Cytochrome P450 monooxygenase-mediated permethrin resistance confers limited and larval specific cross-resistance in the southern house mosquito, Culex pipiens quinquefasciatus
The cytochrome P450-dependent monooxygenases (P450s) are an important enzymatic system that metabolizes xenobiotics (e.g., pesticides), as well as endogenous compounds (e.g., hormones). P450-mediated metabolism can result in detoxification of insecticides such as pyrethroids, or can be involved in t...
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| Veröffentlicht in: | Pesticide biochemistry and physiology 2007-11, Vol.89 (3), p.175-184 |
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| creator | Hardstone, Melissa C. Leichter, Cheryl Harrington, Laura C. Kasai, Shinji Tomita, Takashi Scott, Jeffrey G. |
| description | The cytochrome P450-dependent monooxygenases (P450s) are an important enzymatic system that metabolizes xenobiotics (e.g., pesticides), as well as endogenous compounds (e.g., hormones). P450-mediated metabolism can result in detoxification of insecticides such as pyrethroids, or can be involved in the bioactivation and detoxification of insecticides such as organophosphates. We isolated (from the JPAL strain) a permethrin resistant strain (ISOP450) of
Culex pipiens quinquefasciatus, having 1300-fold permethrin resistance using standard backcrossing procedures. ISOP450 is highly related to the susceptible lab strain (SLAB) and the high resistance to permethrin is due solely to P450-mediated detoxification. This is the first time in mosquitoes that P450 monooxygenase involvement in pyrethroid resistance has been isolated and studied without the confounding effects of
kdr. Resistance in ISOP450 is incompletely dominant (
D
=
+0.3), autosomally linked, and monofactorally inherited. It is expressed in the larvae, but not in adults. Cross-resistance to pyrethroids lacking a 3-phenoxybenzyl moiety (tetramethrin, fenfluthrin, bioallethrin, and bifenthrin) ranged from 1.5- to 12-fold. ISOP450 had only limited (6.6- and 11-fold) cross-resistance to 3-phenoxybenzyl pyrethroids with an α-cyano group (cypermethrin and deltamethrin, respectively). Examination of cross-resistance patterns to organophosphate insecticides in ISOP450 showed an 8-fold resistance to fenitrothion, while low, but significant, levels of negative cross-resistance were found for malathion (
RR
=
0.84), temephos (
RR
=
0.73), and methyl-parathion (
RR
=
0.55). The importance and uniqueness of this P450 mechanism in insecticide resistance is discussed. |
| doi_str_mv | 10.1016/j.pestbp.2007.06.006 |
| format | Article |
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Culex pipiens quinquefasciatus, having 1300-fold permethrin resistance using standard backcrossing procedures. ISOP450 is highly related to the susceptible lab strain (SLAB) and the high resistance to permethrin is due solely to P450-mediated detoxification. This is the first time in mosquitoes that P450 monooxygenase involvement in pyrethroid resistance has been isolated and studied without the confounding effects of
kdr. Resistance in ISOP450 is incompletely dominant (
D
=
+0.3), autosomally linked, and monofactorally inherited. It is expressed in the larvae, but not in adults. Cross-resistance to pyrethroids lacking a 3-phenoxybenzyl moiety (tetramethrin, fenfluthrin, bioallethrin, and bifenthrin) ranged from 1.5- to 12-fold. ISOP450 had only limited (6.6- and 11-fold) cross-resistance to 3-phenoxybenzyl pyrethroids with an α-cyano group (cypermethrin and deltamethrin, respectively). Examination of cross-resistance patterns to organophosphate insecticides in ISOP450 showed an 8-fold resistance to fenitrothion, while low, but significant, levels of negative cross-resistance were found for malathion (
RR
=
0.84), temephos (
RR
=
0.73), and methyl-parathion (
RR
=
0.55). The importance and uniqueness of this P450 mechanism in insecticide resistance is discussed.</description><identifier>ISSN: 0048-3575</identifier><identifier>EISSN: 1095-9939</identifier><identifier>DOI: 10.1016/j.pestbp.2007.06.006</identifier><identifier>CODEN: PCBPBS</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Control ; cross resistance ; Culex pipiens quinquefasciatus ; Culex quinquefasciatus ; cytochrome P-450 ; Cytochrome P450-mediated metabolism ; Fundamental and applied biological sciences. Psychology ; inheritance (genetics) ; Insecticide resistance mechanism ; kdr ; Life stage specific resistance ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; metabolism ; organophosphorus insecticides ; permethrin ; Phytopathology. Animal pests. Plant and forest protection ; polymerase chain reaction ; Protozoa. Invertebrates ; strains ; Vectors. Intermediate hosts</subject><ispartof>Pesticide biochemistry and physiology, 2007-11, Vol.89 (3), p.175-184</ispartof><rights>2007 Elsevier Inc.