An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine
Ochratoxigenic mycobiota in grapes from representative wine regions in Valencia was identified. Black aspergilli were predominant among the different Aspergillus spp. isolated. Restriction digestion analysis of the ITS products was tested as a rapid method to identify isolates of black Aspergillus s...
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| Veröffentlicht in: | International journal of food microbiology 2007-01, Vol.113 (2), p.147-153 |
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| creator | Martinez-Culebras, P V Ramon, D |
| description | Ochratoxigenic mycobiota in grapes from representative wine regions in Valencia was identified. Black aspergilli were predominant among the different
Aspergillus spp. isolated. Restriction digestion analysis of the ITS products was tested as a rapid method to identify isolates of black
Aspergillus species from grapes. Restriction endonuclease digestion of the ITS products using the endonucleases
HhaI,
NlaIII and
RsaI, distinguished five types of restriction fragment length polymorphism (RFLP) corresponding to
Aspergillus niger,
Aspergillus tubingensis,
Aspergillus carbonarius and
Aspergillus aculeatus species. In addition, a new RFLP type in the
A. niger aggregate was identified. The fragments obtained by digestion with the endonuclease
NlaIII could be used to identify these new isolates. Black
Aspergillus isolates were tested for their ability to produce OTA. Most of the isolates that produced ochratoxin A in YES medium belonged to
A. carbonarius species. These results support evidence that
A. carbonarious greatly contributes to OTA contamination in grapes and consequently in wine. The ITS-RFLP assay is proposed as a rapid and easy method to identify black
Aspergillus species isolated from grapes, especially in studies that involve a large number of isolates. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2006.06.023 |
| format | Article |
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Aspergillus spp. isolated. Restriction digestion analysis of the ITS products was tested as a rapid method to identify isolates of black
Aspergillus species from grapes. Restriction endonuclease digestion of the ITS products using the endonucleases
HhaI,
NlaIII and
RsaI, distinguished five types of restriction fragment length polymorphism (RFLP) corresponding to
Aspergillus niger,
Aspergillus tubingensis,
Aspergillus carbonarius and
Aspergillus aculeatus species. In addition, a new RFLP type in the
A. niger aggregate was identified. The fragments obtained by digestion with the endonuclease
NlaIII could be used to identify these new isolates. Black
Aspergillus isolates were tested for their ability to produce OTA. Most of the isolates that produced ochratoxin A in YES medium belonged to
A. carbonarius species. These results support evidence that
A. carbonarious greatly contributes to OTA contamination in grapes and consequently in wine. The ITS-RFLP assay is proposed as a rapid and easy method to identify black
Aspergillus species isolated from grapes, especially in studies that involve a large number of isolates.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2006.06.023</identifier><identifier>PMID: 16870292</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Aspergillus - isolation & purification ; Aspergillus - metabolism ; Aspergillus aculeatus ; Aspergillus carbonarius ; Aspergillus niger ; Aspergillus tubingensis ; Biological and medical sciences ; Black aspergilli ; DNA Restriction Enzymes ; Fermented food industries ; Food Contamination - analysis ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Grapes ; Identification ; ITS ; Ochratoxins - analysis ; Ochratoxins - biosynthesis ; Polymorphism, Restriction Fragment Length ; RFLP analysis ; Vitaceae ; Vitis - chemistry ; Vitis - microbiology ; Wine ; Wine - analysis ; Wine - microbiology ; Wines and vinegars</subject><ispartof>International journal of food microbiology, 2007-01, Vol.113 (2), p.147-153</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-6a5022242a32b0d1a2821eb3aa4fe36a001d78095a0a6c56fe9aa02225a9b7493</citedby><cites>FETCH-LOGICAL-c436t-6a5022242a32b0d1a2821eb3aa4fe36a001d78095a0a6c56fe9aa02225a9b7493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168160506003382$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18419049$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16870292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martinez-Culebras, P V</creatorcontrib><creatorcontrib>Ramon, D</creatorcontrib><title>An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Ochratoxigenic mycobiota in grapes from representative wine regions in Valencia was identified. Black aspergilli were predominant among the different
Aspergillus spp. isolated. Restriction digestion analysis of the ITS products was tested as a rapid method to identify isolates of black
Aspergillus species from grapes. Restriction endonuclease digestion of the ITS products using the endonucleases
HhaI,
NlaIII and
RsaI, distinguished five types of restriction fragment length polymorphism (RFLP) corresponding to
Aspergillus niger,
Aspergillus tubingensis,
Aspergillus carbonarius and
Aspergillus aculeatus species. In addition, a new RFLP type in the
A. niger aggregate was identified. The fragments obtained by digestion with the endonuclease
NlaIII could be used to identify these new isolates. Black
Aspergillus isolates were tested for their ability to produce OTA. Most of the isolates that produced ochratoxin A in YES medium belonged to
A. carbonarius species. These results support evidence that
