Impairment of spermatogenesis and enhancement of testicular germ cell apoptosis induced by exogenous all-Trans-retinoic acid in adult lizard Podarcis sicula

In mammals, retinoic acid is involved in the regulation of testicular function by interaction with two families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR). Among RAR isoforms, the testicular cells of the lizard were found to express only RARα (3.7 kb) and RARβ (...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental zoology. Part A, Comparative experimental biology Comparative experimental biology, 2006-03, Vol.305A (3), p.288-298
Hauptverfasser: Comitato, Raffaella, Esposito, Teresa, Cerbo, Giovanna, Angelini, Francesco, Varriale, Bruno, Cardone, Anna
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 298
container_issue 3
container_start_page 288
container_title Journal of experimental zoology. Part A, Comparative experimental biology
container_volume 305A
creator Comitato, Raffaella
Esposito, Teresa
Cerbo, Giovanna
Angelini, Francesco
Varriale, Bruno
Cardone, Anna
description In mammals, retinoic acid is involved in the regulation of testicular function by interaction with two families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR). Among RAR isoforms, the testicular cells of the lizard were found to express only RARα (3.7 kb) and RARβ (3.4 kb) mRNAs, as reported here. In this study, the effects of exogenous all‐trans‐retinoic acid (atRA) on spermatogenesis of a non‐mammalian seasonal reproducer were investigated. Daily intraperitoneal injections of atRA or atRA plus testosterone (atRA+T) were given for 2 weeks to adult males of the lizard Podarcis sicula. In animals treated with atRA, the seminiferous tubules were markedly reduced in cross‐area. The seminiferous epithelium collapse was responsible for a sensible reduction in the number of germ cells and disruption in normal epithelial organization. In comparison, in atRA+T‐treated lizards the loss of germinal cells was significantly less. The loss of germ cells observed in both experimental groups results from an induction of apoptotic process, as revealed by TUNEL analysis. Although low in number, apoptotic germ cells were also observed in the control groups (saline‐ and T‐treated lizard), where the main germ cells undergoing apoptosis are primary spermatocytes (most frequently) and some spermatogonia. In conclusion, it is shown here that retinoic acid has deleterious effects on lizard spermatogenesis, causing a severe depletion of seminiferous epithelium, probably via induction of apoptotic processes. These effects are not completely inhibited by simultaneous administration of testosterone, although this hormone, once injected, is able to stimulate spermatogenesis and protect germinal cells from apoptotic cell death. J. Exp. Zool. 305A:288–298, 2006. © 2006 Wiley‐Liss, Inc.
doi_str_mv 10.1002/jez.a.264
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_20803268</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20803268</sourcerecordid><originalsourceid>FETCH-LOGICAL-i3164-d28e9a40933a1892041709c5c0b640057403c3d5925f0b5b855255f787a011ff3</originalsourceid><addsrcrecordid>eNpFkctu1jAQhS0EohdY8ALIK3b5seNL7CUqvaEKkGgLYmNNbKe4JE6wE9G_z8LD4tCWrsbSfD4zZw5CryjZUELqt9f-dgObWvInaJcKUVdc629P1zdXldJS76C9nK8LKongz9EOlZzVStNd9Od0mCCkwccZjx3Ok08DzOOVjz6HjCE67OMPiNY_ILPPc7BLDwlfFRhb3_cYpnGax_VHiG6x3uF2i_3NqjMuRabvq_MEMVfJzyGOwWKwwRUYg1v6GffhFpLDn0cHyRaV_G_CC_Ssgz77l_d1H10cHZ4fnFRnn45PD96dVYEVJ5WrldfAiWYMqNI14bQh2gpLWskJEQ0nzDIndC060opWlRMJ0TWqAUJp17F99OZOd0rjr6X4M0PIqy-IvqxvaqIIq6Uq4Ot7cGkH78yUwgBpax7uWYDqDvgder997BOzBmVKUAZMCcp8OPxeyiMf8uxv_vOQfhrZsEaYrx-PjT56f0m_KGkk-wuU_ZbN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20803268</pqid></control><display><type>article</type><title>Impairment