Sequential observation of 2,6-dimethylaniline-induced nasal lesions in a rat two-stage nasal carcinogenesis model after initiation with N-bis(2-hydroxypropyl) nitrosamine
Male F344 rats received diet containing 3,000 ppm 2,6-dimethylaniline (DMA) after initiation with a single subcutaneous injection of 2,400 mg/kg of N-bis(2-hydroxypropyl)nitrosamine (DHPN), and histological and electron microscopic examinations of the nasal cavity were performed at 4, 13, 26 and 52...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2000, Vol.62(7), pp.751-756 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | Koujitani, T. (National Inst. of Health Science, Tokyo (Japan)) Yasuhara, K Ikeda, T Imazawa, T Tamura, T Toyosawa, K Shimada, A Hirose, M Mitsumori, K |
| description | Male F344 rats received diet containing 3,000 ppm 2,6-dimethylaniline (DMA) after initiation with a single subcutaneous injection of 2,400 mg/kg of N-bis(2-hydroxypropyl)nitrosamine (DHPN), and histological and electron microscopic examinations of the nasal cavity were performed at 4, 13, 26 and 52 weeks to examine sequential changes induced by DMA. Severe atrophy of Bowman's glands and epithelial disarrangement were apparent from week 4, followed by dilatation and/or proliferation of Bowman's glands, degeneration of epithelial cells, and proliferation of undifferentiated epithelial cells from week 13. Focal glandular hyperplasias, dysplastic foci, and adenomas were observed from week 26, and carcinomas at 52 week. These nasal lesions were mostly evident in the olfactory mucosa in the nasal cavity, and their severity and/or incidences, other than atrophy of Bowman's glands, increased with the treatment period. Electron microscopically, carcinoma cells demonstrated desmosomes, dense secretory granules identical to those in normal Bowman's glands, a basement membrane, and microvilli. These results suggest that Bowman's glands are the target of DMA, giving rise to nasal carcinomas after DHPN-initiation. |
| doi_str_mv | 10.1292/jvms.62.751 |
| format | Article |
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(National Inst. of Health Science, Tokyo (Japan)) ; Yasuhara, K ; Ikeda, T ; Imazawa, T ; Tamura, T ; Toyosawa, K ; Shimada, A ; Hirose, M ; Mitsumori, K</creator><creatorcontrib>Koujitani, T. (National Inst. of Health Science, Tokyo (Japan)) ; Yasuhara, K ; Ikeda, T ; Imazawa, T ; Tamura, T ; Toyosawa, K ; Shimada, A ; Hirose, M ; Mitsumori, K</creatorcontrib><description>Male F344 rats received diet containing 3,000 ppm 2,6-dimethylaniline (DMA) after initiation with a single subcutaneous injection of 2,400 mg/kg of N-bis(2-hydroxypropyl)nitrosamine (DHPN), and histological and electron microscopic examinations of the nasal cavity were performed at 4, 13, 26 and 52 weeks to examine sequential changes induced by DMA. Severe atrophy of Bowman's glands and epithelial disarrangement were apparent from week 4, followed by dilatation and/or proliferation of Bowman's glands, degeneration of epithelial cells, and proliferation of undifferentiated epithelial cells from week 13. Focal glandular hyperplasias, dysplastic foci, and adenomas were observed from week 26, and carcinomas at 52 week. These nasal lesions were mostly evident in the olfactory mucosa in the nasal cavity, and their severity and/or incidences, other than atrophy of Bowman's glands, increased with the treatment period. Electron microscopically, carcinoma cells demonstrated desmosomes, dense secretory granules identical to those in normal Bowman's glands, a basement membrane, and microvilli. These results suggest that Bowman's glands are the target of DMA, giving rise to nasal carcinomas after DHPN-initiation.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.62.751</identifier><identifier>PMID: 10945294</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>2,6-dimethylaniline ; Adenoma - chemically induced ; Adenoma - pathology ; Aniline Compounds ; Animals ; Atrophy ; CARCINOGENESIS ; carcinogenicity ; Carcinogens ; Carcinoma - chemically induced ; Carcinoma - pathology ; Cell Division ; Cell Nucleus - pathology ; Cell Nucleus - ultrastructure ; HISTOCYTOLOGICAL ANALYSIS ; Hyperplasia ; Male ; MUTAGENS ; N-bis(2-hydroxypropyl)nitrosamine ; nasal cavity ; Nasal Mucosa - cytology ; Nasal Mucosa - drug effects ; Nasal Mucosa - pathology ; NITROSAMINES ; NOSE ; Nose Neoplasms - chemically induced ; Nose Neoplasms - pathology ; Nose Neoplasms - ultrastructure ; Olfactory Mucosa - cytology ; Olfactory Mucosa - drug effects ; Olfactory Mucosa - pathology ; rat ; RATS ; Rats, Inbred F344 ; Time Factors</subject><ispartof>Journal of Veterinary Medical Science, 2000, Vol.62(7), pp.751-756</ispartof><rights>2000 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c551t-b88691ca4dee4feaf0ed5d347a3ec48e7a72d4cf868b37239a0ba7ad5d0fdb793</citedby><cites>FETCH-LOGICAL-c551t-b88691ca4dee4feaf0ed5d347a3ec48e7a72d4cf868b37239a0ba7ad5d0fdb793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10945294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koujitani, T. (National Inst. of Health Science, Tokyo (Japan))</creatorcontrib><creatorcontrib>Yasuhara, K</creatorcontrib><creatorcontrib>Ikeda, T</creatorcontrib><creatorcontrib>Imazawa, T</creatorcontrib><creatorcontrib>Tamura, T</creatorcontrib><creatorcontrib>Toyosawa, K</creatorcontrib><creatorcontrib>Shimada, A</creatorcontrib><creatorcontrib>Hirose, M</creatorcontrib><creatorcontrib>Mitsumori, K</creatorcontrib><title>Sequential observation of 2,6-dimethylaniline-induced nasal lesions in a rat two-stage nasal carcinogenesis model after initiation with N-bis(2-hydroxypropyl) nitrosamine</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Male F344 rats received diet containing 3,000 ppm 2,6-dimethylaniline (DMA) after initiation with a single subcutaneous injection of 2,400 mg/kg of N-bis(2-hydroxypropyl)nitrosamine (DHPN), and histological and electron microscopic examinations of the nasal cavity were performed at 4, 13, 26 and 52 weeks to examine sequential changes induced by DMA. Severe atrophy of Bowman's glands and epithelial disarrangement were apparent from week 4, followed by dilatation and/or proliferation of Bowman's glands, degeneration of epithelial cells, and proliferation of undifferentiated epithelial cells from week 13. Focal glandular hyperplasias, dysplastic foci, and adenomas were observed from week 26, and carcinomas at 52 week. These nasal lesions were mostly evident in the olfactory mucosa in the nasal cavity, and their severity and/or incidences, other than atrophy of Bowman's glands, increased with the treatment period. Electron microscopically, carcinoma cells demonstrated desmosomes, dense secretory granules identical to those in normal Bowman's glands, a basement membrane, and microvilli. These results suggest that Bowman's glands are the target of DMA, giving rise to nasal carcinomas after DHPN-initiation.</description><subject>2,6-dimethylaniline</subject><subject>Adenoma - chemically induced</subject><subject>Adenoma - pathology</subject><subject>Aniline Compounds</subject><subject>Animals</subject><subject>Atrophy</subject><subject>CARCINOGENESIS</subject><subject>carcinogenicity</subject><subject>Carcinogens</subject><subject>Carcinoma - chemically induced</subject><subject>Carcinoma - pathology</subject><subject>Cell Division</subject><subject>Cell Nucleus - pathology</subject><subject>Cell Nucleus - ultrastructure</subject><subject>HISTOCYTOLOGICAL ANALYSIS</subject><subject>Hyperplasia</subject><subject>Male</subject><subject>MUTAGENS</subject><subject>N-bis(2-hydroxypropyl)nitrosamine</subject><subject>nasal cavity</subject><subject>Nasal Mucosa - cytology</subject><subject>Nasal Mucosa - drug effects</subject><subject>Nasal Mucosa - pathology</subject><subject>NITROSAMINES</subject><subject>NOSE</subject><subject>Nose Neoplasms - chemically induced</subject><subject>Nose Neoplasms - pathology</subject><subject>Nose Neoplasms - ultrastructure</subject><subject>Olfactory Mucosa - cytology</subject><subject>Olfactory Mucosa - drug effects</subject><subject>Olfactory Mucosa - pathology</subject><subject>rat</subject><subject>RATS</subject><subject>Rats, Inbred F344</subject><subject>Time Factors</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkk-P0zAQxSMEYpeFE2eQJSQEghTbceLmBlrxVytAAs7WJJ60rhK72O4u_Up8SqabakFc7IN_82bmPRfFQ8EXQrby1eZySotGLnQtbhWnolK61KpqbxenvBVNqWXNT4p7KW04l0I17d3iRPBW1bJVp8Xvb_hzhz47GFnoEsZLyC54FgYmXzaldRPm9X4E70bnsXTe7nq0zEOighETsYk5z4BFyCxfhTJlWOER6CH2zocVeiITm4LFkcGQMVKNo6bXva5cXrPPZefSM1mu9zaGX_ttDNv9-JwRFUOCiZrfL-4MMCZ8cLzPih_v3n4__1BefHn_8fzNRdnXtchlt1w2rehBWUQ1IAwcbW3JFaiwV0vUoKVV_bBsll2lZdUC70ADMXywnW6rs-LprEszkDcpm8mlHkcyAcMuGcm1rrVUBD75D9yEXfQ0myGfSV82qiLqxUz1tEiKOJhtdBPEvRHcHAI0hwBNIw0FSPTjo-aum9D-w86JEfB6BjbXRt8AELPrR_wrNh-kefPUryEa9CTxaJYYIBhYRZfMp6-Sc3H4IGTAH2Mqu98</recordid><startdate>20000701</startdate><enddate>20000701</enddate><creator>Koujitani, T. (National