Commercial cannabis consumer products part 2: HPLC-DAD quantitative analysis of cannabis cannabinoids
•HPLC-DAD cannabinoids quantitation in large variety of commercial cannabis products.•Foods, candies, beverages, topicals, vapes/eliquids, oral supplements.•11 cannabis cannabinoids resolved with mixed C18-aromatic stationary phase.•Extensive method validation for CBD, Δ9-THC, CBDA, THCA, and CBN. Q...
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| Veröffentlicht in: | Forensic science international 2018-08, Vol.289, p.438-447 |
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| creator | Ciolino, Laura A. Ranieri, Tracy L. Taylor, Allison M. |
| description | •HPLC-DAD cannabinoids quantitation in large variety of commercial cannabis products.•Foods, candies, beverages, topicals, vapes/eliquids, oral supplements.•11 cannabis cannabinoids resolved with mixed C18-aromatic stationary phase.•Extensive method validation for CBD, Δ9-THC, CBDA, THCA, and CBN.
Quantitative analysis for the cannabis cannabinoids such as cannabidiol and Δ9-tetrahydrocannabinol in commercial products is necessary for evaluating label information, and assessing dosages and exposures when the products are consumed. Herein is presented a broadly applicable HPLC-DAD method for the determination of cannabis cannabinoids in commercial consumer products and traditional plant-related substances. The current method provides chromatographic resolution of 11 cannabinoids using a commercial, mixed C18-aromatic functionality stationary phase. The method uses 95% or pure ethanol for extraction, and certain modifications which address specific matrix types are detailed herein. Extensive method validation including precision and accuracy was conducted for five cannabinoids of primary interest (CBD, Δ9-THC, CBDA, THCA, and CBN). UV detection provided excellent sensitivity with limits of quantitation (LOQs) of 10μg/g across cannabinoids. The method was applied to about 60 commercial products representing diverse product types and a broad range of cannabinoids amounts (0.01–350mg/g). |
| doi_str_mv | 10.1016/j.forsciint.2018.05.033 |
| format | Article |
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Quantitative analysis for the cannabis cannabinoids such as cannabidiol and Δ9-tetrahydrocannabinol in commercial products is necessary for evaluating label information, and assessing dosages and exposures when the products are consumed. Herein is presented a broadly applicable HPLC-DAD method for the determination of cannabis cannabinoids in commercial consumer products and traditional plant-related substances. The current method provides chromatographic resolution of 11 cannabinoids using a commercial, mixed C18-aromatic functionality stationary phase. The method uses 95% or pure ethanol for extraction, and certain modifications which address specific matrix types are detailed herein. Extensive method validation including precision and accuracy was conducted for five cannabinoids of primary interest (CBD, Δ9-THC, CBDA, THCA, and CBN). UV detection provided excellent sensitivity with limits of quantitation (LOQs) of 10μg/g across cannabinoids. The method was applied to about 60 commercial products representing diverse product types and a broad range of cannabinoids amounts (0.01–350mg/g).</description><identifier>ISSN: 0379-0738</identifier><identifier>EISSN: 1872-6283</identifier><identifier>DOI: 10.1016/j.forsciint.2018.05.033</identifier><identifier>PMID: 30025568</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Acids ; Analgesics ; Beverages ; Candy ; Cannabidiol ; Cannabinoids ; Cannabinoids - analysis ; Cannabis ; Cannabis cannabinoids ; Chromatography, High Pressure Liquid - methods ; Coffee ; Commercial cannabis products ; Consumer products ; Dronabinol - chemistry ; Ethanol ; Food ; Forensic sciences ; Hemp ; High performance liquid chromatography ; High performance liquid chromatography-diode array detection (HPLC-DAD) ; Humans ; Laboratories ; Liquid chromatography ; Marijuana ; Methods ; Nonsteroidal anti-inflammatory drugs ; Plant Oils - chemistry ; Plant Preparations - chemistry ; Quantitation ; Quantitative analysis ; Seeds ; Skin Cream - chemistry ; Stationary phase ; Tetrahydrocannabinol ; Ultraviolet radiation ; Δ9-tetrahydrocannabinol</subject><ispartof>Forensic science international, 2018-08, Vol.289, p.438-447</ispartof><rights>2018</rights><rights>Published by Elsevier B.V.</rights><rights>Copyright Elsevier Limited Aug 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-f193b2c4f2c671f5ad07d80c8c5fb2fd739231cb7ca00d6a270c88d8cc5063f33</citedby><cites>FETCH-LOGICAL-c465t-f193b2c4f2c671f5ad07d80c8c5fb2fd739231cb7ca00d6a270c88d8cc5063f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0379073818302858$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30025568$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ciolino, Laura A.</creatorcontrib><creatorcontrib>Ranieri, Tracy L.</creatorcontrib><creatorcontrib>Taylor, Allison M.</creatorcontrib><title>Commercial cannabis consumer products part 2: HPLC-DAD quantitative analysis of cannabis cannabinoids</title><title>Forensic science international</title><addtitle>Forensic Sci Int</addtitle><description>•HPLC-DAD cannabinoids quantitation in large variety of commercial cannabis products.•Foods, candies, beverages, topicals, vapes/eliquids, oral supplements.•11 cannabis cannabinoids resolved with mixed C18-aromatic stationary phase.•Extensive method validation for CBD, Δ9-THC, CBDA, THCA, and CBN.
