High-throughput screening method for the identification of active and enantioselective ω-transaminases

A new spectrophotometric determination method using CuSO 4/MeOH was developed to determine the activity of ω-transaminase. Staining solution of CuSO 4/MeOH forms a blue complex with the α-amino acid produced during ω-transaminase reaction. This complex can be easily quantified using UV-Vis spectroph...

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Veröffentlicht in:	Enzyme and microbial technology 2004-04, Vol.34 (5), p.429-436
Hauptverfasser:	Hwang, Bum-Yeol, Kim, Byung-Gee
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Enantioselectivity Enzyme catalysis Enzyme engineering Fundamental and applied biological sciences. Psychology High-throughput screening Methods. Procedures. Technologies Miscellaneous Transferase UV-Vis spectroscopy
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