Application of pulsed-field gel electrophoresis to characterise and trace the prevalence of Enterobacter sakazakii in an infant formula processing facility
Enterobacter sakazakii ( E. sakazakii) contamination of powdered infant formula (PIF) and its processing environment was monitored between April 2005 and March 2006. The purpose of the monitoring programme was to locate points of contamination, investigate clonal persistence, and identify possible d...
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| Veröffentlicht in: | International journal of food microbiology 2007-05, Vol.116 (1), p.73-81 |
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| creator | Mullane, N.R. Whyte, P. Wall, P.G. Quinn, T. Fanning, S. |
| description | Enterobacter sakazakii (
E. sakazakii) contamination of powdered infant formula (PIF) and its processing environment was monitored between April 2005 and March 2006. The purpose of the monitoring programme was to locate points of contamination, investigate clonal persistence, and identify possible dissemination routes along the processing chain. A total of 80
E. sakazakii isolates were recovered from the manufacturing facility. The overall frequency of isolation of
E. sakazakii in intermediate and final product was 2.5%, while specific locations in the processing environment were contaminated at frequencies up to 31%. All
E. sakazakii isolates were characterised by pulsed-field gel electrophoresis (PFGE).
XbaI macrorestriction digests yielded 19 unique pulse-types that could be grouped into 6 clusters of between 5 and 32 isolates. The formation of large clusters was consistent with the presence of a number of clones in the manufacturing environment. While the majority of isolates were of environmental origin (72.5%), no cluster was confined to one specific location and indistinguishable PFGE profiles were generated from isolates cultured from the manufacturing environment, sampling points along the processing chain and from intermediate and final product. These findings suggest that the manufacturing environment serves as a key route for sporadic contamination of PIF. These data will support the development of efficient intervention measures contributing to the reduction of
E. sakazakii in the PIF processing chain. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2006.12.036 |
| format | Article |
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E. sakazakii) contamination of powdered infant formula (PIF) and its processing environment was monitored between April 2005 and March 2006. The purpose of the monitoring programme was to locate points of contamination, investigate clonal persistence, and identify possible dissemination routes along the processing chain. A total of 80
E. sakazakii isolates were recovered from the manufacturing facility. The overall frequency of isolation of
E. sakazakii in intermediate and final product was 2.5%, while specific locations in the processing environment were contaminated at frequencies up to 31%. All
E. sakazakii isolates were characterised by pulsed-field gel electrophoresis (PFGE).
XbaI macrorestriction digests yielded 19 unique pulse-types that could be grouped into 6 clusters of between 5 and 32 isolates. The formation of large clusters was consistent with the presence of a number of clones in the manufacturing environment. While the majority of isolates were of environmental origin (72.5%), no cluster was confined to one specific location and indistinguishable PFGE profiles were generated from isolates cultured from the manufacturing environment, sampling points along the processing chain and from intermediate and final product. These findings suggest that the manufacturing environment serves as a key route for sporadic contamination of PIF. These data will support the development of efficient intervention measures contributing to the reduction of
E. sakazakii in the PIF processing chain.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2006.12.036</identifier><identifier>PMID: 17307267</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>bacterial contamination ; Biological and medical sciences ; Cluster Analysis ; Colony Count, Microbial ; Consumer Product Safety ; Cronobacter sakazakii ; Cronobacter sakazakii - classification ; Cronobacter sakazakii - isolation & purification ; Electrophoresis, Gel, Pulsed-Field ; Enterobacter sakazakii ; Environmental Microbiology ; Equipment Contamination - prevention & control ; food contamination ; Food Contamination - analysis ; Food industries ; Food Microbiology ; food pathogens ; food processing plants ; Food-Processing Industry - standards ; Fundamental and applied biological sciences. Psychology ; Humans ; Infant ; Infant Formula ; infant formulas ; Infant, Newborn ; isolation ; microbial detection ; Phylogeny ; powdered foods ; Powdered infant formula (PIF) manufacture ; Prevalence ; processing stages ; Pulsed-field gel electrophoresis</subject><ispartof>International journal of food microbiology, 2007-05, Vol.116 (1), p.73-81</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-e782445269d4ad9dd274bfed487f419f2e7578395c1f585902066d808702eb093</citedby><cites>FETCH-LOGICAL-c460t-e782445269d4ad9dd274bfed487f419f2e7578395c1f585902066d808702eb093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168160507000591$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18676829$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17307267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mullane, N.R.</creatorcontrib><creatorcontrib>Whyte, P.</creatorcontrib><creatorcontrib>Wall, P.G.</creatorcontrib><creatorcontrib>Quinn, T.</creatorcontrib><creatorcontrib>Fanning, S.</creatorcontrib><title>Application of pulsed-field gel electrophoresis to characterise and trace the prevalence of Enterobacter sakazakii in an infant formula processing facility</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Enterobacter sakazakii (
E. sakazakii) contamination of powdered infant formula (PIF) and its processing environment was monitored between April 2005 and March 2006. The purpose of the monitoring programme was to locate points of contamination, investigate clonal persistence, and identify possible dissemination routes along the processing chain. A total of 80
E. sakazakii isolates were recovered from the manufacturing facility. The overall frequency of isolation of
E. sakazakii in intermediate and final product was 2.5%, while specific locations in the processing environment were contaminated at frequencies up to 31%. All
