Microspore-derived cell suspension cultures of oilseed rape as a system for studying gene expression
Abiotic stress, such as extreme temperature, drought, or excessive salinity, is one of the leading causes of crop loss worldwide. Microspore-derived (MD) cell suspension cultures of Brassica napus L. cv. Jet Neuf have been shown to be a useful system for studying the biochemistry of developing oilse...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2008-02, Vol.92 (2), p.131-139 |
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| container_title | Plant cell, tissue and organ culture |
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| creator | Shi, Yongzhong Xu, Gangbiao Warrington, Timothy B. Murdoch, Gordon K. Kazala, E. Chris Snyder, Crystal L. Weselake, Randall J. |
| description | Abiotic stress, such as extreme temperature, drought, or excessive salinity, is one of the leading causes of crop loss worldwide. Microspore-derived (MD) cell suspension cultures of
Brassica napus
L. cv. Jet Neuf have been shown to be a useful system for studying the biochemistry of developing oilseeds. In the present study, we describe the application of MD cell suspension cultures of
B. napus
as a system for studying gene expression in response to abiotic stress, and demonstrate emybryogenic competence in cultures that have been continuously subcultured for more than 20 years. MD cell suspension cultures of
B. napus
L. cv Jet Neuf were exposed to low temperature or osmotic stress and the expression profile of known stress responsive genes was evaluated. The gene expression profile of
BN115
, a known cold-responsive gene in
B. napus
, was similar to that described for intact cold-acclimated plants. Likewise, two late embryogenesis abundant (
Lea
) genes were shown to be up-regulated in response to low temperature or osmotic stress. The results demonstrate that
B. napus
MD cell suspension cultures are a useful system for the investigation of changes in gene expression in plants brought about by abiotic stress. |
| doi_str_mv | 10.1007/s11240-007-9313-5 |
| format | Article |
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Brassica napus
L. cv. Jet Neuf have been shown to be a useful system for studying the biochemistry of developing oilseeds. In the present study, we describe the application of MD cell suspension cultures of
B. napus
as a system for studying gene expression in response to abiotic stress, and demonstrate emybryogenic competence in cultures that have been continuously subcultured for more than 20 years. MD cell suspension cultures of
B. napus
L. cv Jet Neuf were exposed to low temperature or osmotic stress and the expression profile of known stress responsive genes was evaluated. The gene expression profile of
BN115
, a known cold-responsive gene in
B. napus
, was similar to that described for intact cold-acclimated plants. Likewise, two late embryogenesis abundant (
Lea
) genes were shown to be up-regulated in response to low temperature or osmotic stress. The results demonstrate that
B. napus
MD cell suspension cultures are a useful system for the investigation of changes in gene expression in plants brought about by abiotic stress.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-007-9313-5</identifier><identifier>CODEN: PTCEDJ</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Brassica napus ; Fundamental and applied biological sciences. Psychology ; Life Sciences ; Original Paper ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences</subject><ispartof>Plant cell, tissue and organ culture, 2008-02, Vol.92 (2), p.131-139</ispartof><rights>Springer Science+Business Media B.V. 2007</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-c81a0ca813ffb9cd0a8764b8bf4b5582a1fe9b8ff5056494049756ec1c13480e3</citedby><cites>FETCH-LOGICAL-c349t-c81a0ca813ffb9cd0a8764b8bf4b5582a1fe9b8ff5056494049756ec1c13480e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-007-9313-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-007-9313-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19964583$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Yongzhong</creatorcontrib><creatorcontrib>Xu, Gangbiao</creatorcontrib><creatorcontrib>Warrington, Timothy B.</creatorcontrib><creatorcontrib>Murdoch, Gordon K.</creatorcontrib><creatorcontrib>Kazala, E. Chris</creatorcontrib><creatorcontrib>Snyder, Crystal L.</creatorcontrib><creatorcontrib>Weselake, Randall J.</creatorcontrib><title>Microspore-derived cell suspension cultures of oilseed rape as a system for studying gene expression</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>Abiotic stress, such as extreme temperature, drought, or excessive salinity, is one of the leading causes of crop loss worldwide. Microspore-derived (MD) cell suspension cultures of
Brassica napus
L. cv. Jet Neuf have been shown to be a useful system for studying the biochemistry of developing oilseeds. In the present study, we describe the application of MD cell suspension cultures of
B. napus
as a system for studying gene expression in response to abiotic stress, and demonstrate emybryogenic competence in cultures that have been continuously subcultured for more than 20 years. MD cell suspension cultures of
