Butanol fermentation research: upstream and downstream manipulations
An overview of advances in acetone–butanol fermentation research is presented with specific reference to the history of acetone–butanol fermentation, genetic manipulation of the butanol‐producing Clostridium beijerinckii NCIMB 8052, as well as upstream and downstream processing. Specific reference i...
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| Veröffentlicht in: | Chemical record 2004, Vol.4 (5), p.305-314 |
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| description | An overview of advances in acetone–butanol fermentation research is presented with specific reference to the history of acetone–butanol fermentation, genetic manipulation of the butanol‐producing Clostridium beijerinckii NCIMB 8052, as well as upstream and downstream processing. Specific reference is made to the development of the hyperamylolytic, hyper‐“butanolagenic” C. beijerinckii BA101 strain. Amylolytic enzyme production by C. beijerinckii BA101 was 1.8‐ and 2.5‐fold greater than that of the C. beijerinckii NCIMB 8052 strain grown in starch and glucose, respectively. We confirmed the presence of a phosphoenolpyruvate (PEP)‐dependent phosphotransferase system (PTS) associated with cell extracts of C. beijerinckii BA101 by glucose phosphorylation by PEP and ATP‐dependent glucose phosphorylation. It was found that C. beijerinckii BA101 was defective in PTS activity and that it compensates for this defect with enhanced glucokinase activity, resulting in an ability to transport and utilize glucose during the solventogenic stage. The principal problem associated with acetone–butanol fermentation by C. beijerinckii or C. acetobutylicum is butanol toxicity/inhibition to the culture. To solve this problem, we have attempted various alternative in situ/online techniques of butanol removal including membrane‐based systems such as pervaporation, liquid–liquid extraction, and gas stripping. We found that gas stripping and pervaporation appear to be the most promising of the in situ acetone–butanol fermentation and recovery techniques but, in terms of cost‐effective industrial applications, gas stripping appears to be the most promising. © 2004 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 4: 305–314; 2004: Published online in Wiley InterScience (www.interscience.wiley.com) DOI 10.1002/tcr.20023
An overview of advances in acetone–butanol fermentation research is presented with specific reference to the history of acetone–butanol fermentation, genetic manipulation of the butanol‐producing Clostridium beijerinckii NCIMB 8052, as well as upstream and downstream processing. Specific reference is made to the development of the hyperamylolytic, hyper‐“butanolagenic” C. beijerinckii BA101 strain. The principal problem associated with acetone–butanol fermentation by C. beijerinckii or C. acetobutylicum is butanol toxicity/inhibition to the culture. To solve this problem, we have attempted various alternative in situ/online techniques of butanol rem |
| doi_str_mv | 10.1002/tcr.20023 |
| format | Article |
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An overview of advances in acetone–butanol fermentation research is presented with specific reference to the history of acetone–butanol fermentation, genetic manipulation of the butanol‐producing Clostridium beijerinckii NCIMB 8052, as well as upstream and downstream processing. Specific reference is made to the development of the hyperamylolytic, hyper‐“butanolagenic” C. beijerinckii BA101 strain. The principal problem associated with acetone–butanol fermentation by C. beijerinckii or C. acetobutylicum is butanol toxicity/inhibition to the culture. To solve this problem, we have attempted various alternative in situ/online techniques of butanol removal including membrane‐based systems such as pervaporation, liquid–liquid extraction, and gas stripping.</description><identifier>ISSN: 1527-8999</identifier><identifier>EISSN: 1528-0691</identifier><identifier>DOI: 10.1002/tcr.20023</identifier><identifier>PMID: 15543610</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Acetone - metabolism ; batch ; butanol ; Butanols - analysis ; Butanols - metabolism ; C. beijerinckii BA101 ; Chemical Industry - methods ; Clostridium acetobutylicum - metabolism ; Clostridium beijerinckii ; Clostridium beijerinckii - metabolism ; continuous fermentation ; distillation ; Ethanol - metabolism ; fed-batch ; Fermentation ; gas stripping ; liquid-liquid extraction ; Membranes, Artificial ; pervaporation ; Volatilization</subject><ispartof>Chemical record, 2004, Vol.4 (5), p.305-314</ispartof><rights>2004 The Japan Chemical Journal Forum and Wiley Periodicals, Inc.