Novel vacuum stable ketone‐based matrices for high spatial resolution MALDI imaging mass spectrometry
We describe the use of aromatic ketones and cinnamyl ketones that have high vacuum stability for analyzing tissue sections using matrix‐assisted laser desorption/ionization imaging mass spectrometry. Specifically, the matrix, (E)‐4‐(2,5‐dihydroxyphenyl)but‐3‐en‐2‐one (2,5‐cDHA) provides high sensiti...
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| Veröffentlicht in: | Journal of mass spectrometry. 2018-10, Vol.53 (10), p.1005-1012 |
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| container_title | Journal of mass spectrometry. |
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| creator | Yang, Junhai Norris, Jeremy L. Caprioli, Richard |
| description | We describe the use of aromatic ketones and cinnamyl ketones that have high vacuum stability for analyzing tissue sections using matrix‐assisted laser desorption/ionization imaging mass spectrometry. Specifically, the matrix, (E)‐4‐(2,5‐dihydroxyphenyl)but‐3‐en‐2‐one (2,5‐cDHA) provides high sensitivity and high vacuum stability while producing small size crystals (1‐2 μm). A high throughput and highly reproducible sample preparation method was developed for these matrices that first involves using an organic spray solution for small matrix crystal seeding followed by spraying of the matrix in a 30% acetonitrile/70% water solution on the tissue surface to obtain a homogeneous coating of small crystals, suitable for high spatial resolution imaging. |
| doi_str_mv | 10.1002/jms.4277 |
| format | Article |
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Specifically, the matrix, (E)‐4‐(2,5‐dihydroxyphenyl)but‐3‐en‐2‐one (2,5‐cDHA) provides high sensitivity and high vacuum stability while producing small size crystals (1‐2 μm). A high throughput and highly reproducible sample preparation method was developed for these matrices that first involves using an organic spray solution for small matrix crystal seeding followed by spraying of the matrix in a 30% acetonitrile/70% water solution on the tissue surface to obtain a homogeneous coating of small crystals, suitable for high spatial resolution imaging.</description><identifier>ISSN: 1076-5174</identifier><identifier>EISSN: 1096-9888</identifier><identifier>DOI: 10.1002/jms.4277</identifier><identifier>PMID: 30073737</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Acetonitrile ; Aromatic compounds ; Crystals ; Desorption ; high spatial resolution ; High vacuum ; imaging ; Imaging techniques ; Ionization ; Ions ; Ketones ; Lasers ; MALDI ; Mass spectrometry ; Mass spectroscopy ; matrix ; Resolution ; Sample preparation ; Scientific imaging ; Spatial discrimination ; Spatial resolution ; Spectroscopy ; Spraying ; Stability ; Stability analysis ; Tissues ; Vacuum</subject><ispartof>Journal of mass spectrometry., 2018-10, Vol.53 (10), p.1005-1012</ispartof><rights>2018 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3867-ad09d0df1ad1af05097c6382b4a16acb02af2c5e856c2985359b2d628239ed923</citedby><cites>FETCH-LOGICAL-c3867-ad09d0df1ad1af05097c6382b4a16acb02af2c5e856c2985359b2d628239ed923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjms.4277$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjms.4277$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30073737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Junhai</creatorcontrib><creatorcontrib>Norris, Jeremy L.</creatorcontrib><creatorcontrib>Caprioli, Richard</creatorcontrib><title>Novel vacuum stable ketone‐based matrices for high spatial resolution MALDI imaging mass spectrometry</title><title>Journal of mass spectrometry.</title><addtitle>J Mass Spectrom</addtitle><description>We describe the use of aromatic ketones and cinnamyl ketones that have high vacuum stability for analyzing tissue sections using matrix‐assisted laser desorption/ionization imaging mass spectrometry. Specifically, the matrix, (E)‐4‐(2,5‐dihydroxyphenyl)but‐3‐en‐2‐one (2,5‐cDHA) provides high sensitivity and high vacuum stability while producing small size crystals (1‐2 μm). A high throughput and highly reproducible sample preparation method was developed for these matrices that first involves using an organic spray solution for small matrix crystal seeding followed by spraying of the matrix in a 30% acetonitrile/70% water solution on the tissue surface to obtain a homogeneous coating of small crystals, suitable for high spatial resolution imaging.