Retention Order Reversal of Phosphorylated and Unphosphorylated Peptides in Reversed-Phase LC/MS

Protein phosphorylation is one of the most ubiquitous post-translational modifications in humans, and trypsin-digested phosphorylated peptides have been analyzed by reversed phase LC/MS using C18-silica columns under acidic conditions to profile human phosphoproteomes. Here, we report that phosphope...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical Sciences 2018/09/10, Vol.34(9), pp.1037-1041
Hauptverfasser:	OGATA, Kosuke, KROKHIN, Oleg V., ISHIHAMA, Yasushi
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetic acid Analytical Chemistry Chemistry Formic acid Hydrophobicity ion-pairing linear solvation strength theory Peptides Phosphopeptides Phosphoproteomes Phosphorylation Post-translation Reagents Retention retention order reversal reversed-phase LC Silica Silicon dioxide Solvation Trifluoroacetic acid Trypsin
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!