Analysis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis
Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) were applied to the simultaneous separation of cyanobacterial toxins (anatoxin‐a, microcystin‐LR, cylindrospermopsin). The analytical performance data of both methods, optimized for the three toxins, were similar...
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| Veröffentlicht in: | Electrophoresis 2004-01, Vol.25 (1), p.108-115 |
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| creator | Vasas, Gábor Gáspár, Attila Páger, Csilla Surányi, Gyula Máthé, Csaba Hamvas, Márta M. Borbely, George |
| description | Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) were applied to the simultaneous separation of cyanobacterial toxins (anatoxin‐a, microcystin‐LR, cylindrospermopsin). The analytical performance data of both methods, optimized for the three toxins, were similar with a precision of migration times smaller than 0.8 RSD% and a detection limit in the range of 1–4 μg/mL, using spectrophotometric detection at 230 nm. Both methods were applied to an analysis of cyanotoxins in water bloom samples and crude cyanobacterial extracts. The results obtained indicate that, for complex matrices, the sequential application of CZE and MEKC is necessary. It is recommended to use both CE techniques for the analysis of the same sample in order to confirm the results by an orthogonal approach. |
| doi_str_mv | 10.1002/elps.200305641 |
| format | Article |
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The analytical performance data of both methods, optimized for the three toxins, were similar with a precision of migration times smaller than 0.8 RSD% and a detection limit in the range of 1–4 μg/mL, using spectrophotometric detection at 230 nm. Both methods were applied to an analysis of cyanotoxins in water bloom samples and crude cyanobacterial extracts. The results obtained indicate that, for complex matrices, the sequential application of CZE and MEKC is necessary. It is recommended to use both CE techniques for the analysis of the same sample in order to confirm the results by an orthogonal approach.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.200305641</identifier><identifier>PMID: 14730574</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Anatoxin-a ; Bacterial Toxins - analysis ; Bacterial Toxins - isolation & purification ; Capillary electrophoresis ; Cyanobacterial toxins ; Cylindrospermopsin ; Electrophoresis, Capillary - methods ; Electrophoresis, Capillary - standards ; Marine Toxins - analysis ; Marine Toxins - isolation & purification ; Microcystin-LR ; Microcystins ; Peptides, Cyclic - analysis ; Peptides, Cyclic - isolation & purification ; Tropanes ; Uracil - analogs & derivatives ; Uracil - analysis ; Uracil - isolation & purification ; Water Pollutants - analysis</subject><ispartof>Electrophoresis, 2004-01, Vol.25 (1), p.108-115</ispartof><rights>Copyright © 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4101-57025a880d788f88a9bfecacafdc3718aa9811eaf16c030b32f88477ffdb02eb3</citedby><cites>FETCH-LOGICAL-c4101-57025a880d788f88a9bfecacafdc3718aa9811eaf16c030b32f88477ffdb02eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felps.200305641$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27928,27929,45579</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14730574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vasas, Gábor</creatorcontrib><creatorcontrib>Gáspár, Attila</creatorcontrib><creatorcontrib>Páger, Csilla</creatorcontrib><creatorcontrib>Surányi, Gyula</creatorcontrib><creatorcontrib>Máthé, Csaba</creatorcontrib><creatorcontrib>Hamvas, Márta M.</creatorcontrib><creatorcontrib>Borbely, George</creatorcontrib><title>Analysis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis</title><title>Electrophoresis</title><addtitle>ELECTROPHORESIS</addtitle><description>Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) were applied to the simultaneous separation of cyanobacterial toxins (anatoxin‐a, microcystin‐LR, cylindrospermopsin). The analytical performance data of both methods, optimized for the three toxins, were similar with a precision of migration times smaller than 0.8 RSD% and a detection limit in the range of 1–4 μg/mL, using spectrophotometric detection at 230 nm. Both methods were applied to an analysis of cyanotoxins in water bloom samples and crude cyanobacterial extracts. The results obtained indicate that, for complex matrices, the sequential application of CZE and MEKC is necessary. It is recommended to use both CE techniques for the analysis of the same sample in order to confirm the results by an orthogonal approach.</description><subject>Anatoxin-a</subject><subject>Bacterial Toxins - analysis</subject><subject>Bacterial Toxins - isolation & purification</subject><subject>Capillary electrophoresis</subject><subject>Cyanobacterial toxins</subject><subject>Cylindrospermopsin</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Electrophoresis, Capillary - standards</subject><subject>Marine Toxins - analysis</subject><subject>Marine Toxins - isolation & purification</subject><subject>Microcystin-LR</subject><subject>Microcystins</subject><subject>Peptides, Cyclic - analysis</subject><subject>Peptides, Cyclic - isolation & purification</subject><subject>Tropanes</subject><subject>Uracil - analogs & derivatives</subject><subject>Uracil - analysis</subject><subject>Uracil - isolation & purification</subject><subject>Water Pollutants - analysis</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1v1DAQxS0EotvClSPKCbVSs_grsXOs-gXqCipYxNGaOLZw68SpnVWb_75ud1W4cZo5_N6beQ-hDwQvCcb0s_FjWlKMGa5qTl6hBakoLWkt2Wu0wESwEktW7aH9lG4wxrzh_C3aI1xkgeALFE4G8HNyqQi20DMMoQU9mejAF1N4cEMqDmGA57WE44x4N3QxpNHEPozJDcdF73QMek5TRlY_jop2LjSMznuIc2G80VMM458QTT7zDr2x4JN5v5sH6NfF-fr0S7n6fvn19GRVak4wKSuBaQVS4k5IaaWEprVGgwbbaSaIBGgkIQYsqXXO3jKaIS6EtV2LqWnZAfq09R1juNuYNKneJW3yT4MJm6QoFlXDa5bB5RbMGVKKxqoxuj5_rghWTxWrp4rVS8VZ8HHnvGl70_3Fd51moNkC986b-T926nx1_fNf83KrdWkyDy9aiLeqFkxU6ve3S7WWZ-srciXVNXsEKoiakg</recordid><startdate>200401</startdate><enddate>200401</enddate><creator>Vasas, Gábor</creator><creator>Gáspár, Attila</creator><creator>Páger, Csilla</creator><creator>Surányi, Gyula</creator><creator>Máthé, Csaba</creator><creator>Hamvas, Márta M.</creator><creator>Borbely, George</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>200401</creationdate><title>Analysis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis</title><author>Vasas, Gábor ; 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| subjects | Anatoxin-a Bacterial Toxins - analysis Bacterial Toxins - isolation & purification Capillary electrophoresis Cyanobacterial toxins Cylindrospermopsin Electrophoresis, Capillary - methods Electrophoresis, Capillary - standards Marine Toxins - analysis Marine Toxins - isolation & purification Microcystin-LR Microcystins Peptides, Cyclic - analysis Peptides, Cyclic - isolation & purification Tropanes Uracil - analogs & derivatives Uracil - analysis Uracil - isolation & purification Water Pollutants - analysis |
| title | Analysis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis |
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