B. napus
L. cv Jet Neuf were exposed to low temperature or osmotic stress and the expression profile of known stress responsive genes was evaluated. The gene expression profile of
BN115
, a known cold-responsive gene in
B. napus
, was similar to that described for intact cold-acclimated plants. Likewise, two late embryogenesis abundant (
Lea
) genes were shown to be up-regulated in response to low temperature or osmotic stress. The results demonstrate that
B. napus
MD cell suspension cultures are a useful system for the investigation of changes in gene expression in plants brought about by abiotic stress.</description><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Brassica napus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Life Sciences</subject><subject>Original Paper</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LxDAUxIMouK5-AG-56K2atEmaHmXxH6x40XNI05elS7epea24396UXfDm6Q283wzMEHLN2R1nrLxHznPBsiSzquBFJk_IgssyCSbEKVkwrspMaVmekwvELWNMFYIvSPPWuhhwCBGyBmL7DQ110HUUJxygxzb01E3dOEVAGjwNbYeQmGgHoBappbjHEXbUh0hxnJp922_oBnqg8DMk05xwSc68Tb6r412Sz6fHj9VLtn5_fl09rDNXiGrMnOaWOat54X1duYZZXSpR69qLWkqdW-6hqrX3kkklKsFEVUoFjjteCM2gWJLbQ-4Qw9cEOJpdi3Mb20OY0ORMCyWVTiA_gHN3jODNENudjXvDmZn3NIc9zSznPY1MnptjuEVnOx9t71r8M1aVElIXicsPHKZXv4FotmGKfer9T_gvySyG9Q</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Shi, Yongzhong</creator><creator>Xu, Gangbiao</creator><creator>Warrington, Timothy B.</creator><creator>Murdoch, Gordon K.</creator><creator>Kazala, E. Chris</creator><creator>Snyder, Crystal L.</creator><creator>Weselake, Randall J.</creator><general>Springer Netherlands</general><general>Springer</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20080201</creationdate><title>Microspore-derived cell suspension cultures of oilseed rape as a system for studying gene expression</title><author>Shi, Yongzhong ; Xu, Gangbiao ; Warrington, Timothy B. ; Murdoch, Gordon K. ; Kazala, E. Chris ; Snyder, Crystal L. ; Weselake, Randall J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-c81a0ca813ffb9cd0a8764b8bf4b5582a1fe9b8ff5056494049756ec1c13480e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Brassica napus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Life Sciences</topic><topic>Original Paper</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Yongzhong</creatorcontrib><creatorcontrib>Xu, Gangbiao</creatorcontrib><creatorcontrib>Warrington, Timothy B.</creatorcontrib><creatorcontrib>Murdoch, Gordon K.</creatorcontrib><creatorcontrib>Kazala, E. Chris</creatorcontrib><creatorcontrib>Snyder, Crystal L.</creatorcontrib><creatorcontrib>Weselake, Randall J.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Yongzhong</au><au>Xu, Gangbiao</au><au>Warrington, Timothy B.</au><au>Murdoch, Gordon K.</au><au>Kazala, E. Chris</au><au>Snyder, Crystal L.</au><au>Weselake, Randall J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microspore-derived cell suspension cultures of oilseed rape as a system for studying gene expression</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2008-02-01</date><risdate>2008</risdate><volume>92</volume><issue>2</issue><spage>131</spage><epage>139</epage><pages>131-139</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>Abiotic stress, such as extreme temperature, drought, or excessive salinity, is one of the leading causes of crop loss worldwide. Microspore-derived (MD) cell suspension cultures of
Brassica napus
L. cv. Jet Neuf have been shown to be a useful system for studying the biochemistry of developing oilseeds. In the present study, we describe the application of MD cell suspension cultures of
B. napus
as a system for studying gene expression in response to abiotic stress, and demonstrate emybryogenic competence in cultures that have been continuously subcultured for more than 20 years. MD cell suspension cultures of
B. napus
L. cv Jet Neuf were exposed to low temperature or osmotic stress and the expression profile of known stress responsive genes was evaluated. The gene expression profile of
BN115
, a known cold-responsive gene in
B. napus
, was similar to that described for intact cold-acclimated plants. Likewise, two late embryogenesis abundant (
Lea
) genes were shown to be up-regulated in response to low temperature or osmotic stress. The results demonstrate that
B. napus
MD cell suspension cultures are a useful system for the investigation of changes in gene expression in plants brought about by abiotic stress.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-007-9313-5</doi><tpages>9</tpages></addata></record> |
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| ispartof | Plant cell, tissue and organ culture, 2008-02, Vol.92 (2), p.131-139 |
| issn | 0167-6857 1573-5044 |
| language | eng |
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| subjects | Biological and medical sciences Biomedical and Life Sciences Biotechnology Brassica napus Fundamental and applied biological sciences. Psychology Life Sciences Original Paper Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences |
| title | Microspore-derived cell suspension cultures of oilseed rape as a system for studying gene expression |
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