A. carbonarious greatly contributes to OTA contamination in grapes and consequently in wine. The ITS-RFLP assay is proposed as a rapid and easy method to identify black
Aspergillus species isolated from grapes, especially in studies that involve a large number of isolates.</description><subject>Aspergillus - isolation & purification</subject><subject>Aspergillus - metabolism</subject><subject>Aspergillus aculeatus</subject><subject>Aspergillus carbonarius</subject><subject>Aspergillus niger</subject><subject>Aspergillus tubingensis</subject><subject>Biological and medical sciences</subject><subject>Black aspergilli</subject><subject>DNA Restriction Enzymes</subject><subject>Fermented food industries</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Grapes</subject><subject>Identification</subject><subject>ITS</subject><subject>Ochratoxins - analysis</subject><subject>Ochratoxins - biosynthesis</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>RFLP analysis</subject><subject>Vitaceae</subject><subject>Vitis - chemistry</subject><subject>Vitis - microbiology</subject><subject>Wine</subject><subject>Wine - analysis</subject><subject>Wine - microbiology</subject><subject>Wines and vinegars</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFGL1DAQx4N4eOvpV5D4oG9dJ0mbto_L4unBwsnd3nOYptMza5vUpKvct7d1F85HYWBg-P1nhh9j7wWsBQj96bB2hy6EdnA2hrUE0OulpHrBVqIq60zlGl6y1cxWmdBQXLLXKR0AoFAKXrHLeV6CrOWK-Y3nN_v77O56940PNH0PLZ8Cdy35yXVPvOnR_uCbNFJ8dH1_TNyl0ONEiUdKY_DJNT3xLkR-u99wG_yEg_M4ueC58_wx4jiz6Fv-23l6wy467BO9Pfcr9nD9eb_9mu1uv9xsN7vM5kpPmcYCpJS5RCUbaAXKSgpqFGLekdIIINqygrpAQG0L3VGNuCQKrJsyr9UV-3jaO8bw80hpMoNLlvoePYVjMhIqVepSzmB9AmeTKUXqzBjdgPHJCDCLbHMw_8g2i2yzlFRz9t35yLEZqH1Onu3OwIczgMli30X01qVnrspFDX-_3Z44mpX8chRNso68pdZFspNpg_uPd_4AwRGjcA</recordid><startdate>20070125</startdate><enddate>20070125</enddate><creator>Martinez-Culebras, P V</creator><creator>Ramon, D</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20070125</creationdate><title>An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine</title><author>Martinez-Culebras, P V ; Ramon, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-6a5022242a32b0d1a2821eb3aa4fe36a001d78095a0a6c56fe9aa02225a9b7493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Aspergillus - isolation & purification</topic><topic>Aspergillus - metabolism</topic><topic>Aspergillus aculeatus</topic><topic>Aspergillus carbonarius</topic><topic>Aspergillus niger</topic><topic>Aspergillus tubingensis</topic><topic>Biological and medical sciences</topic><topic>Black aspergilli</topic><topic>DNA Restriction Enzymes</topic><topic>Fermented food industries</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Grapes</topic><topic>Identification</topic><topic>ITS</topic><topic>Ochratoxins - analysis</topic><topic>Ochratoxins - biosynthesis</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>RFLP analysis</topic><topic>Vitaceae</topic><topic>Vitis - chemistry</topic><topic>Vitis - microbiology</topic><topic>Wine</topic><topic>Wine - analysis</topic><topic>Wine - microbiology</topic><topic>Wines and vinegars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martinez-Culebras, P V</creatorcontrib><creatorcontrib>Ramon, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martinez-Culebras, P V</au><au>Ramon, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2007-01-25</date><risdate>2007</risdate><volume>113</volume><issue>2</issue><spage>147</spage><epage>153</epage><pages>147-153</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>Ochratoxigenic mycobiota in grapes from representative wine regions in Valencia was identified. Black aspergilli were predominant among the different
Aspergillus spp. isolated. Restriction digestion analysis of the ITS products was tested as a rapid method to identify isolates of black
Aspergillus species from grapes. Restriction endonuclease digestion of the ITS products using the endonucleases
HhaI,
NlaIII and
RsaI, distinguished five types of restriction fragment length polymorphism (RFLP) corresponding to
Aspergillus niger,
Aspergillus tubingensis,
Aspergillus carbonarius and
Aspergillus aculeatus species. In addition, a new RFLP type in the
A. niger aggregate was identified. The fragments obtained by digestion with the endonuclease
NlaIII could be used to identify these new isolates. Black
Aspergillus isolates were tested for their ability to produce OTA. Most of the isolates that produced ochratoxin A in YES medium belonged to
A. carbonarius species. These results support evidence that
A. carbonarious greatly contributes to OTA contamination in grapes and consequently in wine. The ITS-RFLP assay is proposed as a rapid and easy method to identify black
Aspergillus species isolated from grapes, especially in studies that involve a large number of isolates.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16870292</pmid><doi>10.1016/j.ijfoodmicro.2006.06.023</doi><tpages>7</tpages></addata></record> |
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| subjects | Aspergillus - isolation & purification Aspergillus - metabolism Aspergillus aculeatus Aspergillus carbonarius Aspergillus niger Aspergillus tubingensis Biological and medical sciences Black aspergilli DNA Restriction Enzymes Fermented food industries Food Contamination - analysis Food industries Food Microbiology Fundamental and applied biological sciences. Psychology Grapes Identification ITS Ochratoxins - analysis Ochratoxins - biosynthesis Polymorphism, Restriction Fragment Length RFLP analysis Vitaceae Vitis - chemistry Vitis - microbiology Wine Wine - analysis Wine - microbiology Wines and vinegars |
| title | An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine |
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