Error-prone replication of West Nile virus caused by ribavirin
Ribavirin has been reported to cause error-prone replication and viral extinction in RNA viruses. The antiviral activity of ribavirin against West Nile virus (WNV) was evaluated in various cell lines to select a model in which mutagenic effects could be studied. The antiviral activity was greatest i...
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| Veröffentlicht in: | Antiviral research 2005-07, Vol.67 (1), p.38-45 |
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| creator | Day, Craig W. Smee, Donald F. Julander, Justin G. Yamshchikov, Vladimir F. Sidwell, Robert W. Morrey, John D. |
| description | Ribavirin has been reported to cause error-prone replication and viral extinction in RNA viruses. The antiviral activity of ribavirin against West Nile virus (WNV) was evaluated in various cell lines to select a model in which mutagenic effects could be studied. The antiviral activity was greatest in HeLa cells as compared to CV-1, L929, Vero, or MA-104 cells. WNV was also passaged sequentially in cell monolayers treated with ribavirin to determine whether cumulative mutations could lead to viral extinction in these cell lines. The virus was abrogated in HeLa cells after 4 passages, while high viral titers persisted after many passages in other cells. A molecular clone of WNV was propagated in HeLa cells treated with 15
μg/mL ribavirin, and sequencing of viral genome segments revealed significant increases in transition mutations, demonstrating that ribavirin induced error-prone replication. The relative infectivity of viral RNA synthesized in the presence of ribavirin was shown to be reduced compared with untreated controls. These data support the hypothesis that error catastrophe is one of the modes of action for ribavirin against WNV. |
| doi_str_mv | 10.1016/j.antiviral.2005.04.002 |
| format | Article |
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μg/mL ribavirin, and sequencing of viral genome segments revealed significant increases in transition mutations, demonstrating that ribavirin induced error-prone replication. The relative infectivity of viral RNA synthesized in the presence of ribavirin was shown to be reduced compared with untreated controls. These data support the hypothesis that error catastrophe is one of the modes of action for ribavirin against WNV.</description><identifier>ISSN: 0166-3542</identifier><identifier>EISSN: 1872-9096</identifier><identifier>DOI: 10.1016/j.antiviral.2005.04.002</identifier><identifier>PMID: 15919121</identifier><identifier>CODEN: ARSRDR</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antiviral ; Antiviral agents ; Antiviral Agents - pharmacology ; Antiviral Agents - toxicity ; Biological and medical sciences ; Cell Line ; Cercopithecus aethiops ; Error catastrophe ; HeLa Cells ; Humans ; Medical sciences ; Mutagenesis ; Mutation ; Pharmacology. Drug treatments ; Reverse Transcriptase Polymerase Chain Reaction ; Ribavirin ; Ribavirin - pharmacology ; Ribavirin - toxicity ; RNA, Viral - genetics ; Sequence Analysis, DNA ; Vero Cells ; Virus Replication - drug effects ; West Nile virus ; West Nile virus - drug effects ; West Nile virus - genetics ; West Nile virus - physiology</subject><ispartof>Antiviral research, 2005-07, Vol.67 (1), p.38-45</ispartof><rights>2005 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-817d7a0770824c523052bf78d14747b68ae392828308c3341e2d30dbf926d2ad3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.antiviral.2005.04.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16885889$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15919121$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Day, Craig W.</creatorcontrib><creatorcontrib>Smee, Donald F.</creatorcontrib><creatorcontrib>Julander, Justin G.</creatorcontrib><creatorcontrib>Yamshchikov, Vladimir F.</creatorcontrib><creatorcontrib>Sidwell, Robert W.</creatorcontrib><creatorcontrib>Morrey, John D.</creatorcontrib><title>Error-prone replication of West Nile virus caused by ribavirin</title><title>Antiviral research</title><addtitle>Antiviral Res</addtitle><description>Ribavirin has been reported to cause error-prone replication and viral extinction in RNA viruses. The antiviral activity of ribavirin against West Nile virus (WNV) was evaluated in various cell lines to select a model in which mutagenic effects could be studied. The antiviral activity was greatest in HeLa cells as compared to CV-1, L929, Vero, or MA-104 cells. WNV was also passaged sequentially in cell monolayers treated with ribavirin to determine whether cumulative mutations could lead to viral extinction in these cell lines. The virus was abrogated in HeLa cells after 4 passages, while high viral titers persisted after many passages in other cells. A molecular clone of WNV was propagated in HeLa cells treated with 15
