Different amplification patterns of 3q26 and 5p15 regions in cervical intraepithelial neoplasia and cervical cancer
The aim of this study was to evaluate and correlate the amplification of chromosomal regions 3q26 and 5p15 in different cytological and histological subgroups of patients and to compare the sensitivity and specificity of amplification tests with cytology, colposcopy and HPV status. The work was cond...
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| Veröffentlicht in: | Annals of diagnostic pathology 2018-08, Vol.35, p.16-20 |
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| creator | Kudela, Erik Visnovsky, Jozef Balharek, Tomas Farkasova, Anna Zubor, Pavol Plank, Lukas Danko, Jan |
| description | The aim of this study was to evaluate and correlate the amplification of chromosomal regions 3q26 and 5p15 in different cytological and histological subgroups of patients and to compare the sensitivity and specificity of amplification tests with cytology, colposcopy and HPV status.
The work was conducted at the Department of Obstetrics and Gynaecology in cooperation with the Institute of Pathological Anatomy, JFM CU in Martin and UNM during years 2013–2016. Prospective longitudinal study included 131 patients. We focused on the FISH diagnosis of the amplification of regions encoding the components of telomerase enzyme (3q26, 5p15) in cytology specimens. We manually evaluated 100 atypical cells per slide and analysed the amplification patterns. Correlations between cytological, histological, HPV DNA results and amplification patterns of chromosomal regions 3q26 and 5p15 were analysed by chi-squared test and non-parametric Man - Whitney U test.
The results showed that the amplification of chromosomal regions increases with the degree of dysplasia toward the invasive disease (p |
| doi_str_mv | 10.1016/j.anndiagpath.2018.02.003 |
| format | Article |
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The work was conducted at the Department of Obstetrics and Gynaecology in cooperation with the Institute of Pathological Anatomy, JFM CU in Martin and UNM during years 2013–2016. Prospective longitudinal study included 131 patients. We focused on the FISH diagnosis of the amplification of regions encoding the components of telomerase enzyme (3q26, 5p15) in cytology specimens. We manually evaluated 100 atypical cells per slide and analysed the amplification patterns. Correlations between cytological, histological, HPV DNA results and amplification patterns of chromosomal regions 3q26 and 5p15 were analysed by chi-squared test and non-parametric Man - Whitney U test.
The results showed that the amplification of chromosomal regions increases with the degree of dysplasia toward the invasive disease (p < 0.001). Whereas the increase in the amplification of 3q26 is noticeable already at CIN 2 + lesions (p < 0.01), 5p15 amplification is shifted up toward CIN 3/CIS (p < 0.001) and cervical cancer. Amplification of selected regions correlated with each other and also with hrHPV-positive status (p < 0.01).
The analysis of the amplification of 3q26 and 5p15 regions may serve in the future for the differential diagnosis of cervical lesions and to determine their malignant potential. High specificity of these tests can improve the excellent sensitivity of HPV DNA test.
•We focused on amplification patterns of chromosomal regions 3q26 and 5p15 connected with the telomerase activity.•Telomerase activity could be a valuable marker for the diagnosis of cervical neoplasia.•The amplification of 3q26 and 5p15 regions may serve in the future for the differential diagnosis of cervical lesions.</description><identifier>ISSN: 1092-9134</identifier><identifier>EISSN: 1532-8198</identifier><identifier>DOI: 10.1016/j.anndiagpath.2018.02.003</identifier><identifier>PMID: 30072014</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Amplification ; Cervical intraepithelial neoplasia ; Cervical Intraepithelial Neoplasia - genetics ; Cervical Intraepithelial Neoplasia - pathology ; Chromosomes, Human, Pair 3 ; Chromosomes, Human, Pair 5 ; Diagnosis, Differential ; Female ; Fluorescent in situ hybridization ; Gene Amplification ; Humans ; Middle Aged ; Prospective Studies ; Sensitivity and Specificity ; Telomerase ; TERC ; TERT ; Uterine Cervical Neoplasms - genetics ; Uterine Cervical Neoplasms - pathology</subject><ispartof>Annals of diagnostic pathology, 2018-08, Vol.35, p.16-20</ispartof><rights>2018 Elsevier Inc.</rights><rights>Copyright © 2018 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-fc3d04b4d10afdf7a4d564db44720ad89441bcbc2fb282afb24440686a2ec8763</citedby><cites>FETCH-LOGICAL-c443t-fc3d04b4d10afdf7a4d564db44720ad89441bcbc2fb282afb24440686a2ec8763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1092913418300030$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30072014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kudela, Erik</creatorcontrib><creatorcontrib>Visnovsky, Jozef</creatorcontrib><creatorcontrib>Balharek, Tomas</creatorcontrib><creatorcontrib>Farkasova, Anna</creatorcontrib><creatorcontrib>Zubor, Pavol</creatorcontrib><creatorcontrib>Plank, Lukas</creatorcontrib><creatorcontrib>Danko, Jan</creatorcontrib><title>Different amplification patterns of 3q26 and 5p15 regions in cervical intraepithelial neoplasia and cervical cancer</title><title>Annals of diagnostic pathology</title><addtitle>Ann Diagn Pathol</addtitle><description>The aim of this study was to evaluate and correlate the amplification of chromosomal regions 3q26 and 5p15 in different cytological and histological subgroups of patients and to compare the sensitivity and specificity of amplification tests with cytology, colposcopy and HPV status.
