A rapid method for the estimation of mean dry weight and lipid content of the infective juveniles of entomopathogenic nematodes using image analysis
AbstractA method using computer image analysis for the estimation of dry weight, lipid content, body size and length of individual infective juveniles (IJ) of entomopathogenic nematodes (EN) is described. Images of IJ, captured using a video camera, were digitalised and transferred to a computer for...
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description | AbstractA method using computer image analysis for the estimation of dry weight, lipid content, body size and length of individual infective juveniles (IJ) of entomopathogenic nematodes (EN) is described. Images of IJ, captured using a video camera, were digitalised and transferred to a computer for analysis. The longitudinal cross-sectional area of the nematode (Area), mean grey level (MGL) and perimeter of the nematode image (Perimeter) were measured automatically. Nematode samples having a range of sizes, mean dry weights and lipid contents, as well as a series of samples of different ages were examined. It was found that: i) the index Area x MGL correlates well with the mean dry weight of the nematode, which is in turn a good indicator of the total energy reserve of the nematodes; ii) MGL correlates well with the lipid content (as % dry weight) which is a good indicator of the quality of the energy reserve; and iii) the perimeter of the nematode image can be used to estimate the length of an IJ. Compared to currently available methods, such as direct measurement of mean dry weight or lipid content, computerised image analysis gives more information concerning the samples tested and enables variations in dry weight, lipid content, body size and length between individual IJ to be estimated readily. A sample of 30 IJ can be examined in 30 to 60 min making this method ideal for quality control during production, processing, formulation and storage of EN-based biopesticide products. Methode rapide pour l'estimation du poids sec et du contenu lipidique moyens des juveniles infestants de nematodes entomopathogenes basee sur l'analyse d'images - Il est decrit une methode utilisant l'analyse numerisee d'images et permettant l'estimation du poids sec, du contenu lipidique, de la taille et de la longueur du corps des juveniles infestants (IJ) de nematodes entomopathogenes. Les images des IJ, saisies par video-camera, sont digitalisees et transferees pour analyse sur ordinateur. La surface de la coupe longitudinale du nematode (Area), le niveau moyen d'opacite (MGL) et le perimetre de l'image du nematode (Perimeter) sont mesures automatiquement. Des echantillons de nematodes de taille, de poids sec et de contenu lipidique varies ainsi qu'une serie representant differents ages ont ete examines. Il a ete constate que: i) l'index Area x MGL est bien correle au poidsmoyen du nematode, lequel represente un bon indicateur de la reserve energetique totale du nematode; ii |
doi_str_mv | 10.1163/156854199508612 |
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Images of IJ, captured using a video camera, were digitalised and transferred to a computer for analysis. The longitudinal cross-sectional area of the nematode (Area), mean grey level (MGL) and perimeter of the nematode image (Perimeter) were measured automatically. Nematode samples having a range of sizes, mean dry weights and lipid contents, as well as a series of samples of different ages were examined. It was found that: i) the index Area x MGL correlates well with the mean dry weight of the nematode, which is in turn a good indicator of the total energy reserve of the nematodes; ii) MGL correlates well with the lipid content (as % dry weight) which is a good indicator of the quality of the energy reserve; and iii) the perimeter of the nematode image can be used to estimate the length of an IJ. Compared to currently available methods, such as direct measurement of mean dry weight or lipid content, computerised image analysis gives more information concerning the samples tested and enables variations in dry weight, lipid content, body size and length between individual IJ to be estimated readily. A sample of 30 IJ can be examined in 30 to 60 min making this method ideal for quality control during production, processing, formulation and storage of EN-based biopesticide products. Methode rapide pour l'estimation du poids sec et du contenu lipidique moyens des juveniles infestants de nematodes entomopathogenes basee sur l'analyse d'images - Il est decrit une methode utilisant l'analyse numerisee d'images et permettant l'estimation du poids sec, du contenu lipidique, de