</description><subject>Acetonitrile</subject><subject>Aromatic compounds</subject><subject>Crystals</subject><subject>Desorption</subject><subject>high spatial resolution</subject><subject>High vacuum</subject><subject>imaging</subject><subject>Imaging techniques</subject><subject>Ionization</subject><subject>Ions</subject><subject>Ketones</subject><subject>Lasers</subject><subject>MALDI</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>matrix</subject><subject>Resolution</subject><subject>Sample preparation</subject><subject>Scientific imaging</subject><subject>Spatial discrimination</subject><subject>Spatial resolution</subject><subject>Spectroscopy</subject><subject>Spraying</subject><subject>Stability</subject><subject>Stability analysis</subject><subject>Tissues</subject><subject>Vacuum</subject><issn>1076-5174</issn><issn>1096-9888</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kM9O3DAQhy1UVGBbiSdAlrhwCYztTWwfES0UtLSHtmfLcSZLliRe7AS0Nx6hz9gnqZflj4RUzWHm8M2nmR8h-wyOGQA_WXTxeMql3CK7DHSRaaXUh_UsiyxncrpD9mJcAIDW0-Ij2REAUqTaJfPv_h5bem_dOHY0DrZskd7i4Hv8-_intBEr2tkhNA4jrX2gN838hsalHRrb0oDRt-PQ-J5en86-XNKms_Omn6eVGBOFbgi-wyGsPpHt2rYRPz_3Cfl9_vXX2bds9uPi8ux0ljmhCpnZCnQFVc1sxWwNOWjpCqF4ObWssK4EbmvuclR54bhWuch1yauCKy40VpqLCTnaeJfB340YB9M10WHb2h79GA0HJSQTuYKEHr5DF34MfbrOcMak0MBz9SZ0wccYsDbLkL4MK8PArMM3KXyzDj-hB8_CseywegVf0k5AtgEemhZX_xWZq-ufT8J_Q26Oyw</recordid><startdate>201810</startdate><enddate>201810</enddate><creator>Yang, Junhai</creator><creator>Norris, Jeremy L.</creator><creator>Caprioli, Richard</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H97</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L.G</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201810</creationdate><title>Novel vacuum stable ketone‐based matrices for high spatial resolution MALDI imaging mass spectrometry</title><author>Yang, Junhai ; Norris, Jeremy L. ; Caprioli, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3867-ad09d0df1ad1af05097c6382b4a16acb02af2c5e856c2985359b2d628239ed923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acetonitrile</topic><topic>Aromatic compounds</topic><topic>Crystals</topic><topic>Desorption</topic><topic>high spatial resolution</topic><topic>High vacuum</topic><topic>imaging</topic><topic>Imaging techniques</topic><topic>Ionization</topic><topic>Ions</topic><topic>Ketones</topic><topic>Lasers</topic><topic>MALDI</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>matrix</topic><topic>Resolution</topic><topic>Sample preparation</topic><topic>Scientific imaging</topic><topic>Spatial discrimination</topic><topic>Spatial resolution</topic><topic>Spectroscopy</topic><topic>Spraying</topic><topic>Stability</topic><topic>Stability analysis</topic><topic>Tissues</topic><topic>Vacuum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Junhai</creatorcontrib><creatorcontrib>Norris, Jeremy L.</creatorcontrib><creatorcontrib>Caprioli, Richard</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of mass spectrometry.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Junhai</au><au>Norris, Jeremy L.</au><au>Caprioli, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel vacuum stable ketone‐based matrices for high spatial resolution MALDI imaging mass spectrometry</atitle><jtitle>Journal of mass spectrometry.</jtitle><addtitle>J Mass Spectrom</addtitle><date>2018-10</date><risdate>2018</risdate><volume>53</volume><issue>10</issue><spage>1005</spage><epage>1012</epage><pages>1005-1012</pages><issn>1076-5174</issn><eissn>1096-9888</eissn><abstract>We describe the use of aromatic ketones and cinnamyl ketones that have high vacuum stability for analyzing tissue sections using matrix‐assisted laser desorption/ionization imaging mass spectrometry. Specifically, the matrix, (E)‐4‐(2,5‐dihydroxyphenyl)but‐3‐en‐2‐one (2,5‐cDHA) provides high sensitivity and high vacuum stability while producing small size crystals (1‐2 μm). A high throughput and highly reproducible sample preparation method was developed for these matrices that first involves using an organic spray solution for small matrix crystal seeding followed by spraying of the matrix in a 30% acetonitrile/70% water solution on the tissue surface to obtain a homogeneous coating of small crystals, suitable for high spatial resolution imaging.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30073737</pmid><doi>10.1002/jms.4277</doi><tpages>8</tpages></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Acetonitrile Aromatic compounds Crystals Desorption high spatial resolution High vacuum imaging Imaging techniques Ionization Ions Ketones Lasers MALDI Mass spectrometry Mass spectroscopy matrix Resolution Sample preparation Scientific imaging Spatial discrimination Spatial resolution Spectroscopy Spraying Stability Stability analysis Tissues Vacuum |
| title | Novel vacuum stable ketone‐based matrices for high spatial resolution MALDI imaging mass spectrometry |
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