The work was conducted at the Department of Obstetrics and Gynaecology in cooperation with the Institute of Pathological Anatomy, JFM CU in Martin and UNM during years 2013–2016. Prospective longitudinal study included 131 patients. We focused on the FISH diagnosis of the amplification of regions encoding the components of telomerase enzyme (3q26, 5p15) in cytology specimens. We manually evaluated 100 atypical cells per slide and analysed the amplification patterns. Correlations between cytological, histological, HPV DNA results and amplification patterns of chromosomal regions 3q26 and 5p15 were analysed by chi-squared test and non-parametric Man - Whitney U test.
The results showed that the amplification of chromosomal regions increases with the degree of dysplasia toward the invasive disease (p < 0.001). Whereas the increase in the amplification of 3q26 is noticeable already at CIN 2 + lesions (p < 0.01), 5p15 amplification is shifted up toward CIN 3/CIS (p < 0.001) and cervical cancer. Amplification of selected regions correlated with each other and also with hrHPV-positive status (p < 0.01).
The analysis of the amplification of 3q26 and 5p15 regions may serve in the future for the differential diagnosis of cervical lesions and to determine their malignant potential. High specificity of these tests can improve the excellent sensitivity of HPV DNA test.
•We focused on amplification patterns of chromosomal regions 3q26 and 5p15 connected with the telomerase activity.•Telomerase activity could be a valuable marker for the diagnosis of cervical neoplasia.•The amplification of 3q26 and 5p15 regions may serve in the future for the differential diagnosis of cervical lesions.</description><subject>Adult</subject><subject>Amplification</subject><subject>Cervical intraepithelial neoplasia</subject><subject>Cervical Intraepithelial Neoplasia - genetics</subject><subject>Cervical Intraepithelial Neoplasia - pathology</subject><subject>Chromosomes, Human, Pair 3</subject><subject>Chromosomes, Human, Pair 5</subject><subject>Diagnosis, Differential</subject><subject>Female</subject><subject>Fluorescent in situ hybridization</subject><subject>Gene Amplification</subject><subject>Humans</subject><subject>Middle Aged</subject><subject>Prospective Studies</subject><subject>Sensitivity and Specificity</subject><subject>Telomerase</subject><subject>TERC</subject><subject>TERT</subject><subject>Uterine Cervical Neoplasms - genetics</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>1092-9134</issn><issn>1532-8198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtuFDEQRS0EIg_4BWR2bLopP_q1REMgSJHYwNqqtsuJRz3uju2JxN_jMCFiycYuS-dWlQ9j7wW0AkT_cd9ijC7g7YblrpUgxhZkC6BesHPRKdmMYhpf1hom2UxC6TN2kfMeQAjdDa_ZmQIYakyfs_w5eE-JYuF42Jbgg8US1shr60IpZr56ru5lzzE63m2i44luK5B5iNxSeqiBpdYlIW2h3NES6jvSui2YA_6JPWMWY63fsFcel0xvn-5L9vPL1Y_ddXPz_eu33aebxmqtSuOtcqBn7QSgd35A7bpeu1nruju6cdJazHa20s9ylFhPrTX0Y4-S7Dj06pJ9OPXd0np_pFzMIWRLy4J1vWM2EkY1SN11UNHphNq05pzImy2FA6ZfRoB5dG725h_n5tG5AWmq85p99zTmOB_IPSf_Sq7A7gRQ_exDoGSyDVRNuJDIFuPW8B9jfgMpfZn1</recordid><startdate>201808</startdate><enddate>201808</enddate><creator>Kudela, Erik</creator><creator>Visnovsky, Jozef</creator><creator>Balharek, Tomas</creator><creator>Farkasova, Anna</creator><creator>Zubor, Pavol</creator><creator>Plank, Lukas</creator><creator>Danko, Jan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201808</creationdate><title>Different amplification patterns of 3q26 and 5p15 regions in cervical intraepithelial neoplasia and cervical cancer</title><author>Kudela, Erik ; Visnovsky, Jozef ; Balharek, Tomas ; Farkasova, Anna ; Zubor, Pavol ; Plank, Lukas ; Danko, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-fc3d04b4d10afdf7a4d564db44720ad89441bcbc2fb282afb24440686a2ec8763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adult</topic><topic>Amplification</topic><topic>Cervical intraepithelial neoplasia</topic><topic>Cervical Intraepithelial Neoplasia - genetics</topic><topic>Cervical Intraepithelial Neoplasia - pathology</topic><topic>Chromosomes, Human, Pair 3</topic><topic>Chromosomes, Human, Pair 5</topic><topic>Diagnosis, Differential</topic><topic>Female</topic><topic>Fluorescent