Staphylococcal cassette chromosome mec (SCCmec) and characterization of the attachment site (attB) of methicillin resistant Staphylococcus aureus (MRSA) and methicillin susceptible Staphylococcus aureus (MSSA) isolates
This study was designed to screen for SCCmec types and to characterize the attachment site (attB) and universal insertion site (orfX) of SCCmec in a collection of 27 isolates (n = 11) methicillin resistant S. aureus and (n = 16) methicillin susceptible S. aureus isolates in Malaysia. Screening of SC...
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| Veröffentlicht in: | Microbial pathogenesis 2018-10, Vol.123, p.323-329 |
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| creator | Bitrus, Asinamai Athliamai Zunita, Zakaria Khairani-Bejo, Siti Othman, Sarah Ahmad Nadzir, Nur Adilah |
| description | This study was designed to screen for SCCmec types and to characterize the attachment site (attB) and universal insertion site (orfX) of SCCmec in a collection of 27 isolates (n = 11) methicillin resistant S. aureus and (n = 16) methicillin susceptible S. aureus isolates in Malaysia. Screening of SCCmec types and characterization of the attachment site was carried out using PCR amplification and Sanger's sequencing method. The result showed that a large proportion of the MRSA isolates carried SCCmec type III 7/11 (63%). Three isolates 3/11 (27%) and 1/11 (9.0%) carried SCCmec type II and IVd respectively. Amplification of the universal insertion site of the SCCmec (orfX) and attachment site (attB) showed that all 16 S. aureus isolates were positive for the orfX gene, while only 7 were positive for the attB gene. Phylogenetic diversity showed that the isolates clustered around strains with features similar to a community acquired MRSA. In conclusion, a high carriage rate of SCCmec type III was observed. The result also showed that all the S. aureus isolates have the orfX structure; however, not all isolates possesses the attB site on the 3′ end of the orfX region.
•High carriage of SCCmec type III (7/11) among MRSA isolates.•Presence of attB attachment site in (7/16) MSSA isolates.•High genetic diversity among isolates. |
| doi_str_mv | 10.1016/j.micpath.2018.07.033 |
| format | Article |
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•High carriage of SCCmec type III (7/11) among MRSA isolates.•Presence of attB attachment site in (7/16) MSSA isolates.•High genetic diversity among isolates.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2018.07.033</identifier><identifier>PMID: 30053600</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Anti-Bacterial Agents - pharmacology ; Attachment Sites, Microbiological - drug effects ; Attachment Sites, Microbiological - genetics ; attB ; Bacterial Proteins - genetics ; Base Sequence ; DNA, Bacterial - genetics ; Genetic Variation - genetics ; Humans ; Malaysia ; Methicillin - pharmacology ; Methicillin-Resistant Staphylococcus aureus - classification ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; Microbial Sensitivity Tests ; Molecular Typing - methods ; MRSA ; MSSA ; ORFX ; Penicillin-Binding Proteins - genetics ; Phylogeny ; Polymerase Chain Reaction - methods ; SCCmec ; Sequence Analysis, DNA ; Staphylococcal Infections - diagnosis ; Staphylococcal Infections - microbiology ; Staphylococcus aureus - classification ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification ; Transcription Factors - genetics</subject><ispartof>Microbial pathogenesis, 2018-10, Vol.123, p.323-329</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-2de487ba0a51e1eae0ca8081f248287c68388b1350ebc7c7d02068fb1ca27b503</citedby><cites>FETCH-LOGICAL-c365t-2de487ba0a51e1eae0ca8081f248287c68388b1350ebc7c7d02068fb1ca27b503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2018.07.033$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30053600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bitrus, Asinamai Athliamai</creatorcontrib><creatorcontrib>Zunita, Zakaria</creatorcontrib><creatorcontrib>Khairani-Bejo, Siti</creatorcontrib><creatorcontrib>Othman, Sarah</creatorcontrib><creatorcontrib>Ahmad Nadzir, Nur Adilah</creatorcontrib><title>Staphylococcal cassette chromosome mec (SCCmec) and characterization of the attachment site (attB) of methicillin resistant Staphylococcus aureus (MRSA) and methicillin susceptible Staphylococcus aureus (MSSA) isolates</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>This study was designed to screen for SCCmec types and to characterize the attachment site (attB) and universal insertion site (orfX) of SCCmec in a collection of 27 isolates (n = 11) methicillin resistant S. aureus and (n = 16) methicillin susceptible S. aureus isolates in Malaysia. Screening of SCCmec types and characterization of the attachment site was carried out using PCR amplification and Sanger's sequencing method. The result showed that a large proportion of the MRSA isolates carried SCCmec type III 7/11 (63%). Three isolates 3/11 (27%) and 1/11 (9.0%) carried SCCmec type II and IVd respectively. Amplification of the universal insertion site of the SCCmec (orfX) and attachment site (attB) showed that all 16 S. aureus isolates were positive for the orfX gene, while only 7 were positive for the attB gene. Phylogenetic diversity showed that the isolates clustered around strains with features similar to a community acquired MRSA. In conclusion, a high carriage rate of SCCmec type III was observed. The result also showed that all the S. aureus isolates have the orfX structure; however, not all isolates possesses the attB site on the 3′ end of the orfX region.
