Environmental surveillance of wild poliovirus circulation in Egypt—Balancing between detection sensitivity and workload

Examination of sewage specimens for poliovirus (environmental surveillance) was adopted as a supplementary tool in the surveillance of poliomyelitis in Egypt. Sewage samples were concentrated about 50-fold using a simple two-phase separation technique, and inoculated in cell cultures in two collabor...

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Veröffentlicht in:	Journal of virological methods 2005-06, Vol.126 (1), p.127-134
Hauptverfasser:	Hovi, Tapani, Blomqvist, Soile, Nasr, Eman, Burns, Cara C., Sarjakoski, Tarja, Ahmed, Nahed, Savolainen, Carita, Roivainen, Merja, Stenvik, Mirja, Laine, Pia, Barakat, Ibrahim, Wahdan, Mohammed H., Kamel, Faten A., Asghar, Humayun, Pallansch, Mark A., Kew, Olen M., Gary, Howard E., deGourville, Esther M., Bassioni, Laila El
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Capsid Proteins - genetics Cell Line Concentration methods Detection methods DNA, Viral - chemistry Egypt Environmental surveillance Fundamental and applied biological sciences. Psychology Humans Mice Microbiology Molecular Sequence Data Phylogeny Poliovirus Poliovirus - isolation & purification Population Surveillance - methods Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - analysis RNA, Viral - genetics Sequence Analysis, DNA Sequence Homology Sewage - virology Techniques used in virology Virology Virus Cultivation Virus mixture
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