E. sakazakii isolates were characterised by pulsed-field gel electrophoresis (PFGE).
XbaI macrorestriction digests yielded 19 unique pulse-types that could be grouped into 6 clusters of between 5 and 32 isolates. The formation of large clusters was consistent with the presence of a number of clones in the manufacturing environment. While the majority of isolates were of environmental origin (72.5%), no cluster was confined to one specific location and indistinguishable PFGE profiles were generated from isolates cultured from the manufacturing environment, sampling points along the processing chain and from intermediate and final product. These findings suggest that the manufacturing environment serves as a key route for sporadic contamination of PIF. These data will support the development of efficient intervention measures contributing to the reduction of
E. sakazakii in the PIF processing chain.</description><subject>bacterial contamination</subject><subject>Biological and medical sciences</subject><subject>Cluster Analysis</subject><subject>Colony Count, Microbial</subject><subject>Consumer Product Safety</subject><subject>Cronobacter sakazakii</subject><subject>Cronobacter sakazakii - classification</subject><subject>Cronobacter sakazakii - isolation & purification</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Enterobacter sakazakii</subject><subject>Environmental Microbiology</subject><subject>Equipment Contamination - prevention & control</subject><subject>food contamination</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>food pathogens</subject><subject>food processing plants</subject><subject>Food-Processing Industry - standards</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant Formula</subject><subject>infant formulas</subject><subject>Infant, Newborn</subject><subject>isolation</subject><subject>microbial detection</subject><subject>Phylogeny</subject><subject>powdered foods</subject><subject>Powdered infant formula (PIF) manufacture</subject><subject>Prevalence</subject><subject>processing stages</subject><subject>Pulsed-field gel electrophoresis</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQhyMEokvhFcAc4JYwdhLbOVar8keqxAF6trz2eNdbbxzspFJ5FV4Wb3elcuRia6Rvxj_PV1XvKTQUKP-0b_zexWgP3qTYMADeUNZAy59VKyrFULcdh-fVqrCyphz6i-pVznsA6NsWXlYXVLQgGBer6s_VNAVv9OzjSKIj0xIy2tp5DJZsMRAMaOYUp11MmH0mcyRmp5M2MyafkejRkrmUSOYdkinhvQ44lrIMux4LFDePLMn6Tv_Wd94TP5aucjo9zsTFdFiCLp3RYM5-3BKnjQ9-fnhdvXC6xHlzvi-r28_XP9df65vvX76tr25qU7451ygk67qe8cF22g7WMtFtHNpOCtfRwTEUvZDt0BvqetkPwIBzK0EKYLiBob2sPp7mlgy_FsyzOvhsMAQ9YlyyYiA72re8gMMJLGvPOaFTU_IHnR4UBXU0o_bqHzPqaEZRpuCx9-35kWVzQPvUeVZRgA9nQGejg0t6ND4_cZILLtkx7bsT53RUelskqNsfDGgLUIBeQCHWJwLL0u49JpWNPzqxPhWZykb_H4H_AmyivsY</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Mullane, N.R.</creator><creator>Whyte, P.</creator><creator>Wall, P.G.</creator><creator>Quinn, T.</creator><creator>Fanning, S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20070501</creationdate><title>Application of pulsed-field gel electrophoresis to characterise and trace the prevalence of Enterobacter sakazakii in an infant formula processing facility</title><author>Mullane, N.R. ; Whyte, P. ; Wall, P.G. ; Quinn, T. ; Fanning, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-e782445269d4ad9dd274bfed487f419f2e7578395c1f585902066d808702eb093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>bacterial contamination</topic><topic>Biological and medical sciences</topic><topic>Cluster Analysis</topic><topic>Colony Count, Microbial</topic><topic>Consumer Product Safety</topic><topic>Cronobacter sakazakii</topic><topic>Cronobacter sakazakii - classification</topic><topic>Cronobacter sakazakii - isolation & purification</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Enterobacter sakazakii</topic><topic>Environmental Microbiology</topic><topic>Equipment Contamination - prevention & control</topic><topic>food contamination</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>food pathogens</topic><topic>food processing plants</topic><topic>Food-Processing Industry - standards</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant Formula</topic><topic>infant formulas</topic><topic>Infant, Newborn</topic><topic>isolation</topic><topic>microbial detection</topic><topic>Phylogeny</topic><topic>powdered foods</topic><topic>Powdered infant formula (PIF) manufacture</topic><topic>Prevalence</topic><topic>processing stages</topic><topic>Pulsed-field gel electrophoresis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mullane, N.R.