la taille et de la longueur du corps des juveniles infestants (IJ) de nematodes entomopathogenes. Les images des IJ, saisies par video-camera, sont digitalisees et transferees pour analyse sur ordinateur. La surface de la coupe longitudinale du nematode (Area), le niveau moyen d'opacite (MGL) et le perimetre de l'image du nematode (Perimeter) sont mesures automatiquement. Des echantillons de nematodes de taille, de poids sec et de contenu lipidique varies ainsi qu'une serie representant differents ages ont ete examines. Il a ete constate que: i) l'index Area x MGL est bien correle au poidsmoyen du nematode, lequel represente un bon indicateur de la reserve energetique totale du nematode; ii) MGL est bien correle au contenu lipidique (en % du poids sec), bon indicateur de la qualite de la reserve energetique; iii) le perimetre de l'image peut etre utilise pour estimer la longueur des IJ. Comparee aux methodes couramment utilisees- telle la mesure directe du poids sec et du contenu lipidique moyens - l'analyse numerisee des images donne une meilleure information sur les echantillons testes et permet une estimation rapide des variations individuelles du poids sec, du contenu lipidique, de la taille et de la longueur des IJ. Un echantillon de 30 IJ peut etre examine en 30 a 60 min ce qui rend cette methode ideale pour les controles de qualite pendant la production, les traitements, la formulation et le stockage des produits biopesticides a base de nematodes entomopathogenes.</description><identifier>ISSN: 1388-5545</identifier><identifier>EISSN: 1568-5411</identifier><identifier>DOI: 10.1163/156854199508612</identifier><language>eng</language><publisher>The Netherlands: Brill</publisher><subject>Biological and medical sciences ; body length ; body size ; chemical composition ; computer analysis ; ENERGY RESERVE ; entomopathogenic nematodes ; estimation ; Fundamental and applied biological sciences. Psychology ; General aspects and techniques. Study of several systematic groups. Models ; image analysis ; Invertebrates ; lipids ; Miscellaneous techniques ; Nematoda ; nematode larvae ; nutrient reserves ; QUALITY CONTROL ; quality controls ; rapid methods ; size ; STEINERNEMA CARPOCAPSAE ; Techniques ; weight</subject><ispartof>Nematology : international journal of fundamental and applied nematological research, 1999, Vol.1 (6), p.655-660</ispartof><rights>1999 Koninklijke Brill NV, Leiden, The Netherlands</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b399t-82b5db3af50171ae40592b5060ea7135fcbb6da825c3b907880cd57507b1e7a43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27921,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1221412$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiu, Lihong</creatorcontrib><creatorcontrib>Bedding, Robin</creatorcontrib><title>A rapid method for the estimation of mean dry weight and lipid content of the infective juveniles of entomopathogenic nematodes using image analysis</title><title>Nematology : international journal of fundamental and applied nematological research</title><addtitle>NEMY</addtitle><description>AbstractA method using computer image analysis for the estimation of dry weight, lipid content, body size and length of individual infective juveniles (IJ) of entomopathogenic nematodes (EN) is described. Images of IJ, captured using a video camera, were digitalised and transferred to a computer for analysis. The longitudinal cross-sectional area of the nematode (Area), mean grey level (MGL) and perimeter of the nematode image (Perimeter) were measured automatically. Nematode samples having a range of sizes, mean dry weights and lipid contents, as well as a series of samples of different ages were examined. It was found that: i) the index Area x MGL correlates well with the mean dry weight of the nematode, which is in turn a good indicator of the total energy reserve of the nematodes; ii) MGL correlates well with the lipid content (as % dry weight) which is a good indicator of the quality of the energy reserve; and iii) the perimeter of the nematode image can be used to estimate the length of an IJ. Compared to currently available methods, such as direct measurement of mean dry weight or lipid content, computerised image analysis gives more information concerning the samples tested and enables variations in dry weight, lipid content, body size and length between individual IJ to be estimated readily. A sample of 30 IJ can be examined in 30 to 60 min making this method ideal for quality control during production, processing, formulation and storage of EN-based biopesticide products. Methode rapide pour l'estimation du poids sec et du contenu lipidique moyens des juveniles