Escherichia coli O26 and O113:H21 on Carcasses and Beef from a Slaughterhouse Located in Mato Grosso, Brazil

Shiga toxin-producing Escherichia coli (STEC) is a group of emerging pathogens that can cause human diseases, including hemolytic uremic syndrome (HUS) and hemorrhagic colitis (HC). Monitoring slaughtering stages and checking contamination points are crucial for the production of safe food. In this...

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Veröffentlicht in:Foodborne pathogens and disease 2018-10, Vol.15 (10), p.653-659
Hauptverfasser: Santos, Elis Caroline Celestina Dos, Castro, Vinicius Silva, Cunha-Neto, Adelino, Santos, Luis Fernando Dos, Vallim, Deyse Christina, Lisbôa, Rodrigo de Castro, Carvalho, Ricardo César Tavares, Junior, Carlos Adam Conte, Figueiredo, Eduardo Eustáquio de Souza
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container_issue 10
container_start_page 653
container_title Foodborne pathogens and disease
container_volume 15
creator Santos, Elis Caroline Celestina Dos
Castro, Vinicius Silva
Cunha-Neto, Adelino
Santos, Luis Fernando Dos
Vallim, Deyse Christina
Lisbôa, Rodrigo de Castro
Carvalho, Ricardo César Tavares
Junior, Carlos Adam Conte
Figueiredo, Eduardo Eustáquio de Souza
description Shiga toxin-producing Escherichia coli (STEC) is a group of emerging pathogens that can cause human diseases, including hemolytic uremic syndrome (HUS) and hemorrhagic colitis (HC). Monitoring slaughtering stages and checking contamination points are crucial for the production of safe food. In this context, the aim of this study was to verify contamination by STEC strains, to determine the contamination points and evaluate the resistance profile to 12 antimicrobials used in both veterinary and human medicine. A total of 80 samples were obtained from eight collection points (pen floor, rectum, hide, carcass swabs and esophagus, diaphragm, masseter, and retail beef tissue samples). The isolates were collected by dilution plating on MacConkey agar with sorbitol, cefixime, and tellurite and analyzed by multiplex polymerase chain reaction for virulence genes. Serotyping of non-O157 was performed, and testing for 12 antibiotics by disk diffusion was carried out. A total of 18 STEC strains were isolated, presenting different virulence profiles. Contamination by STEC was observed in the rectum (5/18), carcass surface (5/18), hide (3/18), diaphragm (2/18), retail beef (2/18), and masseter muscle (1/18). Pen floor swabs and esophagus tissues showed no STEC contamination. Moreover, three strains were identified as O26 and three as O113:H21 strains, which have been linked to HUS and HC outbreak cases in Brazil. All STEC isolates were susceptible to all evaluated antimicrobials, except streptomycin. The presence of STEC strains is a direct risk to the consumer, especially when isolated from retail beef, and contamination can occur during different slaughter stages. However, antimicrobial resistance profiles did not identify multidrug-resistant strains, limiting potential antimicrobial resistance transmission to other pathogens.
doi_str_mv 10.1089/fpd.2018.2431
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title Escherichia coli O26 and O113:H21 on Carcasses and Beef from a Slaughterhouse Located in Mato Grosso, Brazil
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