Replacement of Fish Meal with Poultry By-product Meal as a Protein Source in Pond-raised Sunshine Bass, Morone chrysops [female symbol] x M. saxatlis [male symbol], Diets

Replacement of fish meal (FM) as a protein source with alternative sources of protein in aquaculture diets has been widely explored in aquaculture. The goal of replacement of FM in production diets is to maintain growth, lower production costs, and increase sustainability. Evaluation of the replacement of FM with poultry by-product meal (PBM) in phase II sunshine bass diets, Morone chrysops x M. saxatilis, was conducted in ponds over 246 d. Four diets were formulated to be isonitrogenous (37%) and isocaloric (4 kcal/g) with different levels of FM replacement with PBM (0, 33, 67, and 100%, Diets 1-4, respectively). Twelve ponds were stocked with 400 phase II sunshine bass (mean weight 5.6 g) and randomly assigned one of the four diets. Fish were fed below satiation based on predicted growth and feed conversion, initially once daily (1700 h) and then twice daily (0700 and 1700 h) as water temperatures and feeding activity increased. Diets were evaluated based on production and performance indicators, body composition, and economic analysis. Production results revealed no significant differences in mean final individual fish weight (511 ± 21 g), net production (4257 ± 247 kg/ha), and survival (85 ± 2%). No significant differences occurred between the performance indicators: mean feed conversion ratio (2.47 ± 0.11), specific growth rate (1.84 ± 0.02), and protein conversion efficiency (23 ± 1.3%). Body composition was statistically similar for mean percent fillet weight (49 ± 0.6%) and percent intraperitoneal fat (9.8 ± 1.0%); however, the hepatosomatic index was significantly different between Diets 3 (3.7 ± 0.1%) and 4 (3.2 ± 0.1%). Mean proximate analysis of whole fish (dry weight basis) was not significantly different among treatments yielding the following: percent protein (46 ± 0.4%), lipid (47 ± 1.3%), and ash (8 ± 0.7%). Mean fillet composition (dry weight basis) also revealed no significant differences: percent protein (72 ± 0.8%), percent lipid (30 ± 1.6%), and percent ash (5 ± 0.2%). Proximate analysis was also performed on the diets and revealed a significantly lower protein content in Diet 3 (34.3 ± 0.5%) compared to the other diets (37.1 ± 0.4%). Amino acid analysis of the diets indicated a possible deficiency in methionine in Diets 3 and 4. Based on production, performance, and body composition, the results indicate that complete replacement of FM with PBM in sunshine bass diets is feasible; however, economic analysis suggests that the replaceme