•High carriage of SCCmec type III (7/11) among MRSA isolates.•Presence of attB attachment site in (7/16) MSSA isolates.•High genetic diversity among isolates.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Attachment Sites, Microbiological - drug effects</subject><subject>Attachment Sites, Microbiological - genetics</subject><subject>attB</subject><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>DNA, Bacterial - genetics</subject><subject>Genetic Variation - genetics</subject><subject>Humans</subject><subject>Malaysia</subject><subject>Methicillin - pharmacology</subject><subject>Methicillin-Resistant Staphylococcus aureus - classification</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - isolation & purification</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Typing - methods</subject><subject>MRSA</subject><subject>MSSA</subject><subject>ORFX</subject><subject>Penicillin-Binding Proteins - genetics</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - methods</subject><subject>SCCmec</subject><subject>Sequence Analysis, DNA</subject><subject>Staphylococcal Infections - diagnosis</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus aureus - classification</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Transcription Factors - genetics</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxSMEotvCRwD5uD0kjPPXOaF2BQWpCImFszWZzCpeJfFiO0jlo_Jp8GoX1Avi9CTPe_PG-iXJKwmZBFm_2WeToQOGIctBqgyaDIriSbKS0NapzEE9TVagVJ6WIOEiufR-DwBtWbTPk4sCoCpqgFXyaxvwMDyMliwRjoLQew6BBQ3OTtbbicXEJNbbzSbqtcC5jzN0SIGd-YnB2FnYnQgDCwwBaZh4DsKbuGMdH26vj9OJw2DIjKOZhWNvfMBoety9eIGL4yjrT1-2N6eixzG_eOJDMN3I_wxuj0Hj7YiB_Yvk2Q5Hzy_PepV8e__u6-ZDev_57uPm5j6loq5CmvdcqqZDwEqyZGQgVKDkLi9VrhqqVaFUJ4sKuKOGmh5yqNWuk4R501VQXCXr096Ds98X9kFPJp46jjizXbzOoVFV05ZtG63VyUrOeu94pw_OTOgetAR9xKr3-oxVH7FqaHTEGnOvzxVLN3H_N_WHYzS8PRk4fvSHYac9GZ6Je-OYgu6t-U_Fb_3wu1o</recordid><startdate>201810</startdate><enddate>201810</enddate><creator>Bitrus, Asinamai Athliamai</creator><creator>Zunita, Zakaria</creator><creator>Khairani-Bejo, Siti</creator><creator>Othman, Sarah</creator><creator>Ahmad Nadzir, Nur Adilah</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201810</creationdate><title>Staphylococcal cassette chromosome mec (SCCmec) and characterization of the attachment site (attB) of methicillin resistant Staphylococcus aureus (MRSA) and methicillin susceptible Staphylococcus aureus (MSSA) isolates</title><author>Bitrus, Asinamai Athliamai ; Zunita, Zakaria ; Khairani-Bejo, Siti ; Othman, Sarah ; Ahmad Nadzir, Nur Adilah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-2de487ba0a51e1eae0ca8081f248287c68388b1350ebc7c7d02068fb1ca27b503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Attachment Sites, Microbiological - drug effects</topic><topic>Attachment Sites, Microbiological - genetics</topic><topic>attB</topic><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>DNA, Bacterial - genetics</topic><topic>Genetic Variation - genetics</topic><topic>Humans</topic><topic>Malaysia</topic><topic>Methicillin - pharmacology</topic><topic>Methicillin-Resistant Staphylococcus aureus - classification</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - isolation & purification</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Typing - methods</topic><topic>MRSA</topic><topic>MSSA</topic><topic>ORFX</topic><topic>Penicillin-Binding Proteins - genetics</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - methods</topic><topic>SCCmec</topic><topic>Sequence Analysis, DNA</topic><topic>Staphylococcal Infections - diagnosis</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus aureus - classification</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Transcription Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bitrus, Asinamai