of spermatogenesis and enhancement of testicular germ cell apoptosis induced by exogenous all-Trans-retinoic acid in adult lizard Podarcis sicula</title><source>Wiley-Blackwell Journals</source><source>MEDLINE</source><creator>Comitato, Raffaella ; Esposito, Teresa ; Cerbo, Giovanna ; Angelini, Francesco ; Varriale, Bruno ; Cardone, Anna</creator><creatorcontrib>Comitato, Raffaella ; Esposito, Teresa ; Cerbo, Giovanna ; Angelini, Francesco ; Varriale, Bruno ; Cardone, Anna</creatorcontrib><description>In mammals, retinoic acid is involved in the regulation of testicular function by interaction with two families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR). Among RAR isoforms, the testicular cells of the lizard were found to express only RARα (3.7 kb) and RARβ (3.4 kb) mRNAs, as reported here. In this study, the effects of exogenous all‐trans‐retinoic acid (atRA) on spermatogenesis of a non‐mammalian seasonal reproducer were investigated. Daily intraperitoneal injections of atRA or atRA plus testosterone (atRA+T) were given for 2 weeks to adult males of the lizard Podarcis sicula. In animals treated with atRA, the seminiferous tubules were markedly reduced in cross‐area. The seminiferous epithelium collapse was responsible for a sensible reduction in the number of germ cells and disruption in normal epithelial organization. In comparison, in atRA+T‐treated lizards the loss of germinal cells was significantly less. The loss of germ cells observed in both experimental groups results from an induction of apoptotic process, as revealed by TUNEL analysis. Although low in number, apoptotic germ cells were also observed in the control groups (saline‐ and T‐treated lizard), where the main germ cells undergoing apoptosis are primary spermatocytes (most frequently) and some spermatogonia. In conclusion, it is shown here that retinoic acid has deleterious effects on lizard spermatogenesis, causing a severe depletion of seminiferous epithelium, probably via induction of apoptotic processes. These effects are not completely inhibited by simultaneous administration of testosterone, although this hormone, once injected, is able to stimulate spermatogenesis and protect germinal cells from apoptotic cell death. J. Exp. Zool. 305A:288–298, 2006. © 2006 Wiley‐Liss, Inc.</description><identifier>ISSN: 1548-8969</identifier><identifier>ISSN: 1932-5223</identifier><identifier>EISSN: 1552-499X</identifier><identifier>EISSN: 1932-5231</identifier><identifier>DOI: 10.1002/jez.a.264</identifier><identifier>PMID: 16432891</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Apoptosis - drug effects ; Apoptosis - physiology ; Blotting, Northern ; Cell Proliferation - drug effects ; Germ Cells - cytology ; Germ Cells - drug effects ; Germ Cells - metabolism ; Germ Cells - physiology ; Histocytochemistry ; In Situ Nick-End Labeling ; Lacertilia ; Lizards - metabolism ; Lizards - physiology ; Male ; Organ Size - drug effects ; Podarcis sicula ; Random Allocation ; Receptors, Retinoic Acid - genetics ; Receptors, Retinoic Acid - physiology ; RNA - chemistry ; RNA - genetics ; Seminiferous Epithelium - cytology ; Seminiferous Epithelium - drug effects ; Seminiferous Epithelium - metabolism ; Seminiferous Epithelium - physiology ; Spermatogenesis - drug effects ; Testosterone - pharmacology ; Tretinoin - toxicity</subject><ispartof>Journal of experimental zoology. Part A, Comparative experimental biology, 2006-03, Vol.305A (3), p.288-298</ispartof><rights>Copyright © 2006 Wiley‐Liss, Inc., A Wiley Company</rights><rights>Copyright 2006 