Environmental surveillance of wild poliovirus circulation in Egypt—Balancing between detection sensitivity and workload
Examination of sewage specimens for poliovirus (environmental surveillance) was adopted as a supplementary tool in the surveillance of poliomyelitis in Egypt. Sewage samples were concentrated about 50-fold using a simple two-phase separation technique, and inoculated in cell cultures in two collabor...
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| Veröffentlicht in: | Journal of virological methods 2005-06, Vol.126 (1), p.127-134 |
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| creator | Hovi, Tapani Blomqvist, Soile Nasr, Eman Burns, Cara C. Sarjakoski, Tarja Ahmed, Nahed Savolainen, Carita Roivainen, Merja Stenvik, Mirja Laine, Pia Barakat, Ibrahim Wahdan, Mohammed H. Kamel, Faten A. Asghar, Humayun Pallansch, Mark A. Kew, Olen M. Gary, Howard E. deGourville, Esther M. Bassioni, Laila El |
| description | Examination of sewage specimens for poliovirus (environmental surveillance) was adopted as a supplementary tool in the surveillance of poliomyelitis in Egypt. Sewage samples were concentrated about 50-fold using a simple two-phase separation technique, and inoculated in cell cultures in two collaborating laboratories in parallel. All but 9 of the 293 (97%) samples collected from January 2001 to December 2002 contained poliovirus and/or other enteroviruses, with polioviruses being detected in 84% of the samples. The proportion of specimens containing type 1 wild poliovirus (PV1W, the North-East African (NEAF) genotype) was less in 2002 (16%) than in 2001 (57%), and further decreased in 2003. While the overall sensitivity to detect PV1W was similar in the two collaborating laboratories, the specimens scored positive were not identical. Parallel cultures inoculated with aliquots of a given specimen very frequently resulted in isolation of different viruses. Moreover, partial sequence analysis occasionally revealed representatives of different genetic lineages of PV1W in a given specimen. These results emphasize the need to use intensive laboratory analysis to optimise sample sensitivity in environmental poliovirus surveillance, and the difficulties in reproducing the isolation results by simple re-inoculation of samples containing a mixture of different viruses. |
| doi_str_mv | 10.1016/j.jviromet.2005.02.002 |
| format | Article |
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Sewage samples were concentrated about 50-fold using a simple two-phase separation technique, and inoculated in cell cultures in two collaborating laboratories in parallel. All but 9 of the 293 (97%) samples collected from January 2001 to December 2002 contained poliovirus and/or other enteroviruses, with polioviruses being detected in 84% of the samples. The proportion of specimens containing type 1 wild poliovirus (PV1W, the North-East African (NEAF) genotype) was less in 2002 (16%) than in 2001 (57%), and further decreased in 2003. While the overall sensitivity to detect PV1W was similar in the two collaborating laboratories, the specimens scored positive were not identical. Parallel cultures inoculated with aliquots of a given specimen very frequently resulted in isolation of different viruses. Moreover, partial sequence analysis occasionally revealed representatives of different genetic lineages of PV1W in a given specimen. These results emphasize the need to use intensive laboratory analysis to optimise sample sensitivity in environmental poliovirus surveillance, and the difficulties in reproducing the isolation results by simple re-inoculation of samples containing a mixture of different viruses.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2005.02.002</identifier><identifier>PMID: 15847928</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Capsid Proteins - genetics ; Cell Line ; Concentration methods ; Detection methods ; DNA, Viral - chemistry ; Egypt ; Environmental surveillance ; Fundamental and applied biological sciences. Psychology ; Humans ; Mice ; Microbiology ; Molecular Sequence Data ; Phylogeny ; Poliovirus ; Poliovirus - isolation & purification ; Population Surveillance - methods ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - analysis ; RNA, Viral - genetics ; Sequence Analysis, DNA ; Sequence Homology ; Sewage - virology ; Techniques used in virology ; Virology ; Virus Cultivation ; Virus mixture</subject><ispartof>Journal of virological methods, 2005-06, Vol.126 (1), p.127-134</ispartof><rights>2005 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-7e6a2aab9a1efd2135b3a1559aab32b624a518730dcbc8fa07226c6323c595ee3</citedby><cites>FETCH-LOGICAL-c458t-7e6a2aab9a1efd2135b3a1559aab32b624a518730dcbc8fa07226c6323c595ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0166093405000467$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16737648$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15847928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hovi, Tapani</creatorcontrib><creatorcontrib>Blomqvist, Soile</creatorcontrib><creatorcontrib>Nasr, Eman</creatorcontrib><creatorcontrib>Burns, Cara C.