Engineering enzymes for improved catalytic efficiency: a computational study of site mutagenesis in epothilone-B hydroxylase

Epothilone F, 21-hydroxyl-epothilone B, is an intermediate in the synthesis of BMS-310705, an antitumor compound that has been evaluated in Phase I clinical trials. A bioconversion process utilizing the Gram-positive bacterium Amycolatopsis orientalis was used to prepare epothilone F from epothilone...

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Veröffentlicht in:	Protein engineering, design and selection design and selection, 2009-04, Vol.22 (4), p.257-266
Hauptverfasser:	Nayeem, Akbar, Chiang, Shu-Jen, Liu, Suo-Win, Sun, Yuhua, You, Li, Basch, Jonathan
Format:	Artikel
Sprache:	eng
Schlagworte:	Amycolatopsis orientalis Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism biocatalysis Catalytic Domain - genetics Computer Simulation Cytochrome P-450 Enzyme System cytochrome P450 epothilone Epothilones - metabolism homology modeling Mixed Function Oxygenases - chemistry Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism Models, Molecular mutagenesis Mutagenesis, Site-Directed Protein Binding Protein Conformation Sequence Alignment Structural Homology, Protein
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