Athliamai</creatorcontrib><creatorcontrib>Zunita, Zakaria</creatorcontrib><creatorcontrib>Khairani-Bejo, Siti</creatorcontrib><creatorcontrib>Othman, Sarah</creatorcontrib><creatorcontrib>Ahmad Nadzir, Nur Adilah</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bitrus, Asinamai Athliamai</au><au>Zunita, Zakaria</au><au>Khairani-Bejo, Siti</au><au>Othman, Sarah</au><au>Ahmad Nadzir, Nur Adilah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Staphylococcal cassette chromosome mec (SCCmec) and characterization of the attachment site (attB) of methicillin resistant Staphylococcus aureus (MRSA) and methicillin susceptible Staphylococcus aureus (MSSA) isolates</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2018-10</date><risdate>2018</risdate><volume>123</volume><spage>323</spage><epage>329</epage><pages>323-329</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>This study was designed to screen for SCCmec types and to characterize the attachment site (attB) and universal insertion site (orfX) of SCCmec in a collection of 27 isolates (n = 11) methicillin resistant S. aureus and (n = 16) methicillin susceptible S. aureus isolates in Malaysia. Screening of SCCmec types and characterization of the attachment site was carried out using PCR amplification and Sanger's sequencing method. The result showed that a large proportion of the MRSA isolates carried SCCmec type III 7/11 (63%). Three isolates 3/11 (27%) and 1/11 (9.0%) carried SCCmec type II and IVd respectively. Amplification of the universal insertion site of the SCCmec (orfX) and attachment site (attB) showed that all 16 S. aureus isolates were positive for the orfX gene, while only 7 were positive for the attB gene. Phylogenetic diversity showed that the isolates clustered around strains with features similar to a community acquired MRSA. In conclusion, a high carriage rate of SCCmec type III was observed. The result also showed that all the S. aureus isolates have the orfX structure; however, not all isolates possesses the attB site on the 3′ end of the orfX region.
•High carriage of SCCmec type III (7/11) among MRSA isolates.•Presence of attB attachment site in (7/16) MSSA isolates.•High genetic diversity among isolates.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>30053600</pmid><doi>10.1016/j.micpath.2018.07.033</doi><tpages>7</tpages></addata></record> |
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| ispartof | Microbial pathogenesis, 2018-10, Vol.123, p.323-329 |
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| subjects | Anti-Bacterial Agents - pharmacology Attachment Sites, Microbiological - drug effects Attachment Sites, Microbiological - genetics attB Bacterial Proteins - genetics Base Sequence DNA, Bacterial - genetics Genetic Variation - genetics Humans Malaysia Methicillin - pharmacology Methicillin-Resistant Staphylococcus aureus - classification Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - genetics Methicillin-Resistant Staphylococcus aureus - isolation & purification Microbial Sensitivity Tests Molecular Typing - methods MRSA MSSA ORFX Penicillin-Binding Proteins - genetics Phylogeny Polymerase Chain Reaction - methods SCCmec Sequence Analysis, DNA Staphylococcal Infections - diagnosis Staphylococcal Infections - microbiology Staphylococcus aureus - classification Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Transcription Factors - genetics |
| title | Staphylococcal cassette chromosome mec (SCCmec) and characterization of the attachment site (attB) of methicillin resistant Staphylococcus aureus (MRSA) and methicillin susceptible Staphylococcus aureus (MSSA) isolates |
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