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-e114162846b6c293af97194ac04fcebe8afb94cfb56bcb7b071684b6a9e110523</citedby><cites>FETCH-LOGICAL-c457t-e114162846b6c293af97194ac04fcebe8afb94cfb56bcb7b071684b6a9e110523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.pestbp.2007.06.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19132859$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Hardstone, Melissa C.</creatorcontrib><creatorcontrib>Leichter, Cheryl</creatorcontrib><creatorcontrib>Harrington, Laura C.</creatorcontrib><creatorcontrib>Kasai, Shinji</creatorcontrib><creatorcontrib>Tomita, Takashi</creatorcontrib><creatorcontrib>Scott, Jeffrey G.</creatorcontrib><title>Cytochrome P450 monooxygenase-mediated permethrin resistance confers limited and larval specific cross-resistance in the southern house mosquito, Culex pipiens quinquefasciatus</title><title>Pesticide biochemistry and physiology</title><description>The cytochrome P450-dependent monooxygenases (P450s) are an important enzymatic system that metabolizes xenobiotics (e.g., pesticides), as well as endogenous compounds (e.g., hormones). P450-mediated metabolism can result in detoxification of insecticides such as pyrethroids, or can be involved in the bioactivation and detoxification of insecticides such as organophosphates. We isolated (from the JPAL strain) a permethrin resistant strain (ISOP450) of
Culex pipiens quinquefasciatus, having 1300-fold permethrin resistance using standard backcrossing procedures. ISOP450 is highly related to the susceptible lab strain (SLAB) and the high resistance to permethrin is due solely to P450-mediated detoxification. This is the first time in mosquitoes that P450 monooxygenase involvement in pyrethroid resistance has been isolated and studied without the confounding effects of
kdr. Resistance in ISOP450 is incompletely dominant (
D
=
+0.3), autosomally linked, and monofactorally inherited. It is expressed in the larvae, but not in adults. Cross-resistance to pyrethroids lacking a 3-phenoxybenzyl moiety (tetramethrin, fenfluthrin, bioallethrin, and bifenthrin) ranged from 1.5- to 12-fold. ISOP450 had only limited (6.6- and 11-fold) cross-resistance to 3-phenoxybenzyl pyrethroids with an α-cyano group (cypermethrin and deltamethrin, respectively). Examination of cross-resistance patterns to organophosphate insecticides in ISOP450 showed an 8-fold resistance to fenitrothion, while low, but significant, levels of negative cross-resistance were found for malathion (
RR
=
0.84), temephos (
RR
=
0.73), and methyl-parathion (
RR
=
0.55). The importance and uniqueness of this P450 mechanism in insecticide resistance is discussed.</description><subject>Biological and medical sciences</subject><subject>Control</subject><subject>cross resistance</subject><subject>Culex pipiens quinquefasciatus</subject><subject>Culex quinquefasciatus</subject><subject>cytochrome P-450</subject><subject>Cytochrome P450-mediated metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>inheritance (genetics)</subject><subject>Insecticide resistance mechanism</subject><subject>kdr</subject><subject>Life stage specific resistance</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>metabolism</subject><subject>organophosphorus insecticides</subject><subject>permethrin</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>polymerase chain reaction</subject><subject>Protozoa. Invertebrates</subject><subject>strains</subject><subject>Vectors. Intermediate hosts</subject><issn>0048-3575</issn><issn>1095-9939</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kc-K1TAUxosoeB19A8FsdGXrSZukzUaQi84IAwo665CmJ3NzaZNO0g5z38pHNNcO6MrVgcPvO3--ryheU6goUPHhWM2Yln6uaoC2AlEBiCfFjoLkpZSNfFrsAFhXNrzlz4sXKR0BQDKQu-LX_rQEc4hhQvKdcSBT8CE8nG7R64TlhIPTCw5kxjjhcojOk4jJpUV7g8QEbzEmMrrJnSntBzLqeK9HkmY0zjpDTAwplf-I8ojlgCSFNZfoySGsCfPedLe6Jbwn-3XEBzK72aFPJDf93YpWJ5MvWdPL4pnVY8JXj_WiuPny-ef-qrz-dvl1_-m6NIy3S4mUMirqjolemFo22sqWSqYNMGuwx07bXjJjey5607c9tFR0rBdaZiXwurko3m1z5xjy_rSoySWD46g95oNVDZ3gjPIMsg3882hEq-boJh1PioI6x6OOaotHneNRIFSOJ8vePs7Pn-nRxuyNS3-1kjZ1x2Xm3myc1UHp25iZmx810AagayhtIRMfNwKzHfcOo8pWYXZ6cBHNoobg_n_KbzNhtv4</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Hardstone, Melissa C.</creator><creator>Leichter, Cheryl</creator><creator>Harrington, Laura C.</creator><creator>Kasai, Shinji</creator><creator>Tomita, Takashi</creator><creator>Scott, Jeffrey G.