Determination of relationships among non-toxigenic Vibrio cholerae O1 biotype El Tor strains from housekeeping gene sequences and ribotype patterns
Sequencing of three housekeeping genes, mdh, dnaE and recA, and ribotyping for seven non-toxigenic Vibrio cholerae O1 strains isolated from different geographic sources indicate a phylogenetic relationship among the strains. Results of MLST and ribotyping indicate a clear difference between three to...
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| Veröffentlicht in: | Research in microbiology 2009-01, Vol.160 (1), p.57-62 |
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| creator | Mohapatra, Saswat S. Ramachandran, Dhanya Mantri, Chinmay K. Colwell, Rita R. Singh, Durg V. |
| description | Sequencing of three housekeeping genes,
mdh,
dnaE and
recA, and ribotyping for seven non-toxigenic
Vibrio cholerae O1 strains isolated from different geographic sources indicate a phylogenetic relationship among the strains. Results of MLST and ribotyping indicate a clear difference between three toxigenic strains (N16961, O395, and 569B) and three non-toxigenic strains from India (GS1, GS2, and GW87) and one Guam strain (X392), the latter of which were similar in both MLST and ribotyping, while two other non-toxigenic strains from the USA and India (2740-80 and OR69) appeared to be more closely related to toxigenic strains than to non-toxigenic strains, although this was not supported by ribotyping. These results provide clues to the emergence of toxigenic strains from a non-toxigenic progenitor by acquisition of virulence gene clusters. Results of split decomposition analysis suggest that widespread recombination occurs among the three housekeeping genes and that recombination plays an important role in the emergence of toxigenic strains of
V. cholerae O1. |
| doi_str_mv | 10.1016/j.resmic.2008.10.008 |
| format | Article |
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mdh,
dnaE and
recA, and ribotyping for seven non-toxigenic
Vibrio cholerae O1 strains isolated from different geographic sources indicate a phylogenetic relationship among the strains. Results of MLST and ribotyping indicate a clear difference between three toxigenic strains (N16961, O395, and 569B) and three non-toxigenic strains from India (GS1, GS2, and GW87) and one Guam strain (X392), the latter of which were similar in both MLST and ribotyping, while two other non-toxigenic strains from the USA and India (2740-80 and OR69) appeared to be more closely related to toxigenic strains than to non-toxigenic strains, although this was not supported by ribotyping. These results provide clues to the emergence of toxigenic strains from a non-toxigenic progenitor by acquisition of virulence gene clusters. Results of split decomposition analysis suggest that widespread recombination occurs among the three housekeeping genes and that recombination plays an important role in the emergence of toxigenic strains of
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mdh,
dnaE and
recA, and ribotyping for seven non-toxigenic
Vibrio cholerae O1 strains isolated from different geographic sources indicate a phylogenetic relationship among the strains. Results of MLST and ribotyping indicate a clear difference between three toxigenic strains (N16961, O395, and 569B) and three non-toxigenic strains from India (GS1, GS2, and GW87) and one Guam strain (X392), the latter of which were similar in both MLST and ribotyping, while two other non-toxigenic strains from the USA and India (2740-80 and OR69) appeared to be more closely related to toxigenic strains than to non-toxigenic strains, although this was not supported by ribotyping. These results provide clues to the emergence of toxigenic strains from a non-toxigenic progenitor by acquisition of virulence gene clusters. Results of split decomposition analysis suggest that widespread recombination occurs among the three housekeeping genes and that recombination plays an important role in the emergence of toxigenic strains of
V. cholerae O1.</description><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA Polymerase III - genetics</subject><subject>DNA, Bacterial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetic Variation</subject><subject>Housekeeping genes</subject><subject>Malate Dehydrogenase - genetics</subject><subject>Marine</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Phylogeny</subject><subject>Rec A Recombinases - genetics</subject><subject>Recombination, Genetic</subject><subject>Ribotyping</subject><subject>Sequence Analysis, DNA</subject><subject>Sequencing</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae O1 - classification</subject><subject>Vibrio cholerae O1 - genetics</subject><subject>Vibrio cholerae O1 - pathogenicity</subject><subject>Virulence</subject><issn>0923-2508</issn><issn>1769-7123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1TAQhS1ERW8Lb4CQN7DLxXZ-nGyQUH8AqVI3LVvLdia9c0nsYPtW9Dl44TrkCnasZjT65ujoHELecrbljDcf99sAcUK7FYy1-bTN4wXZcNl0heSifEk2rBNlIWrWnpKzGPeM8VrK6hU55R0Tbd10G_L7EhKECZ1O6B31Aw0w_tnjDudI9eTdA3XeFcn_wgdwaOl3NAE9tTs_QtBAbzk16NPTDPRqpHc-0JiCRhfpEPxEd_4Q4QfAjFkpKwCN8PMAzkKWdz0NaNbnWafsxcXX5GTQY4Q3x3lO7q-v7i6-Fje3X75dfL4pbNXIVPCyrktje6uN4d1QdrKykrVDqysGpTZci-XeG2Gh5g2UragYl0xLM-icTHlOPqy6c_DZUExqwmhhHLWD7FkJJkvR8iaD1Qra4GMMMKg54KTDk-JMLWWovVrLUEsZyzWP_PbuqH8wE_T_no7pZ-D9EdDR6nEI2lmMfznBWduKps7cp5WDnMYjQlDR4hJgjwFsUr3H_zt5BmPNrbU</recordid><startdate>20090101</startdate><enddate>20090101</enddate><creator>Mohapatra, Saswat S.