μg/mL ribavirin, and sequencing of viral genome segments revealed significant increases in transition mutations, demonstrating that ribavirin induced error-prone replication. The relative infectivity of viral RNA synthesized in the presence of ribavirin was shown to be reduced compared with untreated controls. These data support the hypothesis that error catastrophe is one of the modes of action for ribavirin against WNV.</description><subject>Animals</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antiviral</subject><subject>Antiviral agents</subject><subject>Antiviral Agents - pharmacology</subject><subject>Antiviral Agents - toxicity</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cercopithecus aethiops</subject><subject>Error catastrophe</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mutagenesis</subject><subject>Mutation</subject><subject>Pharmacology. Drug treatments</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Ribavirin</subject><subject>Ribavirin - pharmacology</subject><subject>Ribavirin - toxicity</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Vero Cells</subject><subject>Virus Replication - drug effects</subject><subject>West Nile virus</subject><subject>West Nile virus - drug effects</subject><subject>West Nile virus - genetics</subject><subject>West Nile virus - physiology</subject><issn>0166-3542</issn><issn>1872-9096</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMouq7-Be1Fb62TtE3SiyCyfsCiF8VjSJMpZOm2a9Iu-O_NskWPexoYnnfm5SHkmkJGgfK7Vaa7wW2d123GAMoMigyAHZEZlYKlFVT8mMwiydO8LNgZOQ9hBQBcVPKUnNGyohVldEbuF973Pt34vsPE46Z1Rg-u75K-Sb4wDMmbazGJj8aQGD0GtEn9k3hX67hz3QU5aXQb8HKac_L5tPh4fEmX78-vjw_L1BScDqmkwgoNQoBkhSlZDiWrGyEtLUQhai415hWTTOYgTZ4XFJnNwdZNxbhl2uZzcru_G4t-j7GXWrtgsG11h_0YFIu3ZcnpQZCKEiK3A8UeNL4PwWOjNt6ttf9RFNTOsVqpP8dq51hBoaLjmLyaXoz1Gu1_bpIagZsJ0MHotvG6My78c1zKUsoqcg97DqO5rUOvgnHYGbTOoxmU7d3BMr9ef5zS</recordid><startdate>20050701</startdate><enddate>20050701</enddate><creator>Day, Craig W.</creator><creator>Smee, Donald F.</creator><creator>Julander, Justin G.</creator><creator>Yamshchikov, Vladimir F.</creator><creator>Sidwell, Robert W.</creator><creator>Morrey, John D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20050701</creationdate><title>Error-prone replication of West Nile virus caused by ribavirin</title><author>Day, Craig W. ; Smee, Donald F. ; Julander, Justin G. ; Yamshchikov, Vladimir F. ; Sidwell, Robert W. ; Morrey, John D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-817d7a0770824c523052bf78d14747b68ae392828308c3341e2d30dbf926d2ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antiviral</topic><topic>Antiviral agents</topic><topic>Antiviral Agents - pharmacology</topic><topic>Antiviral Agents - toxicity</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cercopithecus aethiops</topic><topic>Error catastrophe</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mutagenesis</topic><topic>Mutation</topic><topic>Pharmacology. Drug treatments</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Ribavirin</topic><topic>Ribavirin - pharmacology</topic><topic>Ribavirin - toxicity</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Vero Cells</topic><topic>Virus Replication - drug effects</topic><topic>West Nile virus</topic><topic>West Nile virus - drug effects</topic><topic>West Nile virus - genetics</topic><topic>West Nile virus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Day, Craig W.</creatorcontrib><creatorcontrib>Smee, Donald F.</creatorcontrib><creatorcontrib>Julander, Justin G.</creatorcontrib><creatorcontrib>Yamshchikov, Vladimir F.</creatorcontrib><creatorcontrib>Sidwell, Robert W.</creatorcontrib><creatorcontrib>Morrey, John D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Antiviral research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Day, Craig W.</au><au>Smee, Donald F.</au><au>Julander, Justin G.</au><au>Yamshchikov, Vladimir F.</au><au>Sidwell, Robert W.</au><au>Morrey, John D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Error-prone replication of West Nile virus caused by ribavirin</atitle><jtitle>Antiviral research</jtitle><addtitle>Antiviral Res</addtitle><date>2005-07-01</date><risdate>2005</risdate><volume>67</volume><issue>1</issue><spage>38</spage><epage>45</epage><pages>38-45</pages><issn>0166-3542</issn><eissn>1872-9096</eissn><coden>ARSRDR</coden><abstract>Ribavirin has been reported to cause error-prone replication and viral extinction in RNA viruses. The antiviral activity of ribavirin against West Nile virus (WNV) was evaluated in various cell lines to select a model in which mutagenic effects could be studied. The antiviral activity was greatest in HeLa cells as compared to CV-1, L929, Vero, or MA-104 cells. WNV was also passaged sequentially in cell monolayers treated with ribavirin to determine whether cumulative mutations could lead to viral extinction in these cell lines. The virus was abrogated in HeLa cells after 4 passages, while high viral titers persisted after many passages in other cells. A molecular clone of WNV was propagated in HeLa cells treated with 15
μg/mL ribavirin, and sequencing of viral genome segments revealed significant increases in transition mutations, demonstrating that ribavirin induced error-prone replication. The relative infectivity of viral RNA synthesized in the presence of ribavirin was shown to be reduced compared with untreated controls. These data support the hypothesis that error catastrophe is one of the modes of action for ribavirin against WNV.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15919121</pmid><doi>10.1016/j.antiviral.2005.04.002</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antiviral Antiviral agents Antiviral Agents - pharmacology Antiviral Agents - toxicity Biological and medical sciences Cell Line Cercopithecus aethiops Error catastrophe HeLa Cells Humans Medical sciences Mutagenesis Mutation Pharmacology. Drug treatments Reverse Transcriptase Polymerase Chain Reaction Ribavirin Ribavirin - pharmacology Ribavirin - toxicity RNA, Viral - genetics Sequence Analysis, DNA Vero Cells Virus Replication - drug effects West Nile virus West Nile virus - drug effects West Nile virus - genetics West Nile virus - physiology |
| title | Error-prone replication of West Nile virus caused by ribavirin |
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