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>20071101</creationdate><title>Cytochrome P450 monooxygenase-mediated permethrin resistance confers limited and larval specific cross-resistance in the southern house mosquito, Culex pipiens quinquefasciatus</title><author>Hardstone, Melissa C. ; Leichter, Cheryl ; Harrington, Laura C. ; Kasai, Shinji ; Tomita, Takashi ; Scott, Jeffrey G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-e114162846b6c293af97194ac04fcebe8afb94cfb56bcb7b071684b6a9e110523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biological and medical sciences</topic><topic>Control</topic><topic>cross resistance</topic><topic>Culex pipiens quinquefasciatus</topic><topic>Culex quinquefasciatus</topic><topic>cytochrome P-450</topic><topic>Cytochrome P450-mediated metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>inheritance (genetics)</topic><topic>Insecticide resistance mechanism</topic><topic>kdr</topic><topic>Life stage specific resistance</topic><topic>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</topic><topic>metabolism</topic><topic>organophosphorus insecticides</topic><topic>permethrin</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>polymerase chain reaction</topic><topic>Protozoa. Invertebrates</topic><topic>strains</topic><topic>Vectors. Intermediate hosts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hardstone, Melissa C.</creatorcontrib><creatorcontrib>Leichter, Cheryl</creatorcontrib><creatorcontrib>Harrington, Laura C.</creatorcontrib><creatorcontrib>Kasai, Shinji</creatorcontrib><creatorcontrib>Tomita, Takashi</creatorcontrib><creatorcontrib>Scott, Jeffrey G.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Pesticide biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hardstone, Melissa C.</au><au>Leichter, Cheryl</au><au>Harrington, Laura C.</au><au>Kasai, Shinji</au><au>Tomita, Takashi</au><au>Scott, Jeffrey G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytochrome P450 monooxygenase-mediated permethrin resistance confers limited and larval specific cross-resistance in the southern house mosquito, Culex pipiens quinquefasciatus</atitle><jtitle>Pesticide biochemistry and physiology</jtitle><date>2007-11-01</date><risdate>2007</risdate><volume>89</volume><issue>3</issue><spage>175</spage><epage>184</epage><pages>175-184</pages><issn>0048-3575</issn><eissn>1095-9939</eissn><coden>PCBPBS</coden><abstract>The cytochrome P450-dependent monooxygenases (P450s) are an important enzymatic system that metabolizes xenobiotics (e.g., pesticides), as well as endogenous compounds (e.g., hormones). P450-mediated metabolism can result in detoxification of insecticides such as pyrethroids, or can be involved in the bioactivation and detoxification of insecticides such as organophosphates. We isolated (from the JPAL strain) a permethrin resistant strain (ISOP450) of
Culex pipiens quinquefasciatus, having 1300-fold permethrin resistance using standard backcrossing procedures. ISOP450 is highly related to the susceptible lab strain (SLAB) and the high resistance to permethrin is due solely to P450-mediated detoxification. This is the first time in mosquitoes that P450 monooxygenase involvement in pyrethroid resistance has been isolated and studied without the confounding effects of
kdr. Resistance in ISOP450 is incompletely dominant (
D
=
+0.3), autosomally linked, and monofactorally inherited. It is expressed in the larvae, but not in adults. Cross-resistance to pyrethroids lacking a 3-phenoxybenzyl moiety (tetramethrin, fenfluthrin, bioallethrin, and bifenthrin) ranged from 1.5- to 12-fold. ISOP450 had only limited (6.6- and 11-fold) cross-resistance to 3-phenoxybenzyl pyrethroids with an α-cyano group (cypermethrin and deltamethrin, respectively). Examination of cross-resistance patterns to organophosphate insecticides in ISOP450 showed an 8-fold resistance to fenitrothion, while low, but significant, levels of negative cross-resistance were found for malathion (
RR
=
0.84), temephos (
RR
=
0.73), and methyl-parathion (
RR
=
0.55). The importance and uniqueness of this P450 mechanism in insecticide resistance is discussed.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><doi>10.1016/j.pestbp.2007.06.006</doi><tpages>10</tpages></addata></record> |
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| ispartof | Pesticide biochemistry and physiology, 2007-11, Vol.89 (3), p.175-184 |
| issn | 0048-3575 1095-9939 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Control cross resistance Culex pipiens quinquefasciatus Culex quinquefasciatus cytochrome P-450 Cytochrome P450-mediated metabolism Fundamental and applied biological sciences. Psychology inheritance (genetics) Insecticide resistance mechanism kdr Life stage specific resistance Medically important nuisances and vectors, pests of stored products and materials: population survey and control metabolism organophosphorus insecticides permethrin Phytopathology. Animal pests. Plant and forest protection polymerase chain reaction Protozoa. Invertebrates strains Vectors. Intermediate hosts |
| title | Cytochrome P450 monooxygenase-mediated permethrin resistance confers limited and larval specific cross-resistance in the southern house mosquito, Culex pipiens quinquefasciatus |
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