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjez.a.264$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjez.a.264$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16432891$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Comitato, Raffaella</creatorcontrib><creatorcontrib>Esposito, Teresa</creatorcontrib><creatorcontrib>Cerbo, Giovanna</creatorcontrib><creatorcontrib>Angelini, Francesco</creatorcontrib><creatorcontrib>Varriale, Bruno</creatorcontrib><creatorcontrib>Cardone, Anna</creatorcontrib><title>Impairment of spermatogenesis and enhancement of testicular germ cell apoptosis induced by exogenous all-Trans-retinoic acid in adult lizard Podarcis sicula</title><title>Journal of experimental zoology. Part A, Comparative experimental biology</title><addtitle>J. Exp. Zool</addtitle><description>In mammals, retinoic acid is involved in the regulation of testicular function by interaction with two families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR). Among RAR isoforms, the testicular cells of the lizard were found to express only RARα (3.7 kb) and RARβ (3.4 kb) mRNAs, as reported here. In this study, the effects of exogenous all‐trans‐retinoic acid (atRA) on spermatogenesis of a non‐mammalian seasonal reproducer were investigated. Daily intraperitoneal injections of atRA or atRA plus testosterone (atRA+T) were given for 2 weeks to adult males of the lizard Podarcis sicula. In animals treated with atRA, the seminiferous tubules were markedly reduced in cross‐area. The seminiferous epithelium collapse was responsible for a sensible reduction in the number of germ cells and disruption in normal epithelial organization. In comparison, in atRA+T‐treated lizards the loss of germinal cells was significantly less. The loss of germ cells observed in both experimental groups results from an induction of apoptotic process, as revealed by TUNEL analysis. Although low in number, apoptotic germ cells were also observed in the control groups (saline‐ and T‐treated lizard), where the main germ cells undergoing apoptosis are primary spermatocytes (most frequently) and some spermatogonia. In conclusion, it is shown here that retinoic acid has deleterious effects on lizard spermatogenesis, causing a severe depletion of seminiferous epithelium, probably via induction of apoptotic processes. These effects are not completely inhibited by simultaneous administration of testosterone, although this hormone, once injected, is able to stimulate spermatogenesis and protect germinal cells from apoptotic cell death. J. Exp. Zool. 305A:288–298, 2006. © 2006 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - physiology</subject><subject>Blotting, Northern</subject><subject>Cell Proliferation - drug effects</subject><subject>Germ Cells - cytology</subject><subject>Germ Cells - drug effects</subject><subject>Germ Cells - metabolism</subject><subject>Germ Cells - physiology</subject><subject>Histocytochemistry</subject><subject>In Situ Nick-End Labeling</subject><subject>Lacertilia</subject><subject>Lizards - metabolism</subject><subject>Lizards - physiology</subject><subject>Male</subject><subject>Organ Size - drug effects</subject><subject>Podarcis sicula</subject><subject>Random Allocation</subject><subject>Receptors, Retinoic Acid - genetics</subject><subject>Receptors, Retinoic Acid - physiology</subject><subject>RNA - chemistry</subject><subject>RNA - genetics</subject><subject>Seminiferous Epithelium - cytology</subject><subject>Seminiferous Epithelium - drug effects</subject><subject>Seminiferous Epithelium - metabolism</subject><subject>Seminiferous Epithelium - physiology</subject><subject>Spermatogenesis - drug effects</subject><subject>Testosterone - pharmacology</subject><subject>Tretinoin - toxicity</subject><issn>1548-8969</issn><issn>1932-5223</issn><issn>1552-499X</issn><issn>1932-5231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkctu1jAQhS0EohdY8ALIK3b5seNL7CUqvaEKkGgLYmNNbKe4JE6wE9G_z8LD4tCWrsbSfD4zZw5CryjZUELqt9f-dgObWvInaJcKUVdc629P1zdXldJS76C9nK8LKongz9EOlZzVStNd9Od0mCCkwccZjx3Ok08DzOOVjz6HjCE67OMPiNY_ILPPc7BLDwlfFRhb3_cYpnGax_VHiG6x3uF2i_3NqjMuRabvq_MEMVfJzyGOwWKwwRUYg1v6GffhFpLDn0cHyRaV_G_CC_Ssgz77l_d1H10cHZ4fnFRnn45PD96dVYEVJ5WrldfAiWYMqNI14bQh2gpLWskJEQ0nzDIndC060opWlRMJ0TWqAUJp17F99OZOd0rjr6X4M0PIqy-IvqxvaqIIq6Uq4Ot7cGkH78yUwgBpax7uWYDqDvgder997BOzBmVKUAZMCcp8OPxeyiMf8uxv_vOQfhrZsEaYrx-PjT56f0m_KGkk-wuU_ZbN</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Comitato, Raffaella</creator><creator>Esposito, Teresa</creator><creator>Cerbo, Giovanna</creator><creator>Angelini, Francesco</creator><creator>Varriale, Bruno</creator><creator>Cardone, Anna</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7SN</scope><scope>C1K</scope></search><sort><creationdate>20060301</creationdate><title>Impairment of spermatogenesis and enhancement of testicular germ cell apoptosis induced by exogenous all-Trans-retinoic acid in adult lizard Podarcis sicula</title><author>Comitato, Raffaella ; Esposito, Teresa ; Cerbo, Giovanna ; Angelini, Francesco ; Varriale, Bruno ; Cardone, Anna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3164-d28e9a40933a1892041709c5c0b640057403c3d5925f0b5b855255f787a011ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - physiology</topic><topic>Blotting, Northern</topic><topic>Cell Proliferation - drug effects</topic><topic>Germ Cells - cytology</topic><topic>Germ Cells - drug effects</topic><topic>Germ Cells - metabolism</topic><topic>Germ Cells - physiology</topic><topic>Histocytochemistry</topic><topic>In Situ Nick-End Labeling</topic><topic>Lacertilia</topic><topic>Lizards - metabolism</topic><topic>Lizards - physiology</topic><topic>Male</topic><topic>Organ Size - drug effects</topic><topic>Podarcis sicula</topic><topic>Random Allocation</topic><topic>Receptors, Retinoic Acid - genetics</topic><topic>Receptors, Retinoic Acid - physiology</topic><topic>RNA - chemistry</topic><topic>RNA - genetics</topic><topic>Seminiferous Epithelium - cytology</topic><topic>Seminiferous Epithelium - drug effects</topic><topic>Seminiferous Epithelium - metabolism</topic><topic>Seminiferous Epithelium - physiology</topic><topic>Spermatogenesis - drug effects</topic><topic>Testosterone - pharmacology</topic><topic>Tretinoin - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Comitato, Raffaella</creatorcontrib><creatorcontrib>Esposito, Teresa</creatorcontrib><creatorcontrib>Cerbo, Giovanna</creatorcontrib><creatorcontrib>Angelini, Francesco</creatorcontrib><creatorcontrib>Varriale, Bruno</creatorcontrib><creatorcontrib>Cardone, Anna</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Ecology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of experimental zoology. Part A, Comparative experimental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Comitato, Raffaella</au><au>Esposito, Teresa</au><au>Cerbo, Giovanna</au><au>Angelini, Francesco</au><au>Varriale, Bruno</au><au>Cardone, Anna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impairment of spermatogenesis and enhancement of testicular germ cell apoptosis induced by exogenous all-Trans-retinoic acid in adult lizard Podarcis sicula</atitle><jtitle>Journal of experimental zoology. Part A, Comparative experimental biology</jtitle><addtitle>J. Exp. Zool</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>305A</volume><issue>3</issue><spage>288</spage><epage>298</epage><pages>288-298</pages><issn>1548-8969</issn><issn>1932-5223</issn><eissn>1552-499X</eissn><eissn>1932-5231</eissn><abstract>In mammals, retinoic acid is involved in the regulation of testicular function