</creatorcontrib><creatorcontrib>Sarjakoski, Tarja</creatorcontrib><creatorcontrib>Ahmed, Nahed</creatorcontrib><creatorcontrib>Savolainen, Carita</creatorcontrib><creatorcontrib>Roivainen, Merja</creatorcontrib><creatorcontrib>Stenvik, Mirja</creatorcontrib><creatorcontrib>Laine, Pia</creatorcontrib><creatorcontrib>Barakat, Ibrahim</creatorcontrib><creatorcontrib>Wahdan, Mohammed H.</creatorcontrib><creatorcontrib>Kamel, Faten A.</creatorcontrib><creatorcontrib>Asghar, Humayun</creatorcontrib><creatorcontrib>Pallansch, Mark A.</creatorcontrib><creatorcontrib>Kew, Olen M.</creatorcontrib><creatorcontrib>Gary, Howard E.</creatorcontrib><creatorcontrib>deGourville, Esther M.</creatorcontrib><creatorcontrib>Bassioni, Laila El</creatorcontrib><title>Environmental surveillance of wild poliovirus circulation in Egypt—Balancing between detection sensitivity and workload</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>Examination of sewage specimens for poliovirus (environmental surveillance) was adopted as a supplementary tool in the surveillance of poliomyelitis in Egypt. Sewage samples were concentrated about 50-fold using a simple two-phase separation technique, and inoculated in cell cultures in two collaborating laboratories in parallel. All but 9 of the 293 (97%) samples collected from January 2001 to December 2002 contained poliovirus and/or other enteroviruses, with polioviruses being detected in 84% of the samples. The proportion of specimens containing type 1 wild poliovirus (PV1W, the North-East African (NEAF) genotype) was less in 2002 (16%) than in 2001 (57%), and further decreased in 2003. While the overall sensitivity to detect PV1W was similar in the two collaborating laboratories, the specimens scored positive were not identical. Parallel cultures inoculated with aliquots of a given specimen very frequently resulted in isolation of different viruses. Moreover, partial sequence analysis occasionally revealed representatives of different genetic lineages of PV1W in a given specimen. 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Psychology</subject><subject>Humans</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Poliovirus</subject><subject>Poliovirus - isolation & purification</subject><subject>Population Surveillance - methods</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>Sewage - virology</subject><subject>Techniques used in virology</subject><subject>Virology</subject><subject>Virus Cultivation</subject><subject>Virus mixture</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQhy0EokvhFSpf4LbBf2I7uQHVtiBV6gXOluNMKi-JvdjOrvbGQ_CEfRIcdlGPPdmyvt94Zj6EriipKKHy47ba7l0ME-SKESIqwipC2Au0oo1q16Rt6pdoVUBZ7ry-QG9S2pICKs5fowsqmlq1rFmh48YvdfwEPpsRpznuwY2j8RZwGPDBjT3ehdGFQs0JWxftPJrsgsfO483DcZcff__5YpaE8w-4g3wA8LiHDPYflsAnl93e5SM2vseHEH-OwfRv0avBjAnenc9L9ONm8_366_ru_vbb9ee7ta1Fk9cKpGHGdK2hMPSMctFxQ4VoyxtnnWS1EWVmTnrb2WYwRDEmreSMW9EKAH6JPpzq7mL4NUPKenLJwjIjhDlpRpRSLSfPglQJUjPeFlCeQBtDShEGvYtuMvGoKdGLHb3V_-3oxY4mTBc7JXh1_mHuJuifYmcdBXh_BkyyZhzistX0xEnFlawX7tOJg7K4vYOok3VQnPUulrXrPrjnevkLyVW19w</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Hovi, Tapani</creator><creator>Blomqvist, Soile</creator><creator>Nasr, Eman</creator><creator>Burns, Cara C.</creator><creator>Sarjakoski, Tarja</creator><creator>Ahmed, Nahed</creator><creator>Savolainen, Carita</creator><creator>Roivainen, Merja</creator><creator>Stenvik, Mirja</creator><creator>Laine, Pia</creator><creator>Barakat, Ibrahim</creator><creator>Wahdan, Mohammed H.</creator><creator>Kamel, Faten A.</creator><creator>Asghar, Humayun</creator><creator>Pallansch, Mark A.</creator><creator>Kew, Olen M.</creator><creator>Gary, Howard E.</creator><creator>deGourville, Esther M.