Nitric oxide (NO) as an intermediate in the cryptogein‐induced hypersensitive response – a critical re‐evaluation
ABSTRACT A hypersensitive response (HR) was induced in tobacco leaves and cell suspensions by the fungal elicitor cryptogein, and NO production was followed by chemiluminescence and occasionally by diaminofluorescein (DAF)‐fluorescence. Results from both methods were at least partly consistent, but...
Gespeichert in:
| Veröffentlicht in: | Plant, cell and environment cell and environment, 2006-01, Vol.29 (1), p.59-69 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 69 |
|---|---|
| container_issue | 1 |
| container_start_page | 59 |
| container_title | Plant, cell and environment |
| container_volume | 29 |
| creator | PLANCHET, ELISABETH SONODA, MASATOSHI ZEIER, JÜRGEN KAISER, WERNER M. |
| description | ABSTRACT
A hypersensitive response (HR) was induced in tobacco leaves and cell suspensions by the fungal elicitor cryptogein, and NO production was followed by chemiluminescence and occasionally by diaminofluorescein (DAF)‐fluorescence. Results from both methods were at least partly consistent, but kinetics was different. NO emission was not induced by cryptogein in leaves, whereas in cell suspensions some weak NO emission was observed, which was nitrate reductase (NR)‐dependent, but not required for cell death. Nitric oxide synthase (NOS) inhibitors did not prevent cell death, but PR‐1 expression was weakened. In conclusion, neither NR nor NOS appear obligatory for the cryptogein‐induced HR. However, a role for NO was still suggested by the fact that the NO scavenger cPTIO prevented the HR. Unexpectedly, cPTI, the reaction product of cPTIO and NO, also impaired the HR but without scavenging NO. Thus, prevention of the HR by cPTIO is not necessarily indicative for a role of NO. Further, even a 100‐fold NO overproduction (over wild type) by a nitrite reductase‐deficient mutant did not interfere with the cryptogein‐induced HR. Accordingly, the role of NO in the HR should be reconsidered. |
| doi_str_mv | 10.1111/j.1365-3040.2005.01400.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_20858921</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20858921</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5050-95cc99beee2e7426254428daad7c02031ef60cf839ef94bfb6c374af6059c4eb3</originalsourceid><addsrcrecordid>eNqNkc9u1DAQxi0EokvhFZCFBIJDwsR24vjAAa3KH6lqOcDZcpwJ9SrrBDtpd299BCTesE-Cw64AccIXez7_vtFoPkJoAXmRzutNXvCqzDgIyBlAmUMhAPLdPbL6_XGfrBY1k1IVJ-RRjBuAJEj1kJwUEupKlnxFbi7cFJylw861SF9eXL6iJlLjqfMThi22zkyYCjpdIbVhP07DV3T-7va78-1ssaVX-xFDRB_d5K6RBozj4CPSu9sf1CRLkq3pk548eG362Uxu8I_Jg870EZ8c71Py5d3Z5_WH7Pzy_cf12_PMllBCpkprlWoQkaEUrGKlEKxujWmlBQa8wK4C29VcYadE0zWV5VKYJJbKCmz4KXlx6DuG4duMcdJbFy32vfE4zFEzqMtasSKBz_4BN8McfJpNM15BrWSlElQfIBuGGAN2egxua8JeF6CXZPRGLwHoJQC9JKN_JaN3yfr02H9u0lr_GI9RJOD5ETAxLawLxlsX_-KEZIKzxL05cDeux_1_D6A_rc-WF_8Japis6g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>236089769</pqid></control><display><type>article</type><title>Nitric oxide (NO) as an intermediate in the cryptogein‐induced hypersensitive response – a critical re‐evaluation</title><source>MEDLINE</source><source>Wiley Online Library Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library All Journals</source><source>Alma/SFX Local Collection</source><creator>PLANCHET, ELISABETH ; SONODA, MASATOSHI ; ZEIER, JÜRGEN ; KAISER, WERNER M.</creator><creatorcontrib>PLANCHET, ELISABETH ; SONODA, MASATOSHI ; ZEIER, JÜRGEN ; KAISER, WERNER M.</creatorcontrib><description>ABSTRACT