</creator><creator>Ramachandran, Dhanya</creator><creator>Mantri, Chinmay K.</creator><creator>Colwell, Rita R.</creator><creator>Singh, Durg V.</creator><general>Elsevier SAS</general><general>Elsevier Masson</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20090101</creationdate><title>Determination of relationships among non-toxigenic Vibrio cholerae O1 biotype El Tor strains from housekeeping gene sequences and ribotype patterns</title><author>Mohapatra, Saswat S. ; Ramachandran, Dhanya ; Mantri, Chinmay K. ; Colwell, Rita R. ; Singh, Durg V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-13553bcdcabb19f3974c708f8a40e3ab1a2b19fdb2ce516e38240170a7bfa9233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA Polymerase III - genetics</topic><topic>DNA, Bacterial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetic Variation</topic><topic>Housekeeping genes</topic><topic>Malate Dehydrogenase - genetics</topic><topic>Marine</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Phylogeny</topic><topic>Rec A Recombinases - genetics</topic><topic>Recombination, Genetic</topic><topic>Ribotyping</topic><topic>Sequence Analysis, DNA</topic><topic>Sequencing</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae O1 - classification</topic><topic>Vibrio cholerae O1 - genetics</topic><topic>Vibrio cholerae O1 - pathogenicity</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohapatra, Saswat S.</creatorcontrib><creatorcontrib>Ramachandran, Dhanya</creatorcontrib><creatorcontrib>Mantri, Chinmay K.</creatorcontrib><creatorcontrib>Colwell, Rita R.</creatorcontrib><creatorcontrib>Singh, Durg V.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Research in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohapatra, Saswat S.</au><au>Ramachandran, Dhanya</au><au>Mantri, Chinmay K.</au><au>Colwell, Rita R.</au><au>Singh, Durg V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of relationships among non-toxigenic Vibrio cholerae O1 biotype El Tor strains from housekeeping gene sequences and ribotype patterns</atitle><jtitle>Research in microbiology</jtitle><addtitle>Res Microbiol</addtitle><date>2009-01-01</date><risdate>2009</risdate><volume>160</volume><issue>1</issue><spage>57</spage><epage>62</epage><pages>57-62</pages><issn>0923-2508</issn><eissn>1769-7123</eissn><abstract>Sequencing of three housekeeping genes,
mdh,
dnaE and
recA, and ribotyping for seven non-toxigenic
Vibrio cholerae O1 strains isolated from different geographic sources indicate a phylogenetic relationship among the strains. Results of MLST and ribotyping indicate a clear difference between three toxigenic strains (N16961, O395, and 569B) and three non-toxigenic strains from India (GS1, GS2, and GW87) and one Guam strain (X392), the latter of which were similar in both MLST and ribotyping, while two other non-toxigenic strains from the USA and India (2740-80 and OR69) appeared to be more closely related to toxigenic strains than to non-toxigenic strains, although this was not supported by ribotyping. These results provide clues to the emergence of toxigenic strains from a non-toxigenic progenitor by acquisition of virulence gene clusters. Results of split decomposition analysis suggest that widespread recombination occurs among the three housekeeping genes and that recombination plays an important role in the emergence of toxigenic strains of
V. cholerae O1.</abstract><cop>Issy-les-Moulineaux</cop><pub>Elsevier SAS</pub><pmid>19028569</pmid><doi>10.1016/j.resmic.2008.10.008</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Research in microbiology, 2009-01, Vol.160 (1), p.57-62 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacteriology Base Sequence Biological and medical sciences DNA Polymerase III - genetics DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic Variation Housekeeping genes Malate Dehydrogenase - genetics Marine Microbiology Miscellaneous Molecular Sequence Data Multigene Family Phylogeny Rec A Recombinases - genetics Recombination, Genetic Ribotyping Sequence Analysis, DNA Sequencing Vibrio cholerae Vibrio cholerae O1 - classification Vibrio cholerae O1 - genetics Vibrio cholerae O1 - pathogenicity Virulence |
| title | Determination of relationships among non-toxigenic Vibrio cholerae O1 biotype El Tor strains from housekeeping gene sequences and ribotype patterns |
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