Downstream processing of a plant-derived malaria transmission-blocking vaccine candidate

Plants as a platform for recombinant protein expression are now economically comparable to well-established systems, such as microbes and mammalian cells, thanks to advantages such as scalability and product safety. However, downstream processing accounts for the majority of the final product costs...

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Veröffentlicht in:	Protein expression and purification 2018-12, Vol.152, p.122-130
Hauptverfasser:	Menzel, Stephan, Holland, Tanja, Boes, Alexander, Spiegel, Holger, Fischer, Rainer, Buyel, Johannes Felix
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - metabolism Antibodies, Protozoan - chemistry Antibodies, Protozoan - metabolism Blanching Chromatography, Affinity - methods Cloning, Molecular Design of experiments Downstream processing Factor Analysis, Statistical Gene Expression Genetic Vectors - chemistry Genetic Vectors - metabolism Hot Temperature Liquid handling station Malaria Vaccines - biosynthesis Malaria Vaccines - genetics Malaria Vaccines - isolation & purification Nicotiana - chemistry Nicotiana - genetics Nicotiana - metabolism Plant Leaves - genetics Plant Leaves - metabolism Plant Proteins - chemistry Plant Proteins - isolation & purification Plant-derived biopharmaceuticals Plants, Genetically Modified Protein Binding Protein Denaturation Protozoan Proteins - biosynthesis Protozoan Proteins - genetics Protozoan Proteins - isolation & purification Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Sepharose - analogs & derivatives Ultrafiltration - methods
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