A hypersensitive response (HR) was induced in tobacco leaves and cell suspensions by the fungal elicitor cryptogein, and NO production was followed by chemiluminescence and occasionally by diaminofluorescein (DAF)‐fluorescence. Results from both methods were at least partly consistent, but kinetics was different. NO emission was not induced by cryptogein in leaves, whereas in cell suspensions some weak NO emission was observed, which was nitrate reductase (NR)‐dependent, but not required for cell death. Nitric oxide synthase (NOS) inhibitors did not prevent cell death, but PR‐1 expression was weakened. In conclusion, neither NR nor NOS appear obligatory for the cryptogein‐induced HR. However, a role for NO was still suggested by the fact that the NO scavenger cPTIO prevented the HR. Unexpectedly, cPTI, the reaction product of cPTIO and NO, also impaired the HR but without scavenging NO. Thus, prevention of the HR by cPTIO is not necessarily indicative for a role of NO. Further, even a 100‐fold NO overproduction (over wild type) by a nitrite reductase‐deficient mutant did not interfere with the cryptogein‐induced HR. Accordingly, the role of NO in the HR should be reconsidered.</description><identifier>ISSN: 0140-7791</identifier><identifier>EISSN: 1365-3040</identifier><identifier>DOI: 10.1111/j.1365-3040.2005.01400.x</identifier><identifier>PMID: 17086753</identifier><identifier>CODEN: PLCEDV</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Algal Proteins - pharmacology ; Benzoates - pharmacology ; Biological and medical sciences ; Cell Death - drug effects ; chemiluminescence detection ; cryptogein ; Cyclic N-Oxides - pharmacology ; DAF‐2DA ; Fluorescence ; Free Radical Scavengers - metabolism ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins ; Gene Expression Regulation, Plant - drug effects ; Generalities. Disease free stocks ; hypersensitive response ; Imidazoles - pharmacology ; Nicotiana - drug effects ; Nicotiana tabacum ; nitrate reductase ; Nitrate Reductase - metabolism ; nitric oxide ; Nitric Oxide - metabolism ; nitric oxide synthase ; Nitric Oxide Synthase - antagonists & inhibitors ; Phytopathology. Animal pests. Plant and forest protection ; Plant Leaves - drug effects ; Plant Proteins - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Time Factors</subject><ispartof>Plant, cell and environment, 2006-01, Vol.29 (1), p.59-69</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Jan 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5050-95cc99beee2e7426254428daad7c02031ef60cf839ef94bfb6c374af6059c4eb3</citedby><cites>FETCH-LOGICAL-c5050-95cc99beee2e7426254428daad7c02031ef60cf839ef94bfb6c374af6059c4eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-3040.2005.01400.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-3040.2005.01400.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,4014,27914,27915,27916,45565,45566,46400,46824</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17472432$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17086753$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PLANCHET, ELISABETH</creatorcontrib><creatorcontrib>SONODA, MASATOSHI</creatorcontrib><creatorcontrib>ZEIER, JÜRGEN</creatorcontrib><creatorcontrib>KAISER, WERNER M.</creatorcontrib><title>Nitric oxide (NO) as an intermediate in the cryptogein‐induced hypersensitive response – a critical re‐evaluation</title><title>Plant, cell and environment</title><addtitle>Plant Cell Environ</addtitle><description>ABSTRACT
A hypersensitive response (HR) was induced in tobacco leaves and cell suspensions by the fungal elicitor cryptogein, and NO production was followed by chemiluminescence and occasionally by diaminofluorescein (DAF)‐fluorescence. Results from both methods were at least partly consistent, but kinetics was different. NO emission was not induced by cryptogein in leaves, whereas in cell suspensions some weak NO emission was observed, which was nitrate reductase (NR)‐dependent, but not required for cell death. Nitric oxide synthase (NOS) inhibitors did not prevent cell death, but PR‐1 expression was weakened. In conclusion, neither NR nor NOS appear obligatory for the cryptogein‐induced HR. However, a role for NO was still suggested by the fact that the NO scavenger cPTIO prevented the HR. Unexpectedly, cPTI, the reaction product of cPTIO and NO, also impaired the HR but without scavenging NO. Thus, prevention of the HR by cPTIO is not necessarily indicative for a role of NO. Further, even a 100‐fold NO overproduction (over wild type) by a nitrite reductase‐deficient mutant did not interfere with the cryptogein‐induced HR. Accordingly, the role of NO in the HR should be reconsidered.