Quantitative analysis for the cannabis cannabinoids such as cannabidiol and Δ9-tetrahydrocannabinol in commercial products is necessary for evaluating label information, and assessing dosages and exposures when the products are consumed. Herein is presented a broadly applicable HPLC-DAD method for the determination of cannabis cannabinoids in commercial consumer products and traditional plant-related substances. The current method provides chromatographic resolution of 11 cannabinoids using a commercial, mixed C18-aromatic functionality stationary phase. The method uses 95% or pure ethanol for extraction, and certain modifications which address specific matrix types are detailed herein. Extensive method validation including precision and accuracy was conducted for five cannabinoids of primary interest (CBD, Δ9-THC, CBDA, THCA, and CBN). UV detection provided excellent sensitivity with limits of quantitation (LOQs) of 10μg/g across cannabinoids. The method was applied to about 60 commercial products representing diverse product types and a broad range of cannabinoids amounts (0.01–350mg/g).</description><subject>Acids</subject><subject>Analgesics</subject><subject>Beverages</subject><subject>Candy</subject><subject>Cannabidiol</subject><subject>Cannabinoids</subject><subject>Cannabinoids - analysis</subject><subject>Cannabis</subject><subject>Cannabis cannabinoids</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Coffee</subject><subject>Commercial cannabis products</subject><subject>Consumer products</subject><subject>Dronabinol - chemistry</subject><subject>Ethanol</subject><subject>Food</subject><subject>Forensic sciences</subject><subject>Hemp</subject><subject>High performance liquid chromatography</subject><subject>High performance liquid chromatography-diode array detection (HPLC-DAD)</subject><subject>Humans</subject><subject>Laboratories</subject><subject>Liquid chromatography</subject><subject>Marijuana</subject><subject>Methods</subject><subject>Nonsteroidal anti-inflammatory drugs</subject><subject>Plant Oils - chemistry</subject><subject>Plant Preparations - chemistry</subject><subject>Quantitation</subject><subject>Quantitative analysis</subject><subject>Seeds</subject><subject>Skin Cream - chemistry</subject><subject>Stationary phase</subject><subject>Tetrahydrocannabinol</subject><subject>Ultraviolet radiation</subject><subject>Δ9-tetrahydrocannabinol</subject><issn>0379-0738</issn><issn>1872-6283</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUtvEzEUhS0EoqHwF2AkNmxmuLbjR9hFKaVIkWABa8vjh-Roxk5tT6X--zqkrRAbVrbs75xrn4PQBwwDBsw_HwafcjEhxDoQwHIANgClL9AKS0F6TiR9iVZAxaYHQeUFelPKAQAYI_w1uqAAhDEuV8jt0jy7bIKeOqNj1GMonUmxLO20O-ZkF1NLd9S5duRLd_Nzv-uvtlfd7aJjDVXXcOc6HfV0X5ow-b9MzpuYgi1v0Suvp-LePa6X6Pf111-7m37_49v33XbfmzVntfd4Q0di1p4YLrBn2oKwEow0zI_EW0E3hGIzCqMBLNdEtDtppTEMOPWUXqJPZ9_28NvFlarmUIybJh1dWooiLQxKMJGsoR__QQ9pye0jJ4pvxBr4H0qcKZNTKdl5dcxh1vleYVCnJtRBPTehTk0oYKo10ZTvH_2XcXb2WfcUfQO2Z8C1QO6Cy6q5uGicDdmZqmwK_x3yAKxjnpQ</recordid><startdate>201808</startdate><enddate>201808</enddate><creator>Ciolino, Laura A.</creator><creator>Ranieri, Tracy L.</creator><creator>Taylor, Allison M.</creator><general>Elsevier B.V</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>201808</creationdate><title>Commercial cannabis consumer products part 2: HPLC-DAD quantitative analysis of cannabis cannabinoids</title><author>Ciolino, Laura A. ; Ranieri, Tracy L. ; Taylor, Allison M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-f193b2c4f2c671f5ad07d80c8c5fb2fd739231cb7ca00d6a270c88d8cc5063f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acids</topic><topic>Analgesics</topic><topic>Beverages</topic><topic>Candy</topic><topic>Cannabidiol</topic><topic>Cannabinoids</topic><topic>Cannabinoids - analysis</topic><topic>Cannabis</topic><topic>Cannabis cannabinoids</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Coffee</topic><topic>Commercial cannabis products</topic><topic>Consumer products</topic><topic>Dronabinol - chemistry</topic><topic>Ethanol</topic><topic>Food</topic><topic>Forensic