Clustering of nosocomial Candida kefyr infections among hematological patients in a university hospital: Molecular typing of the strains by PFGE and RAPD
This study spreading over a span of eight months described Candida kefyr infections in hematolology patients from a Turkish University hospital. A total of 18 strains were isolated from various body locations of 18 patients. DNA fingerprinting of the isolates was performed by using randomly amplifie...
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| Veröffentlicht in: | Journal de mycologie médicale 2007-12, Vol.17 (4), p.250-255 |
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| creator | Kalkanci, A. Saracli, M.A. Guzel, O. Yildiran, S.T. Senol, E. Kustimur, S. |
| description | This study spreading over a span of eight months described
Candida kefyr infections in hematolology patients from a Turkish University hospital. A total of 18 strains were isolated from various body locations of 18 patients. DNA fingerprinting of the isolates was performed by using randomly amplified polymorphic DNA (RAPD) method and karyotyping by pulsed-field gel electrophoresis (PFGE). RAPD analysis of all the
C.
kefyr strains revealed that strains collected from 18 different patients exhibited seven different genotypes, while those were six in PFGE. Taken together, this is probably the first molecular survey of
C.
kefyr infections in the hematology unit documenting nosocomial transmission of
C.
kefyr isolates.
Cette étude répartie sur huit mois a décrit des infections à
Candida kefyr chez des patients du service hématolologique d’un hôpital universitaire turc. Un total de 18 souches de
C.
kefyr a été isolé sur divers parties du corps de 18 patients. L’analyse moléculaire de l’ADN des isolats a été effectuée par RAPD et le caryotypage par PFGE. L’analyse RAPD des souches de
C.
kefyr prélevées sur 18 patients différents, a indiqué sept différents génotypes, alors que PFGE n’a révélé que six génotypes. C’est probablement la première recherche moléculaire d’infections à
C.
kefyr dans l’unité hématologique sur la transmission nosocomiale d’isolats de
C.
kefyr qui inclut les deux méthodes RAPD et PFGE. |
| doi_str_mv | 10.1016/j.mycmed.2007.09.003 |
| format | Article |
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Candida kefyr infections in hematolology patients from a Turkish University hospital. A total of 18 strains were isolated from various body locations of 18 patients. DNA fingerprinting of the isolates was performed by using randomly amplified polymorphic DNA (RAPD) method and karyotyping by pulsed-field gel electrophoresis (PFGE). RAPD analysis of all the
C.
kefyr strains revealed that strains collected from 18 different patients exhibited seven different genotypes, while those were six in PFGE. Taken together, this is probably the first molecular survey of
C.
kefyr infections in the hematology unit documenting nosocomial transmission of
C.
kefyr isolates.
Cette étude répartie sur huit mois a décrit des infections à
Candida kefyr chez des patients du service hématolologique d’un hôpital universitaire turc. Un total de 18 souches de
C.
kefyr a été isolé sur divers parties du corps de 18 patients. L’analyse moléculaire de l’ADN des isolats a été effectuée par RAPD et le caryotypage par PFGE. L’analyse RAPD des souches de
C.
kefyr prélevées sur 18 patients différents, a indiqué sept différents génotypes, alors que PFGE n’a révélé que six génotypes. C’est probablement la première recherche moléculaire d’infections à
C.
kefyr dans l’unité hématologique sur la transmission nosocomiale d’isolats de
C.