</description><subject>Algal Proteins - pharmacology</subject><subject>Benzoates - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Death - drug effects</subject><subject>chemiluminescence detection</subject><subject>cryptogein</subject><subject>Cyclic N-Oxides - pharmacology</subject><subject>DAF‐2DA</subject><subject>Fluorescence</subject><subject>Free Radical Scavengers - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Generalities. Disease free stocks</subject><subject>hypersensitive response</subject><subject>Imidazoles - pharmacology</subject><subject>Nicotiana - drug effects</subject><subject>Nicotiana tabacum</subject><subject>nitrate reductase</subject><subject>Nitrate Reductase - metabolism</subject><subject>nitric oxide</subject><subject>Nitric Oxide - metabolism</subject><subject>nitric oxide synthase</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant Leaves - drug effects</subject><subject>Plant Proteins - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Time Factors</subject><issn>0140-7791</issn><issn>1365-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQxi0EokvhFZCFBIJDwsR24vjAAa3KH6lqOcDZcpwJ9SrrBDtpd299BCTesE-Cw64AccIXez7_vtFoPkJoAXmRzutNXvCqzDgIyBlAmUMhAPLdPbL6_XGfrBY1k1IVJ-RRjBuAJEj1kJwUEupKlnxFbi7cFJylw861SF9eXL6iJlLjqfMThi22zkyYCjpdIbVhP07DV3T-7va78-1ssaVX-xFDRB_d5K6RBozj4CPSu9sf1CRLkq3pk548eG362Uxu8I_Jg870EZ8c71Py5d3Z5_WH7Pzy_cf12_PMllBCpkprlWoQkaEUrGKlEKxujWmlBQa8wK4C29VcYadE0zWV5VKYJJbKCmz4KXlx6DuG4duMcdJbFy32vfE4zFEzqMtasSKBz_4BN8McfJpNM15BrWSlElQfIBuGGAN2egxua8JeF6CXZPRGLwHoJQC9JKN_JaN3yfr02H9u0lr_GI9RJOD5ETAxLawLxlsX_-KEZIKzxL05cDeux_1_D6A_rc-WF_8Japis6g</recordid><startdate>200601</startdate><enddate>200601</enddate><creator>PLANCHET, ELISABETH</creator><creator>SONODA, MASATOSHI</creator><creator>ZEIER, JÜRGEN</creator><creator>KAISER, WERNER M.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7T7</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>200601</creationdate><title>Nitric oxide (NO) as an intermediate in the cryptogein‐induced hypersensitive response – a critical re‐evaluation</title><author>PLANCHET, ELISABETH ; SONODA, MASATOSHI ; ZEIER, JÜRGEN ; KAISER, WERNER M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5050-95cc99beee2e7426254428daad7c02031ef60cf839ef94bfb6c374af6059c4eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Algal Proteins - pharmacology</topic><topic>Benzoates - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Death - drug effects</topic><topic>chemiluminescence detection</topic><topic>cryptogein</topic><topic>Cyclic N-Oxides - pharmacology</topic><topic>DAF‐2DA</topic><topic>Fluorescence</topic><topic>Free Radical Scavengers - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Generalities. Disease free stocks</topic><topic>hypersensitive response</topic><topic>Imidazoles - pharmacology</topic><topic>Nicotiana - drug effects</topic><topic>Nicotiana tabacum</topic><topic>nitrate reductase</topic><topic>Nitrate Reductase - metabolism</topic><topic>nitric oxide</topic><topic>Nitric Oxide - metabolism</topic><topic>nitric oxide synthase</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant Leaves - drug effects</topic><topic>Plant Proteins - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PLANCHET, ELISABETH</creatorcontrib><creatorcontrib>SONODA, MASATOSHI</creatorcontrib><creatorcontrib>ZEIER, JÜRGEN</creatorcontrib><creatorcontrib>KAISER, WERNER