infestants de nematodes entomopathogenes basee sur l'analyse d'images - Il est decrit une methode utilisant l'analyse numerisee d'images et permettant l'estimation du poids sec, du contenu lipidique, de la taille et de la longueur du corps des juveniles infestants (IJ) de nematodes entomopathogenes. Les images des IJ, saisies par video-camera, sont digitalisees et transferees pour analyse sur ordinateur. La surface de la coupe longitudinale du nematode (Area), le niveau moyen d'opacite (MGL) et le perimetre de l'image du nematode (Perimeter) sont mesures automatiquement. Des echantillons de nematodes de taille, de poids sec et de contenu lipidique varies ainsi qu'une serie representant differents ages ont ete examines. Il a ete constate que: i) l'index Area x MGL est bien correle au poidsmoyen du nematode, lequel represente un bon indicateur de la reserve energetique totale du nematode; ii) MGL est bien correle au contenu lipidique (en % du poids sec), bon indicateur de la qualite de la reserve energetique; iii) le perimetre de l'image peut etre utilise pour estimer la longueur des IJ. Comparee aux methodes couramment utilisees- telle la mesure directe du poids sec et du contenu lipidique moyens - l'analyse numerisee des images donne une meilleure information sur les echantillons testes et permet une estimation rapide des variations individuelles du poids sec, du contenu lipidique, de la taille et de la longueur des IJ. Un echantillon de 30 IJ peut etre examine en 30 a 60 min ce qui rend cette methode ideale pour les controles de qualite pendant la production, les traitements, la formulation et le stockage des produits biopesticides a base de nematodes entomopathogenes.</description><subject>Biological and medical sciences</subject><subject>body length</subject><subject>body size</subject><subject>chemical composition</subject><subject>computer analysis</subject><subject>ENERGY RESERVE</subject><subject>entomopathogenic nematodes</subject><subject>estimation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>image analysis</subject><subject>Invertebrates</subject><subject>lipids</subject><subject>Miscellaneous techniques</subject><subject>Nematoda</subject><subject>nematode larvae</subject><subject>nutrient reserves</subject><subject>QUALITY CONTROL</subject><subject>quality controls</subject><subject>rapid methods</subject><subject>size</subject><subject>STEINERNEMA CARPOCAPSAE</subject><subject>Techniques</subject><subject>weight</subject><issn>1388-5545</issn><issn>1568-5411</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNp1kEtv1DAUhSMEEqWwZokXiF1aP-IkXlYDpZQiFkzF0rpxnIyHjD3YTmH-Bz-YG6aiEhIrW_d859xHUbxk9IyxWpwzWbeyYkpJ2taMPypOlkqJJfYY_6LFv6zk0-JZSltKqVSSnRS_LkiEvevJzuZN6MkQIskbS2zKbgfZBU_CgCJ40scD-WHduMkEfE8mt9hM8Nn6vECLzfnBmuzuLNnOd9a7yaZFQiLswh6wxYhVQ7zF8NCjOifnR4K9RouxMB2SS8-LJwNMyb64f0-L28t369VVefP5_YfVxU3ZCaVy2fJO9p2AQVLWMLAV7oQlWlMLDRNyMF1X99ByaUSnaNO21PSykbTpmG2gEqfFm2PuPobvM66sdy4ZO03gbZiT5rSpKWs5gudH0MSQUrSD3kccOR40o3q5vv7n-uh4fR8NycA0RPDGpQcb56z6g5VHzKVsf_6VIX7TdSMaqa8_rvXq09tLtabX-ivyr478AEHDGDHy9gunTFCuOFeKPSR20U2T3oY54lXTfwf9DbEDq_c</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Qiu, Lihong</creator><creator>Bedding, Robin</creator><general>Brill</general><general>BRILL</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7SS</scope><scope>C1K</scope></search><sort><creationdate>1999</creationdate><title>A rapid method for the estimation of mean dry weight and lipid content of the infective juveniles of entomopathogenic nematodes using image analysis</title><author>Qiu, Lihong ; Bedding, Robin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b399t-82b5db3af50171ae40592b5060ea7135fcbb6da825c3b907880cd57507b1e7a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>body length</topic><topic>body size</topic><topic>chemical composition</topic><topic>computer analysis</topic><topic>ENERGY RESERVE</topic><topic>entomopathogenic nematodes</topic><topic>estimation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>image analysis</topic><topic>Invertebrates</topic><topic>lipids</topic><topic>Miscellaneous techniques</topic><topic>Nematoda</topic><topic>nematode larvae</topic><topic>nutrient reserves</topic><topic>QUALITY CONTROL</topic><topic>quality controls</topic><topic>rapid methods</topic><topic>size</topic><topic>STEINERNEMA CARPOCAPSAE</topic><topic>Techniques</topic><topic>weight</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiu, Lihong</creatorcontrib><creatorcontrib>Bedding, Robin</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Nematology : international journal of fundamental and applied nematological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiu, Lihong</au><au>Bedding, Robin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid method for the estimation of mean dry weight and lipid content of the infective juveniles of entomopathogenic nematodes using image analysis</atitle><jtitle>Nematology : international journal of fundamental and applied nematological research</jtitle><addtitle>NEMY</addtitle><date>1999</date><risdate>1999</risdate><volume>1</volume><issue>6</issue><spage>655</spage><epage>660</epage><pages>655-660</pages><issn>1388-5545</issn><eissn>1568-5411</eissn><abstract>AbstractA method using computer image analysis for the estimation of dry weight, lipid content, body size and length of individual infective juveniles (IJ) of entomopathogenic nematodes (EN) is described. Images of IJ, captured using a video camera, were digitalised and transferred to a computer for analysis. The longitudinal cross-sectional area of the nematode (Area), mean grey level (MGL) and perimeter of the nematode image (Perimeter) were measured automatically. Nematode samples having a range of sizes, mean dry weights and lipid contents, as well as a series of samples of different ages were examined. It was found that: i) the index Area x MGL correlates well with the mean dry weight of the nematode, which is in turn a good indicator of the total energy reserve of the nematodes; ii) MGL correlates well with the lipid content (as % dry weight) which is a good indicator of the quality of the energy reserve; and iii) the perimeter of the nematode image can be used to estimate the length of an IJ. Compared to currently available methods, such as direct measurement of mean dry weight or lipid content, computerised image analysis gives more information concerning the samples tested and enables variations in dry weight, lipid content, body size and length between individual IJ to be estimated readily. A sample of 30 IJ can be examined in 30 to 60 min making this method ideal for quality control during production, processing, formulation and storage of EN-based biopesticide products. Methode rapide pour l'estimation du poids sec et du contenu lipidique moyens des juveniles infestants de nematodes entomopathogenes basee sur l'analyse d'images - Il est decrit une methode utilisant l'analyse numerisee d'images et permettant l'estimation du poids sec, du contenu lipidique, de la taille et de la longueur du corps des juveniles infestants (IJ) de nematodes entomopathogenes. Les images des IJ, saisies par video-camera, sont digitalisees et transferees pour analyse sur ordinateur. La surface de la coupe longitudinale du nematode (Area), le niveau moyen d'opacite (MGL) et le perimetre de l'image du nematode (Perimeter) sont mesures automatiquement. Des echantillons de nematodes de taille, de poids sec et de contenu lipidique varies ainsi qu'une serie representant differents ages ont ete examines. Il a ete constate que: i) l'index Area x MGL est bien correle au poidsmoyen du nematode, lequel represente un bon indicateur de la reserve energetique totale du nematode; ii) MGL est bien correle au contenu lipidique (en % du poids sec), bon indicateur de la qualite de la reserve energetique; iii) le perimetre de l'image peut etre utilise pour estimer la longueur des IJ. Comparee aux methodes couramment utilisees- telle la mesure directe du poids sec et du contenu lipidique moyens - l'analyse numerisee des images donne une meilleure information sur les echantillons testes et permet une estimation rapide des variations individuelles du poids sec, du contenu lipidique, de la taille et de la longueur des IJ. Un echantillon de 30 IJ peut etre examine en 30 a 60 min ce qui rend cette methode ideale pour les controles de qualite pendant la production, les traitements, la formulation et le stockage des produits biopesticides a base de nematodes entomopathogenes.</abstract><cop>The Netherlands</cop><pub>Brill</pub><doi>10.1163/156854199508612</doi><tpages>6</tpages></addata></record> |
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subjects | Biological and medical sciences body length body size chemical composition computer analysis ENERGY RESERVE entomopathogenic nematodes estimation Fundamental and applied biological sciences. Psychology General aspects and techniques. Study of several systematic groups. Models image analysis Invertebrates lipids Miscellaneous techniques Nematoda nematode larvae nutrient reserves QUALITY CONTROL quality controls rapid methods size STEINERNEMA CARPOCAPSAE Techniques weight |
title | A rapid method for the estimation of mean dry weight and lipid content of the infective juveniles of entomopathogenic nematodes using image analysis |
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