</rights><rights>(c) 2004 The Japan Chemical Journal Forum and Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4583-f82ac387aea8b91a641da33aaba9b09df5b94a0eac0c271e8c21b42b6fef1f573</citedby><cites>FETCH-LOGICAL-c4583-f82ac387aea8b91a641da33aaba9b09df5b94a0eac0c271e8c21b42b6fef1f573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Ftcr.20023$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Ftcr.20023$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15543610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ezeji, Thaddeus C.</creatorcontrib><creatorcontrib>Qureshi, Nasib</creatorcontrib><creatorcontrib>Blaschek, Hans P.</creatorcontrib><title>Butanol fermentation research: upstream and downstream manipulations</title><title>Chemical record</title><addtitle>Chem Record</addtitle><description>An overview of advances in acetone–butanol fermentation research is presented with specific reference to the history of acetone–butanol fermentation, genetic manipulation of the butanol‐producing Clostridium beijerinckii NCIMB 8052, as well as upstream and downstream processing. Specific reference is made to the development of the hyperamylolytic, hyper‐“butanolagenic” C. beijerinckii BA101 strain. Amylolytic enzyme production by C. beijerinckii BA101 was 1.8‐ and 2.5‐fold greater than that of the C. beijerinckii NCIMB 8052 strain grown in starch and glucose, respectively. We confirmed the presence of a phosphoenolpyruvate (PEP)‐dependent phosphotransferase system (PTS) associated with cell extracts of C. beijerinckii BA101 by glucose phosphorylation by PEP and ATP‐dependent glucose phosphorylation. It was found that C. beijerinckii BA101 was defective in PTS activity and that it compensates for this defect with enhanced glucokinase activity, resulting in an ability to transport and utilize glucose during the solventogenic stage. The principal problem associated with acetone–butanol fermentation by C. beijerinckii or C. acetobutylicum is butanol toxicity/inhibition to the culture. To solve this problem, we have attempted various alternative in situ/online techniques of butanol removal including membrane‐based systems such as pervaporation, liquid–liquid extraction, and gas stripping. We found that gas stripping and pervaporation appear to be the most promising of the in situ acetone–butanol fermentation and recovery techniques but, in terms of cost‐effective industrial applications, gas stripping appears to be the most promising. © 2004 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 4: 305–314; 2004: Published online in Wiley InterScience (www.interscience.wiley.com) DOI 10.1002/tcr.20023
An overview of advances in acetone–butanol fermentation research is presented with specific reference to the history of acetone–butanol fermentation, genetic manipulation of the butanol‐producing Clostridium beijerinckii NCIMB 8052, as well as upstream and downstream processing. Specific reference is made to the development of the hyperamylolytic, hyper‐“butanolagenic” C. beijerinckii BA101 strain. The principal problem associated with acetone–butanol fermentation by C. beijerinckii or C. acetobutylicum is butanol toxicity/inhibition to the culture. To solve this problem, we have attempted various alternative in situ/online techniques of butanol removal including membrane‐based systems such as pervaporation, liquid–liquid extraction, and gas stripping.</description><subject>Acetone - metabolism</subject><subject>batch</subject><subject>butanol</subject><subject>Butanols - analysis</subject><subject>Butanols - metabolism</subject><subject>C. beijerinckii BA101</subject><subject>Chemical Industry - methods</subject><subject>Clostridium acetobutylicum - metabolism</subject><subject>Clostridium beijerinckii</subject><subject>Clostridium beijerinckii - metabolism</subject><subject>continuous fermentation</subject><subject>distillation</subject><subject>Ethanol - metabolism</subject><subject>fed-batch</subject><subject>Fermentation</subject><subject>gas stripping</subject><subject>liquid-liquid extraction</subject><subject>Membranes, Artificial</subject><subject>pervaporation</subject><subject>Volatilization</subject><issn>1527-8999</issn><issn>1528-0691</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kLtOxDAQRS0E4l3wAygVEkUWj53EDh0ssCAQSGgRdNbEmYhAHoudCPh7wu4CFdXckc49xWVsD_gIOBdHnXUjMQS5wjYhFjrkSQqr86xCnabpBtvy_oVzgEipdbYBcRzJBPgmOzvtO2zaKijI1dR02JVtEzjyhM4-Hwf9zHeOsA6wyYO8fW-Wb41NOeurOe532FqBlafd5d1mDxfn0_FleHM3uRqf3IQ2irUMCy3QSq2QUGcpYBJBjlIiZphmPM2LOEsj5ISWW6GAtBWQRSJLCiqgiJXcZgcL78y1bz35ztSlt1RV2FDbeyO4BhFHMICHC9C61ntHhZm5skb3aYCb78nMMJmZTzaw-0tpn9WU_5HLjQbgaAG8lxV9_m8y0_H9jzJcNErf0cdvA92rSZRUsXm8nRj9qMS1uH4yIL8A13qGFg</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Ezeji, Thaddeus C.</creator><creator>Qureshi, Nasib</creator><creator>Blaschek, Hans P.