N-terminal engineering of overlapping genes in the nitrile hydratase gene cluster improved its activity

•A fusion tag strategy was applied to tune the expression of activator.•A silent mutation strategy was applied to improve the expression of β-subunit.•The expression levels of three ORFs were balanced and the final activity improved. Nitrile hydratase which catalyzes the hydration of nitriles to the...

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Veröffentlicht in:	Enzyme and microbial technology 2018-10, Vol.117, p.9-14
Hauptverfasser:	Yang, Zhengfei, Pei, Xiaolin, Xu, Gang, Wu, Jianping, Yang, Lirong
Format:	Artikel
Sprache:	eng
Schlagworte:	Activator Alphaproteobacteria - enzymology Alphaproteobacteria - genetics Base Sequence Fusion tag Gene cluster Gene Expression Regulation, Bacterial Genes, Overlapping Genetic Engineering - methods Hydro-Lyases - genetics Hydro-Lyases - metabolism Multigene Family Nitrile hydratase Operon Silent Mutation
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