Inst. of Health Science, Tokyo (Japan))</creator><creator>Yasuhara, K</creator><creator>Ikeda, T</creator><creator>Imazawa, T</creator><creator>Tamura, T</creator><creator>Toyosawa, K</creator><creator>Shimada, A</creator><creator>Hirose, M</creator><creator>Mitsumori, K</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20000701</creationdate><title>Sequential observation of 2,6-dimethylaniline-induced nasal lesions in a rat two-stage nasal carcinogenesis model after initiation with N-bis(2-hydroxypropyl) nitrosamine</title><author>Koujitani, T. 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(National Inst. of Health Science, Tokyo (Japan))</creatorcontrib><creatorcontrib>Yasuhara, K</creatorcontrib><creatorcontrib>Ikeda, T</creatorcontrib><creatorcontrib>Imazawa, T</creatorcontrib><creatorcontrib>Tamura, T</creatorcontrib><creatorcontrib>Toyosawa, K</creatorcontrib><creatorcontrib>Shimada, A</creatorcontrib><creatorcontrib>Hirose, M</creatorcontrib><creatorcontrib>Mitsumori, K</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koujitani, T. (National Inst. of Health Science, Tokyo (Japan))</au><au>Yasuhara, K</au><au>Ikeda, T</au><au>Imazawa, T</au><au>Tamura, T</au><au>Toyosawa, K</au><au>Shimada, A</au><au>Hirose, M</au><au>Mitsumori, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequential observation of 2,6-dimethylaniline-induced nasal lesions in a rat two-stage nasal carcinogenesis model after initiation with N-bis(2-hydroxypropyl) nitrosamine</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2000-07-01</date><risdate>2000</risdate><volume>62</volume><issue>7</issue><spage>751</spage><epage>756</epage><pages>751-756</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Male F344 rats received diet containing 3,000 ppm 2,6-dimethylaniline (DMA) after initiation with a single subcutaneous injection of 2,400 mg/kg of N-bis(2-hydroxypropyl)nitrosamine (DHPN), and histological and electron microscopic examinations of the nasal cavity were performed at 4, 13, 26 and 52 weeks to examine sequential changes induced by DMA. Severe atrophy of Bowman's glands and epithelial disarrangement were apparent from week 4, followed by dilatation and/or proliferation of Bowman's glands, degeneration of epithelial cells, and proliferation of undifferentiated epithelial cells from week 13. Focal glandular hyperplasias, dysplastic foci, and adenomas were observed from week 26, and carcinomas at 52 week. These nasal lesions were mostly evident in the olfactory mucosa in the nasal cavity, and their severity and/or incidences, other than atrophy of Bowman's glands, increased with the treatment period. Electron microscopically, carcinoma cells demonstrated desmosomes, dense secretory granules identical to those in normal Bowman's glands, a basement membrane, and microvilli. These results suggest that Bowman's glands are the target of DMA, giving rise to nasal carcinomas after DHPN-initiation.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>10945294</pmid><doi>10.1292/jvms.62.751</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Veterinary Medical Science, 2000, Vol.62(7), pp.751-756 |
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| language | eng |
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| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 2,6-dimethylaniline Adenoma - chemically induced Adenoma - pathology Aniline Compounds Animals Atrophy CARCINOGENESIS carcinogenicity Carcinogens Carcinoma - chemically induced Carcinoma - pathology Cell Division Cell Nucleus - pathology Cell Nucleus - ultrastructure HISTOCYTOLOGICAL ANALYSIS Hyperplasia Male MUTAGENS N-bis(2-hydroxypropyl)nitrosamine nasal cavity Nasal Mucosa - cytology Nasal Mucosa - drug effects Nasal Mucosa - pathology NITROSAMINES NOSE Nose Neoplasms - chemically induced Nose Neoplasms - pathology Nose Neoplasms - ultrastructure Olfactory Mucosa - cytology Olfactory Mucosa - drug effects Olfactory Mucosa - pathology rat RATS Rats, Inbred F344 Time Factors |
| title | Sequential observation of 2,6-dimethylaniline-induced nasal lesions in a rat two-stage nasal carcinogenesis model after initiation with N-bis(2-hydroxypropyl) nitrosamine |
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