</creatorcontrib><creatorcontrib>Whyte, P.</creatorcontrib><creatorcontrib>Wall, P.G.</creatorcontrib><creatorcontrib>Quinn, T.</creatorcontrib><creatorcontrib>Fanning, S.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mullane, N.R.</au><au>Whyte, P.</au><au>Wall, P.G.</au><au>Quinn, T.</au><au>Fanning, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of pulsed-field gel electrophoresis to characterise and trace the prevalence of Enterobacter sakazakii in an infant formula processing facility</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>116</volume><issue>1</issue><spage>73</spage><epage>81</epage><pages>73-81</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>Enterobacter sakazakii (
E. sakazakii) contamination of powdered infant formula (PIF) and its processing environment was monitored between April 2005 and March 2006. The purpose of the monitoring programme was to locate points of contamination, investigate clonal persistence, and identify possible dissemination routes along the processing chain. A total of 80
E. sakazakii isolates were recovered from the manufacturing facility. The overall frequency of isolation of
E. sakazakii in intermediate and final product was 2.5%, while specific locations in the processing environment were contaminated at frequencies up to 31%. All
E. sakazakii isolates were characterised by pulsed-field gel electrophoresis (PFGE).
XbaI macrorestriction digests yielded 19 unique pulse-types that could be grouped into 6 clusters of between 5 and 32 isolates. The formation of large clusters was consistent with the presence of a number of clones in the manufacturing environment. While the majority of isolates were of environmental origin (72.5%), no cluster was confined to one specific location and indistinguishable PFGE profiles were generated from isolates cultured from the manufacturing environment, sampling points along the processing chain and from intermediate and final product. These findings suggest that the manufacturing environment serves as a key route for sporadic contamination of PIF. These data will support the development of efficient intervention measures contributing to the reduction of
E. sakazakii in the PIF processing chain.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17307267</pmid><doi>10.1016/j.ijfoodmicro.2006.12.036</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 2007-05, Vol.116 (1), p.73-81 |
| issn | 0168-1605 1879-3460 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | bacterial contamination Biological and medical sciences Cluster Analysis Colony Count, Microbial Consumer Product Safety Cronobacter sakazakii Cronobacter sakazakii - classification Cronobacter sakazakii - isolation & purification Electrophoresis, Gel, Pulsed-Field Enterobacter sakazakii Environmental Microbiology Equipment Contamination - prevention & control food contamination Food Contamination - analysis Food industries Food Microbiology food pathogens food processing plants Food-Processing Industry - standards Fundamental and applied biological sciences. Psychology Humans Infant Infant Formula infant formulas Infant, Newborn isolation microbial detection Phylogeny powdered foods Powdered infant formula (PIF) manufacture Prevalence processing stages Pulsed-field gel electrophoresis |
| title | Application of pulsed-field gel electrophoresis to characterise and trace the prevalence of Enterobacter sakazakii in an infant formula processing facility |
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