RAPD with microsatellite as a tool for differentiation of Candida genus yeasts isolated in brewing
Fifteen wild yeast strains were isolated in two factories of a lager brewing company in Poland. Their identification with API 32C system showed mainly the presence of Candida sake species (7/15). To differentiate the isolates, randomly amplified polymorphic DNA (RAPD) with (GTG)5, (GAC)5, (GACA)4 mi...
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| Veröffentlicht in: | Food microbiology 2007-05, Vol.24 (3), p.305-312 |
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| creator | Walczak, E Czaplinska, A Barszczewski, W Wilgosz, M Wojtatowicz, M Robak, M |
| description | Fifteen wild yeast strains were isolated in two factories of a lager brewing company in Poland. Their identification with API 32C system showed mainly the presence of Candida sake species (7/15). To differentiate the isolates, randomly amplified polymorphic DNA (RAPD) with (GTG)5, (GAC)5, (GACA)4 microsatellite primers and M13 core sequence (5'-GAG GGT GGC GGT TCT-3') were chosen. The results of patterns similarity are presented as dendrograms for each RAPD analysis and for overall patterns. On the overall patterns, all isolates identified as C. sake, except Strain No. 1, were regrouped in one cluster. Collection strain C. sake CBS 617 was similar in 46% to the cluster with six isolates (Strain Nos. 3, 6, 8, 11, 13, 14). The second reference strain C. sake CBS 159 and the Strain No. 1 were regrouped with other Candida species (collection strains) showing, respectively, only 20% and 42% of similarity to other C. sake strains. The similarity based on the overall dendrogram between isolate Nos. 3, 6, 8, 11, 13, 14 and C. sake CBS 617 was 49%. Between those strains and other Candida, the similarity was only 37%. |
| doi_str_mv | 10.1016/j.fm.2006.04.012 |
| format | Article |
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Their identification with API 32C system showed mainly the presence of Candida sake species (7/15). To differentiate the isolates, randomly amplified polymorphic DNA (RAPD) with (GTG)5, (GAC)5, (GACA)4 microsatellite primers and M13 core sequence (5'-GAG GGT GGC GGT TCT-3') were chosen. The results of patterns similarity are presented as dendrograms for each RAPD analysis and for overall patterns. On the overall patterns, all isolates identified as C. sake, except Strain No. 1, were regrouped in one cluster. Collection strain C. sake CBS 617 was similar in 46% to the cluster with six isolates (Strain Nos. 3, 6, 8, 11, 13, 14). The second reference strain C. sake CBS 159 and the Strain No. 1 were regrouped with other Candida species (collection strains) showing, respectively, only 20% and 42% of similarity to other C. sake strains. The similarity based on the overall dendrogram between isolate Nos. 3, 6, 8, 11, 13, 14 and C. sake CBS 617 was 49%. Between those strains and other Candida, the similarity was only 37%.</description><identifier>ISSN: 0740-0020</identifier><identifier>EISSN: 1095-9998</identifier><identifier>DOI: 10.1016/j.fm.2006.04.012</identifier><identifier>PMID: 17188210</identifier><identifier>CODEN: FOMIE5</identifier><language>eng</language><publisher>London: Elsevier</publisher><subject>Beer - microbiology ; Biological and medical sciences ; brewing industry ; Candida ; Candida - classification ; Candida - genetics ; Candida - isolation & purification ; Candida sake ; Cluster Analysis ; DNA, Fungal - analysis ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; isolation ; Microsatellite Repeats ; Phylogeny ; Poland ; polyploidy ; random amplified polymorphic DNA technique ; Random Amplified Polymorphic DNA Technique - methods ; serotypes ; Species Specificity ; starter cultures ; wild relatives ; yeasts</subject><ispartof>Food microbiology, 2007-05, Vol.24 (3), p.305-312</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-c814ceef44ef4781851cafcbaaef1fe447cce8d5721717640dee2038b69d66c63</citedby><cites>FETCH-LOGICAL-c382t-c814ceef44ef4781851cafcbaaef1fe447cce8d5721717640dee2038b69d66c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18481284$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17188210$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Walczak, E</creatorcontrib><creatorcontrib>Czaplinska, A</creatorcontrib><creatorcontrib>Barszczewski, W</creatorcontrib><creatorcontrib>Wilgosz, M</creatorcontrib><creatorcontrib>Wojtatowicz, M</creatorcontrib><creatorcontrib>Robak, M</creatorcontrib><title>RAPD with microsatellite as a tool for differentiation of Candida genus yeasts isolated in brewing</title><title>Food microbiology</title><addtitle>Food Microbiol</addtitle><description>Fifteen wild yeast strains were isolated in two factories of a lager brewing company in Poland. Their identification with API 32C system showed mainly the presence of Candida sake species (7/15). To differentiate the isolates, randomly amplified polymorphic DNA (RAPD) with (GTG)5, (GAC)5, (GACA)4 microsatellite primers and M13 core sequence (5'-GAG GGT GGC GGT TCT-3') were chosen. The results of patterns similarity are presented as dendrograms for each RAPD analysis and for overall patterns. On the overall patterns, all isolates identified as C. sake, except Strain No. 1, were regrouped in one cluster. Collection strain C. sake CBS 617 was similar in 46% to the cluster with six isolates (Strain Nos. 3, 6, 8, 11, 13, 14). The second reference strain C. sake CBS 159 and the Strain No. 1 were regrouped with other Candida species (collection strains) showing, respectively, only 20% and 42% of similarity to other C. sake strains. The similarity based on the overall dendrogram between isolate Nos. 3, 6, 8, 11, 13, 14 and C. sake CBS 617 was 49%. Between those strains and other Candida, the similarity was only 37%.</description><subject>Beer - microbiology</subject><subject>Biological and medical sciences</subject><subject>brewing industry</subject><subject>Candida</subject><subject>Candida - classification</subject><subject>Candida - genetics</subject><subject>Candida - isolation & purification</subject><subject>Candida sake</subject><subject>Cluster Analysis</subject><subject>DNA, Fungal - analysis</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>isolation</subject><subject>Microsatellite Repeats</subject><subject>Phylogeny</subject><subject>Poland</subject><subject>polyploidy</subject><subject>random amplified polymorphic DNA technique</subject><subject>Random Amplified Polymorphic DNA Technique - methods</subject><subject>serotypes</subject><subject>Species Specificity</subject><subject>starter cultures</subject><subject>wild relatives</subject><subject>yeasts</subject><issn>0740-0020</issn><issn>1095-9998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEFv1DAQRi1ERbeFOyfwBW5JZxxv4hyrLRSkSq2Ani3HGS9eJXGxvar67_FqV-phNJc3n-Z7jH1EqBGwvdrVbq4FQFuDrAHFG7ZC6NdV3_fqLVtBJ6ECEHDOLlLaASCum_4dO8cOlRIIKzb8un644c8-_-WztzEkk2mafCZuEjc8hzBxFyIfvXMUacneZB8WHhzfmGX0o-FbWvaJv5BJOXGfwlQiRu4XPkR69sv2PTtzZkr04bQv2eP3b382P6q7-9ufm-u7yjZK5MoqlJbISVmmU6jWaI2zgzHk0JGUnbWkxnUnyvddK2EkEtCooe3HtrVtc8m-HnOfYvi3p5T17JMtbcxCYZ-0ACWxlC4gHMFD3xTJ6afoZxNfNII-eNU77WZ98KpB6uK1nHw6Ze-HmcbXg5PIAnw5ASZZM7loFuvTK6ekQqFk4T4fOWeCNttYmMffArAB6JoepWj-A96QiwI</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Walczak, E</creator><creator>Czaplinska, A</creator><creator>Barszczewski, W</creator><creator>Wilgosz, M</creator><creator>Wojtatowicz, M</creator><creator>Robak, M</creator><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20070501</creationdate><title>RAPD with microsatellite as a tool for differentiation of Candida genus yeasts isolated in brewing</title><author>Walczak, E ; Czaplinska, A ; Barszczewski, W ; Wilgosz, M ; Wojtatowicz, M ; Robak, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-c814ceef44ef4781851cafcbaaef1fe447cce8d5721717640dee2038b69d66c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Beer - microbiology</topic><topic>Biological and medical sciences</topic><topic>brewing industry</topic><topic>Candida</topic><topic>Candida - classification</topic><topic>Candida - genetics</topic><topic>Candida - isolation & purification</topic><topic>Candida sake</topic><topic>Cluster Analysis</topic><topic>DNA, Fungal - analysis</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>isolation</topic><topic>Microsatellite Repeats</topic><topic>Phylogeny</topic><topic>Poland</topic><topic>polyploidy</topic><topic>random amplified polymorphic DNA technique</topic><topic>Random Amplified Polymorphic DNA Technique - methods</topic><topic>serotypes</topic><topic>Species Specificity</topic><topic>starter cultures</topic><topic>wild relatives</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walczak, E</creatorcontrib><creatorcontrib>Czaplinska, A</creatorcontrib><creatorcontrib>Barszczewski, W</creatorcontrib><creatorcontrib>Wilgosz, M</creatorcontrib><creatorcontrib>Wojtatowicz, M</creatorcontrib><creatorcontrib>Robak, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walczak, E</au><au>Czaplinska, A</au><au>Barszczewski, W</au><au>Wilgosz, M</au><au>Wojtatowicz, M</au><au>Robak, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RAPD with microsatellite as a tool for differentiation of Candida genus yeasts isolated in brewing</atitle><jtitle>Food microbiology</jtitle><addtitle>Food Microbiol</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>24</volume><issue>3</issue><spage>305</spage><epage>312</epage><pages>305-312</pages><issn>0740-0020</issn><eissn>1095-9998</eissn><coden>FOMIE5</coden><abstract>Fifteen wild yeast strains were isolated in two factories of a lager brewing company in Poland. Their identification with API 32C system showed mainly the presence of Candida sake species (7/15). To differentiate the isolates, randomly amplified polymorphic DNA (RAPD) with (GTG)5, (GAC)5, (GACA)4 microsatellite primers and M13 core sequence (5'-GAG GGT GGC GGT TCT-3') were chosen. The results of patterns similarity are presented as dendrograms for each RAPD analysis and for overall patterns. On the overall patterns, all isolates identified as C. sake, except Strain No. 1, were regrouped in one cluster. Collection strain C. sake CBS 617 was similar in 46% to the cluster with six isolates (Strain Nos. 3, 6, 8, 11, 13, 14). The second reference strain C. sake CBS 159 and the Strain No. 1 were regrouped with other Candida species (collection strains) showing, respectively, only 20% and 42% of similarity to other C. sake strains. The similarity based on the overall dendrogram between isolate Nos. 3, 6, 8, 11, 13, 14 and C. sake CBS 617 was 49%. Between those strains and other Candida, the similarity was only 37%.</abstract><cop>London</cop><pub>Elsevier</pub><pmid>17188210</pmid><doi>10.1016/j.fm.2006.04.012</doi><tpages>8</tpages></addata></record> |
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| subjects | Beer - microbiology Biological and medical sciences brewing industry Candida Candida - classification Candida - genetics Candida - isolation & purification Candida sake Cluster Analysis DNA, Fungal - analysis Food industries Food Microbiology Fundamental and applied biological sciences. Psychology isolation Microsatellite Repeats Phylogeny Poland polyploidy random amplified polymorphic DNA technique Random Amplified Polymorphic DNA Technique - methods serotypes Species Specificity starter cultures wild relatives yeasts |
| title | RAPD with microsatellite as a tool for differentiation of Candida genus yeasts isolated in brewing |
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