sciences</topic><topic>Hemp</topic><topic>High performance liquid chromatography</topic><topic>High performance liquid chromatography-diode array detection (HPLC-DAD)</topic><topic>Humans</topic><topic>Laboratories</topic><topic>Liquid chromatography</topic><topic>Marijuana</topic><topic>Methods</topic><topic>Nonsteroidal anti-inflammatory drugs</topic><topic>Plant Oils - chemistry</topic><topic>Plant Preparations - chemistry</topic><topic>Quantitation</topic><topic>Quantitative analysis</topic><topic>Seeds</topic><topic>Skin Cream - chemistry</topic><topic>Stationary phase</topic><topic>Tetrahydrocannabinol</topic><topic>Ultraviolet radiation</topic><topic>Δ9-tetrahydrocannabinol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ciolino, Laura A.</creatorcontrib><creatorcontrib>Ranieri, Tracy L.</creatorcontrib><creatorcontrib>Taylor, Allison M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Forensic science international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ciolino, Laura A.</au><au>Ranieri, Tracy L.</au><au>Taylor, Allison M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Commercial cannabis consumer products part 2: HPLC-DAD quantitative analysis of cannabis cannabinoids</atitle><jtitle>Forensic science international</jtitle><addtitle>Forensic Sci Int</addtitle><date>2018-08</date><risdate>2018</risdate><volume>289</volume><spage>438</spage><epage>447</epage><pages>438-447</pages><issn>0379-0738</issn><eissn>1872-6283</eissn><abstract>•HPLC-DAD cannabinoids quantitation in large variety of commercial cannabis products.•Foods, candies, beverages, topicals, vapes/eliquids, oral supplements.•11 cannabis cannabinoids resolved with mixed C18-aromatic stationary phase.•Extensive method validation for CBD, Δ9-THC, CBDA, THCA, and CBN.
Quantitative analysis for the cannabis cannabinoids such as cannabidiol and Δ9-tetrahydrocannabinol in commercial products is necessary for evaluating label information, and assessing dosages and exposures when the products are consumed. Herein is presented a broadly applicable HPLC-DAD method for the determination of cannabis cannabinoids in commercial consumer products and traditional plant-related substances. The current method provides chromatographic resolution of 11 cannabinoids using a commercial, mixed C18-aromatic functionality stationary phase. The method uses 95% or pure ethanol for extraction, and certain modifications which address specific matrix types are detailed herein. Extensive method validation including precision and accuracy was conducted for five cannabinoids of primary interest (CBD, Δ9-THC, CBDA, THCA, and CBN). UV detection provided excellent sensitivity with limits of quantitation (LOQs) of 10μg/g across cannabinoids. The method was applied to about 60 commercial products representing diverse product types and a broad range of cannabinoids amounts (0.01–350mg/g).</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>30025568</pmid><doi>10.1016/j.forsciint.2018.05.033</doi><tpages>10</tpages></addata></record> |
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| ispartof | Forensic science international, 2018-08, Vol.289, p.438-447 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acids Analgesics Beverages Candy Cannabidiol Cannabinoids Cannabinoids - analysis Cannabis Cannabis cannabinoids Chromatography, High Pressure Liquid - methods Coffee Commercial cannabis products Consumer products Dronabinol - chemistry Ethanol Food Forensic sciences Hemp High performance liquid chromatography High performance liquid chromatography-diode array detection (HPLC-DAD) Humans Laboratories Liquid chromatography Marijuana Methods Nonsteroidal anti-inflammatory drugs Plant Oils - chemistry Plant Preparations - chemistry Quantitation Quantitative analysis Seeds Skin Cream - chemistry Stationary phase Tetrahydrocannabinol Ultraviolet radiation Δ9-tetrahydrocannabinol |
| title | Commercial cannabis consumer products part 2: HPLC-DAD quantitative analysis of cannabis cannabinoids |
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