M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant, cell and environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PLANCHET, ELISABETH</au><au>SONODA, MASATOSHI</au><au>ZEIER, JÜRGEN</au><au>KAISER, WERNER M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric oxide (NO) as an intermediate in the cryptogein‐induced hypersensitive response – a critical re‐evaluation</atitle><jtitle>Plant, cell and environment</jtitle><addtitle>Plant Cell Environ</addtitle><date>2006-01</date><risdate>2006</risdate><volume>29</volume><issue>1</issue><spage>59</spage><epage>69</epage><pages>59-69</pages><issn>0140-7791</issn><eissn>1365-3040</eissn><coden>PLCEDV</coden><abstract>ABSTRACT
A hypersensitive response (HR) was induced in tobacco leaves and cell suspensions by the fungal elicitor cryptogein, and NO production was followed by chemiluminescence and occasionally by diaminofluorescein (DAF)‐fluorescence. Results from both methods were at least partly consistent, but kinetics was different. NO emission was not induced by cryptogein in leaves, whereas in cell suspensions some weak NO emission was observed, which was nitrate reductase (NR)‐dependent, but not required for cell death. Nitric oxide synthase (NOS) inhibitors did not prevent cell death, but PR‐1 expression was weakened. In conclusion, neither NR nor NOS appear obligatory for the cryptogein‐induced HR. However, a role for NO was still suggested by the fact that the NO scavenger cPTIO prevented the HR. Unexpectedly, cPTI, the reaction product of cPTIO and NO, also impaired the HR but without scavenging NO. Thus, prevention of the HR by cPTIO is not necessarily indicative for a role of NO. Further, even a 100‐fold NO overproduction (over wild type) by a nitrite reductase‐deficient mutant did not interfere with the cryptogein‐induced HR. Accordingly, the role of NO in the HR should be reconsidered.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>17086753</pmid><doi>10.1111/j.1365-3040.2005.01400.x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0140-7791 |
| ispartof | Plant, cell and environment, 2006-01, Vol.29 (1), p.59-69 |
| issn | 0140-7791 1365-3040 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_20858921 |
| source | MEDLINE; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Algal Proteins - pharmacology Benzoates - pharmacology Biological and medical sciences Cell Death - drug effects chemiluminescence detection cryptogein Cyclic N-Oxides - pharmacology DAF‐2DA Fluorescence Free Radical Scavengers - metabolism Fundamental and applied biological sciences. Psychology Fungal Proteins Gene Expression Regulation, Plant - drug effects Generalities. Disease free stocks hypersensitive response Imidazoles - pharmacology Nicotiana - drug effects Nicotiana tabacum nitrate reductase Nitrate Reductase - metabolism nitric oxide Nitric Oxide - metabolism nitric oxide synthase Nitric Oxide Synthase - antagonists & inhibitors Phytopathology. Animal pests. Plant and forest protection Plant Leaves - drug effects Plant Proteins - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Time Factors |
| title | Nitric oxide (NO) as an intermediate in the cryptogein‐induced hypersensitive response – a critical re‐evaluation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T00%3A59%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nitric%20oxide%20(NO)%20as%20an%20intermediate%20in%20the%20cryptogein%E2%80%90induced%20hypersensitive%20response%20%E2%80%93%20a%20critical%20re%E2%80%90evaluation&rft.jtitle=Plant,%20cell%20and%20environment&rft.au=PLANCHET,%20ELISABETH&rft.date=2006-01&rft.volume=29&rft.issue=1&rft.spage=59&rft.epage=69&rft.pages=59-69&rft.issn=0140-7791&rft.eissn=1365-3040&rft.coden=PLCEDV&rft_id=info:doi/10.1111/j.1365-3040.2005.01400.x&rft_dat=%3Cproquest_cross%3E20858921%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=236089769&rft_id=info:pmid/17086753&rfr_iscdi=true |