</creator><creator>Bassioni, Laila El</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20050601</creationdate><title>Environmental surveillance of wild poliovirus circulation in Egypt—Balancing between detection sensitivity and workload</title><author>Hovi, Tapani ; Blomqvist, Soile ; Nasr, Eman ; Burns, Cara C. ; Sarjakoski, Tarja ; Ahmed, Nahed ; Savolainen, Carita ; Roivainen, Merja ; Stenvik, Mirja ; Laine, Pia ; Barakat, Ibrahim ; Wahdan, Mohammed H. ; Kamel, Faten A. ; Asghar, Humayun ; Pallansch, Mark A. ; Kew, Olen M. ; Gary, Howard E. ; deGourville, Esther M. ; Bassioni, Laila El</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-7e6a2aab9a1efd2135b3a1559aab32b624a518730dcbc8fa07226c6323c595ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Capsid Proteins - genetics</topic><topic>Cell Line</topic><topic>Concentration methods</topic><topic>Detection methods</topic><topic>DNA, Viral - chemistry</topic><topic>Egypt</topic><topic>Environmental surveillance</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Poliovirus</topic><topic>Poliovirus - isolation & purification</topic><topic>Population Surveillance - methods</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - analysis</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology</topic><topic>Sewage - virology</topic><topic>Techniques used in virology</topic><topic>Virology</topic><topic>Virus Cultivation</topic><topic>Virus mixture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hovi, Tapani</creatorcontrib><creatorcontrib>Blomqvist, Soile</creatorcontrib><creatorcontrib>Nasr, Eman</creatorcontrib><creatorcontrib>Burns, Cara C.</creatorcontrib><creatorcontrib>Sarjakoski, Tarja</creatorcontrib><creatorcontrib>Ahmed, Nahed</creatorcontrib><creatorcontrib>Savolainen, Carita</creatorcontrib><creatorcontrib>Roivainen, Merja</creatorcontrib><creatorcontrib>Stenvik, Mirja</creatorcontrib><creatorcontrib>Laine, Pia</creatorcontrib><creatorcontrib>Barakat, Ibrahim</creatorcontrib><creatorcontrib>Wahdan, Mohammed H.</creatorcontrib><creatorcontrib>Kamel, Faten A.</creatorcontrib><creatorcontrib>Asghar, Humayun</creatorcontrib><creatorcontrib>Pallansch, Mark A.</creatorcontrib><creatorcontrib>Kew, Olen M.</creatorcontrib><creatorcontrib>Gary, Howard E.</creatorcontrib><creatorcontrib>deGourville, Esther M.</creatorcontrib><creatorcontrib>Bassioni, Laila El</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hovi, Tapani</au><au>Blomqvist, Soile</au><au>Nasr, Eman</au><au>Burns, Cara C.</au><au>Sarjakoski, Tarja</au><au>Ahmed, Nahed</au><au>Savolainen, Carita</au><au>Roivainen, Merja</au><au>Stenvik, Mirja</au><au>Laine, Pia</au><au>Barakat, Ibrahim</au><au>Wahdan, Mohammed H.</au><au>Kamel, Faten A.</au><au>Asghar, Humayun</au><au>Pallansch, Mark A.</au><au>Kew, Olen M.</au><au>Gary, Howard E.</au><au>deGourville, Esther M.</au><au>Bassioni, Laila El</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Environmental surveillance of wild poliovirus circulation in Egypt—Balancing between detection sensitivity and workload</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>126</volume><issue>1</issue><spage>127</spage><epage>134</epage><pages>127-134</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>Examination of sewage specimens for poliovirus (environmental surveillance) was adopted as a supplementary tool in the surveillance of poliomyelitis in Egypt. Sewage samples were concentrated about 50-fold using a simple two-phase separation technique, and inoculated in cell cultures in two collaborating laboratories in parallel. All but 9 of the 293 (97%) samples collected from January 2001 to December 2002 contained poliovirus and/or other enteroviruses, with polioviruses being detected in 84% of the samples. The proportion of specimens containing type 1 wild poliovirus (PV1W, the North-East African (NEAF) genotype) was less in 2002 (16%) than in 2001 (57%), and further decreased in 2003. While the overall sensitivity to detect PV1W was similar in the two collaborating laboratories, the specimens scored positive were not identical. Parallel cultures inoculated with aliquots of a given specimen very frequently resulted in isolation of different viruses. Moreover, partial sequence analysis occasionally revealed representatives of different genetic lineages of PV1W in a given specimen. 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| subjects | Animals Biological and medical sciences Capsid Proteins - genetics Cell Line Concentration methods Detection methods DNA, Viral - chemistry Egypt Environmental surveillance Fundamental and applied biological sciences. Psychology Humans Mice Microbiology Molecular Sequence Data Phylogeny Poliovirus Poliovirus - isolation & purification Population Surveillance - methods Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - analysis RNA, Viral - genetics Sequence Analysis, DNA Sequence Homology Sewage - virology Techniques used in virology Virology Virus Cultivation Virus mixture |
| title | Environmental surveillance of wild poliovirus circulation in Egypt—Balancing between detection sensitivity and workload |
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