that MN frequency assessed in PBL of disease-free subjects is a good predictor of cancer death risk, evaluated by a nested case–control study performed 14 years after the original recruitment.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological markers</subject><subject>Cancer risk</subject><subject>Case-Control Studies</subject><subject>Case–control study</subject><subject>Cause of Death</subject><subject>Early Diagnosis</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Gene Frequency</subject><subject>Humans</subject><subject>Italy</subject><subject>Lymphocytes - metabolism</subject><subject>Male</subject><subject>Micronuclei, Chromosome-Defective</subject><subject>Micronucleus test</subject><subject>Middle Aged</subject><subject>Neoplasms - blood</subject><subject>Neoplasms - diagnosis</subject><subject>Neoplasms - genetics</subject><subject>Neoplasms - mortality</subject><subject>Prognosis</subject><subject>Sensitivity and Specificity</subject><subject>Survival Analysis</subject><subject>Validation</subject><issn>0027-5107</issn><issn>1386-1964</issn><issn>1873-135X</issn><issn>0027-5107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2KFDEQx4Mo7rj6BiI5eeuxkv5I90WQZXWFBS8q3kI-qtnMJp026V7om-Aj-IY-iRlmwJueQhW_f4WqHyEvGewZsO7NYR_SGELYcwBRWntg8IjsWC_qitXtt8dkB8BF1TIQF-RZzgcAaIZueEouWM_aFgTbkZ9flXdWLS5ONI40OJPitBqPjo4Jv684mY26ic6Y3HyHSXmqfYyW-i3Md9FsC2aqMkWV_EaNmgwmmly-p9rFoNJ9KUtc0QnzgrYQGX__-GXitKToaV5Wuz0nT0blM744v5fky_vrz1c31e2nDx-v3t1WpmnFUvUazGgF1LUyTDXKNlybbjDYIvJODKA7LcAyg53RBjSOXADjhotetZqJ-pK8Ps2dUyyb5UUGlw16ryaMa5ZlNB9Ey_8Lcui7VjBWwOYElqvlnHCUc3Jl600ykEdL8iBPluTR0rFbLJXYq_P8VQe0f0NnLQV4ewKwnOPBYZLZuKICrUtoFmmj-_cPfwBSQqni</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Murgia, Elena</creator><creator>Ballardin, Michela</creator><creator>Bonassi, Stefano</creator><creator>Rossi, Anna Maria</creator><creator>Barale, Roberto</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U7</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080301</creationdate><title>Validation of micronuclei frequency in peripheral blood lymphocytes as early cancer risk biomarker in a nested case–control study</title><author>Murgia, Elena ; Ballardin, Michela ; Bonassi, Stefano ; Rossi, Anna Maria ; Barale, Roberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-8b0cfd7033ac1a4ad42bc69ce5ee26790b6b70d1ce6cbc0bef27012c278a5b173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological markers</topic><topic>Cancer risk</topic><topic>Case-Control Studies</topic><topic>Case–control study</topic><topic>Cause of Death</topic><topic>Early Diagnosis</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Gene Frequency</topic><topic>Humans</topic><topic>Italy</topic><topic>Lymphocytes - metabolism</topic><topic>Male</topic><topic>Micronuclei, Chromosome-Defective</topic><topic>Micronucleus test</topic><topic>Middle Aged</topic><topic>Neoplasms - blood</topic><topic>Neoplasms - diagnosis</topic><topic>Neoplasms - genetics</topic><topic>Neoplasms - mortality</topic><topic>Prognosis</topic><topic>Sensitivity and Specificity</topic><topic>Survival Analysis</topic><topic>Validation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murgia, Elena</creatorcontrib><creatorcontrib>Ballardin, Michela</creatorcontrib><creatorcontrib>Bonassi, Stefano</creatorcontrib><creatorcontrib>Rossi, Anna Maria</creatorcontrib><creatorcontrib>Barale, Roberto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murgia, Elena</au><au>Ballardin, Michela</au><au>Bonassi, Stefano</au><au>Rossi, Anna Maria</au><au>Barale, Roberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validation of micronuclei frequency in peripheral blood lymphocytes as early cancer risk biomarker in a nested case–control study</atitle><jtitle>Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis</jtitle><addtitle>Mutat Res</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>639</volume><issue>1</issue><spage>27</spage><epage>34</epage><pages>27-34</pages><issn>0027-5107</issn><issn>1386-1964</issn><eissn>1873-135X</eissn><eissn>0027-5107</eissn><abstract>Aim of this work was to assess the predictive value of micronuclei (MN) frequency in peripheral blood lymphocytes (PBL) for the risk of cancer death in disease-free individuals. Blood samples from 1650 subjects selected from the general population of Pisa, Italy, were collected between June 1991 and November 1993. The follow-up until January 2005 recorded a total of 111 deaths (52 for cancer). MN frequency was assessed for 49 cancer cases and 101 matched controls. A significantly higher MN frequency was found in cancer cases (4.7
±
3.4
MN/1000 BN cells) versus controls (1.5
±
1.7;
p
<
0.0001). Donors were stratified in two classes and multivariate logistic regression analysis confirmed that individuals with high MN frequency (>2.5
MN/1000 BN cells) had a significantly increased risk of cancer death (OR
=
10.7; 95% CI
=
4.6–24.9;
p
<
0.0001) when compared to individuals with low MN frequency (≤2.5
MN/1000 BN cells). Ageing was associated with a 6% increased risk per year (
p
=
0.03). No influence of other potential confounders (gender, occupation, smoking and drinking habits) was observed. Finally, subjects with a higher MN frequency showed a higher MR for CVD (Logrank test,
p
=
0.001).
These findings provide strong evidence that MN frequency assessed in PBL of disease-free subjects is a good predictor of cancer death risk, evaluated by a nested case–control study performed 14 years after the original recruitment.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>18155071</pmid><doi>10.1016/j.mrfmmm.2007.10.010</doi><tpages>8</tpages></addata></record> |
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| ispartof | Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2008-03, Vol.639 (1), p.27-34 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adult Aged Biological markers Cancer risk Case-Control Studies Case–control study Cause of Death Early Diagnosis Female Follow-Up Studies Gene Frequency Humans Italy Lymphocytes - metabolism Male Micronuclei, Chromosome-Defective Micronucleus test Middle Aged Neoplasms - blood Neoplasms - diagnosis Neoplasms - genetics Neoplasms - mortality Prognosis Sensitivity and Specificity Survival Analysis Validation |
| title | Validation of micronuclei frequency in peripheral blood lymphocytes as early cancer risk biomarker in a nested case–control study |
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