in situ hybridization</topic><topic>Gene Amplification</topic><topic>Humans</topic><topic>Middle Aged</topic><topic>Prospective Studies</topic><topic>Sensitivity and Specificity</topic><topic>Telomerase</topic><topic>TERC</topic><topic>TERT</topic><topic>Uterine Cervical Neoplasms - genetics</topic><topic>Uterine Cervical Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kudela, Erik</creatorcontrib><creatorcontrib>Visnovsky, Jozef</creatorcontrib><creatorcontrib>Balharek, Tomas</creatorcontrib><creatorcontrib>Farkasova, Anna</creatorcontrib><creatorcontrib>Zubor, Pavol</creatorcontrib><creatorcontrib>Plank, Lukas</creatorcontrib><creatorcontrib>Danko, Jan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of diagnostic pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kudela, Erik</au><au>Visnovsky, Jozef</au><au>Balharek, Tomas</au><au>Farkasova, Anna</au><au>Zubor, Pavol</au><au>Plank, Lukas</au><au>Danko, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Different amplification patterns of 3q26 and 5p15 regions in cervical intraepithelial neoplasia and cervical cancer</atitle><jtitle>Annals of diagnostic pathology</jtitle><addtitle>Ann Diagn Pathol</addtitle><date>2018-08</date><risdate>2018</risdate><volume>35</volume><spage>16</spage><epage>20</epage><pages>16-20</pages><issn>1092-9134</issn><eissn>1532-8198</eissn><abstract>The aim of this study was to evaluate and correlate the amplification of chromosomal regions 3q26 and 5p15 in different cytological and histological subgroups of patients and to compare the sensitivity and specificity of amplification tests with cytology, colposcopy and HPV status.
The work was conducted at the Department of Obstetrics and Gynaecology in cooperation with the Institute of Pathological Anatomy, JFM CU in Martin and UNM during years 2013–2016. Prospective longitudinal study included 131 patients. We focused on the FISH diagnosis of the amplification of regions encoding the components of telomerase enzyme (3q26, 5p15) in cytology specimens. We manually evaluated 100 atypical cells per slide and analysed the amplification patterns. Correlations between cytological, histological, HPV DNA results and amplification patterns of chromosomal regions 3q26 and 5p15 were analysed by chi-squared test and non-parametric Man - Whitney U test.
The results showed that the amplification of chromosomal regions increases with the degree of dysplasia toward the invasive disease (p < 0.001). Whereas the increase in the amplification of 3q26 is noticeable already at CIN 2 + lesions (p < 0.01), 5p15 amplification is shifted up toward CIN 3/CIS (p < 0.001) and cervical cancer. Amplification of selected regions correlated with each other and also with hrHPV-positive status (p < 0.01).
The analysis of the amplification of 3q26 and 5p15 regions may serve in the future for the differential diagnosis of cervical lesions and to determine their malignant potential. High specificity of these tests can improve the excellent sensitivity of HPV DNA test.
•We focused on amplification patterns of chromosomal regions 3q26 and 5p15 connected with the telomerase activity.•Telomerase activity could be a valuable marker for the diagnosis of cervical neoplasia.•The amplification of 3q26 and 5p15 regions may serve in the future for the differential diagnosis of cervical lesions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>30072014</pmid><doi>10.1016/j.anndiagpath.2018.02.003</doi><tpages>5</tpages></addata></record> |
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| subjects | Adult Amplification Cervical intraepithelial neoplasia Cervical Intraepithelial Neoplasia - genetics Cervical Intraepithelial Neoplasia - pathology Chromosomes, Human, Pair 3 Chromosomes, Human, Pair 5 Diagnosis, Differential Female Fluorescent in situ hybridization Gene Amplification Humans Middle Aged Prospective Studies Sensitivity and Specificity Telomerase TERC TERT Uterine Cervical Neoplasms - genetics Uterine Cervical Neoplasms - pathology |
| title | Different amplification patterns of 3q26 and 5p15 regions in cervical intraepithelial neoplasia and cervical cancer |
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