by interaction with two families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR). Among RAR isoforms, the testicular cells of the lizard were found to express only RARα (3.7 kb) and RARβ (3.4 kb) mRNAs, as reported here. In this study, the effects of exogenous all‐trans‐retinoic acid (atRA) on spermatogenesis of a non‐mammalian seasonal reproducer were investigated. Daily intraperitoneal injections of atRA or atRA plus testosterone (atRA+T) were given for 2 weeks to adult males of the lizard Podarcis sicula. In animals treated with atRA, the seminiferous tubules were markedly reduced in cross‐area. The seminiferous epithelium collapse was responsible for a sensible reduction in the number of germ cells and disruption in normal epithelial organization. In comparison, in atRA+T‐treated lizards the loss of germinal cells was significantly less. The loss of germ cells observed in both experimental groups results from an induction of apoptotic process, as revealed by TUNEL analysis. Although low in number, apoptotic germ cells were also observed in the control groups (saline‐ and T‐treated lizard), where the main germ cells undergoing apoptosis are primary spermatocytes (most frequently) and some spermatogonia. In conclusion, it is shown here that retinoic acid has deleterious effects on lizard spermatogenesis, causing a severe depletion of seminiferous epithelium, probably via induction of apoptotic processes. These effects are not completely inhibited by simultaneous administration of testosterone, although this hormone, once injected, is able to stimulate spermatogenesis and protect germinal cells from apoptotic cell death. J. Exp. Zool. 305A:288–298, 2006. © 2006 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16432891</pmid><doi>10.1002/jez.a.264</doi><tpages>11</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1548-8969
ispartof Journal of experimental zoology. Part A, Comparative experimental biology, 2006-03, Vol.305A (3), p.288-298
issn 1548-8969
1932-5223
1552-499X
1932-5231
language eng
recordid cdi_proquest_miscellaneous_20803268
source Wiley-Blackwell Journals; MEDLINE
subjects Animals
Apoptosis - drug effects
Apoptosis - physiology
Blotting, Northern
Cell Proliferation - drug effects
Germ Cells - cytology
Germ Cells - drug effects
Germ Cells - metabolism
Germ Cells - physiology
Histocytochemistry
In Situ Nick-End Labeling
Lacertilia
Lizards - metabolism
Lizards - physiology
Male
Organ Size - drug effects
Podarcis sicula
Random Allocation
Receptors, Retinoic Acid - genetics
Receptors, Retinoic Acid - physiology
RNA - chemistry
RNA - genetics
Seminiferous Epithelium - cytology
Seminiferous Epithelium - drug effects
Seminiferous Epithelium - metabolism
Seminiferous Epithelium - physiology
Spermatogenesis - drug effects
Testosterone - pharmacology
Tretinoin - toxicity
title Impairment of spermatogenesis and enhancement of testicular germ cell apoptosis induced by exogenous all-Trans-retinoic acid in adult lizard Podarcis sicula
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T17%3A28%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Impairment%20of%20spermatogenesis%20and%20enhancement%20of%20testicular%20germ%20cell%20apoptosis%20induced%20by%20exogenous%20all-Trans-retinoic%20acid%20in%20adult%20lizard%20Podarcis%20sicula&rft.jtitle=Journal%20of%20experimental%20zoology.%20Part%20A,%20Comparative%20experimental%20biology&rft.au=Comitato,%20Raffaella&rft.date=2006-03-01&rft.volume=305A&rft.issue=3&rft.spage=288&rft.epage=298&rft.pages=288-298&rft.issn=1548-8969&rft.eissn=1552-499X&rft_id=info:doi/10.1002/jez.a.264&rft_dat=%3Cproquest_pubme%3E20803268%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20803268&rft_id=info:pmid/16432891&rfr_iscdi=true