kefyr qui inclut les deux méthodes RAPD et PFGE.</description><identifier>ISSN: 1156-5233</identifier><identifier>EISSN: 1773-0449</identifier><identifier>DOI: 10.1016/j.mycmed.2007.09.003</identifier><language>eng</language><publisher>Paris: Elsevier SAS</publisher><subject>AP-PCR ; Biological and medical sciences ; Candida kefyr ; Fundamental and applied biological sciences. Psychology ; Hematology ; Hématologie ; Infection ; Infection nososcomiale ; Infectious diseases ; Medical sciences ; Microbiology ; Miscellaneous ; Mycology ; Mycoses ; Nososcomial ; Pathogenicity, host-agent relations, miscellaneous strains, epidemiology ; PFGE ; RAPD</subject><ispartof>Journal de mycologie médicale, 2007-12, Vol.17 (4), p.250-255</ispartof><rights>2007 Elsevier Masson SAS</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-9322314e9b6d6aa7bd10ec362cc6a31a795ef99e173eb9d440ddedc4b2f4ea313</citedby><cites>FETCH-LOGICAL-c367t-9322314e9b6d6aa7bd10ec362cc6a31a795ef99e173eb9d440ddedc4b2f4ea313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mycmed.2007.09.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19904701$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kalkanci, A.</creatorcontrib><creatorcontrib>Saracli, M.A.</creatorcontrib><creatorcontrib>Guzel, O.</creatorcontrib><creatorcontrib>Yildiran, S.T.</creatorcontrib><creatorcontrib>Senol, E.</creatorcontrib><creatorcontrib>Kustimur, S.</creatorcontrib><title>Clustering of nosocomial Candida kefyr infections among hematological patients in a university hospital: Molecular typing of the strains by PFGE and RAPD</title><title>Journal de mycologie médicale</title><description>This study spreading over a span of eight months described
Candida kefyr infections in hematolology patients from a Turkish University hospital. A total of 18 strains were isolated from various body locations of 18 patients. DNA fingerprinting of the isolates was performed by using randomly amplified polymorphic DNA (RAPD) method and karyotyping by pulsed-field gel electrophoresis (PFGE). RAPD analysis of all the
C.
kefyr strains revealed that strains collected from 18 different patients exhibited seven different genotypes, while those were six in PFGE. Taken together, this is probably the first molecular survey of
C.
kefyr infections in the hematology unit documenting nosocomial transmission of
C.
kefyr isolates.
Cette étude répartie sur huit mois a décrit des infections à
Candida kefyr chez des patients du service hématolologique d’un hôpital universitaire turc. Un total de 18 souches de
C.
kefyr a été isolé sur divers parties du corps de 18 patients. L’analyse moléculaire de l’ADN des isolats a été effectuée par RAPD et le caryotypage par PFGE. L’analyse RAPD des souches de
C.
kefyr prélevées sur 18 patients différents, a indiqué sept différents génotypes, alors que PFGE n’a révélé que six génotypes. C’est probablement la première recherche moléculaire d’infections à
C.
kefyr dans l’unité hématologique sur la transmission nosocomiale d’isolats de
C.
kefyr qui inclut les deux méthodes RAPD et PFGE.</description><subject>AP-PCR</subject><subject>Biological and medical sciences</subject><subject>Candida kefyr</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hematology</subject><subject>Hématologie</subject><subject>Infection</subject><subject>Infection nososcomiale</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Mycoses</subject><subject>Nososcomial</subject><subject>Pathogenicity, host-agent relations, miscellaneous strains, epidemiology</subject><subject>PFGE</subject><subject>RAPD</subject><issn>1156-5233</issn><issn>1773-0449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kUGP1SAUhRujiePoP3DBRnetUGgrLkwmz5nRZIwTo2tyC7fzeFKoQCfpT_Hfyst7iTtXkPDdcy7nVNVrRhtGWf_u0MybntE0LaVDQ2VDKX9SXbBh4DUVQj4td9b1dddy_rx6kdKB0q7vOnFR_dm5NWWM1j-QMBEfUtBhtuDIDryxBsgvnLZIrJ9QZxt8IjCHAu9xhhxceLC6wAtkiz6nwhEgq7ePGJPNG9mHtNgM7gP5Ghzq1UEkeVvOdnmPJOUItsiOG7m_ub0mxZZ8v7r_9LJ6NoFL-Op8XlY_b65_7D7Xd99uv-yu7mrN-yHXkrctZwLl2JseYBgNo1ieWq174AwG2eEkJbKB4yiNENQYNFqM7SSwAPyyenvSXWL4vWLKarZJo3PgMaxJtfR9xzvRF1CcQB1DShEntUQ7Q9wUo-rYgzqoUw_q2IOiUpUeytibsz6kEtUUwWub_s1KScVAj3t8PHFYPvtoMaqkS6YajY0lemWC_b_RX26eo4Q</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Kalkanci, A.</creator><creator>Saracli, M.A.</creator><creator>Guzel, O.</creator><creator>Yildiran, S.T.