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>2004</creationdate><title>Butanol fermentation research: upstream and downstream manipulations</title><author>Ezeji, Thaddeus C. ; Qureshi, Nasib ; Blaschek, Hans P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4583-f82ac387aea8b91a641da33aaba9b09df5b94a0eac0c271e8c21b42b6fef1f573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Acetone - metabolism</topic><topic>batch</topic><topic>butanol</topic><topic>Butanols - analysis</topic><topic>Butanols - metabolism</topic><topic>C. beijerinckii BA101</topic><topic>Chemical Industry - methods</topic><topic>Clostridium acetobutylicum - metabolism</topic><topic>Clostridium beijerinckii</topic><topic>Clostridium beijerinckii - metabolism</topic><topic>continuous fermentation</topic><topic>distillation</topic><topic>Ethanol - metabolism</topic><topic>fed-batch</topic><topic>Fermentation</topic><topic>gas stripping</topic><topic>liquid-liquid extraction</topic><topic>Membranes, Artificial</topic><topic>pervaporation</topic><topic>Volatilization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ezeji, Thaddeus C.</creatorcontrib><creatorcontrib>Qureshi, Nasib</creatorcontrib><creatorcontrib>Blaschek, Hans P.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Chemical record</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ezeji, Thaddeus C.</au><au>Qureshi, Nasib</au><au>Blaschek, Hans P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Butanol fermentation research: upstream and downstream manipulations</atitle><jtitle>Chemical record</jtitle><addtitle>Chem Record</addtitle><date>2004</date><risdate>2004</risdate><volume>4</volume><issue>5</issue><spage>305</spage><epage>314</epage><pages>305-314</pages><issn>1527-8999</issn><eissn>1528-0691</eissn><abstract>An overview of advances in acetone–butanol fermentation research is presented with specific reference to the history of acetone–butanol fermentation, genetic manipulation of the butanol‐producing Clostridium beijerinckii NCIMB 8052, as well as upstream and downstream processing. Specific reference is made to the development of the hyperamylolytic, hyper‐“butanolagenic” C. beijerinckii BA101 strain. Amylolytic enzyme production by C. beijerinckii BA101 was 1.8‐ and 2.5‐fold greater than that of the C. beijerinckii NCIMB 8052 strain grown in starch and glucose, respectively. We confirmed the presence of a phosphoenolpyruvate (PEP)‐dependent phosphotransferase system (PTS) associated with cell extracts of C. beijerinckii BA101 by glucose phosphorylation by PEP and ATP‐dependent glucose phosphorylation. It was found that C. beijerinckii BA101 was defective in PTS activity and that it compensates for this defect with enhanced glucokinase activity, resulting in an ability to transport and utilize glucose during the solventogenic stage. The principal problem associated with acetone–butanol fermentation by C. beijerinckii or C. acetobutylicum is butanol toxicity/inhibition to the culture. To solve this problem, we have attempted various alternative in situ/online techniques of butanol removal including membrane‐based systems such as pervaporation, liquid–liquid extraction, and gas stripping. We found that gas stripping and pervaporation appear to be the most promising of the in situ acetone–butanol fermentation and recovery techniques but, in terms of cost‐effective industrial applications, gas stripping appears to be the most promising. © 2004 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 4: 305–314; 2004: Published online in Wiley InterScience (www.interscience.wiley.com) DOI 10.1002/tcr.20023
An overview of advances in acetone–butanol fermentation research is presented with specific reference to the history of acetone–butanol fermentation, genetic manipulation of the butanol‐producing Clostridium beijerinckii NCIMB 8052, as well as upstream and downstream processing. Specific reference is made to the development of the hyperamylolytic, hyper‐“butanolagenic” C. beijerinckii BA101 strain. The principal problem associated with acetone–butanol fermentation by C. beijerinckii or C. acetobutylicum is butanol toxicity/inhibition to the culture. To solve this problem, we have attempted various alternative in situ/online techniques of butanol removal including membrane‐based systems such as pervaporation, liquid–liquid extraction, and gas stripping.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15543610</pmid><doi>10.1002/tcr.20023</doi><tpages>10</tpages></addata></record> |
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| subjects | Acetone - metabolism batch butanol Butanols - analysis Butanols - metabolism C. beijerinckii BA101 Chemical Industry - methods Clostridium acetobutylicum - metabolism Clostridium beijerinckii Clostridium beijerinckii - metabolism continuous fermentation distillation Ethanol - metabolism fed-batch Fermentation gas stripping liquid-liquid extraction Membranes, Artificial pervaporation Volatilization |
| title | Butanol fermentation research: upstream and downstream manipulations |
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