</creator><creator>Senol, E.</creator><creator>Kustimur, S.</creator><general>Elsevier SAS</general><general>Masson</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>20071201</creationdate><title>Clustering of nosocomial Candida kefyr infections among hematological patients in a university hospital: Molecular typing of the strains by PFGE and RAPD</title><author>Kalkanci, A. ; Saracli, M.A. ; Guzel, O. ; Yildiran, S.T. ; Senol, E. ; Kustimur, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-9322314e9b6d6aa7bd10ec362cc6a31a795ef99e173eb9d440ddedc4b2f4ea313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>AP-PCR</topic><topic>Biological and medical sciences</topic><topic>Candida kefyr</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hematology</topic><topic>Hématologie</topic><topic>Infection</topic><topic>Infection nososcomiale</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Mycoses</topic><topic>Nososcomial</topic><topic>Pathogenicity, host-agent relations, miscellaneous strains, epidemiology</topic><topic>PFGE</topic><topic>RAPD</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kalkanci, A.</creatorcontrib><creatorcontrib>Saracli, M.A.</creatorcontrib><creatorcontrib>Guzel, O.</creatorcontrib><creatorcontrib>Yildiran, S.T.</creatorcontrib><creatorcontrib>Senol, E.</creatorcontrib><creatorcontrib>Kustimur, S.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal de mycologie médicale</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kalkanci, A.</au><au>Saracli, M.A.</au><au>Guzel, O.</au><au>Yildiran, S.T.</au><au>Senol, E.</au><au>Kustimur, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clustering of nosocomial Candida kefyr infections among hematological patients in a university hospital: Molecular typing of the strains by PFGE and RAPD</atitle><jtitle>Journal de mycologie médicale</jtitle><date>2007-12-01</date><risdate>2007</risdate><volume>17</volume><issue>4</issue><spage>250</spage><epage>255</epage><pages>250-255</pages><issn>1156-5233</issn><eissn>1773-0449</eissn><abstract>This study spreading over a span of eight months described
Candida kefyr infections in hematolology patients from a Turkish University hospital. A total of 18 strains were isolated from various body locations of 18 patients. DNA fingerprinting of the isolates was performed by using randomly amplified polymorphic DNA (RAPD) method and karyotyping by pulsed-field gel electrophoresis (PFGE). RAPD analysis of all the
C.
kefyr strains revealed that strains collected from 18 different patients exhibited seven different genotypes, while those were six in PFGE. Taken together, this is probably the first molecular survey of
C.
kefyr infections in the hematology unit documenting nosocomial transmission of
C.
kefyr isolates.
Cette étude répartie sur huit mois a décrit des infections à
Candida kefyr chez des patients du service hématolologique d’un hôpital universitaire turc. Un total de 18 souches de
C.
kefyr a été isolé sur divers parties du corps de 18 patients. L’analyse moléculaire de l’ADN des isolats a été effectuée par RAPD et le caryotypage par PFGE. L’analyse RAPD des souches de
C.
kefyr prélevées sur 18 patients différents, a indiqué sept différents génotypes, alors que PFGE n’a révélé que six génotypes. C’est probablement la première recherche moléculaire d’infections à
C.
kefyr dans l’unité hématologique sur la transmission nosocomiale d’isolats de
C.
kefyr qui inclut les deux méthodes RAPD et PFGE.</abstract><cop>Paris</cop><pub>Elsevier SAS</pub><doi>10.1016/j.mycmed.2007.09.003</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal de mycologie médicale, 2007-12, Vol.17 (4), p.250-255 |
| issn | 1156-5233 1773-0449 |
| language | eng |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | AP-PCR Biological and medical sciences Candida kefyr Fundamental and applied biological sciences. Psychology Hematology Hématologie Infection Infection nososcomiale Infectious diseases Medical sciences Microbiology Miscellaneous Mycology Mycoses Nososcomial Pathogenicity, host-agent relations, miscellaneous strains, epidemiology PFGE RAPD |
| title | Clustering of nosocomial Candida kefyr infections among hematological patients in a university hospital: Molecular typing of the strains by PFGE and RAPD |
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